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IDENTIFICATION TARGETED ON toxR GENE AND DETECTION OF VIRULENT FACTOR ENCODING GENES ON Vibrio parahaemolyticus ISOLATED FROM RAW CHICKEN MEAT Fitrianda, Eka
Sistem Informasi Vol 2 No 1 (2011): Jurnal Photon
Publisher : Fakultas MIPA dan Kesehatan Universitas Muhammadiyah Riau

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (659.547 KB)

Abstract

A number of V. parahaemolyticus isolates has been isolated from raw chicken meat samples collected in Pasar raya Padang. Isolation was done using ChromagarTM Vibrio media. Identification of toxR gene was then done to these isolates. toxR is a very specific gene in V. parahaemolyticus species. Detection for the presence of genes encoding virulence factors, in this case were the gene encoding thermostable direct hemolysin (tdh) and TDH related hemolysin (trh) was performed on toxR positive V. parahaemolyticus isolates. Identification of toxR gene and detection of tdh and trh genes were done trough amplification using polymerase chain reaction PCR (method). The results showed that all of the tested V. parahaemolyticus isolates (22 isolates) had toxR gene, but none of isolates has gene encoding the production of virulence factors both tdh and trh.
Edukasi Sumber Alami Antioksidan dan Manfaatnya bagi Kesehatan di Desa Sungai Lantuang Verawati, Verawati; Fitrianda, Eka; Wahyuni, Rina
Jurnal Pengabdian Masyarakat (ABDIRA) Vol 5, No 3 (2025): Abdira, Juli
Publisher : Universitas Pahlawan Tuanku Tambusai

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31004/abdira.v5i3.696

Abstract

Antioxidants are natural or man-made substances that can prevent or delay some types of cell damage due to oxidation processes by free radicals in the body. Antioxidants are found in many foods, including fruits and vegetables. This community service aims to provide knowledge about natural sources of antioxidants and their benefits for health. This service activity was carried out for people who live in Sungai Latuang Village, Jambak Village, Batipuah Panjang Village, Padang City. The method used in this activity was counseling using the help of laptop and infocus devices. In addition to counseling, brochures and discussion sessions were also distributed as feedback to examine the participants' understanding of the material presented. Through this community service activity, there has been an increase in public understanding of the concept of antioxidants, natural sources of plants that are rich in antioxidant compounds, and their benefits for body health. This activity was attended by ± 30 people. Knowledge about natural antioxidants can add to people's insight into the importance of antioxidants in maintaining body health.