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All Journal Journal of Innovation and Applied Technology The Indonesian Green Technology Journal The Journal of Pure and Applied Chemistry Research INDONESIAN JOURNAL OF ESSENTIAL OIL Jurnal Penelitian Pendidikan IPA (JPPIPA)
Elvina Dhiaul Iftitah
Brawijaya University
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Earth & Planetary Sciences Education Materials Science & Nanotechnology Medicine & Pharmacology Physics Other

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Journal : INDONESIAN JOURNAL OF ESSENTIAL OIL

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Study of Enzymatic Hydrolysis Reaction l-Menthyl acetate using Lipase Enzyme from Candida antarctica Recombined Aspergillus oryzae by In Vitro and In Silico Yasmin Zafirah; Elvina Dhiaul Iftitah
INDONESIAN JOURNAL OF ESSENTIAL OIL Vol 2, No 2 (2017)
Publisher : Institut Atsiri Universitas Brawijaya

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Abstract

Research about the hydrolysis of l-menthyl acetate catalyzed by lipase enzyme from Candida antarctica recombined Aspergillus oryzae has been observed by in vitro and in silico. The reaction by in vitro method performed with variations time and solvent. The result obtained that lipase enzyme from Candida antarctica recombined Aspergillus oryzae were able to catalyze the hydrolysis reaction of l-menthyl acetate to produce l-menthol. The best result is observed at 16 hours with ethanol solvent, which give % conversion about 13,76%. The research followed by in silico using the docking method,  observed that mechanism between amino acid residues to macromolecules as well as the influence of solvents on such interactions. The residues that involved in the reaction are Glu, Thr, Asp, Gln, Ser, His. The ethanol solvent shows a good interaction with macromolecules by forming hydrogen bonds.
Co-Authors Adilatunnisa Nur Indah Berliana Edi Priyo Utomo Fadhlih Arya Seta Fitri Ariadna Sodi Miranda Khoirun Nisyak Masruroh Masruroh Mohammad Farid Rahman Qurrotul A'yun Rizki Wahyu Aji Wibowo Siti Mariyah Ulfa Udrika Lailatul Qodri Warsito Warsito Yasmin Zafirah