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Protozoa Parasitik pada Ikan Sidat (Anguilla spp.) Asal Danau Lindu, Sulawesi Tengah Arif Rahman Jabal; Umi Cahyaningsih; Risa Tiuria
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 2 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (931.519 KB) | DOI: 10.18343/jipi.20.2.103

Abstract

Infection protozoan parasitic on eels (Anguilla spp.) from Lindu Lake was affected weight eels and health eels. The protozoan parasitic examinations were collection of eels, inspection eels, observation, measurement, identification of parasites, and staining protozoan used giemsa stained, trematode used semichons’s acetocarmine stained and nematode used clove oil and KOH. The diversity of parasitic protozoan found such as: Myxidium sp., Myxobolus sp., Chilodonella sp., Ceratomyxa sp., Balantidium sp., Henneguya sp., and Glugea sp. The highest prevalence Myxidium sp. in eels was 77% and no protozoan dominated between other parasites. Based on chi-square test, protozoan parasitic had a different preference to organs of eels.
Ekstrak Metanol Cengkeh (Syzygium Aromaticum (L.) Merry & Perry) Varietas Tuni Buru Selatan sebagai Antimalaria Dharmawaty M Taher; Dedi Duryadi Solihin; Umi Cahyaningsih; Purwantiningsih Sugita
Acta VETERINARIA Indonesiana Vol. 6 No. 2 (2018): Juli 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.254 KB) | DOI: 10.29244/avi.6.2.38-47

Abstract

Malaria masih menjadi salah satu masalah kesehatan di dunia dan Indonesia. Cengkeh (Syzygium aromaticum (L.) Merry & Perry) adalah tanaman asli Indonesia yang berpotensi sebagai fitofarmaka. Tujuan penelitian ini adalah untuk menganalisis aktivitas antimalaria in vitro, uji toksisitas, dan uji penghambatan secara in vivo ekstrak metanol cengkeh varietas Tuni Buru Selatan. Konsentrasi uji in vitro bunga, tangkai bunga, dan daun cengkeh masing-masing adalah 0,01, 0,1, 1, 10, dan 100 μg/mL duplo. Dosis uji toksisitas bunga cengkeh dan tangkai bunga cengkeh masing-masing 625, 1250, 2500, 5000, dan 10000 mg/kg BB dan lima ulangan. Uji in vivo dengan menggunakan tiga dosis perlakuan bunga cengkeh dan tangkai bunga cengkeh masing-masing 25, 50, dan 100 mg/kg BB dan 5 ulangan. Hasil uji in vitro menunjukkan 50% Inhibition Concentration (IC50) bunga cengkeh 1,25 μg/mL; tangkai bunga cengkeh 7,89 μg/mL dan daun cengkeh 11,42 μg/mL; 50% Lethal doses (LD50) bunga cengkeh 19743,327 dan tangkai bunga cengkeh 47304,436 mg/kg BB; Persentase penghambatan rata-rata uji in vivo bunga cengkeh 94,19; 95,81; 78,28% dan tangkai bunga cengkeh 90,48; 80,43; 74,14%. Ekstrak metanol cengkeh varietas Tuni Buru Selatan berpotensi sebagai antimalaria dan tidak toksik.
Prevalence and risk factors of Cryptosporidium spp. on dairy farms in Bogor Arifin Budiman Nugraha; Umi Cahyaningsih; Etih Sudarnika
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55961

Abstract

Cryptosporidial infection is one of the most common causes of diarrhea in humans and livestock worldwide. This study was conducted to estimate the prevalence of Cryptosporidium infection and to identify potential risk factors associated with shedding of oocysts in Bogor. A total of 308 faecal samples were collected from 136 calves less than 6 months, 44 from those 6-12 months and 128 from those than 12 months. Data of factors potentially associated with the likelihood of Cryptosporidium spp. infection were recorded (i.e., enviromental status, size of herd, and herd management). Cryptosporidium spp. oocyst was identified by using modified acid fast (Ziehl Neelsen) staining technique and microscopically examined under 400x magnifition. Results showed that the prevalence of cryptosporidiosis in Bogor was 21.1% (CI 95%; 16.5%-25.6%). The highest prevalence was 29% (CI 95%; 26.8%-31.7%) in cattle aged less than 6 months. The oocysts abundance were around <5 oocysts per microscopy visual area. Data was analyzed using logistic regression models.  Statistical analysis showed that there were association between cryptosporidiosis and calves aged less than 6 months with an odds ratio (OR) of 2.7 (CI 95%; 1.5-5.2) times compared with cattle aged more than 12 months.
Tingkat Insidensi Malaria di Wilayah Pemanasan Kelambu Berinsektisida Tahan Lama dan Wilayah Kontrol (MALARIA INCIDENCE RATE OF HEAT ASSISTED REGENERATION LONG LASTING INSECTICIDAL NETS AREA AND CONTROL) Etih Sudarnika; Mirnawati Sudarwanto; Asep Saefuddin; Umi Cahyaningsih; Upik Kesumawati Hadi; Rita Kusriastuti; Jodi Vanden Eng; Daowen Zhang; William A. Hawley
Jurnal Veteriner Vol 12 No 1 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (280.081 KB)

Abstract

Long lasting insecticidal nets (LLIN) is one effective way to prevent malaria. Permethrin treatedLLIN is one type of LLIN which is recommended by WHO. Several studies have shown that these types ofLLIN requiring heat assisted regeneration after washing to enhance the biological activity of insecticidethat contained in the LLIN fibers. This study aimed to compare the incidence rates of malaria in childrenunder five years old who live in the intervention area (where the heat assisted regeneration on LLIN afterwashing was applied) and control area (where the heat assisted regeneration on LLIN after washing wasnot applied). Data of malaria cases was collected from laboratory log book at all health centers in BangkaDistrict, in the period of June June 2007 until July 2008. Data were analyzed with Poisson regressionmodels. The results showed that the incidence rate of malaria in children under five years old was notsignificantly different between the treatment and control areas.
Ekstrak Sambiloto Menurunkan Patogenesitas Ookista Eimeria Tenella Yulia Yellita; Umi Cahyaningsih; Dyah Iswantini Pradono; Wiwin Winarsih; Wasmen Manalu
Jurnal Veteriner Vol 12 No 4 (2011)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (348.817 KB)

Abstract

Eimeria tenella is one of the nine of Eimeria species, a pathogenic intraseluler protozoa causing aviancoccidiosis. Infection was initiated by the ingestion of sporulated oocysts. The aim of this study was toinvestigate the effect of E. tenella oocyst incubation in methanol extract of Andrographis paniculata beforeinfection in broiler performance. This research used 115 broiler DOC (CP 707) devided into five groups,each group consisted of 23 broilers. The infection with 1x105 oocyst were done at the 14th day old of chicken.The 1st group was placebo (KN), while the 2nd group was infected with unincubated oocyst (KP), and theother three groups i.e. : 3rd, 4th, 5th were infected with incubated oocyst in A. paniculata extract for 2, 4, and6 hours, respectively. The number of oocysts in feces were counted on day 5th to 14th post-infection, theheterophile and macrophages were counted from caecum histology preparation, by slaughtered threechickens of each of groups on the day 0,3,6.9, and 14 post infection, and accretion body weight wasmeasured by weighing chickens per week to five-week old chickens. The results of this study indicated thatthe incubation period the sporulated oocyst in the extract of A.paniculata for six hours before infection,reduced the number of oocysts production in the feces, the number of inflammatory cells (macrophages andheterophile) in the cecum, and increases body weight (gain). In conclusion A.paniculata extract decreasedthe pathogenisity of E.tenella oocyst, so the extract of A.paniculata has good potential as anticoccidia. Itis high likely that A. paniculata extract has a potential to be anticoccidia.
Molecular Docking Senyawa Jahe Merah dan Kunyit pada Dense Granules Protein-1 Toxoplasma gondii dengan Metode In Silico Fitrine Ekawasti; Siti Sa'diah; Umi Cahyaningsih; Ni Luh Putu Indi Dharmayanti; Didik Tulus Subekti
Jurnal Veteriner Vol 22 No 4 (2021)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (220.209 KB) | DOI: 10.19087/jveteriner.2021.22.4.474

Abstract

Toksoplasmosis merupakan penyakit zoonosis yang disebabkan oleh parasit Toxoplasma gondii yang berdistribusi luas di seluruh dunia. Penyakit ini, selain mengkhawatirkan manusia juga dapat berdampak pada ekonomi peternakan karena menyebabkan penurunan produksi, gangguan pertumbuhan dan fertilitas termasuk aborsi, prematur, dan lahir mati. Prevalensi toxoplasmosis di Indonesia sangat tinggi, pada manusia sekitar 40–85%, dan pada hewan berkisar antara 5–80%. Jahe merah dan kunyit merupakan tanaman zingiberaceae yang mengandung antioksidan tinggi, yang diketahui memiliki aktivitas menghambat proses inflamasi dan berpotensi sebagai anti-toxoplasma. Namun, sampai saat ini belum diketahui mekanisme kerja senyawa aktif kunyit dan jahe merah dalam berikatan dengan reseptor protein T. gondii sebagai target pada terapi toxoplasmosis. Dilakukan uji aktivitas senyawa yang terkandung dalam jahe merah dan kunyit sebagai anti-toxoplasma terhadap protein GRA1 T. gondii dengan molecular docking secara in silico. Protein GRA1 berperan penting dalam kelangsungan hidup T. gondii. Preparasi data dasar dari struktur protein GRA1 menggunakan software PyRx, preparasi dan optimasi struktur 3D ligan jahe merah dan kunyit menggunakan aplikasi web server SWISS model prediction, serta metode molecular docking dan docking ligan pada protein GRA1 menggunakan software Autodock Vina. Hasil simulasi menunjukkan bahwa senyawa pada jahe merah dan kunyit memiliki potensi sebagai anti toxoplasmosis. Senyawa potensial demethoxycurcumin pada kunyit memiliki nilai binding affinity dan interaksi ikatan yang paling kuat terhadap protein GRA1 T. gondii.
Ekstrak Sambiloto (Andrographis paniculata) Menurunkan Jumlah Skizon, Mikrogamet, Makrogamet, dan Oosista Eimeria tenella (EXTRACT OF ANDROGRAPHIS PANICULATA DECREASED SCHIZONTS, MICROGAMETES, MACROGAMETES AND OOCYSTS NUMBER OF EIMERIA TENELLA) UMI CAHYANINGSIH; RESSY RIANDCI; DYAH ISWANTINI
Jurnal Veteriner Vol 13 No 3 (2012)
Publisher : Faculty of Veterinary Medicine, Udayana University and Published in collaboration with the Indonesia Veterinarian Association

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (196.698 KB)

Abstract

The aim of this research was to observe the effect of ethanol extract of Andrographis paniculata givenin grading doses to the schizonts, microgamete, macrogamete, and oocytes counts of Eimeria tenella inchicken caecum. A total of ninety day old broiler chicks were used in the study. At two weeks old the broilerswere divided into six groups. Each group consisted of 15 broilers, the 6 groups were: (i) negative control(broilers did not receive any treatment); (ii) positive control (each animal were infected with 104 E. tenellaoocytes); (iii) medicine control (each animal were infected with 104 E. tenella oocytes and coccidiostat); (iv)A1 (each animal were infected with 104 E. tenella oocytes and paniculata extract 90 mg/kg body weight); (v)A2 (each animal were infected with 104 E. tenella oocytes and paniculata extract 180 mg/kg body weight);and (vi) A3 (each animal were infected with 104 E. tenella oocytes and paniculata extract 360 mg/kg bodyweight). At day 6, 9, 13, 16, and 22 post infection three broilers from each group were sacrificed and theirceca were collected for histopathological examination. The results showed that paniculata extract at dose90 mg/kg body weight and 180 mg/kg body weight was able to decrease the numbers of shizont, microgamete,macrogamete, and oocytes of E. tenella in the chicken caecum.
Parasitic Protozoa found in the skin, gills, and intestines of Patin Catfish (Pangasius hypophthalmus) and Common Carp (Cyprinus carpio) Veenu Kumar; Arifin Budiman Nugraha; Umi Cahyaningsih; Tara Puri Ducha Rahmani
Journal of Natural Sciences and Mathematics Research Vol 7, No 2 (2021): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2021.7.2.11493

Abstract

The parasitic protozoa found in "Ikan Patin" or a species of Catfish (Pangasius hypophthalmus) and "Ikan Mas" or Common Carp (Cyprinus carpio) are Trichodina sp., Ichthyophthirius sp. and Myxobolus sp. These protozoa have the potential to cause death in fish and economic losses to fish farmers and sellers. This study was conducted to determine the presence of parasitic protozoa on the skin, gills, and intestines of Catfish and Carp. Samples were taken from the Bursa Ikan Hias Laladon or Laladon Ornamental Fish Market in Bogor, Indonesia. Each species was sampled for as many as 30 fish. The skin, gills, and intestines were examined using the native examination method and Lugol staining. Parasitic protozoa were identified based on their morphology, namely size, and shape. The types of parasitic protozoa found on the skin, gills, and intestines of Catfish and goldfish were Trichodina sp., Ichthyophthirius sp., and Myxobolus sp. The parasites were found in skin organs in as many as 29 samples (96.7%) from Catfish and 30 samples (100%) from Carp. The Genera of Ichthyophthirius sp. were found in the gills of 2 samples (6.7%) in Catfish and 1 sample (3.3%) from positive Carp. Myxobolus sp. was found in the intestines of 11 samples (36.7%) from Catfish and two samples (6.7%) from Carp. The species of Trichodina sp. found based on morphology was Trichodina giurusi.©2021 JNSMR UIN Walisongo. All rights reserved.
Eimeria SPECIES COMPOSITION AND FACTORS INFLUENCING OOCYSTS SHEDDING IN DAIRY FARM, BANDUNG, INDONESIA Isrok Malikus Sufi; Umi Cahyaningsih; Etih Sudarnika
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 2 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (843.28 KB) | DOI: 10.11598/btb.2017.24.2.516

Abstract

Coccidiosis is one of the most widely distributed parasitic diseases of cattle throughout the world. Coccidiosis infection in ruminants was caused by Eimeria spp.  The objective of this study was to determine Eimeria species composition and various factors influencing Eimeria oocysts shedding in dairy farm.  This study was conducted with a cross-sectional study design in dairy farm in South Bandung District from July 2014 to January 2015. Samples were obtained from 400 dairy cattle (196 cattle at age < 6 months, 37 cattle at age 6 - 12 months and 167 cattle at age > 12 months). Fecal samples were collected, examined and counted for Eimeria species composition and numbers of oocysts per gram of feces (OPG) using McMaster technique. A questionnaire was completed for individual dairy cattle farmer to record information about cattle’s health and husbandry. The effect of cattle’s sex, age and type of pen flooring to OPG values were analyzed using Mann-Whitney and Kruskal-Wallis tests. The Kruskal-Wallis test was performed followed by Dunn test as a multiple comparison test. Ten species of Eimeria were identified in all infected cattle. Among the Eimeria identified species, Eimeria bovis was found to have the highest prevalence (42.5%), followed subsequently by Eimeria wyomingensis (39.1%), Eimeria bukidnonensis (32.4%), Eimeria pellita (26.3%), Eimeria auburnensis (19.6%), Eimeria zuernii (17.3%), Eimeria cylindrica (3.9%), Eimeria canadensis (3.9%), Eimeria brasiliensis (3.4%) and Eimeria alabamensis (1.1%). The numbers of oocysts shed was correlated significantly (p < 0.05) with cattle’s sex and age as well type of pen flooring which influenced the infection pressure. Younger calves aged less than 6 months shed the highest amount of Eimeria oocysts than older cattle. Many factors may cause the increasing number of OPG in fecal samples. Therefore, it is important to keep good sanitation and control of Eimeria among dairy cattle in the KPBS Pangalengan dairy farm.
Dampak Iradiasi Sinar Gamma pada Produktivitas Aedes aegypti Jantan MIRNA WATI DEWI; Susi Soviana; Umi Cahyaningsih; Ali Rahayu
Jurnal Vektor Penyakit Vol 13 No 1 (2019): Edisi Juni
Publisher : Balai Penelitian dan Pengembangan Kesehatan Donggala, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.721 KB) | DOI: 10.22435/vektorp.v13i1.962

Abstract

Abstract Dengue fever is a vector-borne disease with Aedes aegypti as the main vector. Vector controls currently depended on insecticide. Considering the negative effect of insecticide, Sterile Insect Technique (SIT) was developed. This study was conducted to evaluate the effect of gamma-ray irradiation on the productivity of male Ae. aegypti. Male pupae age less than 15 hours were irradiated with 60 Gy and 70 Gy gamma-ray. When the pupae became adult, the sterile males mated with the same age females Aedes aegypti. Observation on fecundity, hatchability, and age was carried out until the second generation. Gamma-ray irradiation with the dose of 60 Gy and 70 Gy showed different effects on fecundity, egg hatchability, the emergence of the adult, and age of Aedes aegypti compared to control. Abstrak Demam berdarah merupakan penyakit tular vektor yang sampai saat ini masih menjadi masalah kesehatan secara global. Vektor utama yang berperan pada penyebaran penyakit DBD yaitu nyamuk Aedes aegytpi. Pengendalian vektor saat ini sangat bergantung pada penggunaan insektisida. Dampak negatif penggunaan insektisida menyebabkan pengembangan pengendalian vektor yang lain diantaranya yaitu Teknik Serangga Mandul (TSM). Penelitian ini dilakukan untuk menganalisis produktivitas Ae. aegypti jantan iradiasi hingga generasi kedua. Pupa jantan umur <15 jam diradiasi sinargamma dosis 60 Gy dan 70 Gy. Setelah menjadi nyamuk jantan dewasa segeradikawinkan dengan nyamuk betina tidak iradiasi dengan umur yang sama. Pengamatan dilakukan terutama terhadap fekunditas, daya tetas, kemunculan nyamuk, dan umurnyamuk hingga generasi kedua. Iradiasi sinar gamma dosis 60 Gy dan 70 Gymenghasilkan dampak yang berbeda terhadap fekunditas, daya tetas telur, kemunculan nyamuk dan umur nyamuk bila dibandingkan dengan kontrol.
Co-Authors A.S. Satyaningtijas Agik Suprayogi Agus Winarsongko Ali Rahayu Anne Carolina Arif Rahman Jabal Arifin Budiman Nugraha Arifin Nugraha Arifin Nugraha Arini Ratnasari ASEP SAEFUDDIN Azizah, Hanifah Nur Damiana Rita Ekastuti Daowen Zhang Dedi Duryadi Solihin Denny Widaya Lukman Desem, Muhammad Ibrahim Didik T Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Dyah Ayu Kurniawati Dyah Iswantini Effionora Anwar Eko Setyo Purwanto Eko Setyo Purwanto Ekowati Handharyani Etih Sudarnika Eva Harlina Fajar Kawitan Farlin Nepho Farlin Nepho Fitri, Arni Diana Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fong, Sulinawati Hadri Latif harimurti nuradji HERA MAHESHWARI Ichwan Yuniarto Isdoni Bustaman Isrok Malikus Sufi Isrok Malikus Sufi Jodi Vanden Eng koekoeh santoso Kurniawati, Dyah Ayu Laela N. Anisah M. Jamaluddin Assidiqi M. Mirsageri Mahdi Jufri MIRNA WATI DEWI Mirnawati Sudarwanto MUHAMMAD ADIB MUSTOFA muhammad ibrahim desem Muhammad Nurridho Wahid MULYATI EFFENDI Ni Luh Putu Indi Dharmayanti Nisrina Rosyida Noor Rifai NLP Indi Dharmayanti Nugraha, Arifin Nugraha, Arifin Budiman Pudji Achmadi Purwantiningsih Sugita Putri, Maritrana Qing Wen, Jyn Soong Raden Wisnu Nurcahyo RESSY RIANDCI Rini Damayanti Risa Tiuria Rismayani Saridewi Rita Kartika Sari Rita Kusriastuti Riza Zainuddin Ahmad Satyawardana, Wirokartiko Siti Sa&#039;diah Siti Sa'diah Siti Sa'diah Siti Sa’diah Siti Sa’diah Sri Familasari Susi Soviana Taher, Dharmawaty M. Tara Puri Ducha Rahmani Tarigan, Ronald Tjahja Muhandri Upik Kesumawati Hadi Valinata, Sisca Veenu Kumar Veerasamy, Suganti Vetnizah Juniantito Wasmen Manalu Wasrin Syafii William A. Hawley Wiwin Winarsih YENI KEZIA BEKALANI Yulia Yellita Zul Azmi Zulfiqri Z