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POTENSI JAMUR ENTOMOPATOGEN Metarhizium anisopliae, Beauveria bassiana dan Streptomyces sp. TERHADAP MORTALITAS Lepidiota stigma PADA TANAMAN TEBU Aditya R. Hidayah; Wiwik Sri Harijani; Wiludjeng Widajati; Dina Ernawati
Plumula : Berkala Ilmiah Agroteknologi Vol 7 No 2 (2019): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Sugarcane is a plant which only grow in tropical. Plant pests and plant diseases in cultivation have never been separated. One of the important pests of sugarcane is uret root destroyers (Lepidiota stigma). The pests can reduce crop yields by 50%. One of the best alternatives for uret pest control which can be safety and environmentally friendly by using biological agents. The biological agents for pests control has a good potential and prospects because they are host-specific and harmless to human. This study was conducted to determine the effectiveness of Metarhizium anisopliae, Beauveria bassiana and Streptomyces sp.mortality Lepidiota stigma. This study used 3 treatments in a completely randomized design (CRD) with each repeated 9 times. The results showed that for four observations at intervals of seven days of observation. The ability of three biological agents has different varieties in turning off the test insect, this is indicated by the highest mortality percentage of M. anisopliae with total mortality of 88.89%.

PENGARUH INSEKTISIDA MIKROBA Bacillus thuringiensis TERHADAP MORTALITAS Heliothis armigera PADA TONGKOL JAGUNG Wiludjeng Widajati; Wiwin Windriyanti; Wahyu Santoso
Plumula : Berkala Ilmiah Agroteknologi Vol 8 No 1 (2020): Plumula : Berkala Ilmiah Agroteknologi
Publisher : UPN VETERAN JAWA TIMUR

Crop of corn (Zea mays L.) is seed crop which be used developed nations as staple food after rice. Pest activatir of cob of Heliothis armigera Hubner represents one of the corn crop pest which destroys many corn fruit especially at young fruit. The progress of technology has found a way to eradicate H. armigera biologically buy using a certain organism which is formulated in the form of pesticides. One of the product is Bactospeine WP which contains of active materials of Bacillus thuringiensis Berliner. The purpose of this research is to know the percentage of death symptom influenced by Bactospeine WP as stomach poison to some larva H. armigera at various given concentration. The research is conducted on laboratory. The research method used is Complete Random Device (CRD) with two factors and four times repetition. The first factor is concetration with three levels and second factor is larva instar with four levels. Perception is conducted on 1st, 2nd, 3rd, 4th and 5th day after treatment. Result of research indicates that death of larva H. armigera can be influenced by different of concetration of Bactospeine WP and instar larva.

Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.

The Presence of Soybean Plant Insects (Glycines Max L. Merril) in the Vegetative Phase with the Application Treatment of Streptomyces sp. and Trichoderma sp. Nugraha Iqbal Avrianto; Penta Suryaminarsih; Wiludjeng Widajati
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2005

Soybean (Glycine max (L.) Merill) is one of the main food crops in the community. In East Java, productivity has declined due to various factors, including pest attacks. This study aims to determine the diversity of insects by administering a mixture of biological control agents (BSAs) between Streptomyces sp. and Trichoderma sp. as an entomopathogen applied to soybean plants in the vegetative phase. The soybean plants were planted in plastic bags on the ground of the Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran Jawa Timur. The aim of this study was to determine the effect of Streptomyces sp. and Trichoderma sp. the type and population of parasites. This study used a randomized two-way block design of 13 treatments repeated 3 times. Based on the observations, the total number of insects found in the experimental field of UPN veterans Jawa Timur was 2,360 individuals, made up of six orders of insects. The four orders are divided into 8 families and 8 species. Hemiptera species became the most dominant order (2202 individuals; 93%), Orthoptera (108 individuals; 5%), Lepidoptera (18 individuals; 1%) and Diptera (32 individuals; 1%) . The use of Streptomyces sp. and Trichoderma sp. the single comparison treatment is more effective than the combined comparison on the pest population. The main insects include Aphid spp, Bemisia tabaci and Nezera viridula.

PENGARUH INSEKTISIDA MIKROBA Bacillus thuringiensis TERHADAP MORTALITAS Heliothis armigera PADA TONGKOL JAGUNG Wiludjeng Widajati; Wiwin Windriyanti; Wahyu Santoso
Plumula : Berkala Ilmiah Agroteknologi Vol. 8 No. 1 (2020): Plumula : Berkala Ilmiah Agroteknologi
Publisher : Agrotechnology Study Program, UPN "Veteran" Jawa Timur

Show Abstract | Download Original | Original Source | Check in Google Scholar

Tanaman jagung (Zea mays L.) merupakan tanaman benih yang dimanfaatkan negara berkembang sebagai bahan pangan pokok setelah padi. Aktivasi hama tongkol Heliothis armigera Hubner merupakan salah satu hama tanaman jagung yang banyak merusak buah jagung terutama pada buah muda. Kemajuan teknologi telah menemukan cara untuk membasmi H. armigera yang dibeli secara biologis dengan menggunakan organisme tertentu yang diformulasikan dalam bentuk pestisida. Salah satu produknya adalah Bactospeine WP yang mengandung bahan aktif Bacillus thuringiensis Berliner. Tujuan penelitian ini adalah untuk mengetahui persentase gejala kematian yang dipengaruhi oleh Bactospeine WP sebagai keracunan lambung pada beberapa larva H. armigera pada berbagai konsentrasi yang diberikan. Penelitian dilakukan di laboratorium. Metode penelitian yang digunakan adalah Rancangan Acak Lengkap (CRD) dengan dua faktor dan empat kali pengulangan. Faktor pertama konsentrasi jentik tiga taraf dan faktor kedua instar jentik empat taraf. Persepsi dilakukan pada hari ke-1, ke-2, ke-3, ke-4 dan ke-5 setelah perlakuan. Hasil penelitian menunjukkan bahwa kematian larva H. armigera dapat dipengaruhi oleh perbedaan konsentrasi Bactospeine WP dan larva instar.

Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.

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