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All Journal Meditory : The Journal of Medical Laboratory Jurnal Analis Kesehatan Stannum : Jurnal Sains dan Terapan Kimia Jurnal Kesehatan Rajawali
Suyarta Efrida Pakpahan
Institut Kesehatan Rajawali
Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Veterinary

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PENGUJIAN MEDIUM ALTERNATIF AIR CUCIAN BERAS AGAR UNTUK PERTUMBUHAN Aspergillus niger Handarini Handarini; Suyarta Efrida Pakpahan; Irma Hatimah
Meditory : The Journal of Medical Laboratory Vol 6, No 1 (2018): Meditory, volume 6 , nomer 1 , Juni 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.081 KB) | DOI: 10.33992/m.v6i1.238

Abstract

Medium pertumbuhan yang umum digunakan untuk kapang adalah Potato Dextrose Agar. Tujuan penelitian adalah menganalisa pengaruh penggunaan air cucian beras dalam beberapa konsentrasi sebagai salah satu komponen medium terhadap pertumbuhan koloni Aspergillus niger, (2) menentukan konsentrasi air cucian beras yang berpengaruh paling baik terhadap pertumbuhan koloni Aspergillus niger. Parameter pertumbuhan koloni yang diamati adalah diameter koloni dan diameter conidial head. Taraf konsentrasi air cucian beras yang diujikan adalah 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, dan 100% yang dilarutkan bersama dengan agar dan dextrose. Hasil pengujian menunjukkan adanya pengaruh penggunaan air cucian beras terhadap pertumbuhan Aspergillus niger. Koloni Aspergillus niger yang tumbuh pada medium air cucian beras dengan konsentrasi 50% sampai dengan 100% menunjukkan diameter koloni lebih besar daripada diameter koloni yang tumbuh pada medium PDA. Ukuran diameter conidial head dari sel yang tumbuh pada konsentrasi 80% sampai dengan 100% menunjukkan lebih besar dari ukuran diameter conidial head dari sel yang tumbuh pada medium PDA Konsentrasi air cucian beras yang menunjukkan pengaruh paling baik terhadap pertumbuhan koloni Aspergillus niger adalah konsentrasi 100%.
IDENTIFICATION Aspergillus Sp ON MUNG BEANS AT ANDIR MARKET, BANDUNG CITY Ni'matul Murtafi'ah; Suyarta Efrida Pakpahan; Muhammad Dihan
Jurnal Kesehatan Rajawali Vol 12 No 1 (2022): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.437 KB) | DOI: 10.54350/jkr.v12i1.132

Abstract

Mung bean is a carbohydrate food with a fairly high nutritional content, carbohydrate food has the composition needed by a microorganism, one of which is mushrooms. Mung bean is also a food ingredient that is often found in traditional markets such as Andir Market. The humid market environment and open storage areas make it easier for mung beansto be contaminated by fungi such asAspergillusSp. This study aims to determine the types of Aspergillus Sp which contaminates mung beanssold at Andir Market, Bandung City. The research method used is descriptive by using the total population. This research was conducted using the sowing method and observed macroscopically and microscopically. The results in this study are known to Aspergillus Sp with a percentage that isAspergillus fumigatus(40%). Aspergillus niger and Aspergillus terreus (20%) and there is 1 negative sample Aspergillus Sp. In this study it was concluded that there are types ofAspergillusSp in mung beanssold at the Andir Market, Bandung City, the types are: Aspergillus fumigatus, Aspergillus niger and Aspergillus terreus.
Ekstrak Daun Meniran (Phyllanthus niruri L.) : Bagaimana Pengaruhnya Terhadap Jumlah Trombosit di Dalam Darah? Risma Laila Sumiati; Farhan Baehaki; Nurdin Nurdin; Suyarta Efrida Pakpahan
Stannum : Jurnal Sains dan Terapan Kimia Vol 6 No 1 (2024): April 2024
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v6i1.4401

Abstract

Thrombocytopenia is a condition in which the platelet count is below normal (<150,000-450,000 cells/mm3). Until now, the treatment of thrombocytopenia has not yet found a specific drug and there are side effects from chemical treatment, so alternative solutions are needed, one of which is the use of meniran leaves (Phyllanthus niruri L.). The meniran plant is a wild plant that is often found in the wild, on the streets, as well as vacant land so that people can easily get these plants. The aim of this research was to determine the effect of meniran leaf extract (Phyllanthus niruri L.) on the number of platelets in mice induced by sodium phenytoin. The method used in this research was True Experimental with Pretest and Posttest with Control Group Design consisting of 5 groups which aimed to determine the effect of meniran leaf extract on platelet counts. The data collection technique used in this study used primary data, which was obtained from the results of examining platelet counts on day 0, day 3 after induction and day 7 after treatment with meniran leaf extract (Phyllanthus niruri L.). Then the data obtained was tested using SPSS with the non-parametric Kruskal-Wallis and Mann-Whitney tests. From 25 experimental animals, the results showed that there was a relatively significant increase in the number of platelets after being given therapy with meniran leaf extract (Phyllanthus niruri L.) but there was no difference between each dose. Based on the research results, it can be concluded that meniran leaf extract (Phyllanthus niruri L.) has an effect on increasing the number of platelets.
Jumlah Sel PMN, MN, Fibroblas, Dan Angiogenesis Pada Proses Wound healing Mencit Yang Diinduksi Alloxan Dengan Treatment Hydrogel hAM Komersial Suyarta Efrida Pakpahan; Lutfia, Salma
Jurnal Analis Kesehatan Vol. 13 No. 2 (2024): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v13i2.4707

Abstract

Luka kronis pada penderita Diabetes Mellitus (DM) dapat menghambat proses penyembuhan luka. Salah satu wound dressing yang digunakan yaitu dengan pemberian hydrogel yang bersifat fleksibilitas dan biokompatibilitas agar mampu mencapai lapisan luka yang paling dalam. Hydrogel Human Amnion Membran (hAM) komersial mengandung extracelluler matrix serta memiliki sifat anti-inflamasi dan anti- mikroba. Tujuan penelitian untuk menganalisis jumlah sel PMN, MN, fibroblas, dan angiogenesis pada proses penyembuhan luka mencit DM dengan treatment hydrogel hAM komersial. Desain penelitian ini menggunakan metode eksperimental. Mencit dibagi 3 kelompok yaitu sehat tanpa treatment, DM tanpa treatment, dan DM treatment hAM komersial. Pada hari ke 3, 7, 14, dan 21 dilakukan pengukuran luas luka (makroskopis), prosessing histologi (pewarnaan Hematoxylin Eosin), menghitung jumlah sel (mikroskopis), dan dilakukan analisis uji Two-way ANOVA (Graphpad Prism 10). Hasil penelitian menunjukkan bahwa kelompok DM hAM komersial mengalami penutupan luka lebih cepat dibandingkan kelompok sehat dan DM tanpa treatment karena jumlah sel PMN dan MN meningkat pada fase inflamasi, sel fibroblas meningkat pada fase proliferasi, dan angiogenesis meningkat pada fase remodelling dengan ditandai nilai signifikan <0.001. Kesimpulan penelitian menunjukkan hydrogel hAM komersial dapat mempercepat proses penyembuhan luka dan memberikan hasil yang signifikan terhadap jumlah sel PMN, MN, fibroblas, dan angiogenesis.
Co-Authors Farhan Baehaki Handarini Handarini Irma Hatimah Lutfia, Salma Muhammad Dihan Ni’matul Murtafi’ah Nurdin Nurdin Risma Laila Sumiati