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All Journal Jurnal Matematika Sains dan Teknologi JSFK (Jurnal Sains Farmasi & Klinis) Jurnal Ilmu Kefarmasian Indonesia
Priyo Wahyudi
Badan Pengkajian dan Penerapan Teknologi
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Mathematics Medicine & Pharmacology Public Health Other

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PRODUKSI MIKOINSEKTISIDA DARI PROPAGUL KAPANG Beauveria Bassiana Priyo Wahyudi
Jurnal Matematika Sains dan Teknologi Vol. 9 No. 2 (2008)
Publisher : LPPM Universitas Terbuka

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Abstract

Fungal insecticide or well known as mycoinsecticide is produced from propagules of entomopaghogenous fungi. It is a common knowledge that fungi can kill insects, individually or in epizootics, and methods for isolation and exploration of fungi are well known. Despite these facts, production of commercial preparations with fungi came very late. There is only one commercial product, which is produced on a large scale for several years. Beauveria bassiana is one of entomopathogenic fungus that has been used for biocontrol of many insects of crops. In this article we assess production of mycoinsecticide Beauveria bassiana through all stages of their handling, such as isolation, strain selection and optimation of production procedures both liquid and solid state fermentation. The result showed that the best way to produce mycoinsecticide in a large scale production was solid state fermentation using rice-base medium including cooked-rice and rice flour. The best incubation can be taken place in room temperature for 7 days.
Pertumbuhan Trichoderma harzianum pada Medium yang Mengandung Xilan Priyo Wahyudi; Untung Suwahyono; Sri Mulyati
JURNAL ILMU KEFARMASIAN INDONESIA Vol 2 No 1 (2004): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Xylan is one of the most important components of hemicelluloses, which could be enzimatically hydrolized by xylanolytic enzymes. One of the xylanase producing fungi is Trichoderma harzianum, which growth in a medium requires sufficient nutrient, such as Carbon, Nitrogen, microelements and vitamins. Xylan as a polysaccharide could be used as Carbon source in a growth medium of T. harzianum. The purpose of this experiment is to assess the growth ability of T. harzianum in a medium with xylan as a sole Carbon source. Three media are tested in this experiment, i.e. Medium I, Medium II, and Medium III. The result shows that the best growth of T. harzianum is obtained in Medium III, followed by Medium II and then Medium I. The best medium pH is neutral (+7) to mild acidic (6.2).
Uji Sitotoksisitas Ekstrak Etanol Herba Ceplukan (Physalis angulata Linn.) terhadap Sel T47D secara In Vitro IRA DJAJANEGARA; PRIYO WAHYUDI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 8 No 1 (2010): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Cutleaf groundcheny (Physalis angulata Linn.) is a popular traditional medicine. This experiment was conducted to investigate the possible role of the herbal extract as an anticancer agent using T47D breast cancer cell line. Cytotoxicity assay was caried out by direct counting method. Breastcancer T47D cell lines Were treated with 70% ethanol extract from cutleaf groundcherry fruit with serial dilution of 2000, 1000, 500, 250, 125, 62.5, and 31.25 µg/ml for 24 hours of incubation at 37°C As a positive control doxorubicin was also tested on the breast cancer T47D cell lines with serial dilution of 20, 10, 5, 2.5, 1.25, 0.625, 0.312, and 0.156 µg/ml for 24 hours of incubation at 37°C The amount of living cells were observed and counted, death percentage was then determined and probit analysis was used to determine the LC50 value. The LC50 value for ethanol extract from the fruits was 28,02 µg/ml While doxorubicin as the positive control was 0,113 µg/ml. It is concluded that the cutleaf groundcherry (Physalis angulata) fruits have a cytotoxic activity toward T47D (breast cancer) cell lines which confirmed previous toxicity experiment using brine shrimp lethality test (BSLT) method.
Enkapsulasi Propagul Jamur Entomopatogen Beauveria bassiana Menggunakan Alginat dan Pati Jagung sebagai Produk Mikoinsektisida PRIYO WAHYUDI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 6 No 2 (2008): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

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Abstract

Negative effects of chemical pesticides experienced by farmers have encouraged researchers to develop bioinsectiscide. Beauveria bassiana is one of entomopatogen fungus that may be used as an active compound of mycoinsecticide. The main constraint of this mycoinsecticide is retaining the viability of Beauveria bassiana propagules. The encapsulation of active propagule of Beauveria bassiana using sodium alginate and corn starch was studied in this experiment. The aim is to get the formula of mycoinsecticide with a high viable propagules contain for long storage period. Liquid fermentation were proceed to produce active biomass of Beauveria bassiana, followed by encapsulation using sodium alginate (Formula I) and corn starch (Formula II). After encapsulation and the 1st, 2nd, and 3th Week of storage in room temperature, viability test was carried out. The result showed that Formula I and II were able to immobilize effectively, but the number of viable propagule was slightly reduced. The number of viable propagule of Beauveria bassiana in Formula I and ll after three Weeks storage was 8.75 x 106 cfu/g and 6.13 x 106 cfu/g respectively. It can be concluded that encapsulation using sodium alginate and corn starch could retain propagule of Beauveria bassiana in viable number.
Penggunaan DNA Mitokondria Sebagai Penanda Sumber Gelatin Sediaan Gummy dengan Teknik Polymerase Chain Reaction dan Sekuensing DNA Andzar Fikranus Shofa; Hariyanti Hariyanti; Priyo Wahyudi
Jurnal Sains Farmasi & Klinis Vol 6, No 1 (2019): J Sains Farm Klin 6(1), April 2019
Publisher : Fakultas Farmasi Universitas Andalas

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (793.79 KB) | DOI: 10.25077/jsfk.6.1.25-31.2019

Abstract

Chewable lozenges atau gummy merupakan sediaan berbentuk kenyal yang dapat melepaskan zat aktifnya langsung di dalam mulut atau tenggorokan. Bahan yang berpengaruh dalam konsistensi gummy tersebut berasal dari basis. Dalam sediaan gummy, gelatin digunakan sebagai basis yang sebagian besar bersumber dari babi dan sapi. Identifikasi sumber bahan dapat dilakukan dengan teknik PCR dan sekuensing DNA. Penelitian ini bertujuan mengetahui sumber gelatin sediaan gummy impor tanpa logo halal. Isolasi DNA genom daging babi dan sediaan gummy dilakukan dengan GeneJet Kit. Isolat DNA kemudian diamplifikasi menggunakan primer spesifik DNA mitokondria sitokrom b. Selanjutnya, amplikon dianalisis dengan elektroforesis dan dilakukan sekuensing DNA. Hasil elektroforesis amplikon daging babi dan sediaan gummy A menghasilkan pita DNA dengan ukuran 553 bp. Analisis sekuensing DNA menunjukkan homologi dengan Sus scrofa breed long lin. Berdasarkan hasil tersebut dapat disimpulkan gelatin yang digunakan pada sediaan gummy A mengandung gelatin yang berasal dari babi Sus scrofa breed long lin.
Co-Authors Andzar Fikranus Shofa Hariyanti Hariyanti Ira Djajanegara Sri Mulyati Untung Suwahyono