Suhartini Suhartini
Bagian Biomedik Fakultas Kedokteran Gigi Universitas Jember

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Journal : e-Journal Pustaka Kesehatan

Potensi Ekstrak Teh Hijau (Camellia sinensis) Terhadap Peningkatan Jumlah Sel Fibroblas Soket Pasca Pencabutan Gigi pada Tikus Wistar (The Potency of Green Tea Extract [Camellia sinensis] Against Increase of Fibroblast Cells on Socket Post Tooth Extracti Pandika Agung Kurnia; Hengky Bowo Ardhiyanto; Suhartini .
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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In dentistry, tooth extraction is a frequent maintenance treatment. Thing to consider is healing process after extraction. Healing process consists of three phases, I.e. inflammation, fibroblastic and remodeling. Plants that are believed to accelerate wound healing is green tea. This study was aimed to look at the number of fibroblast cells after tooth extraction that was given by green tea extract (Camellia sinensis). This research was an experimental laboratory with the post-test only control group design. The samples were 24 male Wistar rats and divided into 2 groups: control group and treatment group. Each group was further divided into 3 subgroups, 3rd day, 5th day, 7th day . After extraction of left mandibular first molar tooth, rats in treatment group were given by green tea extract every day until they were decapitated according to appointed day. Tissue was histologically processed by HE staining. Count of fibroblast cells was done under a microscope on 3 field of views  with magnification 400x. Data were analyzed by using Independent T-test and it showed that on 3rd p=0,777, on 5th p=0,000, and on 7th p=0,048. Based on analysis, it could be concluded that the number of fibroblast cells in treatment group were more that in control group.   Keywords: fibroblasts, green tea extract, tooth extraction, wound healing.
Pengaruh Perbedaan Lama Waktu Distress Kronis Terhadap Perubahan Jumlah Osteoblas padaTulang Alveolar Tikus Sprague Dawley (The Effect of Differentiation Chronic Distress Duration of Osteoblast Cells Number in Alveolar Bone of Sprag Hayyu Rizky Nur Rahma; Suhartini .
Pustaka Kesehatan Vol 3 No 1 (2015)
Publisher : UPT Percetakan dan Penerbitan Universitas Jember

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Abstract

Background: Stress is a phenomenon that can occur to all organisms. Pathological stress is called distress. Distress in the long term is called chronic distress. Chronic distress will increase the secretion of cortisol, which is inducting osteoblast apoptotic and reduce osteoblast number. Purpose: Analizing the effect of differentiation chronic distress duration of osteoblast cells number in aveolar bone of Sprague dawley rats changing. Methods: Using Sprague Dawley rats with total sample of 24 animals. The rats were given an electric shock stressor (Electrical Foot Shock) as distress chronic for 7, 14, and 28 days. Stressors were given for 30 minutes at 9 a.m every day during the treatment period. The amount of electrical current is 2-8 mA, 48 V voltages and 0.5 Hz frequency. Osteoblast number were observed histologically using a microscope with 400x magnification. Results: The results showed a significant difference of 0.00 (p <0.05) between variables. Significant differences were found in the SPF 0 with SPF 4. Conclusion: Differences long time proven chronic distress affects the decrease in the number of osteoblasts in alveolar bone Sprague Dawley rats.   Keywords: Alveolar bone, chronic distress, osteoblast, Sprague daw