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All Journal Indonesian Journal of Pharmaceutical Science and Technology Al-Kimia Jurnal Kartika Kimia

Idar Idar

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Author-ID : 2672257

Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Computer Science & IT Education Medicine & Pharmacology

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Pembuatan Dan Aktivitas Antibakteri Yogurt Hasil Fermentasi Tiga Bakteri (Lactobacillus bulgaricus, Streptococcus thermophilus, Lactobacilus acidophilus) Dian S. Kamara; Saadah D. Rachman; Ridya Widya Pasisca; Sadiah Djajasoepana; O. Suprijana; Idar Idar; Safri Ishmayana
Al-Kimia Vol 4 No 2 (2016): December
Publisher : Study Program of Chemistry - Alauddin State Islamic University of Makassar

Exploitation of synthetic antibiotics compounds not only have positive effect for human, but also have side effect that can be unfavorable, therefore many researches are being conducted to find natural antibiotics compounds that are safer. Lactic acid bacteria has the abilitytoproduce antibacterial compound when used in fermentation process.For example, Lactobacillus acidophilus produces acidophilin and acidolin. The main purpose of the present study is to investigate antibacterial activity of yogurt fermented with mixed bacterial culture of L. bulgaricus, S. thermophilus and L. acidophilus against Escherichia coli (representing Gram negative bacteria) and Bacillus subtilis (representing Gram positive bacteria). The antibacterial activity of the yogurt at three different time points (5, 7 and 9 hours) were examined. We also investigate the fermentation parameters of the yogurt production. The results of the present study indicate that the crude yogurt extract has antibacterial activity, where the highest activity was observed at 7 hour of incubation, resulting 0.35 and 0.30 cm of clear zone against E. coli and B. subtilis, respectively. It is most likely that the compound is non protein compound.

Analisis Kandungan Fosfor (P) Dalam Dua Varietas Kubis (Brassica oleracea) Di Daerah Lembang Bandung Emma Emawati; Nesti Septi Yani; Idar Idar
Indonesian Journal of Pharmaceutical Science and Technology Supp 1, No 1 (2017)
Publisher : Indonesian Journal of Pharmaceutical Science and Technology

Fosfor merupakan nutrisi penting bagi manusia dan hewan. Selain itu, fosfor juga penting untuk pertumbuhan, pemeliharaan, perbaikan semua jaringan tubuh, dan dibutuhkan bersama dengan kalsium dan magnesium untuk pertumbuhan dan pembentukan tulang pada bayi dan anak-anak. Penelitian ini bertujuan untuk mengetahui kandungan fosfor dalam kubis putih dan ungu. Fosfor diukur dengan metode spektrofotometri Visibel, meliputi validasi analisis yang terdiri atas penetapan linieritas, batas deteksi dan batas kuantisasi, akurasi dan presisi serta analisis kadar fosfor pada sampel. Hasil penelitian menunjukan nilai linieritas 0,9991, batas deteksi dan kuantisasi dihitung secara statistik, dengan nilai 0,38 μg /ml dan 1,25 μg / ml. Presisi intra- day dan inter-day Nilaiinyatakan sebagai koe sien variansi (CV) ditentukan dalam tiga hari yang berbeda. Nilai presisi inter-day masing masing 0,67; 0.60; dan 0,80%, untuk presisi intra-day 0,82% . Perolehan kembali tingkat konsentrasi 2,0; 3,0; 4.0; dan 5,0 ug / ml kisaran 86,33% -105,01%. Kadar fosfor dalam kubis putih 20,725 mg/g, dalam kubis ungu 14,525 mg/g Dari hasil tersebut kadar fosfor dalam kubis putih ternyata lebih besar dibandingkan dengan kubis ungu tetapi kadar Fosfor tidak dipengaruhi oleh warna kubis. Kubis merupakan makanan kaya serat dan dapat dijadikan sebagai bahan makanan alternatif sebagai sumber fosfor. Kata Kunci : Fosfor (p), spektrofotometri, kubis.

Antiinflammatory Activity from Marine Microalgae Chlorella vulgaris Extract Used Human Red Blood Cells Stability Method (HRBC) Dewi kurnia; Nadya Prisdayanti; Lia Marliani; Idar Idar; Zeily Nurochman
Jurnal Kartika Kimia Vol 2 No 2 (2019): Jurnal Kartika Kimia
Publisher : Department of Chemistry, Faculty of Sciences and Informatics, Jenderal Achmad Yani University

The biologic protection response against disruption in the body can be an inflammatory reaction. Medications commonly used to treat inflammation are steroids and non-steroids which, if used over a long period, can cause adverse side effects. Several studies have shown that microalgae can be used as an anti-inflammatory agent. This research was conducted to determine the anti-inflammatory activity of the Chlorella vulgaris microalgae extract. Extraction was carried out by multilevel maceration method using n-hexane, chloroform, and ethanol. TLC monitoring results showed that the Chlorella vulgaris extract contained flavonoid, phenol, alkaloid, and steroid saponin compounds. The anti-inflammatory activity test of the extract was carried out used Human Red Blood Cell (HRBC) stability method with Na-diclophenac as a comparison standard. The results showed the inhibition value of red blood cell hemolysis (IC50) in n-hexane, chloroform and ethanol extracts respectively 150,399; 83.852 and 92.349% with the inhibition value of Na-diclophenac standard of 55.149%. Chloroform extract is known to have the most active anti-inflammatory activity which is 83,852 ppm. The results of this study indicate that the chloroform extract in Chlorella vulgaris microalgae has the potential as an anti-inflammatory agent that can be developed as an alternative anti-inflammatory drug from natural marine materials. Keywords: anti-inflammatory, Chlorella vurlgaris, Human Red Blood Cells (HRBC)

Co-Authors Dewi Kurnia Dian S. Kamara Emma Emawati, Emma Lia Marliani Nadya Prisdayanti Nesti Septi Yani O. Suprijana Ridya Widya Pasisca Saadah D. Rachman Sadiah Djajasoepana Safri Ishmayana Zeily Nurochman

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