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All Journal Meditory : The Journal of Medical Laboratory Jurnal Analis Kesehatan INDONESIAN JOURNAL OF MEDICAL LABORATORY SCIENCE AND TECHNOLOGY JURNAL ANALIS LABORATORIUM MEDIK Stannum : Jurnal Sains dan Terapan Kimia Jurnal Health Sains Jurnal Kesehatan Rajawali Jurnal Sains dan Kesehatan
Suyarta Efrida Pakpahan
Institut Kesehatan Rajawali
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Dentistry Environmental Science Health Professions Immunology & microbiology Medicine & Pharmacology Nursing Public Health Social Sciences Veterinary

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PENGUJIAN MEDIUM ALTERNATIF AIR CUCIAN BERAS AGAR UNTUK PERTUMBUHAN Aspergillus niger Handarini Handarini; Suyarta Efrida Pakpahan; Irma Hatimah
Meditory : The Journal of Medical Laboratory Vol 6, No 1 (2018): Meditory, volume 6 , nomer 1 , Juni 2018
Publisher : Jurusan Analisis Kesehatan, Poltekkes Kemenkes Denpasar

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (354.081 KB) | DOI: 10.33992/m.v6i1.238

Abstract

Medium pertumbuhan yang umum digunakan untuk kapang adalah Potato Dextrose Agar. Tujuan penelitian adalah menganalisa pengaruh penggunaan air cucian beras dalam beberapa konsentrasi sebagai salah satu komponen medium terhadap pertumbuhan koloni Aspergillus niger, (2) menentukan konsentrasi air cucian beras yang berpengaruh paling baik terhadap pertumbuhan koloni Aspergillus niger. Parameter pertumbuhan koloni yang diamati adalah diameter koloni dan diameter conidial head. Taraf konsentrasi air cucian beras yang diujikan adalah 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, dan 100% yang dilarutkan bersama dengan agar dan dextrose. Hasil pengujian menunjukkan adanya pengaruh penggunaan air cucian beras terhadap pertumbuhan Aspergillus niger. Koloni Aspergillus niger yang tumbuh pada medium air cucian beras dengan konsentrasi 50% sampai dengan 100% menunjukkan diameter koloni lebih besar daripada diameter koloni yang tumbuh pada medium PDA. Ukuran diameter conidial head dari sel yang tumbuh pada konsentrasi 80% sampai dengan 100% menunjukkan lebih besar dari ukuran diameter conidial head dari sel yang tumbuh pada medium PDA Konsentrasi air cucian beras yang menunjukkan pengaruh paling baik terhadap pertumbuhan koloni Aspergillus niger adalah konsentrasi 100%.
IDENTIFICATION Aspergillus Sp ON MUNG BEANS AT ANDIR MARKET, BANDUNG CITY Ni'matul Murtafi'ah; Suyarta Efrida Pakpahan; Muhammad Dihan
Jurnal Kesehatan Rajawali Vol 12 No 1 (2022): Jurnal Kesehatan Rajawali
Publisher : Sekolah Tinggi Ilmu Kesehatan Rajawali

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (325.437 KB) | DOI: 10.54350/jkr.v12i1.132

Abstract

Mung bean is a carbohydrate food with a fairly high nutritional content, carbohydrate food has the composition needed by a microorganism, one of which is mushrooms. Mung bean is also a food ingredient that is often found in traditional markets such as Andir Market. The humid market environment and open storage areas make it easier for mung beansto be contaminated by fungi such asAspergillusSp. This study aims to determine the types of Aspergillus Sp which contaminates mung beanssold at Andir Market, Bandung City. The research method used is descriptive by using the total population. This research was conducted using the sowing method and observed macroscopically and microscopically. The results in this study are known to Aspergillus Sp with a percentage that isAspergillus fumigatus(40%). Aspergillus niger and Aspergillus terreus (20%) and there is 1 negative sample Aspergillus Sp. In this study it was concluded that there are types ofAspergillusSp in mung beanssold at the Andir Market, Bandung City, the types are: Aspergillus fumigatus, Aspergillus niger and Aspergillus terreus.
IMPLEMENTASI FRAMEWORK PADA PEMERIKSAAN ANTIBODI COVID 19 DI UNIT PELAKSANA TEKNIS LABORATORIUM KESEHATAN KOTA BANDUNG Suyarta Efrida Pakpahan; Raudatul Jannah; Barianti
Jurnal Health Sains Vol. 3 No. 9 (2022): Jurnal Health Sains
Publisher : Syntax Corporation Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.46799/jhs.v3i9.586

Abstract

Examination of antibodies to Severe Acute Respiratory Syndrome using point-of-care immunodiagnostics has a sensitivity and specificity value of less than 95%. This study aims to determine the implementation of the framework for the examination of Covid-19 antibodies in the Technical Implementation Unit of the Bandung City Health Laboratory. Research method: This study uses a descriptive approach, explanatory, cross sectional research time with experimental quantitative methods. From the implementation of the framework for the examination of Covid-19 antibodies, it is well illustrated, indicated by high validity with a value of 0.830 > 0.30 for GB reagent, 0.983 > 0.30 and high reliability with a value of 0.860 > 0.50 for GB reagent, 0.989 > 0, 50 for the four reagents used in the Technical Implementation Unit of the Bandung City Health Laboratory, namely Bio reagent, Zy reagent, Abbt reagent. The results of the Framework Implementation on the Antibody Examination of Covid 19 for 3 reagents are not good and 4 reagents can be said to have good reliability and high reagent reliability Coronavirus Disease 2019 (Covid 19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). SARS-CoV-2 is a new type of coronavirus that has never been previously identified in humans. There are at least two types of coronavirus that are known to cause diseases that can cause severe symptoms such as Middle East Respiratory Syndrome (MERS) and Severe Acute Respiratory Syndrome (SARS).
Histopathological of Mice (Mus musculus) Liver Induced by Lead (Pb) Orally Kodariah, Liah; Efrida, Pakpahan Suyarta; Aditya, Nugraha; Nurzal, Zalzabila Rena
JURNAL INDONESIA DARI ILMU LABORATORIUM MEDIS DAN TEKNOLOGI Vol 5 No 2 (2023): Combatting Bacterial and Fungal Infections: The Critical Role of Advanced Researc
Publisher : Universitas Nahdlatul Ulama Surabaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33086/ijmlst.v5i2.4295

Abstract

Lead (Pb) is a prominent heavy metal emitted by motor vehicle exhausts, factory and mining fumes. Its presence in the atmoshpere can endure for up to seven days, posing a considerable risk of contaminating surrounding food and beverages. Lead enters the body through inhalation and the skin. Lead can also enter the human body via the oral route and accumulate in the body. It causes health problems such as oxidative stress and damage human organs such as the kidneys and liver. This research aims to examine the effect of oral lead exposure on the liver histopathology of Swiss Webster strain mice (Mus musculus). Employing a non-probability sampling technique, 25 male mice were divided into 5 groups: negative control, K2, K3, K4 and K5. These mice were administered a daily oral dose of 0.5 mL and subsequently euthanized in CO2 chamber the following week for liver dissection. The findings reveal signs of hydropic degeneration characterized by cellular swelling, irregular shapes, and disrupted organelles in groups K2, K3, K4, and K5. In addition, the mean degree of liver damage was observed as 0 for the negative control, 1 for group K2, 1 for group K3, 2 for group K4, and 3 for group K5. In conclsuin, this study confirms that lead exposure can result in dentrimenal liver histopathology changes in mice.
Pengaruh Ekstrak Bonggol Nanas (Ananas comosus L.) sebagai Biolarvasida terhadap Kematian Larva Aedes aegypti: The Effect of Pineapple Extract (Ananas comosus L.) as a Biolarvacide of Larva death of Aedes aegypti Suyarta Efrida Pakpahan; Anistia Fardila; Liah Kodariah
Jurnal Sains dan Kesehatan Vol. 5 No. 5 (2023): J. Sains Kes.
Publisher : Fakultas Farmasi, Universitas Mulawarman, Samarinda, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.25026/jsk.v5i5.2106

Abstract

The main vector that causes dengue fever which is a threat to humans is the Aedes aegypti mosquito. Chemical vector control can have a negative and resistant impact on the surrounding environment. One alternative for vector control is to use natural ingredients such as pineapple tuber extract (Ananas comosus L.). The aim of this research was to determine the effect of pineapple tuber extract (Ananas comosus L.) as a biolarvicide on the death of Aedes aegypti larvae. This research method uses an experimental method to determine the effect of pineapple tuber extract (Ananas comosus L.) on the death of Aedes aegypti larvae. Sampling was taken using purposive sampling in the form of third instar larvae. Tests were carried out with 5 concentrations of pineapple tuber extract (Ananas comosus L.), namely 0.1%, 0.5%, 1%, 1.5%, 4% as well as positive and negative controls. Then proceed with further tests with 4 concentrations, namely 3.5%, 4%, 4.5%, 5% as well as positive and negative controls. The research results show that the results of the one way ANOVA test analysis have a value of p=0.00 (p<0.05) and the results of the probit analysis show an LC50value at a concentration of 4.39%. In 24 hour observations, the lowest larval mortality was at a concentration of 3.5% with a mortality of 29% and the highest mortality was at a concentration of 5% with a mortality of 62%. Based on the research results, it was concluded that pineapple tuber extract (Ananas comosus L.) has a larvicidal effect and can be an alternative larvicide. Keywords:          Dengue fever, Aedes aegypti larvae, Biolarvicide, Pineapple tuber   Abstrak Vektor utama penyebab DBD yang menjadi ancaman bagi manusia yaitu nyamuk Aedes aegypti. Pengendalian vektor secara kimiawi dapat memberikan dampak negatif dan resisten pada lingkungan sekitar, salah satu cara alternatif pengendalian vektor yang berasal dari bahan alam seperti ekstrak bonggol nanas (Ananas comosus L.). Tujuan penelitian ini untuk mengetahui pengaruh ekstrak bonggol nanas (Ananas comosus L.) sebagai biolarvasida terhadap kematian larva Aedes aegypti. Metode penelitian ini menggunakan metode eksperimen untuk mengetahui pengaruh ekstrak bonggol nanas (Ananas comosus L.) terhadap kematian larva Aedes aegypti. Sampel menggunakan purposive sampling berupa larva instar III. Pengujian dilakukan dengan 5 konsentrasi ekstrak bonggol nanas (Ananas comosus L.) yaitu 0,1%, 0,5%, 1%, 1,5%, 4% dan kontrol positif serta negatif. Lalu dilanjutkan uji lanjutan dengan 4 konsentrasi yaitu 3,5%, 4%, 4,5%, 5% dan kontrol positif serta negatif. Hasil penelitian menunjukkan  bahwa hasil analisis uji one way ANOVA dengan nilai p=0.00 (p<0.05) dan pada hasil analisis probit didapatkan nilai LC50 pada konsentrasi 4.39%. Pada pengamatan 24 jam didapatkan kematian larva terendah pada konsentrasi 3,5% dengan kematian 29% dan kematian tertinggi pada konsentrasi 5% dengan kematian 62%. Berdasarkan hasil penelitian disimpulkan bahwa ekstrak bonggol nanas (Ananas comosus L.) memberikan pengaruh larvasida dan dapat menjadi larvasida alternatif. Kata Kunci:         Demam dengue, Larva Aedes aegypti, Biolarvasida, Bonggol Nanas
Ekstrak Daun Meniran (Phyllanthus niruri L.) : Bagaimana Pengaruhnya Terhadap Jumlah Trombosit di Dalam Darah? Risma Laila Sumiati; Farhan Baehaki; Nurdin Nurdin; Suyarta Efrida Pakpahan
Stannum : Jurnal Sains dan Terapan Kimia Vol 6 No 1 (2024): April 2024
Publisher : Department of Chemistry - Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/jstk.v6i1.4401

Abstract

Thrombocytopenia is a condition in which the platelet count is below normal (<150,000-450,000 cells/mm3). Until now, the treatment of thrombocytopenia has not yet found a specific drug and there are side effects from chemical treatment, so alternative solutions are needed, one of which is the use of meniran leaves (Phyllanthus niruri L.). The meniran plant is a wild plant that is often found in the wild, on the streets, as well as vacant land so that people can easily get these plants. The aim of this research was to determine the effect of meniran leaf extract (Phyllanthus niruri L.) on the number of platelets in mice induced by sodium phenytoin. The method used in this research was True Experimental with Pretest and Posttest with Control Group Design consisting of 5 groups which aimed to determine the effect of meniran leaf extract on platelet counts. The data collection technique used in this study used primary data, which was obtained from the results of examining platelet counts on day 0, day 3 after induction and day 7 after treatment with meniran leaf extract (Phyllanthus niruri L.). Then the data obtained was tested using SPSS with the non-parametric Kruskal-Wallis and Mann-Whitney tests. From 25 experimental animals, the results showed that there was a relatively significant increase in the number of platelets after being given therapy with meniran leaf extract (Phyllanthus niruri L.) but there was no difference between each dose. Based on the research results, it can be concluded that meniran leaf extract (Phyllanthus niruri L.) has an effect on increasing the number of platelets.
Jumlah Sel PMN, MN, Fibroblas, Dan Angiogenesis Pada Proses Wound healing Mencit Yang Diinduksi Alloxan Dengan Treatment Hydrogel hAM Komersial Suyarta Efrida Pakpahan; Lutfia, Salma
Jurnal Analis Kesehatan Vol. 13 No. 2 (2024): Jurnal Analis Kesehatan
Publisher : Department of Health Analyst, Politeknik Kesehatan, Kementerian Kesehatan Tanjungkarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26630/jak.v13i2.4707

Abstract

Luka kronis pada penderita Diabetes Mellitus (DM) dapat menghambat proses penyembuhan luka. Salah satu wound dressing yang digunakan yaitu dengan pemberian hydrogel yang bersifat fleksibilitas dan biokompatibilitas agar mampu mencapai lapisan luka yang paling dalam. Hydrogel Human Amnion Membran (hAM) komersial mengandung extracelluler matrix serta memiliki sifat anti-inflamasi dan anti- mikroba. Tujuan penelitian untuk menganalisis jumlah sel PMN, MN, fibroblas, dan angiogenesis pada proses penyembuhan luka mencit DM dengan treatment hydrogel hAM komersial. Desain penelitian ini menggunakan metode eksperimental. Mencit dibagi 3 kelompok yaitu sehat tanpa treatment, DM tanpa treatment, dan DM treatment hAM komersial. Pada hari ke 3, 7, 14, dan 21 dilakukan pengukuran luas luka (makroskopis), prosessing histologi (pewarnaan Hematoxylin Eosin), menghitung jumlah sel (mikroskopis), dan dilakukan analisis uji Two-way ANOVA (Graphpad Prism 10). Hasil penelitian menunjukkan bahwa kelompok DM hAM komersial mengalami penutupan luka lebih cepat dibandingkan kelompok sehat dan DM tanpa treatment karena jumlah sel PMN dan MN meningkat pada fase inflamasi, sel fibroblas meningkat pada fase proliferasi, dan angiogenesis meningkat pada fase remodelling dengan ditandai nilai signifikan <0.001. Kesimpulan penelitian menunjukkan hydrogel hAM komersial dapat mempercepat proses penyembuhan luka dan memberikan hasil yang signifikan terhadap jumlah sel PMN, MN, fibroblas, dan angiogenesis.
Shiva JUMLAH SEL PMN, MN, FIBROBLAS, ANGIOGENESIS PADA MENCIT DM YANG DIINDUKSI ALLOXAN Rahmawaty, Shiva Nur; Efrida Pakpahan, Suyarta
Jurnal Analis Laboratorium Medik Vol 9 No 2 (2024): JURNAL ANALIS LABORATORIUM MEDIK
Publisher : UNIVERSITAS SARI MUTIARA INDONESIA

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.51544/jalm.v9i2.5356

Abstract

Luka diabetes dapat digolongkan sebagai luka kronis akibat respon inflamasi yang memanjang. Proses penyembuhan luka pada penderita DM menjadi abormal atau lebih lama karena terjadi inflamasi persisten. Untuk menganalisis proses penutupan luka DM dan menghitung jumlah sel yang terlibat dalam proses penyembuhan luka, yaitu PMN, MN, Fibroblas, dan Angiogenesis pada hari ke 3,7, 14, dan 21. Penelitian ini menggunakan desain eksperimental dengan 24 ekor mencit jantan, dibagi menjadi kelompok DM dan sehat sebagai pembanding sebanyak tiga kali pengulangan. Dilakukan pengukuran luas (makroskopis), processing histologi (pewarnaan HE), dan menghitung jumlah sel PMN, MN, Fibroblas, Angiogenesis (Mikroskopis) pada hari ke 3, 7, 14, dan 21. Analisis data dilakukan uji Two-way Anova menggunakan GraphPad Prism 10. Pengamatan makroskopis menunjukkan kelompok sehat mengalami penutupan luka lebih cepat dibanding DM p<0,001 (***). Pada pengamatan mikroskopis menunjukkan bahwa PMN MN meningkat pada fase inflamasi hingga remodelling pada kelompok DM p<0,001 (***). Penelitian menunjukkan bahwa luka makroskopis pada mencit sehat menutup lebih cepat dibandingkan luka DM. Secara mikroskopis, kondisi histopatologi pada luka sehat lebih cepat melewati fase penyembuhan luka dibanding luka DM.
Co-Authors Aditya, Nugraha Anistia Fardila Barianti Farhan Baehaki Handarini Handarini Irma Hatimah Kodariah, Liah Liah Kodariah Lutfia, Salma Muhammad Dihan Ni’matul Murtafi’ah Nurdin Nurdin Nurzal, Zalzabila Rena Rahmawaty, Shiva Nur Raudatul Jannah Risma Laila Sumiati