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All Journal JOURNAL OF COASTAL DEVELOPMENT JURNAL TEKNOLOGI LINGKUNGAN Chimica et Natura Acta
Ermin Katrin Winarno
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemistry Education Environmental Science Materials Science & Nanotechnology

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Aktivitas Sitotoksik Ekstrak Etil Asetat Daun Jati Belanda (Guazuma ulmifolia Lamk.) Terhadap Sel Kanker Leukemia L1210 Susanto Susanto; Ermin Katrin Winarno
Chimica et Natura Acta Vol 8, No 1 (2020)
Publisher : Departemen Kimia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/cna.v8.n1.26254

Abstract

Kanker merupakan penyakit yang menyebabkan tingkat kematian paling tinggi di dunia. Sel kanker merupakan sel yang pertumbuhannya tidak terkontrol dan berkembang di dalam jaringan sel tubuh. Kandungan flavonoid dan sifat antioksidan yang tinggi dari daun jati belanda (Guazuma ulmifolia Lamk) dapat digunakan sebagai pengobatan terapi kanker. Penelitian ini dilakukan untuk menentukan aktivitas sitotoksik  G. ulmifolia Lamk terhadap sel leukemia L1210. Daun G. ulmifolia kering dimaserasi secara bertahap menggunakan pelarut n-heksana, etil asetat, dan etanol. Masing-masing ekstrak yang diperoleh dievaluasi aktivitas sitotoksisitasnya terhadap sel leukemia L1210. Ekstrak paling aktif difraksinasi menggunakan kromatografi kolom dengan berbagai pelarut yang sesuai. Selanjutnya semua subfraksi yang diperoleh diuji aktivitas sitotoksiknya dan dianalisis dengan kromatografi cair kinerja tinggi (KCKT) untuk mengetahui bentuk kromatogranya. Hasil penelitian menunjukkan bahwa ekstrak etil setat memiliki nilai IC50 paling kecil diantara ekstrak yang lain, yaitu sebesar 6,23 µg/mL. Fraksinasi ekstrak etil asetat diperoleh (1-8) fraksi dengan fraksi 4 memiliki aktivitas paling tinggi yang  berpotensi sebagai anti kanker dengan nilai IC50 sebesar 2,67 µg/mL. Analisis fraksi 4 etil asetat G ulmifolia menggunakan KCKT menunjukkan bahwa kandungan utama adalah flavonoid.
PENGARUH IRADIASI GAMMA TERHADAP DEGRADASI ZAT WARNA DIRECT ORANGE 34 DALAM AIR Purwantiningsih Sugita; Ermin Katrin Winarno; Lia Anriani
Jurnal Teknologi Lingkungan Vol. 1 No. 2 (2000): JURNAL TEKNOLOGI LINGKUNGAN
Publisher : Center for Environmental Technology - Agency for Assessment and Application of Technology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (622.851 KB) | DOI: 10.29122/jtl.v1i2.170

Abstract

The aim of this experiment is study on technique of gamma irradiaton in analyzing dying agent direct orange 34 in waste water. Direct orange 34 was dispersed to oxalic and malic acids when the 60Co irradiated. The result showed that dose and dose rate of 60Co were less than 10 kGy and 7.57 kGy/hour, respectively. While, the pH of the first solution was 7.0. The technique showed that the chemical oxygen demand of dye solution was decreased from 60.38 to 4.90 ppm.
HYDROCARBON DISTRIBUTION IN SEDIMENTS OF JAKARTA BAY Agustin Sumartono; Winarti Andayani; Ermin Katrin Winarno
JOURNAL OF COASTAL DEVELOPMENT Vol 11, No 1 (2007): Volume 11, Number 1, Year 2007
Publisher : JOURNAL OF COASTAL DEVELOPMENT

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (3592.986 KB)

Abstract

The analysis of sediments hom stations I, 29, 9, I5, 17 and 25 located in Jakarta Bay were carried out. Sampels were Soxhlet-extracted for I4 hours with methylene chloride J methanol (I: I ), followed by saponi/icatian using KOH in methanol and benzene, Unsaponwcation parts were extracted using n-hexanes. Extracted samples were analyzed using gas chromatography with capiler column of SPB-I. From chromatogram results it was noted that the analysis of sea sediment sea from each station in Jakarta Bay indicate the distribution if n-allranes in the range of CN-C;6 which were dominated by an even carbon. From the results it is assumed that Jakarta Bay sediment may have natural hydrocarbon sources which may come from either the red or blue algae.
Co-Authors Agustin Sumartono Lia Anriani Purwantiningsih Sugita Susanto Susanto Winarti Andayani