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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
ILG Nurtjahtjaningsih
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

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KARAKTERISASI KERAGAMAN GENETIK POPULASI JABON PUTIH MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA ILG Nurtjahtjaningsih; Maryatul Qiptiyah; Tri Pamungkas Yudohartono; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 2 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.2.81-92

Abstract

Anthocepalus cadamba (white jabon) has high economical value for furniture. White jabon forests severely degraded due to intensive exploitation and land conversion. Genetic diversity is one of important consideration to design conservation and improvement strategies. Aim of this study was to access the genetic diversity values within and among population of white jabon. Leaf samples of white jabon were collected from conservation plots originated from West Lombok, Sumbawa, South Sumatera and West Sumatera. Red jabon was included as an outgroup population. Based on 37 polymorphic RAPD loci, the results showed comparable value of genetic diversity between white jabon and red jabon. Number of detected and rare alleles was highest founded in Sumbawa population among the other three populations of white jabon. As consequence, value of expected heterozygosity in the population was highest (HE=0.315). Private allele was only detected in South Sumatera population. Principal coordinate analysis (PCA) showed that integrating between genetic and geographical distance was inconsistent; similar gene resources or human impact might be responsible for this result. The populations that have high value of genetic diversity and private allele are recommended to be selected for the conservation strategies, i.e. Sumbawa and South Sumatera.
KARAKTERISASI DAN APLIKASI PENANDA MIKROSATELIT PADA BEBERAPA Species Eucalyptus ILG Nurtjahtjaningsih; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 2 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2013.7.2.107-118

Abstract

Genetically pure species that used as genetic materials represent crucial factors for succeed of a tree improvement strategy. Using microsatellite markers, private allele and genetic variation could genetically distinguish a species. Aims in this study were to  characterize microsatellite markers on Eucalyptus deglupta, E. urophylla and E. pellita, and to assess private allele and genetic variation on the tree Eucalyptus. Results showed that 8, 10 and 12 out of 13 the screened microsatellite markers were amplified and polymorphic on E. deglupta, E. urophylla and E. pellita respectively. Private alleles characterized each  Eucalyptus. Number of detected allele ranged between 29 (E. deglupta) and 91 (E. pellita). Value of expected heterozygosity was lowest on E. deglupta (HE=0.308) and highest on E. pellita (HE=0.604). Coefficient inbreeding value was insignificant deviate from HWE on E. deglupta and E. urophylla, but it was significant on E. pellita. Taxonomy relationship and geographic position in natural distribution each Eucalyptus was discussed. For further study, population genetic and mating system will be important information on the Eucalyptus.
Co-Authors Anto Rimbawanto AYPBC Widyatmoko Maryatul Qiptiyah Tri Pamungkas Yudohartono