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FERMENTASI BIAK RENDAM MOLASES DENGAN Aspergillus niger UNTUK PRODUKSI ASAM SITRAT Anne Carolina; Abubakar Sidik; Iman Permana Maksum; Saadah Diana Rachman; Agus Safari; Safri Ishmayana
Chimica et Natura Acta Vol 3, No 1 (2015)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (341.699 KB) | DOI: 10.24198/cna.v3.n1.9171

Abstract

Asam sitrat terdapat melimpah di alam dan dihasilkan sebagai salah satu zat antara pada siklus asam sitrat saat karbohidrat dioksidasi menjadi karbondioksida. Asam sitrat merupakan penyebab rasa asam pada berbagai buah seperti jeruk, nanas dan pir. Karena kelarutannya yang tinggi, rasanya yang enak dan toksisitasnya yang rendah maka asam sitrat banyak digunakan dalam industri makanan, minuman dan obat-obatan. Salah satu metode yang digunakan untuk produksi asam sitrat adalah dengan metode fermentasi. Aspergilus niger merupakan salah satu mikroorganisme yang dapat digunakan pada proses produksi asam sitrat. Produksi asam sitrat pada penelitian ini dilakukan dengan tahap pemeliharaan A. niger pada media agar miring, aktivasi kultur A. niger dalam inokulum dan produksi asam sitrat dalam media fermentasi yang mengandung 20, 25, 30 dan 35% konsentrasi molase dengan metode biak rendam. Analisis yang dilakukan mencakup perubahan pH, berat kering sel, konsumsi gula pereduksi, serta konsentrasi asam sitrat yang dihasilkan. Hasil penelitian ini menunjukkan bahwa asam sitrat paling banyak diproduksi pada media yang mengandung 30% molase, yaitu diperoleh 85,8 g/L asam sitrat.
Antioxidant and Tyrosinase Inhibitor Activities of Methanol Extracts of Acacia mangium Rita K Sari; Rahmi Utami; Irmanida Batubara; Anne Carolina; Salina Febriany
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 13, No 1 (2015): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (268.652 KB) | DOI: 10.51850/jitkt.v13i1.63

Abstract

Active compounds utilization of its extractives for cosmetic products increase the value added of mangium tree. The purpose of this study was to determine the antioxidant and an inhibitor tirosinase activities of methanol extract of the various parts of mangium tree. Phytochemical properties of the best extract were also anlayzed. Extraction was conducted by soxhletation in methanol for 12 hours. The antioxidant and tyrosinase inhibitory activities of the extracts were tested in vitro to radical of 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging and inhibition to tyrosinase enzyme. The results showed that leave resulted in the highest methanol ectract followed succesively by bark, heartwood, and sapwood the value of 10.7; 4.4; 2.5; and 0.9%, respectively. The highest antioxidant activity was bark extract and followed by leave, heartwood, and sapwood extracts with EC50 values respectively of 8.3; 26.7; 66.9; and 137.9 ppm. Only bark extract which classified as an active tyrosinase inhibitor with IC50 value of 257.8 ppm in the difenolase reaction. The IC50 value of the positive control (kojic acid) was 116.7 ppm. The other extracts relatively inactive as a tyrosinase inhibitor because their IC50 values > 1000 ppm. The qualitative analysis detect the methanol extract of mangium bark as the best extract containing phenolic compounds (phenol hirokinon, flavonoids, and tannins) and alkaloids which were thought to contribute to the high antioxidant and tyrosinase inhibitor activities.Keywords: Acacia mangium, antioxidant, extracts, tyrosinase inhibitor
Larvicide Activity of Teak Wood Powder and Its Extract to Dengue Fever Mosquito Deded S Nawawi; Anne Carolina; Cahyo Werdiningsih
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 2 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (137.557 KB) | DOI: 10.51850/jitkt.v12i2.64

Abstract

Dengue fever is transmitted to human by Aedes aegypti mosquito. Tectoquinone of teakwood extractives could be developed as natural larvicide for the mosquito due to its antifungal and insecticidal activities. However, larvicidal activities of Indonesian teak wood extract have not been investigated to a greater exten. In the present works, the larvicidal activity of teak wood extract and wood powder against fourth-instar larvae of A. aegypti was examined for different concentration of 2-methylanthraquinone based. Heartwood sample of 45 year old tree from East Java was used for the experiment. Isolation of extractives was carried out with ethanol: toluene (1:1 v/v). The concentration of 2-methylanthraquinone of the extract was determined by Pyr-Gas Chromatography Mass Spectrometry (Pyr-GC-MS). Larvacidal activity was expressed as a mortality of larva and lethal concentration (LC50 and LC90). The 2-methylanthraquinone was conformed as the main compound of teak wood extract. Teak wood extract was an effective larvicide against A. aegypti larvae. The LC50 and LC90 of the extract were found at 7.99 and 11.87 μg ml-1 of 2-methylanthraquinone, respectively. Higher lethal concentration was required by wood powder, i.e. with LC50 and LC90 of 849.30 μg ml-1 and 1051.10 μg ml-1, respectively.Keywords: Aedes aegypti, extractives, larvicide, Tectona grandis, tectoquinone
Physical, Mechanical, and Durability Properties of OSB Prepared from CCB Treated Fast Growing Tree Species Strands Wahyu Hidayat; Anne Carolina; Fauzi Febrianto
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 11, No 1 (2013): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (115.668 KB) | DOI: 10.51850/jitkt.v11i1.104

Abstract

The objective of this research was to evaluate the effect of preservative treatment of strands on the properties of oriented strand board (OSB) made from Paraserianthes falcataria, Maesopsis eminii and Acacia mangium strands. Prior to OSB manufacturing, strands were treated with chromium copper boron (CCB) soaking with concentration of 2% for 6 h. Three (3) layers OSB bonded with 7% of methylene diphenyl isocyanate (MDI) resin were produced with the core layer orientation perpendicular to the face and back layers. The results indicated that soaking strand with CCB significantly decreased the water absorption (WA) and thickness swelling (TS) values of OSB after immersed in water for 24 hours. Preservative treatment significantly decreased the mechanical properties of OSB (modulus of rupture/MOR parallel, modulus of elasticity/MOE parallel and internal bond/IB). Almost all board met the requirement of CSA 0437.0 standard for grade O-1 OSB panels, except board made from P. falcataria treated with preservative which did not achieve the requirement of TS, MOE in parallel direction, and IB. Preservative treatment strongly affected the durability of OSB. Soaking strand with CCB increased the resistance of OSB against termites attack 13.0 times higher than control board.Key words: chromium copper boron, fast growing tree species, methylene diphenyl isocyanate, oriented strand board
Distribution of Water Borne Preservative on Wood Preserved Using Full Cell and Empty Cell Processes Fauzi Febrianto; Adiyantara Gumilang; Anne Carolina; Fengky S Yoresta
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 12, No 1 (2014): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (267.915 KB) | DOI: 10.51850/jitkt.v12i1.79

Abstract

This research focused on distribution of water borne preservative on woods preserved using full cell and empty cell processes. Retention, penetration, and durability of preserved woods against dry wood termite (Cryptotermes cynochephalus LIGHT) attacked were evaluated. Pine (Pinus merkusii) and sengon (Paraserianthes falcataria) woods were preserved using 5% Wolmanit CB (water soluble preservative) using full cell and empty cell processes. The results indicated that retention of preservative preserved with full cell process was higher than empty cell process. Copper penetration on sengon wood preserved using full cell process was much higher than empty cell process. Distribution of copper and chromium on wood preserved using full cell process evenly distributed through the wood both in pine and sengon woods. They were sharply decreased from outerpart to inner part of wood when preserved using empty cell process. The whole part of pine and sengon woods preserved by either full cell or empty cell processes strongly resistance against dry wood termite attacked. Quantitative analysis of active substance of preservative using atomic absorption spectroscopy (AAS) could detect the preservative in preserved wood more accurately compared using conventional method (retention and penetration tests).Key words: distribution pattern, durability, empty cell process, full cell process, water borne preservative
Karakteristik Kimia Biomassa untuk Energi (Chemical Characteristics of Biomass for Energy) Deded S Nawawi; Anne Carolina; Tasya Saskia; Deni Darmawan; Siti L Gusvina
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 16, No 1 (2018): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (613.597 KB) | DOI: 10.51850/jitkt.v16i1.441

Abstract

The chemical components of biomass were composed mainly by three elements, i.e. carbon, hydrogen, and oxygen. These elements composition may contribute to proximate properties and calorific value of biomass. Characterization of wood, wood bark, and bamboos biomass and its relation to biomass quality for energy were carried out based on their chemical components contents. The analyses of chemical components of biomass were performed according to Technical Association of Pulp and Paper Industry (TAPPI) standard and proximate analysis was carried out according to American Society for Testing and Materials (ASTM) standard. The characteristics of biomass for energy could be evaluated based on its chemical components; especially by cellulose/lignin ratio (C/L) and hemicellulose/lignin ratio (H/L). Generally, appropriate quality of lignocellulose biomass for energy tend to exhibit low C/L and H/L ratios, that is due to obtain low volatile content and high calorific value. Lignin seems to be the most responsible chemical component for high calorific value of biomass. The classification of biomass based on the chemical components ratios would be applicable for biomass with less heterogeneity in ash content and in the quite similar extractives content.
Ultrasound-Assisted Extraction of Antimalarial Compounds from the Bidara Laut (Strychnos ligustrina) Heartwood Sri Familasari; Rita Kartika Sari; Wasrin Syafii; Anne Carolina; Umi Cahyaningsih; Siti Sa'diah
Jurnal Sylva Lestari Vol. 11 No. 3 (2023): September
Publisher : Department of Forestry, Faculty of Agriculture, University of Lampung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.23960/jsl.v11i3.682

Abstract

The aqueous wood extract of bidara laut (Strychnos ligustrina) from maceration demonstrated the greatest antimalarial activity against Plasmodium falciparum strain 3D7. However, this method has several limitations, including a lengthy extraction time, which allows for microorganism contamination. The aim of this study was to quantify the yield, evaluate the antimalarial effectiveness of the P. falciparum growth in-vitro, and analyze the aqueous bidara laut heartwood extracts phytochemical composition from the two different extraction methods,  namely the method of maceration extraction for 24 hours and the ultrasound-assisted extraction (UAE) for 30 (UAE30), 45 (UAE45), and 60 (UAE60) minutes. The bidara laut heartwood extract from the UAE60 method had relatively similar yield and antimalarial activity with the bidara laut heartwood extract from the UAE45 method. However, these extracts had higher yield and antimalarial activity than the extracts from the maceration and the UAE30 methods. The results of LC-MS/MS analysis revealed that the main compounds in the UAE45 and UAE60 extracts were strychnine, quinaprilat, and 3,4-bis-cyclobut-3-ene-1,2-dione, which has potent antimalarial properties. Keywords: antimalarial activity, Plasmodium falciparum, quinaprilat, strychnine, Strychnos ligustrina