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Protozoa Parasitik pada Ikan Sidat (Anguilla spp.) Asal Danau Lindu, Sulawesi Tengah Arif Rahman Jabal; Umi Cahyaningsih; Risa Tiuria
Jurnal Ilmu Pertanian Indonesia Vol. 20 No. 2 (2015): Jurnal Ilmu Pertanian Indonesia
Publisher : Institut Pertanian Bogor

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (931.519 KB) | DOI: 10.18343/jipi.20.2.103

Abstract

Infection protozoan parasitic on eels (Anguilla spp.) from Lindu Lake was affected weight eels and health eels. The protozoan parasitic examinations were collection of eels, inspection eels, observation, measurement, identification of parasites, and staining protozoan used giemsa stained, trematode used semichons’s acetocarmine stained and nematode used clove oil and KOH. The diversity of parasitic protozoan found such as: Myxidium sp., Myxobolus sp., Chilodonella sp., Ceratomyxa sp., Balantidium sp., Henneguya sp., and Glugea sp. The highest prevalence Myxidium sp. in eels was 77% and no protozoan dominated between other parasites. Based on chi-square test, protozoan parasitic had a different preference to organs of eels.
Ekstrak Metanol Cengkeh (Syzygium Aromaticum (L.) Merry & Perry) Varietas Tuni Buru Selatan sebagai Antimalaria Dharmawaty M Taher; Dedi Duryadi Solihin; Umi Cahyaningsih; Purwantiningsih Sugita
Acta VETERINARIA Indonesiana Vol. 6 No. 2 (2018): Juli 2018
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (39.254 KB) | DOI: 10.29244/avi.6.2.38-47

Abstract

Malaria masih menjadi salah satu masalah kesehatan di dunia dan Indonesia. Cengkeh (Syzygium aromaticum (L.) Merry & Perry) adalah tanaman asli Indonesia yang berpotensi sebagai fitofarmaka. Tujuan penelitian ini adalah untuk menganalisis aktivitas antimalaria in vitro, uji toksisitas, dan uji penghambatan secara in vivo ekstrak metanol cengkeh varietas Tuni Buru Selatan. Konsentrasi uji in vitro bunga, tangkai bunga, dan daun cengkeh masing-masing adalah 0,01, 0,1, 1, 10, dan 100 μg/mL duplo. Dosis uji toksisitas bunga cengkeh dan tangkai bunga cengkeh masing-masing 625, 1250, 2500, 5000, dan 10000 mg/kg BB dan lima ulangan. Uji in vivo dengan menggunakan tiga dosis perlakuan bunga cengkeh dan tangkai bunga cengkeh masing-masing 25, 50, dan 100 mg/kg BB dan 5 ulangan. Hasil uji in vitro menunjukkan 50% Inhibition Concentration (IC50) bunga cengkeh 1,25 μg/mL; tangkai bunga cengkeh 7,89 μg/mL dan daun cengkeh 11,42 μg/mL; 50% Lethal doses (LD50) bunga cengkeh 19743,327 dan tangkai bunga cengkeh 47304,436 mg/kg BB; Persentase penghambatan rata-rata uji in vivo bunga cengkeh 94,19; 95,81; 78,28% dan tangkai bunga cengkeh 90,48; 80,43; 74,14%. Ekstrak metanol cengkeh varietas Tuni Buru Selatan berpotensi sebagai antimalaria dan tidak toksik.
Prevalence and risk factors of Cryptosporidium spp. on dairy farms in Bogor Arifin Budiman Nugraha; Umi Cahyaningsih; Etih Sudarnika
Jurnal Sain Veteriner Vol 39, No 1 (2021): April
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22146/jsv.55961

Abstract

Cryptosporidial infection is one of the most common causes of diarrhea in humans and livestock worldwide. This study was conducted to estimate the prevalence of Cryptosporidium infection and to identify potential risk factors associated with shedding of oocysts in Bogor. A total of 308 faecal samples were collected from 136 calves less than 6 months, 44 from those 6-12 months and 128 from those than 12 months. Data of factors potentially associated with the likelihood of Cryptosporidium spp. infection were recorded (i.e., enviromental status, size of herd, and herd management). Cryptosporidium spp. oocyst was identified by using modified acid fast (Ziehl Neelsen) staining technique and microscopically examined under 400x magnifition. Results showed that the prevalence of cryptosporidiosis in Bogor was 21.1% (CI 95%; 16.5%-25.6%). The highest prevalence was 29% (CI 95%; 26.8%-31.7%) in cattle aged less than 6 months. The oocysts abundance were around <5 oocysts per microscopy visual area. Data was analyzed using logistic regression models.  Statistical analysis showed that there were association between cryptosporidiosis and calves aged less than 6 months with an odds ratio (OR) of 2.7 (CI 95%; 1.5-5.2) times compared with cattle aged more than 12 months.
Parasitic Protozoa found in the skin, gills, and intestines of Patin Catfish (Pangasius hypophthalmus) and Common Carp (Cyprinus carpio) Veenu Kumar; Arifin Budiman Nugraha; Umi Cahyaningsih; Tara Puri Ducha Rahmani
Journal of Natural Sciences and Mathematics Research Vol 7, No 2 (2021): December
Publisher : Faculty of Science and Technology, Universitas Islam Negeri Walisongo Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21580/jnsmr.2021.7.2.11493

Abstract

The parasitic protozoa found in "Ikan Patin" or a species of Catfish (Pangasius hypophthalmus) and "Ikan Mas" or Common Carp (Cyprinus carpio) are Trichodina sp., Ichthyophthirius sp. and Myxobolus sp. These protozoa have the potential to cause death in fish and economic losses to fish farmers and sellers. This study was conducted to determine the presence of parasitic protozoa on the skin, gills, and intestines of Catfish and Carp. Samples were taken from the Bursa Ikan Hias Laladon or Laladon Ornamental Fish Market in Bogor, Indonesia. Each species was sampled for as many as 30 fish. The skin, gills, and intestines were examined using the native examination method and Lugol staining. Parasitic protozoa were identified based on their morphology, namely size, and shape. The types of parasitic protozoa found on the skin, gills, and intestines of Catfish and goldfish were Trichodina sp., Ichthyophthirius sp., and Myxobolus sp. The parasites were found in skin organs in as many as 29 samples (96.7%) from Catfish and 30 samples (100%) from Carp. The Genera of Ichthyophthirius sp. were found in the gills of 2 samples (6.7%) in Catfish and 1 sample (3.3%) from positive Carp. Myxobolus sp. was found in the intestines of 11 samples (36.7%) from Catfish and two samples (6.7%) from Carp. The species of Trichodina sp. found based on morphology was Trichodina giurusi.©2021 JNSMR UIN Walisongo. All rights reserved.
Dampak Iradiasi Sinar Gamma pada Produktivitas Aedes aegypti Jantan MIRNA WATI DEWI; Susi Soviana; Umi Cahyaningsih; Ali Rahayu
Jurnal Vektor Penyakit Vol 13 No 1 (2019): Edisi Juni
Publisher : Balai Penelitian dan Pengembangan Kesehatan Donggala, Badan Penelitian dan Pengembangan Kesehatan, Kementerian Kesehatan RI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (668.721 KB) | DOI: 10.22435/vektorp.v13i1.962

Abstract

Abstract Dengue fever is a vector-borne disease with Aedes aegypti as the main vector. Vector controls currently depended on insecticide. Considering the negative effect of insecticide, Sterile Insect Technique (SIT) was developed. This study was conducted to evaluate the effect of gamma-ray irradiation on the productivity of male Ae. aegypti. Male pupae age less than 15 hours were irradiated with 60 Gy and 70 Gy gamma-ray. When the pupae became adult, the sterile males mated with the same age females Aedes aegypti. Observation on fecundity, hatchability, and age was carried out until the second generation. Gamma-ray irradiation with the dose of 60 Gy and 70 Gy showed different effects on fecundity, egg hatchability, the emergence of the adult, and age of Aedes aegypti compared to control. Abstrak Demam berdarah merupakan penyakit tular vektor yang sampai saat ini masih menjadi masalah kesehatan secara global. Vektor utama yang berperan pada penyebaran penyakit DBD yaitu nyamuk Aedes aegytpi. Pengendalian vektor saat ini sangat bergantung pada penggunaan insektisida. Dampak negatif penggunaan insektisida menyebabkan pengembangan pengendalian vektor yang lain diantaranya yaitu Teknik Serangga Mandul (TSM). Penelitian ini dilakukan untuk menganalisis produktivitas Ae. aegypti jantan iradiasi hingga generasi kedua. Pupa jantan umur <15 jam diradiasi sinargamma dosis 60 Gy dan 70 Gy. Setelah menjadi nyamuk jantan dewasa segeradikawinkan dengan nyamuk betina tidak iradiasi dengan umur yang sama. Pengamatan dilakukan terutama terhadap fekunditas, daya tetas, kemunculan nyamuk, dan umurnyamuk hingga generasi kedua. Iradiasi sinar gamma dosis 60 Gy dan 70 Gymenghasilkan dampak yang berbeda terhadap fekunditas, daya tetas telur, kemunculan nyamuk dan umur nyamuk bila dibandingkan dengan kontrol.
IDENTIFICATION OF HELMINTH AND ZOONOSIS POTENTIAL IN EEL (Anguilla spp.) FROM LINDU LAKE, SIGI DISTRICT ARIF RAHMAN JABAL; UMI CAHYANINGSIH; RISA TIURIA; ARINI RATNASARI
BIOMA : JURNAL BIOLOGI MAKASSAR Vol. 5 No. 2 (2020)
Publisher : Department of Biology, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/bioma.v5i2.11183

Abstract

Parasitic helminth infection in eel (Anguilla spp.) From the Lindu Lake affects fish health and fish weight. The purpose of this study identified parasitic helminth and zoonosis potential in eels From Lake Lindu. The helminth parasitic examinations were collection of eels, observation, measurement, identification of helminth, and staining nematode and trematode used clove oil and Semichon Acetocarmine. The species of helminth found were Anisakis sp. 44%, Anguillicola sp. 2%, and digenean 23%. The potential zoonotic is Anisakis sp., among other Helminth on eels. Eel consumption must cook because there are Anisakis sp. that have the potential for zoonosis.Keywords: Identification, Helminth Parasitic, Eel
Aktivitas Antimalaria Ekstrak Kayu Bidara Laut Wasrin Syafii; Rita K. Sari; Umi Cahyaningsih; Laela N. Anisah
Jurnal Ilmu dan Teknologi Kayu Tropis Vol 14, No 1 (2016): Jurnal Ilmu dan Teknologi Kayu Tropis
Publisher : Masyarakat Peneliti Kayu Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (167.698 KB) | DOI: 10.51850/jitkt.v14i1.6

Abstract

Penyakit malaria merupakan salah satu penyakit menular yang masih menjadi masalah utama kesehatan di Indonesia. Penelitian ini bertujuan untuk menetapkan rendemen zat ekstraktif kayu bidara laut dan aktivitas antimalarianya secara in vitro  terhadap Plasmodium falciparum serta menganalisis kandungan kimia ekstrak teraktifnya  Ekstrak kayu bidara laut dihasilkan dari maserasi dengan pelarut organik berkepolaran bertingkat (n-heksana, etil asetat, dan etanol). Hasil penelitian menunjukkan bahwa kadar ekstrak tertinggi terdapat pada ekstrak etanol (4,11%), diikuti dengan ekstrak etil asetat (1,24%) dan ekstrak n-heksana (0,55%). Berdasarkan uji aktivitas antimalaria, ekstrak etanol merupakan ekstrak teraktif (IC50 3,09 µg ml-1), sedangkan ekstrak etil asetat (IC50 81,38 µg ml-1) dan ekstrak n-heksana (IC50 889,30 µg m-1) tergolong  tidak aktif.  Hasil uji fitokimia secara kualitatif menunjukkan kelompok senyawa yang terkandung di dalam serbuk kayu bidara laut adalah flavonoid, alkaloid, triterpenoid, steroid, tannin dan hidroquinon yang diduga berperan terhadap aktivitas antimalaria. Analisis GCMS mendeteksi adanya senyawa alkaloid strychnine yang diduga memiliki aktivitas antimalaria. Berdasarkan hasil tersebut, ekstrak etanol kayu bidara laut sangat berpotensi sebagai sumber obat antimalaria.
The Role of Fennel Infusion on Estrous Cycle and Follicles Development of White Rats HERA MAHESHWARI; ARYANI SISMIN SATYANINGTIJAS; EVA HARLINA; UMI CAHYANINGSIH; MULYATI EFFENDI; MUHAMMAD ADIB MUSTOFA; YENI KEZIA BEKALANI
JURNAL ILMU KEFARMASIAN INDONESIA Vol 14 No 1 (2016): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (502.513 KB)

Abstract

This study aims to describe the estrous cycle and follicles development in female rats given infusion of fennel fruit that known to have phytoestrogen compound. Twenty five of female rats were used in this research and were divided into 5 groups. Group I was negative control group (NC), given 1 mL/100g bw of distilled water, group II was positive control group (PC), given 0.0045 mg/100g bw of ethinyl estradiol, groups III, IV and V were treatment groups (TI, T2 and T3), given fennel infusion at 36.5; 73 and 146 mg/100g bw respectively. All treatments were conducted every morning for 20 days by oral route. Changes of vaginal epithelium were observed through vaginal swabs previously stained with Giemsa stain. Histopathological examination of ovarian swere examined to reveal follicles development. Results showed that fennel fruit infusion extended the duration of estrous and metestrous phases, while shortened the proestrous and diestrous phases. Eventhough the longest estrous phase was found in T3 group, there was no significant effect on the lengthening of estrous cycle. Moreover, infusion of fennel fruit had no effect on the development of ovarian follicles, except tended to increase the number of corpus luteum.
Perbandingan Ekstrak Jahe Merah (Zingiber Officinale Roscoe. Var. Rubrum), Gingerol dan Shogaol sebagai Anti-Toksoplasma terhadap Parasit Toxoplasma Gondii Secara In-Vitro Siti Sa’diah; Effionora Anwar; Mahdi Jufri; Umi Cahyaningsih
Jurnal Jamu Indonesia Vol. 4 No. 3 (2019): Jurnal Jamu Indonesia
Publisher : Tropical Biopharmaca Research Center, IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1473.786 KB) | DOI: 10.29244/jji.v4i3.160

Abstract

Metode ekstraksi dan jenis pelarut yang digunakan akan berdampak pada proses penarikan komponen aktif sehingga akan berpengaruh juga pada aktivitasnya. Jahe merah (Zingiber officinale var. Rubrum) merupakan salah satu bahan rempah yang juga berpotensi sebagai tanaman obat. Salah satu khasiat ekstrak jahe merah adalah sebagai anti toksoplasma. Pada penelitian ini telah dilakukan pembuatan ekstrak jahe merah dengan dua jenis metode yaitu maserasi dan sokhletasi masing-masing menggunakan tiga jenis pelarut (etanol 30%, etanol 70% dan etanol 96%) sehingga diperoleh enam jenis ekstrak. Kemudian masing-masing ekstrak ditentukan kadar senyawa pencirinya meliputi 6-gingerol, 8-gingerol, 10-gingerol dan 6-shogaol dengan Kromatografi Cair Kinerja Tinggi (KCKT). Selanjutnya ekstrak diuji aktivitas antitoksoplasma secara in vitro terhadap parasit Toksoplasma gondii pada fase takhizoit yang ditumbuhkan pada sel vero. Penentuan aktivitas antitoksoplasma juga dilakukan pada senyawa murni 6, 8, 10-gingerol dan 6-shogaol serta kontrol positif antibiotik spiramisin. Hasilnya menunjukkan bahwa ekstrak etanol 96% dengan metode maserasi adalah yang paling baik aktivitasnya dan 6-gingerol adalah senyawa penciri yang paling berperan sebagai anti-toksoplasma.
PREVALENSI DAN FAKTOR RISIKO KOKSIDIOSIS PADA SAPI PERAH DI KABUPATEN BANDUNG (Prevalence and Risk Factor of Coccidiosis in Dairy Cattle in Bandung District) Isrok Malikus Sufi; Umi Cahyaningsih; Etih Sudarnika
Jurnal Kedokteran Hewan Vol 10, No 2 (2016): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (134.225 KB) | DOI: 10.21157/j.ked.hewan.v10i2.5138

Abstract

This study aimed to determine the prevalence and associated risk factors of coccidiosis. Samples were obtained from 400 dairy cattle (196 calves aged 6 months, 37 calves aged 6-12 months, and 167 calves aged 12 months). Feces samples were collected, examined and counted for prevalence and number of oocyst per gram faeces (OPG) by McMaster technique. A questionnaire was design to record information about animal health and husbandry, individually. Risk factors associated with the prevalence of Eimeria in cattle were analyzed by logistic regression model. The overall prevalence and the average of OPG of Eimeria in cattle was 179 (44.75%) and 286.75, while highest prevalence of Eimeria was observed in calves aged less than 6 months. Cattle aged more than 12 months showed significantly different relationship (P0.05) to the prevalence of Eimeria infection compare to calves aged less than 6 months and aged 6-12 months. The presence of an immature immune system in younger calves resulting in their higher susceptibility to coccidiosis. Among management and animal health practices, floor type and treatment of cattle influence the prevalence of Eimeria in cattle.
Co-Authors A.S. Satyaningtijas Agik Suprayogi Ali Rahayu Anne Carolina Arif Rahman Jabal Arifin Budiman Nugraha Arini Ratnasari ASEP SAEFUDDIN Azizah, Hanifah Nur Azmi, Zul Budi Santosa Budi Santosa Damiana Rita Ekastuti Daowen Zhang Dedi Duryadi Solihin Denny Widaya Lukman Desem, Muhammad Ibrahim Didik T Subekti Didik T Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Didik Tulus Subekti Dyah Ayu Kurniawati Dyah Iswantini Effionora Anwar Eko Setyo Purwanto Ekowati Handharyani Etih Sudarnika Eva Harlina Fajar Kawitan Farlin Nepho Fitri, Arni Diana Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti Fitrine Ekawasti, Fitrine Fong, Sulinawati Hadri Latif HERA MAHESHWARI Ichwan Yuniarto Inarsih, Dwi Isdoni Bustaman Isrok Malikus Sufi Jodi Vanden Eng koekoeh santoso Kurniawati, Dyah Ayu Laela N. Anisah M. Jamaluddin Assidiqi M. Jamaluddin Assidiqi, M. Jamaluddin M. Mirsageri M. Mirsageri, M. Mahdi Jufri MIRNA WATI DEWI Mirnawati Sudarwanto MIRNAWATI SUDARWANTO MUHAMMAD ADIB MUSTOFA muhammad ibrahim desem MULYATI EFFENDI Nepho, Farlin Ni Luh Putu Indi Dharmayanti Nisrina Rosyida Noor Rifai NLP Indi Dharmayanti Nugraha, Arifin Nugraha, Arifin Budiman Nuradji, Harimurti Nurcahyo, Raden Wisnu Nurridho Wahid, Muhammad Pudji Achmadi Purwantiningsih Sugita Purwanto, Eko Setyo Putra, Eliyus Putri, Maritrana Qing Wen, Jyn Soong RESSY RIANDCI Ridi Arif Rini Damayanti Risa Tiuria Rismayani Saridewi Rismayani Saridewi, Rismayani Rita Kartika Sari Rita Kusriastuti Riza Zainuddin Ahmad Roza Sadiah, Siti Satyawardana, Wirokartiko Sa’diah , Siti Sa’diah, Siti Siti Sa&#039;diah Siti Sa'diah Siti Sa'diah Siti Sa’diah Siti Sa’diah Sri Familasari Sufi, Isrok Malikus Susi Soviana Taher, Dharmawaty M. Tara Puri Ducha Rahmani Tarigan, Ronald Tjahja Muhandri Upik Kesumawati Hadi Valinata, Sisca Veenu Kumar Veerasamy, Suganti Vetnizah Juniantito Wasmen Manalu Wasrin Syafii William A. Hawley Winarsongko, Agus Wiwin Winarsih YENI KEZIA BEKALANI Yulia Yellita Yulia Yellita Yuniarto, Ichwan Zul Azmi Zulfiqri Z Zulfiqri Z, Zulfiqri