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All Journal Plumula : Berkala Ilmiah Agroteknologi Nusantara Science and Technology Proceedings
Wiludjeng Widajati
Agrotechnology Study Program, Agriculture Faculty, Universitas Pembangunan Nasional“Veteran” Jawa Timur, Surabaya 60294, Indonesia
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Energy Engineering Immunology & microbiology Law, Crime, Criminology & Criminal Justice Materials Science & Nanotechnology Medicine & Pharmacology

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Journal : Nusantara Science and Technology Proceedings

 1 
Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Abstract

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.
The Presence of Soybean Plant Insects (Glycines Max L. Merril) in the Vegetative Phase with the Application Treatment of Streptomyces sp. and Trichoderma sp. Nugraha Iqbal Avrianto; Penta Suryaminarsih; Wiludjeng Widajati
Nusantara Science and Technology Proceedings Seminar Nasional Agroteknologi Fakultas Pertanian UPN “Veteran” Jawa Timur 2021
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2022.2005

Abstract

Soybean (Glycine max (L.) Merill) is one of the main food crops in the community. In East Java, productivity has declined due to various factors, including pest attacks. This study aims to determine the diversity of insects by administering a mixture of biological control agents (BSAs) between Streptomyces sp. and Trichoderma sp. as an entomopathogen applied to soybean plants in the vegetative phase. The soybean plants were planted in plastic bags on the ground of the Faculty of Agriculture, Universitas Pembangunan Nasional “Veteran Jawa Timur. The aim of this study was to determine the effect of Streptomyces sp. and Trichoderma sp. the type and population of parasites. This study used a randomized two-way block design of 13 treatments repeated 3 times. Based on the observations, the total number of insects found in the experimental field of UPN veterans Jawa Timur was 2,360 individuals, made up of six orders of insects. The four orders are divided into 8 families and 8 species. Hemiptera species became the most dominant order (2202 individuals; 93%), Orthoptera (108 individuals; 5%), Lepidoptera (18 individuals; 1%) and Diptera (32 individuals; 1%) . The use of Streptomyces sp. and Trichoderma sp. the single comparison treatment is more effective than the combined comparison on the pest population. The main insects include Aphid spp, Bemisia tabaci and Nezera viridula.
Pathogenicity of Entomopathogenic Nematodes (NEP) Results of In Vitro Propagation Using Egg Yolk Media Against Mustard Caterpillar Plutella xylostella Mohamad Hipti; Wiludjeng Widajati; Sri Wiyatiningsih; Ramadhani Mahendra Kusuma
Nusantara Science and Technology Proceedings Sains dan Teknologi Pertanian Modern
Publisher : Future Science

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.11594/nstp.2021.1509

Abstract

Decreased mustard plant production is inseparable from the presence of plant pests and disease, one of which is Plutella xylostella. This pest can reduce the yield by 30-40%, even in some cases found to experience crop failure. Generally, in the community, controlling pests is using chemical pesticides. However, excessive use of chemical pesticides harms the environment and triggers health problems in humans, So, biological control is needed. One of them is an entomopathogenic nematode (NEP). Advantages Nematodes are environmentally friendly and are fast in crippling their host, but the multiplication of entomopathogenic nematodes encounters many obstacles, so research on these alternatives is needed. This research was carried out to determine the pathogenicity of entomopathogenic Nematodes cultured from the egg yolk media on Plutella xylostella larvae, then developed as an effective biocontrol. The pathogenicity test for Plutella xylostella larvae was performed using nine treatments placed in a factorial Completely Randomized Design (CRD) with each treatment repeated three times. The study indicate that the four bacterial isolates had the same ability to kill Plutella xylostella larvae during four days of observation, with the greatest percentage of 100 percent and the lowest percentage of 76.7 percent.
Co-Authors Aditya R. Hidayah Dina Ernawati Mohamad Hipti Nugraha Iqbal Avrianto Penta SURYAMINARSIH Ramadhani Mahendra Kusuma Sri Wiyatiningsih Wahyu Santoso Windriyanti, Wiwin Wiwik Sri Harijani