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All Journal Biogenesis: Jurnal Ilmiah Biologi REINWARDTIA Sains Natural: Journal of Biology and Chemistry Borneo Journal of Biology Education (BJBE) Reinwardtia
Dedy Darnaedi
Department Of Biology, Graduate School, Universitas Nasional, Jakarta
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Education Environmental Science Immunology & microbiology Other

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The Digital Herbarium: Solutions for Data Collection and Identification of Indonesian Plant Diversity Endro Setiawan; Dedy Darnaedi; Ismail Rachman; Teguh Triono; Campbell O. Webb
Biogenesis: Jurnal Ilmiah Biologi Vol 8 No 2 (2020)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v8i2.15697

Abstract

Indonesia is one of the world’s biodiversity hotspots. It is estimated to be the home of 9.5% flowering plant species, making it the seventh country with the highest biodiversity. Plant data collection is necessary to ascertain the level of plant biodiversity, as such data help in conservation efforts and long-term management. One of the methods applied is the collection of plants, with the purpose to acquire as much data about its biological resources. The collected specimen are then gathered and processed into a herbarium to be used as an information source in managing biological resources. Unfortunately, there are some difficulties related to the making and management of a herbarium. This study aims to assess the advantages and disadvantages of photo-specimens (digital herbarium) for documenting plant biodiversity in Indonesia. The methods need steps including biological recording, specimen preparing, macro-mode capturing, and last stage identification. About 2149 plants have been gathered from Borneo, Seram, Waigeo, Flores and Sulawesi which consisted of 152 family, 512 genus, and 1,832 species, with a total of 30391 pictures of plant parts. From the experiment conducted on 672 specimens, it achieved 98.8 % accuracy on the family level and 80.1 % accuracy on the genus level, while the species level reached 78.8%. The results showed that digital herbarium can be used to conduct identification and data collection of plant biodiversity. Furthermore, this method is simple, cheap and relatively easier to conduct. The output is a catalog of plant species in specific areas, which provides better understanding about plant identification and biodiversity, enhances conservation practices, and provides better long-term protection for Indonesian plant biodiversity.
THE NANTU PLATYCERIUM GRANDE (POLYPODIACEAE), A NEW GENERIC RECORD OF PLATYCERIUM IN SULAWESI, INDONESIA Dedy Darnaedi; Lynn Clayton
REINWARDTIA Vol 19, No 2 (2020): Vol.19 No.2
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/reinwardtia.v19i2.3860

Abstract

DARNAEDI, D. & CLAYTON, L. 2020. The Nantu Platycerium grande (Polypodiaceae), a new generic record of Platycerium in Sulawesi, Indonesia. Reinwardtia 19(2): 81‒85. ‒‒ Platycerium grande (Fée) Kunze was discovered for the first time in Indonesia at the Nantu Forest, Gorontalo Province, Sulawesi. In this paper we document this discovery by providing a description and illustration of this species, as well as some brief ecological notes. Furthermore this is a new generic record of Platycerium in Sulawesi, Indonesia.   
ISOLASI DAN IDENTIFIKASI BAKTERI PENGURAI SULFIDA DARI LUMPUR MANGROVE HUTAN LINDUNG ANGKE KAPUK Eni Lestari; Dedy Darnaedi; Safendrri Komara Ragamustari
Borneo Journal of Biology Education (BJBE) Vol 4, No 1 (2022): April
Publisher : Prodi Pendidikan Biologi Universitas Borneo Tarakan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35334/bjbe.v1i1.2533

Abstract

AbstrakMangrove memiliki mikroorganisme salah satunya bakteri. Lumpur mangrove memiliki kandungan hidrogen sulfida. Bakteri aerobik memetabolisme hidrogen sulfida ini menjadi senyawa sulfat. Isolasi menggunakan medium Thiosulfat Mineral Medium. Isolat yang diamati secara makroskopis. Isolat dipilih dari hasil pengamatan mikroskopik, uji katalase dan uji motilitas. Isolat diuji juga kinerja penurunan sulfida. Isolat dengan kinerja penurunan sulfida terbaik dilanjutkan untuk uji sekuensing 16S rRNA. Hasil sekuensing menunjukkan isolat dari lumpur mangrove yang memiliki kinerja penurunan sulfida terbaik dengan nilai 30,58% adalah bakteri spesies Bacillus aryabhattaiKata kunci : mangrove, sulfida,bakteri, aerobik, BacillusAbstractMangroves have a diversity of microorganisms, one  of which is bacteria. Mangrove mud contains hydrogen sulfide. Aerobic bacteria metabolize this hydrogen sulfide to sulfate compounds. Isolation used thiosulfate mineral medium. The growing isolates observed macroscopically. Selected isolates from microscopic observation, catalase test and motility test. The isolates also tested for their sulfide reduction performance. The isolates with the best sulfide reduction performance continued for 16S rRNA sequencing assay. The best sulfide reduction performance is 30,58% and the bacteria species based result sequencing is Bacillus aryabhattai Keywords: mangrove, sulfide, bacteria, aerobic, Bacillus 
Sterilization, Multiplication and In Vitro Conservation of Germplasm Taro (Colocasia esculenta [L.] Schott) in BB BIOGEN Muhamad Sabda; Dedy Darnaedi; Dodin Koswanudin
JURNAL SAINS NATURAL Vol. 12 No. 4 (2022): Sains Natural
Publisher : Universitas Nusa Bangsa

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31938/jsn.v12i4.438

Abstract

The taro plant (Colocasia esculenta) has the potential as an alternative food that can be processed into various processed food products. Efforts to save germplasm ex-situ through taro conservation in vitro can be a backup for existing collections in the field. The activity was carried out at the in vitro conservation laboratory of the genetic resource management research group, the Bogor Institute for Research and Development of Biotechnology and Agricultural Genetic Resources (BB Biogen). A total of 15 accessions were used as explant material for sterilization, explants were grown on MS media, and then at 10 DAP, the percentage of live, contaminated, and dead explants was observed. The average sterile explants in all accessions were 34.01%, contaminated explants were 65.08%, and dead explants were 0.91%. Multiplication was carried out by subculture of the two most plantlets into the propagation medium, namely: (1) control MS, (2) MS + Thidiazuron 0.25 ppm, (3) MS + Thidiazuron 0.5 ppm, (4) MS + Thidiazuron 2 ppm, (5) MS + BA 0.5 ppm, (6) MS + BA 1 ppm, and (7) MS + BA 3 ppm. On conservation media until 5 months, the best response was shown in M3 (paclo 2 ppm) and M4 (mannitol 4%).Keywords: Aksesion; Explan; Media; ShootsABSTRAKSterilisasi, Multiplikasi, dan Konservasi In Vitro Plasma Nutfah Tanaman Talas (Colocasia esculenta) di BB BIOGENTanaman talas (Colocasia esculenta) memiliki potensi sebagai pangan alternatif yang dapat diolah menjadi berbagai produk olahan pangan. Upaya penyelamatan plasma nutfah secara ex situ melalui konservasi talas melalui in vitro dapat menjadi cadangan (back up) koleksi yang ada di lapang. Kegiatan dilaksanakan di Laboratorium konservasi in vitro kelompok peneliti Pengelolaan Sumber Daya Genetik, Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian (BB Biogen) Bogor. Sebanyak 15 aksesi digunakan sebagai materi eksplan untuk di sterilisasi, eksplan ditanam pada media MS, dan kemudian pada 10 HST diamati persentase eksplan yang hidup, terkontaminasi, dan mati. Rata–rata eksplan steril pada seluruh aksesi sebanyak 34,01%, eksplan terkontaminasi 65,08%, sedangkan eskplan yang mati sebanyak 0,91%. Multiplikasi dilakukan dengan subkultur terhadap dua planlet terbanyak ke dalam media perbanyakan, yaitu : (1) MS kontrol, (2) MS + Thidiazuron 0,25 ppm, (3) MS + Thidiazuron 0,5 ppm, (4) MS + Thidiazuron 2 ppm, (5) MS + BA 0,5 ppm, (6) MS + BA 1 ppm, dan (7) MS + BA 3 ppm. Pada media konservasi until the age of 5 months, the best response was shown in M3 (paclo 2 ppm) and M4 (mannitol 4%).Kata kunci: Aksesi; Eksplan; Media; Tunas
THE NANTU PLATYCERIUM GRANDE (POLYPODIACEAE), A NEW GENERIC RECORD OF PLATYCERIUM IN SULAWESI, INDONESIA Dedy Darnaedi; Lynn Clayton
REINWARDTIA Vol 19, No 2 (2020): Vol.19 No.2
Publisher : Research Center for Biology

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.55981/reinwardtia.2020.3860

Abstract

DARNAEDI, D. & CLAYTON, L. 2020. The Nantu Platycerium grande (Polypodiaceae), a new generic record of Platycerium in Sulawesi, Indonesia. Reinwardtia 19(2): 81‒85. ‒‒ Platycerium grande (Fée) Kunze was discovered for the first time in Indonesia at the Nantu Forest, Gorontalo Province, Sulawesi. In this paper we document this discovery by providing a description and illustration of this species, as well as some brief ecological notes. Furthermore this is a new generic record of Platycerium in Sulawesi, Indonesia.   
Co-Authors Campbell O. Webb Dodin Koswanudin Endro Setiawan Eni Lestari Ismail Rachman Lynn Clayton Muhamad Sabda Safendrri Komara Ragamustari Teguh Triono