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UJI AKTIVITAS ANTIOKSIDAN FRAKSI ETIL ASETAT DAUN WUNGU (Graptophyllum pictum (Linn) Griff) DENGAN METODE FRAP (FERRIC REDUCING ANTIOXIDANT POWER) Aminah Aminah; A. Muflihunna; Zainal Abidin
As-Syifaa Jurnal Farmasi Vol 8, No 1 (2016): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.989 KB) | DOI: 10.33096/jifa.v8i1.156

Abstract

‘Wungu’ leaf is a plant known to contain flavonoids as the antioxidant. Thisresearch aimed to determine the antioxidant activit ethyl acetace fraction of ‘Wungu’ leaf (Graptophyllum pictum  (Linn) Griff) by Ferric Reducing Antioxidants Power (FRAP) method. Extraction by maceration method usingethanol 96 %. Extract partitioned with ethyl acetace and water. Thefiltrate was dried with rotary evaporator and produce ethyl acetace fraction.Fraction were analyzed quantitatively by FRAP method with potassium ferriccyanide reagent solution, phosphate buffer, ferricchloride and distillede water. The sampel solutions analyzed by UV-vis spectrophotometer at a maximum wavelength of 705 nm using kuarsetin solution as a standard. The result showed that the antioxidantactivity of ethyl acetate fraction of ‘wungu’(Graptophyllum pictum  (Linn) Griff) is3.984x10-6 g QE/g fraction.
ANALISIS AKTIVITAS ANTIOKSIDAN SEDIAAN PROPOLIS YANG BEREDAR DI KOTA MAKASSAR DENGAN METODE FRAP (Ferric Reducing Antioxidant Power) Mamat Pratama; A. Muflihunna; Nurazizah Octaviani
As-Syifaa Jurnal Farmasi Vol 10, No 1 (2018): AS-SYIFAA Jurnal Farmasi
Publisher : Fakultas Farmasi UMI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (257.303 KB) | DOI: 10.33096/jifa.v10i1.312

Abstract

Antioxidants are chemical compounds that can donate one or more electrons to free radical, so that free radicals that can be suppressed.This study aimed to obtain and provide scientific evidence to the public regarding the antioxidant activity in propolis liquid product circulating in Makassar. This study using FRAP (Ferric Reducing Antioxidant Power) and 96% ethanol. Free radical activity absorbance was measured with a  UV-VIS spectrophotometer at a wavelength of 710 nm and the total value of antioxidant activity was calculated based on data absorbance. The calculations show that the sample A has antioxidant activity of 0.0971 mgAAE / mg sample, the sample B has antioxidant activity of 0.0669 mgAAE / mg sample, the sample C has antioxidant activity of 0.0493 mgAAE / mg samples.