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All Journal Biogenesis: Jurnal Ilmiah Biologi Meditory : The Journal of Medical Laboratory
Handarini Handarini
Sekolah Tinggi Ilmu Kesehatan Rajawali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science Immunology & microbiology

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Chromium(VI) Heavy Metal Biosorption in Citarum River Water Sample Using Saccharomyces cerevisiae and Rhizopus oryzae Biomass Suci Rizki Nurul Aeni; Ni'matul Murtafi'ah; Handarini Handarini
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.12274

Abstract

The Citarum River was regarded as the World’s dirtiest river in 2018, characterized by a Basin situated adjacent to several textile and electroplating industries. Hexavalent chromium metal (Cr6+) is a toxic, carcinogenic heavy metal found in the wastes of these industries. Furthermore, biosorption with biological adsorbents, including Saccharomyces cerevisiae and Rhizopus oryzae, is an alternative method for treating water polluted with heavy metals. This study therefore aims to adsorb Cr6 + heavy metals from Citarum River water using S. cerevisiae and R. oryzae biomass in two locations: Nanjung and Pangauban, with various biomass concentration and stirring speeds. For this study, 0.25, 0.5, and 0.75 g of R. oryzae suspension, as well as 250, 500, and 750 µ of S. cerevisiae were used as adsorbent, while rotated at speeds of 250 rpm, 750 rpm and 1500 rpm. Subsequently, the Cr6 + metal content was measured using a UV-Vis spectrophotometer at a wavelength of 525 nm, and calculated based on a standard curve. The results showed S. cerevisiae and R. oryzae are able to reduce the levels of Cr6+ in Citarum river water. The most reduction was obtained with the highest concentration of the adsorbents, 750 µ for S. cerevisiae and 0.75 g for R. oryzae, at the speed of 1500 rpm. S. cerevisiae and R. oryzae have great potential as biosorbents for the in situ remediation of Citarum River contaminated with heavy metals.
PENGUJIAN MEDIUM ALTERNATIF AIR CUCIAN BERAS AGAR UNTUK PERTUMBUHAN Aspergillus niger Handarini Handarini; Suyarta Efrida Pakpahan; Irma Hatimah
Meditory : The Journal of Medical Laboratory Vol 6, No 1 (2018): Meditory, volume 6 , nomer 1 , Juni 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.081 KB) | DOI: 10.33992/m.v6i1.238

Abstract

Medium pertumbuhan yang umum digunakan untuk kapang adalah Potato Dextrose Agar. Tujuan penelitian adalah menganalisa pengaruh penggunaan air cucian beras dalam beberapa konsentrasi sebagai salah satu komponen medium terhadap pertumbuhan koloni Aspergillus niger, (2) menentukan konsentrasi air cucian beras yang berpengaruh paling baik terhadap pertumbuhan koloni Aspergillus niger. Parameter pertumbuhan koloni yang diamati adalah diameter koloni dan diameter conidial head. Taraf konsentrasi air cucian beras yang diujikan adalah 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, dan 100% yang dilarutkan bersama dengan agar dan dextrose. Hasil pengujian menunjukkan adanya pengaruh penggunaan air cucian beras terhadap pertumbuhan Aspergillus niger. Koloni Aspergillus niger yang tumbuh pada medium air cucian beras dengan konsentrasi 50% sampai dengan 100% menunjukkan diameter koloni lebih besar daripada diameter koloni yang tumbuh pada medium PDA. Ukuran diameter conidial head dari sel yang tumbuh pada konsentrasi 80% sampai dengan 100% menunjukkan lebih besar dari ukuran diameter conidial head dari sel yang tumbuh pada medium PDA Konsentrasi air cucian beras yang menunjukkan pengaruh paling baik terhadap pertumbuhan koloni Aspergillus niger adalah konsentrasi 100%.
Co-Authors Irma Hatimah Ni'matul Murtafi'ah Suci Rizki Nurul Aeni Suyarta Efrida Pakpahan