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All Journal Agrointek BERITA BIOLOGI
Harto Widodo
Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

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PENANGANAN DAN PENERAPAN TEKNOLOGI PASCAPANEN TANAMAN OBAT Harto Widodo; Dyah Subositi
AGROINTEK Vol 15, No 1 (2021)
Publisher : Agroindustrial Technology, University of Trunojoyo Madura

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21107/agrointek.v15i1.7661

Abstract

Post-harvest handling of medicinal plants (MP) is an important process for providing medicinal materials which either directly used as a simplicia or further processed products. Improper handling will deteriorate the physical, chemical and biological (microbial contamination) quality of simplicia. Otherwise, the appropriate process will achieve good quality products especially the target active constituents of medicinal plants that can be maintained, or even increased. The purpose of this paper was to discuss the important aspects of postharvest management and the application of technology to reduce the loss of quality and quantity of medicinal plant yields. The post-harvest process of medicinal plants generally includes the stages of fresh sorting, washing, draining, cutting, drying, dry sorting, packaging and storing. The principle of postharvest handling of medicinal plants must be based on obtaining the product with the lowest possible cost, water content, energy consumption, space, and resources, and with the highest physical, chemical, and biological quality. The best postharvest handling methods for one medicinal plant cannot be generally applied to other medicinal plants. Proper post-harvest technologies should be developed for an individual MP to achieve quality products. Comprehensive research on postharvest handling process is needed for individual MP commodity based on the characteristics of each ingredient and the target of active principles so that the most suitable method for each type of medicinal plant is obtained.
KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR) Dyah Subositi; Harto Widodo
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i2.2642

Abstract

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5–90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants. 
Co-Authors Dyah Subositi Dyah Subositi