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All Journal Biogenesis: Jurnal Ilmiah Biologi CERMIN: Jurnal Penelitian
Tunjung Pamekas
Jurusan Perlindungan Tanaman, Fakultas Pertanian, Universitas Bengkulu
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Economics, Econometrics & Finance Education Immunology & microbiology Social Sciences

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Induksi Umbi Mikro Kentang Secara In Vitro Pada Suhu Tinggi Dengan Beberapa Tuber Promoter Usman Kris Joko Suharjo; Bambang Gonggo Murcitro; Tunjung Pamekas; Haryuni Haryuni
Biogenesis: Jurnal Ilmiah Biologi Vol 5 No 1 (2017)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v5i1.3435

Abstract

Potato microtubers are the miniature of conventional seed tubers grown in the field. Producing potato microtuber in vitro at high temperature by applying many kinds of tuber promoting substances is similar to producing potato in the field at low elevation by applying the same tuber promoting substances. The objective of this research was to evaluate the performance of seven tuber promoters in inducing potato microtubers at high temperatures. Micro cuttings (6 weeks old) were grown at the liquid media of MS for stolon induction. Six weeks afterward, the media were moved to microtuber inducing media supplemented with tuber promoters ((FD, FD+10 COU, FD+20 COU, FD+30 COU, FD+40 COU, FD+50 COU, and IPB). The media were incubated in the dark at 20 + 2oC and 29 + 2oC. The results showed that, in general, high temperature inhibited crop growth and tuber formation. Individually, tuber promoter formula significantly affected microtuber emergence, in which IPB showed the slowest treatment in producing microtubers, the smallest number of tuber produced, and the smallest size of tubers. The interaction between temperature and tuber promoters were significantly affected the tuber formation, in which the fastest tuber formation (7,0 days ) was found in FD + 40 COU and the highest  percentage of plant producing tubers was found in FD + 30 COU. Moreover, the highest number of microtuber and the heaviest weight of microtuber were found in FD (no Coumarin). It was safely concluded that all tuber promoters were best performed at low temperature. Should anyone wish to make those tuber promoters work better at high temperature, it is recommended to increase the concentration of Coumarin added to the formula of tuber promoting substances.
KARAKTERISTIK CENDAWAN TERBAWA BENIH PADI ASAL KOTA BENGKULU Nela Zahara; Tunjung Pamekas
CERMIN: Jurnal Penelitian Vol 6 No 1 (2022): JULI
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat - Universitas Abdurachman Saleh Situbondo

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.36841/cermin_unars.v6i1.1229

Abstract

Cendawan terbawa benih padi adalah cendawan yang dibawa oleh benih padi secara internal maupun eksternal yang berpotensi untuk menyebabkan penyakit tanaman padi. Penelitian ini bertujuan untuk mendapatkan jenis cendawan patogen tebawa benih padi. Penelitian ini dilaksanakan di Laboratorium Proteksi Tanaman, Fakultas Pertanian, Universitas Bengkulu. Metode penelitian meliputi pengambilan benih padi, isolasi cendawan terbawa benih pada media PDA (Potato Dextrose Agar), dan identifikasi cendawan secara makroskopis dan mikroskopis. Penelitian ini menggunakan tiga varietas padi yang banyak digunakkan di kota Bengkulu yakni inpari 33, Inpari 36, dan Beras merah. Dari hasil penelitian diperoleh 10 isolat cendawan, jumlah dan jenis dari patogen terbawa benih padi sangat dipengaruhi oleh asal daerah benih padi dan varietas benih padi. Dari 10 isolat cendawan yang didapatkan, ada 8 cendawan yang teridentifikasi yaitu satu Curvularia sp., dua Aspergillus flavus, dua Fusarium sp, dan tiga Penicillium sp serta 2 isolat belum teridentifikasi dengan kode isolat IN33JB dan BMJD
Co-Authors Bambang Gonggo Murcitro Haryuni Haryuni Nela Zahara Usman Kris Joko Suharjo