Suhartini Suhartini
Bagian Biomedik Fakultas Kedokteran Gigi Universitas Jember

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Potensi Ekstrak Teh Hijau (Camellia sinensis) Terhadap Peningkatan Jumlah Sel Fibroblas Soket Pasca Pencabutan Gigi pada Tikus Wistar (The Potency of Green Tea Extract [Camellia sinensis] Against Increase of Fibroblast Cells on Socket Post Tooth Extracti Pandika Agung Kurnia; Hengky Bowo Ardhiyanto; Suhartini .
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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In dentistry, tooth extraction is a frequent maintenance treatment. Thing to consider is healing process after extraction. Healing process consists of three phases, I.e. inflammation, fibroblastic and remodeling. Plants that are believed to accelerate wound healing is green tea. This study was aimed to look at the number of fibroblast cells after tooth extraction that was given by green tea extract (Camellia sinensis). This research was an experimental laboratory with the post-test only control group design. The samples were 24 male Wistar rats and divided into 2 groups: control group and treatment group. Each group was further divided into 3 subgroups, 3rd day, 5th day, 7th day . After extraction of left mandibular first molar tooth, rats in treatment group were given by green tea extract every day until they were decapitated according to appointed day. Tissue was histologically processed by HE staining. Count of fibroblast cells was done under a microscope on 3 field of views  with magnification 400x. Data were analyzed by using Independent T-test and it showed that on 3rd p=0,777, on 5th p=0,000, and on 7th p=0,048. Based on analysis, it could be concluded that the number of fibroblast cells in treatment group were more that in control group.   Keywords: fibroblasts, green tea extract, tooth extraction, wound healing.
Pengaruh Perbedaan Lama Waktu Distress Kronis Terhadap Perubahan Jumlah Osteoblas padaTulang Alveolar Tikus Sprague Dawley (The Effect of Differentiation Chronic Distress Duration of Osteoblast Cells Number in Alveolar Bone of Sprag Hayyu Rizky Nur Rahma; Suhartini .
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Background: Stress is a phenomenon that can occur to all organisms. Pathological stress is called distress. Distress in the long term is called chronic distress. Chronic distress will increase the secretion of cortisol, which is inducting osteoblast apoptotic and reduce osteoblast number. Purpose: Analizing the effect of differentiation chronic distress duration of osteoblast cells number in aveolar bone of Sprague dawley rats changing. Methods: Using Sprague Dawley rats with total sample of 24 animals. The rats were given an electric shock stressor (Electrical Foot Shock) as distress chronic for 7, 14, and 28 days. Stressors were given for 30 minutes at 9 a.m every day during the treatment period. The amount of electrical current is 2-8 mA, 48 V voltages and 0.5 Hz frequency. Osteoblast number were observed histologically using a microscope with 400x magnification. Results: The results showed a significant difference of 0.00 (p <0.05) between variables. Significant differences were found in the SPF 0 with SPF 4. Conclusion: Differences long time proven chronic distress affects the decrease in the number of osteoblasts in alveolar bone Sprague Dawley rats.   Keywords: Alveolar bone, chronic distress, osteoblast, Sprague daw
Perbandingan Pemberian Ikan Teri (Stolephorus Sp.) Dan Susu Kedelai Terhadap Densitas Mandibula Tikus Wistar Jantan Fadhilah R.N.; Suhartini Suhartini; Rahardyan P
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 2, No 1 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v2i1.554

Abstract

Ikan teri (Stolephorus sp.) dan susu kedelai merupakan makanan dan minuman yang mudah didapatkan. Kedua bahan tersebut menjadi sumber kalsium untuk mencegah pengurangan massa tulang. Tujuan dari penelitian ini adalah untuk mengetahui perbandingan pemberian ikan teri (Stolephorus sp.) dan susu kedelai terhadap densitas tulang mandibula. Hewan coba yang digunakan sebanyak 24 ekor tikus wistar jantan yang terbagi menjadi 3 kelompok. Kelompok I adalah kelompok kontrol, kelompok II diberi ikan teri, dan kelompok III diberi susu kedelai sebanyak 2 kali sehari. Setelah hari ke-40 hewan coba didekaputasi dan dilakukan pembedahan untuk mendapatkan tulang mandibula. Tulang mandibula di foto rontgen menggunakan foto toraks dan dilakukan pengukuran densitas tulang. Hasil pengukuran dianalisis secara statistik. Hasil uji statistik One Way ANOVA menunjukkan menunjukkan nilai probabilitas densitas tulang mandibula tikus wistar jantan sebesar 0,000 (p0,05) yang bermakna bahwa ada perbedaan signifikan terhadap densitas tulang mandibula tikus wistar jantan pada perlakuan kontrol, ikan teri dan susu kedelai lokal. Hasil dari uji Tukey HSD menunjukkan nilai probabilitas antar kelompok perlakuan 0,000 (p0,05) artinya terdapat perbedaan signifikan rerata nilai densitas tulang mandibula tikus wistar jantan. Akan tetapi, antara kelompok perlakuan ikan teri dan susu kedelai lokal menunjukkan nilai probabilitas sebesar 0,599 (p0,05) artinya tidak terdapat perbedaan signifikan dari rerata nilai densitas tulang mandibula tikus wistar jantan pada kedua kelompok perlakuan tersebut.
Identifikasi Kadar Kalsium Pada Serum Tikus Dengan Kelainan Disharmoni Oklusi Suhartini Suhartini
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 2, No 2 (2013)
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v2i2.581

Abstract

Gangguan yang terjadi pada komponen stomatognati seperti berkurangnya kontak oklusal gigi akan menyebabkan terjadinya disharmoni oklusi. Disharmoni oklusi dikenali tubuh sebagai stresor yang mempengaruhi homeostasis dalam tubuh, terutama sekresi hormon dan mineral tulang.Tujuan. Penelitian ini bertujuan untuk mengetahui kadar kalsium pada serum tikus yang mengalami kelainan disharmoni oklusi.Metode. Penelitian dilakukan pada 3 ekor tikus putih jantan Sprague dawley untuk masing-masing kelompok. Pada kelompok perlakuan dilakukan pengurangan oklusal di seluruh gigi molar sebesar 2 mm. Sedangkan untuk kelompok kontrol tidak dilakukan apa-apa. Pada hari ke-1, ke-7, ke-14 dan ke-21 dilakukan pengambilan darah melalui vena infraorbita sebanyak 2 cc kemudian disentrifugasi untuk mendapatkan serum. Pengukuran kadar kalsium dengan spektrofotometer. Hasil. Uji Anova kadar kalsium menunjukkan perbedaan yang signifikan (p0.05). Kadar kalsium yang paling tinggi terdapat pada kelompok perlakuan hari ke-21. Kesimpulan. Hasil penelitian menyimpulkan bahwa tikus yang mengalami kelainan disharmoni oklusi berpengaruh terhadap kadar kalsium pada serum.
PENINGKATAN TEKANAN DARAH PADA USIA 60-70 TAHUN DI DAERAH PANTAI PUGER KABUPATEN JEMBER Suhartini Suhartini
Jurnal Ilmu Kesehatan Masyarakat Vol 3 No 2 (2007)
Publisher : Fakultas Kesehatan Masyarakat, Universitas Jember

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At present, the problem of old-aged is increasing the proportion of old-aged, especially in Indonesia. So, the health maintenance of old-aged are important. The goal of this research is to know the increase of blood pressure in old-aged. Research typed used is abservational analytic. The data cames from blood pressure of 64 old-aged from simple random sampling  in Puger’s beach and the quisioner’s answer.The research is result of the research show that the blood pressure of old-aged is 130/81 mmHg for women old-aged and 132/81 mmHg for men old-aged. So, the conclusion of this research is increasing blood pressure of old-aged in Puger’s beach is not happen. The causes is consumption of balance food between fish, vegetables and fruits. Key words: blood pressure, old-aged, beach
EFEK PARTIKEL KEAUSAN MATERIAL PROTESA SENDI TERHADAP JUMLAH SEL RADANG (MAKROFAG) Suhartini Suhartini
STOMATOGNATIC - Jurnal Kedokteran Gigi Vol 10 No 1 (2013)
Publisher : Fakultas Kedokteran Gigi Universitas Jember

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Replacement of joints with joint prostheses is one of the treatments to restore the function of joints damage. Wear particles is considered to be one of the main factors responsible for aseptic loosening of joint prostheses. Wear particles were generated from the wear test by using pin on plate unidirectional motion machine and PBS as a lubricant. The velocity of running test is 116.5 mm/s and the force is 180 N. The sliding distance of running wear test are 15 km and 30 km. Wear particles were inserted into knee joint synovium capsule of Rattus norvegicus sp. In the control groups, knee joint synovial capsule were injected with PBS without wear particles. On seventh day, the animals were sacrified, the tissues were collected for histology examination and the number of macrophages were determined. Statistical analysis showed that on there were no significant differences (p>0.05) in the number of macrophages. It can be concluded that the wear particles from stainless steel 316 L with ion implantation and UHMWPE GUR 1120 on 15 km and 30 km of sliding distance could not affect the number of macrophages.
The Relationship between Dental Caries and Oral Hygiene of Children 7-12 Years Old at SDN Baletbaru Jember Lusi Hidayati; Dwi Warna Aju Fatmawati; Suhartini Suhartini; Agustin Wulan Suci Dharmayanti
Jurnal Kesehatan Gigi Vol 9, No 1: Juni 2022
Publisher : Jurusan Keperawatan Gigi, Poltekkes Kemenkes Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31983/jkg.v9i1.8701

Abstract

Dental caries is the most common dental and oral health problem experienced by school-age children aged 7-12 years. Dental and oral care behavior is essential in determining individual health status, especially oral health and the level of dental caries. Since the pandemic, SDN Balletbaru never provided counseling and assistance in maintaining oral health. However, since the pandemic, there has never been any assistance regarding dental and oral health maintenance. Based on this, the researchers wanted to examine the relationship between dental caries and the level of oral hygiene of children aged 7-12 years at SDN Balletbaru Jember. This descriptive research was carried out on the students of SDN Balletbaru, Jember Regency, aged 7-12 years. This research was conducted by examining OHI-s, DMF-T, and def-t. The results showed a relationship between oral hygiene, age, sex, and dental caries (R 0.5). In conclusion, there was a relationship between dental caries and the level of oral hygiene of children aged 7-12 years at SDN Balletbaru. Therefore, improving oral hygiene is necessary to promote and prevent dental caries. In order to make this effort a success, coordination and cooperation from all parties are needed, namely the students themselves, parents, teachers, and health workers
The Effect of Physical-Psychological and Psychological Stress on Blood Glucose Levels (In vivo Study) Agustin Wulan Suci Dharmayanti; Erfin Ramadana Pratama; Banun Kusumawardani; Zahreni Hamzah; Suhartini Suhartini; Tecky Indriana; Dwi Warna Aju Fatmawati
Insisiva Dental Journal: Majalah Kedokteran Gigi Insisiva Vol 12, No 1 (2023): May
Publisher : Universitas Muhammadiyah Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.18196/di.v12i1.13945

Abstract

Stress is a condition that burdens a person and affects homeostasis resulting in health problems, including dental and oral health. Stress can be caused by physical, chemical, or psychological stressors. Stressors cause an increase in blood glucose levels due to an increase in the stress hormone cortisol. This study aims to determine the effect of physical-psychological and psychological stress induced by electrical foot shock on blood glucose levels in rats. This research is an experimental laboratory study with a post-test control group design using white male rats as animal stress models. Animal models were divided into six groups, namely physical-psychological stress for 7 days (PPS1), 14 days (PPS2), and 28 days (PPS3); and psychological stress for 7 days (PS1), 14 days (PS2), and 28 days (PS3). The animal models were placed in the experimental box with electrical foot shock. The physical-psychological stress treatment was to flow an electric current of 48 V, 0.5 Hz, 2 mA for 30 minutes daily. In the psychological stress treatment, the animal models were placed without an electric shock, but the animal models were placed together, given an electric shock stressor separated by transparent plastic with a thickness of 5 cm and foam at the bottom of the cage (insulator). The results showed that although there was no effect of electrical foot shock on blood glucose levels in rats experiencing physical-psychological stress for 7, 14, and 28 exposures (p0.05), electrical foot shock affected blood glucose levels in rats with psychological stress (p0.05). It can be concluded that physical-psychological and psychological stress induced by electrical foot shock affected blood glucose levels.