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All Journal JURNAL PEMULIAAN TANAMAN HUTAN
AYPBC Widyatmoko
Balai Besar Penelitian Bioteknologi dan Pemuliaan Tanaman Hutan Yogyakarta
Agriculture, Biological Sciences & Forestry

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SCREENING PENANDA MIKROSATELIT Shorea curtisii TERHADAP JENIS-JENIS SHOREA PENGHASIL TENGKAWANG ILG Nurtjahjaningsih; AYPBC Widyatmoko; Purnamila Sulistyawati; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 1 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.1.49-56

Abstract

Screening primer is an effective method to develop microsatellite markers from related taxa. Aim of this study was to develop microsatellite markers of four Shorea producing tengkawang oil, i.e. Shorea gysbertiana, Shorea macrophylla, Shorea pinanga and Shorea stenoptera by screening microsatellite primers of Shorea curtisii. Leaf samples of the four Shorea were collected from nursery at Center for Forest Biotechnology and Tree Improvement Research in Yogyakarta. Four microsatellite primers of S. curtisii i.e. Shc-1, Shc-2, Shc-7 and Shc-9 had been used to screen. Results showed that numerous alleles were shared among the Shorea. The expected heterozygosity (HE) for locus Shc-1 ranged between 0.594 and 0.722; locus Shc-2 ranged between 0.219 and 0.611; locus Shc-7 ranged between 0.594 and 0.778; and locus Shc-9 ranged between 0.594 and 0.844. Coefficient of inbreeding (FIS) value was low and it was insignificant deviated from Hardy-Weinberg Equilibrium (HWE) at  almost all loci except Shc-1 of S.  pinanga. A dendrogram showed two clusters; S. gysbertsiana and S. macrophylla represented in one cluster, while S. pinanga and S. stenoptera represented in another cluster. Therefore the developed microsatellite markers are possible to be applied for studying population genetics and mating system of these species.
Keragaman Genetik Lima Populasi Gyrinops verstegii di Lombok menggunakan Penanda RAPD AYPBC Widyatmoko; Rizki Diah Afritanti; Taryono Taryono; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 3, No 1 (2009): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2009.3.1.1-10

Abstract

Gyrinops verstegii is one of the species which produce high economic value of gaharu (agarwood). The objectives of this research were to estimate genetic diversity within and among populations, investigate distribution of genetic diversity, and genetic relationship among five populations of G. Verstegii. Leaf samples were taken from five populations of G. Vertegii in Lombok, NTB,. Genetic diversity was analyzed using 21 selective primers out 200 primers. A total of 42 polymorphic loci were detected. Mean of genetig diversity was 0.288, and genetic distance betwen populations was 0.148. The Proportion of 85.24 % of genetic diversity was distributed within population, and the remaining 14.76 % was distributed between populations. Based on cluster analysis, the five populations were separated into two major group consisted of Pusuk Pohon Induk, Pusuk Sumber Benih, and Mantang I. The second group consisted of Mantang Pohon Induk and Mantang II. Collecting genetic materials for conservation and tree improvement program should be focused on the materials within population and each group (regent) should be a representative of at least one population.
Keragaman Genetik dan Hubungan Kekerabatan pada Tiga Jenis Aquilatria Menggunakan Penanda RAPD AYPBC Widyatmoko; Elsih Dian Ariningsih; Aniek Prasetyaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 5, No 3 (2011): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2011.5.3.139-148

Abstract

Gaharu is an imprortant species of non wood forest product of Aquilaria spp with hight economic value. The natural stand has been exploited very intensive. The aim of the study were to investigate level of Aquilaria (namely A. malaccensis, a. beccariana and A. microcarpa) in order to provide genetic information for developing conservation strategy of the species. Aamples were colleted from 7 populations and analyzed using 26 RAPD primers. Total of 84 polymorpic loci have been obtained from these primers. Mean of genetic diversity within population indicated by A. malaccensis Muara Bungo population. A. malaccensis Berau population has the lowest genetic diversity. The highest genetic distance between populations was between A. micricarpa samboja and A. beccariana Berau. In contrast, genetic distance between A. Microcarpa Bearu and A. malaccensis Berau was the the lowest (0.017). based on Cluster analysis, The seven populations were divided into 2 groups. The first group consisted of 2 populations of A. malaccensis and A. microcarpa from Kalimantan.
KARAKTERISASI KERAGAMAN GENETIK POPULASI JABON PUTIH MENGGUNAKAN PENANDA RANDOM AMPLIFIED POLYMORPHISM DNA ILG Nurtjahtjaningsih; Maryatul Qiptiyah; Tri Pamungkas Yudohartono; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 2 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.2.81-92

Abstract

Anthocepalus cadamba (white jabon) has high economical value for furniture. White jabon forests severely degraded due to intensive exploitation and land conversion. Genetic diversity is one of important consideration to design conservation and improvement strategies. Aim of this study was to access the genetic diversity values within and among population of white jabon. Leaf samples of white jabon were collected from conservation plots originated from West Lombok, Sumbawa, South Sumatera and West Sumatera. Red jabon was included as an outgroup population. Based on 37 polymorphic RAPD loci, the results showed comparable value of genetic diversity between white jabon and red jabon. Number of detected and rare alleles was highest founded in Sumbawa population among the other three populations of white jabon. As consequence, value of expected heterozygosity in the population was highest (HE=0.315). Private allele was only detected in South Sumatera population. Principal coordinate analysis (PCA) showed that integrating between genetic and geographical distance was inconsistent; similar gene resources or human impact might be responsible for this result. The populations that have high value of genetic diversity and private allele are recommended to be selected for the conservation strategies, i.e. Sumbawa and South Sumatera.
HUBUNGAN KEKERABATAN ANTAR POPULASI JATI (Tectona Grandis,Linn.F.) BERDASARKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) AYPBC Widyatmoko; Anto Rimbawanto; Abdul Razaq Chasani
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 3 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2013.7.3.151-166

Abstract

Information on genetic relationship amongst teak (Tectona grandis Linn.f) populations in Indonesia is important, in order to maintain the high genetic diversity of the species and to ensure the origin of commercial planting materials. In this study, 94 loci from 25 RAPD primers were used for analyzing genetic diversity and genetic relationship among 30 populations of teak which collected from provenance trial in Bojonegoro and its populations in Sulawesi. Mean genetic diversity (h) of the 30 populations was 0.184, and mean genetic distance between populations (D) was 0.441. Based on cluster analysis, 30 populations of teak were divided into 3 groups. The first group consisted of Burma, the second group consisted of Jawa, India, Indochina and Thailand populations, and the third group consisted of all  populations in Sulawesi. High genetic distance between Jawa’s and Sulawesi’s populations can be used to  differentiate seed/seedling from both regions.
KARAKTERISTIK PEMBUNGAAN DAN SISTEM PERKAWINAN NYAMPLUNG (Calophyllum inophyllum) PADA HUTAN TANAMAN DI WATUSIPAT, GUNUNG KIDUL ILG Nurtjahjaningsih; Purnamila Sulistyawati; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 6, No 2 (2012): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2012.6.2.65-78

Abstract

Flowering are influenced by internal factors, such as genetic and phytohormone, and environment factors, such as sunlight and nutrition intake. The flowering characteristics influence fruiting and genetic diversity seedlings through mating systems. This study aims to assess flowering and fruiting characteristics and to determine pattern of mating system of a Calophyllum inophyllum plantation at Watusipat, Gunung Kidul. Flowering and fruiting were observed at 4 locations, 3 parts of crown, and 4 main directions to know the effects of sunlight, nutrition intake and phytohormone in the flowering process. Mating system was assessed by comparing genetic diversity values between parent trees and offsprings. The values of genetic diversity were analyzed using 5 RAPD primers with 17 polymorphic loci. Analysis of variant showed that the locations, crown parts, directions and interaction between a location and direction significantly affected to differences number of flowers and fruits. Values of genetic diversity (h) of parent trees ranged between 0.1471 and 0.3056. The values increased at almost overall offsprings; it ranged between 0.2864 and 0.3750. Values of genetic distance (Da) between parent trees were high and very high (0.197 – 0.364), but the values was decreased between parent trees and their offspring, even between offspring populations. A dendrogram showed two main clusters; first cluster consisted parent trees at up edge with rare trees and second cluster consisted sub cluster parent trees at up edge; sub cluster parent trees at down middle; and sub cluster parent trees at down edge and overall offsprings. Flowering/ fruiting characteristics and pattern of mating systems of C. inophyllum were briefly discussed. Key words : Flowering characteristic, genetic diversity, Calophyllum inophyllum, RAPD analysis
KERAGAMAN GENETIK ANAKAN Shorea leprosula BERDASARKAN PENANDA MIKROSATELIT Purnamila Sulistyawati; AYPBC Widyatmoko; ILG Nurtjahjaningsih
Jurnal Pemuliaan Tanaman Hutan Vol 8, No 3 (2014): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2014.8.3.171-183

Abstract

Genetic diversity value of offspring might indicate a reproductive success in a forest. Aim in this study was to access genetic diversity values of offspring of Shorea leprosula from different forest types. Leaf samples were collected from six population i.e. a plantation from Carita, and five natural forests from Gunung Bunga A and B, SBK, ITCI and Gunung Lumut. Using four microsatellite markers, the results showed that SBK population maintained high value of genetic diversity. Values of expected heterozygosity (HE) ranged between 0.717 (Carita) and 0.836 (SBK). Values of coefficient inbreeding (F) were insignificant deviated from Hardy-Weinberg Equilibrium, but the value was significant in SBK population. PCA analysis demonstrated a cluster among Gunung Bunga A, SBK, ITCI, and Gunung Lumut. Amova showed that different province significantly contributed 1% to the value of genetic diversity of S. leprosula.
KARAKTERISASI DAN APLIKASI PENANDA MIKROSATELIT PADA BEBERAPA Species Eucalyptus ILG Nurtjahtjaningsih; AYPBC Widyatmoko; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 7, No 2 (2013): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2013.7.2.107-118

Abstract

Genetically pure species that used as genetic materials represent crucial factors for succeed of a tree improvement strategy. Using microsatellite markers, private allele and genetic variation could genetically distinguish a species. Aims in this study were to  characterize microsatellite markers on Eucalyptus deglupta, E. urophylla and E. pellita, and to assess private allele and genetic variation on the tree Eucalyptus. Results showed that 8, 10 and 12 out of 13 the screened microsatellite markers were amplified and polymorphic on E. deglupta, E. urophylla and E. pellita respectively. Private alleles characterized each  Eucalyptus. Number of detected allele ranged between 29 (E. deglupta) and 91 (E. pellita). Value of expected heterozygosity was lowest on E. deglupta (HE=0.308) and highest on E. pellita (HE=0.604). Coefficient inbreeding value was insignificant deviate from HWE on E. deglupta and E. urophylla, but it was significant on E. pellita. Taxonomy relationship and geographic position in natural distribution each Eucalyptus was discussed. For further study, population genetic and mating system will be important information on the Eucalyptus.
KERAGAMAN GENETIK POPULASI Araucaria cunninghamii MENGGUNAKAN PENANDA RAPD (RANDOM AMPLIFIED POLYMORPHIC DNA) AYPBC Widyatmoko; Elisabeth Selda Patrisia Lejo; Aniek Prasetyaningsih; Anto Rimbawanto
Jurnal Pemuliaan Tanaman Hutan Vol 4, No 2 (2010): Jurnal Pemuliaan Tanaman Hutan
Publisher : Center for Forest Biotechnology and Tree Improvement (CFBTI)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/jpth.2010.4.2.63-77

Abstract

Genetic diversity was needed for supporting genetic conservation and tree improvement program. Araucaria  cunnighamii is one of Indonesian conifer species which has various potential uses. Information about genetic diversity and its distribution, and genetic relationship can be revealed using RAPD (Random Amplified Polymorphic DNA) molecular marker. The objective of the study was to analyze genetic diversity within and among 8 populations of A. Cunninghamii, and genetic relationship between populations. Leaf samples of 64 trees were collected from 7 population in Papua and 1 population in Queensland (Australia). Genetic diversity was analyzed using 23 RAPD primers with 68 polymorphic loci. Mean genetic diversity within population was 0.270, and mean genetic distance among populations was 0.092. Some of the samples from the same population were grouped in the same cluster. But generally, the clustering of the samples. Genetic relationship among A. cunninghamii populations revealed that 8 populations were divided into two groups. First group was consisted of 7 population in Papua (Indonesia) and then split into 5 subcluster. Second group was population in Queensland (Australia).
Co-Authors Abdul Razaq Chasani Aniek Prasetyaningsih Aniek Prasetyaningsih Anto Rimbawanto Anto Rimbawanto Elisabeth Selda Patrisia Lejo Elsih Dian Ariningsih ILG Nurtjahjaningsih ILG Nurtjahtjaningsih Maryatul Qiptiyah Purnamila Sulistyawati Rizki Diah Afritanti Taryono, Taryono Tri Pamungkas Yudohartono