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Indranila KS
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Aspek Biomolekuler Apoptosis, Caspase-3 & RAK pada Pemberian Morinda Citrifolia L (Mengkudu) Tikus Sprague Dawley Diabetes Nefropati yang Diinduksi Streptocotocin (STZ) Indranila KS
Medica Hospitalia : Journal of Clinical Medicine Vol. 1 No. 3 (2013): Med Hosp
Publisher : RSUP Dr. Kariadi

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (317.479 KB) | DOI: 10.36408/mhjcm.v1i3.63

Abstract

Latar belakang : Nefropati diabetes (ND) adalah salah satu bentuk komplikasi mikrovaskuler yang sering dijumpai pada penderita diabetes mellitus (DM). Morinda citrifolia L (Mengkudu) memiliki komponen zat bioaktif bersifat imunomodulator, imunosupresif, anti inflamasi dan antiapoptosis, diharapkan dapat memperbaiki fungsi ginjal dengan pengukuran biomarker RAK, indeks apoptosis, dan caspase-3. Tujuan penelitian : Membuktikan peran Morinda citrifolia L(MC) dalam memperbaiki fungsi ginjal ND pada tikus SD yang diinduksi STZ. Metode penelitian :The post test only control group desain. Tiga puluh enam ekor tikus Sprague Dawley dibagi menjadi 6 kelompok : 2 kelompok kontrol, dan 4 kelompok perlakuan. Tikus diinduksi STZ dosisi 40 mg/kgBB selama 8 minggu, kemudian di beri MC dosis 10,20,40,80mg/dL selama 2 minggu. Pada akhir penelitian tikus diperiksa RAK, indeks apoptosis, caspase-3. Data dianalisis menggunakan Mann Whitney dan regresi linier berganda. dengan tingkat kemaknaan p<0,05. Hasil dan pembahasan :Pada penelitian ini digunakan 36 ekor tikus SD yang dikelompokkan menjadi 6 kelompok yaitu kelompok 1 mendapat kelompok STZ + MC 10 mg, 1 kelompok mendapat kelompok STZ + MC 20 mg,1 kelompok STZ + MC 40 mg, 1kelompok STZ + MC 80 mg,1 kelompok STZ / kontrol (+ ) dan 1 kelompok kelompok kontrol (-). Hasil uji statistik menunjukkan RAK urin kelompok MC 20 mg adalah lebih rendah secara bermakna dibanding kelompok kontrol (+) dengan nilai p=0,04. Indeks apoptosis kelompok MC 20 mg adalah lebih rendah secara bermakna dibanding kontrol (+) dengan p=0,002. Ekspresi caspase-3 jaringan glomerulus yang tertinggi adalah pada kelompok yang mendapat STZ dan MC yang paling rendah adalah pada kelompok yang mendapat MC 20 mg. Korelasi antara ekspresi caspase-3 dengan indeks apoptosis pada jaringan glomerulus dan tubulus ginjal menunjukkan adanya korelasi positip kuat (p<0,001) Hal ini secara tidak langsung dapat menjelaskan apotosis yang terjadi pada jaringan nefron ginjal adalah melalui jalur caspase. Simpulan dan saran : Pemberian Morinda citrifolia L dosis 10,20,40,80mg/dL dapat menurunkan status albuminuri berdasarkan pengukuran RAK, indeks apoptosis glomerulus, ekspresi caspase-3. Pemberian ekstrak Morinda citrifolia L dapat memperbaiki fungsi ginjal paling bermakna pada konsentrasi 20 mg/dL,dan berpengaruh paling kuat untuk perbaikan ND melalui apoptosis glomerulus terhadap penurunan status albuminuria (RAK).Perlu dilakukan penelitian lanjutan pada manusia, dengan dosis 100mg/kgBB, yang merupakan hasil konversi dosis 20mg/dL pada tikus. Kata kunci : nefropati diabetes, morinda citrifolia L, tikus SD, STZ, apoptosis, caspase-3, RAK
Pengaruh Polifenol Mahkota Dewa Terhadap Proliferasi Sel dan Apoptosis pada Mencit Strain Balb/C yang Diinduksi Benzo(a) Pyrene (BaP) Theopilus W. Watuguly; Indranila KS; Pamela Mercy Papilaya; Edi Dharmana
MEDIA MEDIKA INDONESIANA 2013:MMI VOLUME 47 ISSUE 1 YEAR 2013
Publisher : MEDIA MEDIKA INDONESIANA

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (482.839 KB)

Abstract

ABSTRACT The effect of mahkota dewa polyphenol in the cell proliferation and apoptosis in BaP induced Balb/c ratsBackground: The polyphenol of mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) has the potency as antioxidant and anticancer which can handle free radicals, but there has not been extensive research on this. This research is aimed to prove the role of mahkota dewa polyphenol in the cell proliferation inhibition and induct lung carcinogenesis apoptosis in strain Balb/c mice which inducted with benzo(a)pyrene (BaP).Methods: Posttest control group design was carried out among 40 strain Balb/c mice sample, aged 1-2 weeks, weighed 20-30 grams, healthy mice condition. All mice were inducted with BaP and then randomized into 2 groups, as control group and the treatment group. The development of the lung tumor was observed by tissue surgery in the 8th, 17th and 26th week. The data collected were AgNORs, and IHC-TUNEL-apoptosis index dying. The data analysis was conducted using Kruskal-Wallis, Mann-Whitney, One-way ANOVA, and Post hoc test LSD with significance degree of p<α (0.05).Results: The oral administration of mahkota dewa polyphenol showed significantly decreasing cell proliferation, increasing apoptosis index in treatment group in week 8, 17 and 26 (p=0.000). Carcinogenesis incidence for the treatment group week 8 and 26 were 2.32±0.26 and 3.93±0.46, while for the treatment group were 1.88±0.38 and 0.88±0.22 (p=0.000). The cell proliferations for control group week 8 and 26 were 1.57±0.12 and 2.29±0.15, while for the treatment group were 1.53±0.11 and 1.60±0.04 (p=0.000). Apoptosis index for the control group for week 8 was 0.00±0.00 and 0.92±0.22 in week 26, while the treatment group was 1.12±0.71 and 2.02±1.05 (p=0.000).Conclusion: The administration of mahkota dewa polyphenol effectively inhibited the cell proliferation activity and increased apoptosis measured by apoptosis index. Therefore polyphenol has anticancer and antioxidant activities which can inhibit lung carcinogenesis in Balb/c mice.Keywords: Polyphenol, mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl), apoptosis index, strain Balb/c mice, benzo(a)pyrene (BaP).ABSTRAKLatar belakang: Polifenol mahkota dewa (Phaleria macrocarpa [Scheff.] Boerl) berpotensi sebagai antioksidan dan antikanker yang mampu menangkap radikal bebas, namun belum banyak diteliti. Penelitian ini bertujuan membuktikan peran polifenol mahkota dewa dalam menginhibisi proliferasi sel dan menginduksi apoptosis pada mencit strain Balb/c hasil induksi Benzo(a)pyrene (BaP).Metode: Posttest control group design dengan sampel 40 mencit strain Balb/c, umur 1-2 minggu, berat 20-30 g, kondisi mencit sehat. Semua mencit diinduksi BaP kemudian hewan dirandomisasi menjadi 2 kelompok yaitu kelompok kontrol dan kelompok perlakuan polifenol 50 mg. Perkembangan tumor paru diamati dengan pembedahan jaringan pada minggu ke-8, 17 dan 26. Data dikumpulkan meliputi AgNORs, pengecatan IHC-TUNEL-indeks apoptosis. Analisis data dengan Kruskal-Wallis, Mann-Whitney, One-way ANOVA, post hoc test LSD dengan derajat kemaknaan p<α (0,05).Hasil: Pemberian oral polifenol mahkota dewa sebesar 50 mg secara bermakna memperlihatkan penurunan insidens karsinogenesis paru, proliferasi sel, protein Bax dan peningkatan indeks apoptosis, protein p53, Bcl-2, ekspresi caspase 3, 8, 9 pada kelompok perlakuan pada minggu ke-8, 17 dan 26 (p=0,000). Insidens karsinogenesis untuk kelompok kontrol minggu ke-8, dan 26 sebesar 2,32±0,26 dan 3,93±0,46, sedangkan kelompok perlakuan sebesar 1,88±0,38 dan 0,88±0,22 (p=0,000). Proliferasi sel untuk kelompok kontrol minggu ke-8 dan 26 sebesar 1,57±0,12 dan 2,29±0,15, sedangkan kelompok perlakuan sebesar 1,53±0,11 dan 1,60±0,04 (p=0,000). Indeks apoptosis pada kelompok kontrol minggu ke-8 sebesar 0,00±0,00 dan 0,92±0,22 minggu ke-26, sedangkan kelompok perlakuan sebesar 1,12±0,71 dan 2,02±1,05 (p=0,000).Simpulan: Pemberian polifenol mahkota dewa efektif meng-inhibisi proliferasi sel dan menginduksi apoptosis melalui peningkatan indeks apoptosis. Jadi polifenol mahkota dewa memiliki aktivitas antikanker dan antioksidan mampu menghambat karsinogenesis paru mencit Balb/c.
PROCALCITONIN DAN INTERLEUKIN-6 PADA SEPSIS DENGAN GEJALA SYSTEMIC INFLAMMATORY RESPONSE SYNDROME (SIRS) Indranila KS; Tjahjati DM; Emma Emma
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 19, No 2 (2013)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v19i2.1064

Abstract

Sepsis is the cause of patient’s death in the intensive care unit. Sepsis is an inflammatory response to infections. Infection whencontinued can cause systemic inflammatory response syndrome (SIRS). Clinical signs and pathology of SIRS are similar to sepsis, thusclinical as well as laboratory examination is needed to distinguish these. Culture result usually can be seen at least 24 hours aftersampling. In this case a test is needed to diagnose sepsis quickly so that the patient does not experience more severe conditions. Theindicator used in the diagnostic procedure is to know the diagnostic value of procalcitonin (PCT) levels as well as of interleukin-6 (IL-6)for the diagnosis of sepsis. Thirty-two blood samples of patients treated in the Department of SIRS. Dr. Kariadi Semarang were takenconsecutively from November 2011 up to January 2012. PCT levels are determined by ELFA, levels of IL-6 were determined by ELISAmethod. To determine the sensitivity, specificity, positive predictive value and negative predictive value, 2 × 2 table was used. The ROCcurve (receiver operating characteristic) showed an area under the curve of PCT of 0.83 (95% CI = 0.66 to 0.99), cut-off 5.1 ng/mL wasused as an indicator of sepsis. PCT diagnostic test results showed 88.9% sensitivity, 73.9% specificity, 57.1% positive predictive value andnegative predictive value of 94.4%. The area under the curve for IL-6 was 0.67 (95% CI = 0.47 to 0.86), cut off 47.2 pg/mL as an indicatorof sepsis. Diagnostic tests results showed 77.8% sensitivity, 60.9% specificity, 43.7% positive predictive value and negative predictive valueof 87.5%. It can be concluded that the examination of serum PCT levels can be used as a diagnostic test (screening test) for sepsis.
RUSIP SEHUBUNGAN PROFIL LIPID DALAM KEADAAN HIPERKOLESTEROLEMIA Indranila KS; Satrianugraha MD
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 2 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i2.1074

Abstract

Rusip is a fermented fish product made from anchovies. Several lactic acid bacteria have been identified in rusip fermentation. Lacticacid bacteria are known to be able to reduce serum cholesterol. The aim of the research was to know the lipid profile changes due toadministration of rusip in hypercholesterolemia rats. The research was done by using Pre and Post Randomized Controlled Group Design.The research subjects consisted of twenty-eight Sprague Dawley strain white male rats aged 20 weeks with normal body weight, who weregiven a high-fat high-cholesterol diet, then divided into four (4) groups: control group, without Rusip administration; P1, P2 and P3 whoreceived 2.55; 5.10 and 7.65 mg Rusip/g body weight. After 14 days treatment, blood samples were taken to determine the lipid profileusing enzymatic methods. ANOVA or alternative test, was used to test the difference between the groups at the 95% confidence level. Basedon the research, the administration of Rusip in the treatment group caused significant changes in the lipid profiles of hypercholesterolemiarats (p<0.05). The total cholesterol, triglycerides and LDL cholesterol levels decreased significantly in all three treatment doses (p<0.05).The HDL cholesterol increased significantly in all three treatment doses (p<0.05). The highest change in lipid profile results was obtainedin the treatment of P3 to all variables. In this study, it was found that administration of Rusip could improve the state of blood lipidprofiles of hypercholesterolemia rats. Whereas, the highest changes were obtained in treatment with P3.
GLYCATED HEMOGLOBIN A1C AS A BIOMARKER PREDICTOR FOR DIABETES MELLITUS, CARDIOVASCULAR DISEASE AND INFLAMMATION Indranila KS
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 2 (2017)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i2.1145

Abstract

Hemoglobin glikasi (HbA1c) telah diakui secara luas sebagai petanda biologis peramal untuk keparahan Diabetes Melitus (DM).Hemoglobin glikosilasi (HbA1c) adalah petanda biologis penting yang mencerminkan kepekatan glukosa plasma puasa dan postprandialselama 120 hari sebelumnya. Telah dianggap sebagai alat penting dalam diagnosis dan manajemen diabetes. Peningkatan kadar HbA1cberarti resistensi insulin jangka panjang dan konsekuensi berat adanya hiperglikemia, dislipidemia, hiperkoagulabilitas dan responsinflamasi. Terdapat hubungan positif antara HbA1c tinggi dan hasil yang buruk pada DM, penyakit kardiovaskular (CVD) dan inflamasi.HbA1c adalah petanda biologis peramal tidak hanya di DM, tetapi juga untuk CVD dan inflamasi.
PROKALSITONIN, CRP DAN PRESEPSIN SERUM DI SIRS Hendrianingtyas Hendrianingtyas; Banundari RH; Indranila KS; Imam Budiwiyono
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 20, No 3 (2014)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v20i3.475

Abstract

Infection in ICU patients can lead to a septic condition with clinical signs similar to Systemic Inflammatory Response Syndrome (SIRS). The high risk of death and high cost of sepsis is the reason to find an early marker in diagnosing sepsis. Blood culture can givea result in 1-3 days, so C reactive protein, procalcitonin and presepsin which are fast and accurate are needed to find a septic condition in SIRS patients. The aim of this study is to determine the diagnostic value of CRP, PCT and presepsin of sepsis with blood culture as the gold standard., The samples were collected from 32 clinically SIRS patients in the Dr. Kariadi Hosiptal, Semarang. The PCT level was measured using ELFA method, CRP level by PET IA method, while presepsin level by CLEA method. The determined area was under curve (AUC) and the cut off level was determined by 2×2 table to find out the sensitivity, spesificity, positive predictive value, negative predictive value and likelihood ratio of CRP, PCT and presepsin as well. The AUC of PCT, CRP and presepsin was 0.78 (cut off 4.314 ng/mL); 0.673 (cut off 10.245 mg/L) and 0.814 (cut off 1134.5 pg/mL). The presepsin level had a higher sensitivity (90%) than PCT (80%) and CRP (70%). PCT specificity was 72.73%, presepsin and CRP specificity each was 68.18%. Based on this study, AUC and sensitivity of presepsin level were found higher than the PCT and CRP level.
MOLECULAR ASPECT CORRELATION BETWEEN GLYCATED HEMOGLOBIN (HBA1C), PROTHROMBIN TIME (PT) AND ACTIVATED PARTIAL THROMBOPLASTIN TIME (APTT) ON TYPE 2 DIABETES MELLITUS (T2DM) (Aspek molekuler Hubungan Kadar Hemoglobin Terglikasi (HbA1c), Prothrombin Time (PT) dan Activated Partial Thromboplastin Time (APTT) di Diabetes Melitus Tipe 2) Indranila KS
INDONESIAN JOURNAL OF CLINICAL PATHOLOGY AND MEDICAL LABORATORY Vol 23, No 1 (2016)
Publisher : Indonesian Association of Clinical Pathologist and Medical laboratory

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24293/ijcpml.v23i1.1175

Abstract

Diabetes Melitus (DM) memerlukan pengendalian glikemia yang dapat diketahui dengan melakukan pemeriksaan hemoglobinterglikasi (HbA1c). Semakin tinggi kadar hemoglobin terglikasi (HbA1c), semakin tidak terkendali kadar gula darah pasien DM tipe2. Hal ini dapat menyebabkan terjadinya proses hiperkoagulasi dan gangguan mikrovaskular maupun makrovaskular. PemeriksaanProtrombin Time (PT) dan Activated Partial Thromboplastin Time (APTT) diharapkan dapat mendeteksi secara dini adanya gangguankoagulasi di pasien DM tipe 2. Penelitian potong lintang terhadap 72 orang pasien DM tipe 2 yang berusia diatas 18 tahun diperiksakadar HbA1c dan dikaji koagulasi (PT dan APTT). Pasien dengan penyakit penyerta seperti anemia dan kelainan hemoglobin, keganasanatau kelainan hematologis, pasca bedah, hipertiroid, perempuan hamil, riwayat penyakit hati dan pasien yang mengkonsumsi obatobatanyang mengganggu fungsi koagulasi dikeluarkan dari penelitian ini. Uji normalitas data menggunakan Kolmogorov-Smirnovdan analisis hubungan menggunakan uji Pearson. Analisis kenasaban terdapat hubungan antara kadar hemoglobin terglikasi denganProthrombin Time negatif lemah (r= -0,179; p=0,132) dan dengan Activated Partial Thromboplastin Time positif sangat lemah (r=0,016;p=0,892). Berdasarkan telitian ini terdapat hubungan negatif lemah yang bermakna antara kadar hemoglobin terglikasi dengan PTdan hubungan positif sangat lemah yang tidak bermakna dengan Activated Partial Thomboplastin Time.
Co-Authors Banundari RH Edi Dharmana Emma Emma Hendrianingtyas Hendrianingtyas Imam Budiwiyono Pamella Mercy Papilaya Satrianugraha MD Theopilus W Watuguly Tjahjati DM