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All Journal BERITA BIOLOGI Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal
Dyah Subositi
Balai Besar Penelitian dan Pengembangan Tanaman Obat dan Obat Tradisional
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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KERAGAMAN GENETIK ALANG-ALANG (Imperata cylindrica (L.) Beauv.) BERDASARKAN MARKA INTER-SIMPLE SEQUENCE REPEATS (ISSR) Dyah Subositi; Harto Widodo
BERITA BIOLOGI Vol 17, No 2 (2018)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v17i2.2642

Abstract

Alang-alang (Imperata cylindrica (L.) Beauv.) has been widely used as a medicinal plant to treat some diseases, such as fever, headache, and diuretic. Nowadays, there is no information of genetic diversity of this plant used in herbal formula by ethnic groups in Indonesia. The main objective of this study was to asses genetic diversity of alang-alang from 18 selected ethnic groups in Indonesia based on Inter Simple Sequence Repeats (ISSR). Location of sample collection was identified by using data on Research on Medicinal Plant (Ristoja) 2012. Total DNA genome was isolated and ISSR primer screening were done on collected samples. Ten selected ISSR primers produced 74 amplified DNA fragments 58, fragments (78.4 %) were polymorphic. Dice index similarity was used to construct UPGMA dendrogram. The genetic similarity indexing which among accessions was ranged from 70.5–90.5% thereby indicating a low level of genetic diversity occurred in alang-alang. The results of this study also showed that ISSR markers were able to genetically differentiate alang-alang accessions by which this information can be useful for further researchs such as for standardization of medicinal plants. 
Keanekaragaman Genetik Tempuyung (Sonchus arvensis L.) Berdasarkan Marka Inter-Simple Sequence Repeats (ISSR) Dyah Subositi; Rohmat Mujahid
Majalah Ilmiah Biologi BIOSFERA: A Scientific Journal Vol 36, No 2 (2019)
Publisher : Fakultas Biologi | Universitas Jenderal Soedirman

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20884/1.mib.2019.36.2.828

Abstract

Tempuyung (Sonchus arvensis L.) merupakan tanaman anggota familia Asteraceae yang banyak dimanfaatkan sebagai tumbuhan obat untuk menurunkan tekanan darah tinggi dan peluruh air seni. Tempuyung mudah dan banyak dijumpai di berbagai tempat di Indonesia. Keanekaragaman genetik tempuyung merupakan informasi dasar dalam rangka mendukung standarisasi tumbuhan obat. Penelitian ini bertujuan untuk melakukan karakterisasi genetik tempuyung menggunakan penanda molekuler ISSR (Inter Simple Sequence Repeats). Tiga belas aksesi tempuyung yang dikoleksi dari 8 lokasi digunakan sebagai sampel dan diamplifikasi DNAnya menggunakan 5 primer ISSR.Indeks similaritas dihitung berdasarkan indeks similaritas Dice. Analisis klaster menggunakan metode Unweighted Pair Group Method Using Arithmetic Mean (UPGMA) dilakukan untuk mengonstruksi dendogram. Hasil penelitian menunjukkan adanya variasi genetik antar aksesi tempuyung yang terbagi menjadi 3 klaster pada indeks similaritas 75,16%. Aksesi Purwokerto dan B2P2TOOT (4) mempunyai hubungan kemiripan yang terdekat pada indeks similaritas 90,90%. Penanda molekuler ISSR dapat digunakan dalam karakterisasi genetik antara aksesi tempuyung
Co-Authors Aniska Novita Sari Fitriana Fitriana Harto Widodo Inge Oktaviani Rohmat Mujahid