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Winarti Andayani
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HYDROCARBON DISTRIBUTION IN SEDIMENTS OF JAKARTA BAY Agustin Sumartono; Winarti Andayani; Ermin Katrin Winarno
JOURNAL OF COASTAL DEVELOPMENT Vol 11, No 1 (2007): Volume 11, Number 1, Year 2007
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

The analysis of sediments hom stations I, 29, 9, I5, 17 and 25 located in Jakarta Bay were carried out. Sampels were Soxhlet-extracted for I4 hours with methylene chloride J methanol (I: I ), followed by saponi/icatian using KOH in methanol and benzene, Unsaponwcation parts were extracted using n-hexanes. Extracted samples were analyzed using gas chromatography with capiler column of SPB-I. From chromatogram results it was noted that the analysis of sea sediment sea from each station in Jakarta Bay indicate the distribution if n-allranes in the range of CN-C;6 which were dominated by an even carbon. From the results it is assumed that Jakarta Bay sediment may have natural hydrocarbon sources which may come from either the red or blue algae.
SAXITOXIN IN GREEN MUSSELS (Perna viridis, Mytiliae), BLOOD COCKLE (Anadara granosa) AND FEATHERS COCKLE (Anadara antiquata, Arcidae) USING HIGH PRESSURE LIQUID CHROMATOGRAPHY Winarti Andayani; Agustin Sumartono
JOURNAL OF COASTAL DEVELOPMENT Vol 15, No 3 (2012): Volume 15, Number 3, Year 2012
Publisher : JOURNAL OF COASTAL DEVELOPMENT

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Abstract

Saxitoxin (STX) was measured in green mussels (Perna viridis), and  feathers cockle (Anadara antiquata, Arcidae) from Jakarta and blood cockle (Anadara granosa) from Jakarta and Indramayu. Samples were taken 7 times from fish market Muara Baru Jakarta and Karangsong Indramayu. All samples were collected from June up to October 2009. The aim of this research is to find out  the content of STX in the mussels. The mussels tissues  were homogenized, weighed and extracted with 0.1 M HCl. The supernatan were filtered by 0.45 μ nylon membran. Fluorescence oxidation of STX was carried out using 2% H2O2 in alkaline solution. Analysis was performed using HPLC equipped with a C18 column (4.6 mm×250 mm, 5 μm), fluorescence detection (ex 340 nm, em 400 nm) elution of acetonitrile/0.1 M ammonium formate solution (5:95, v/v, pH 6) at a flow rate of 1.0 ml/ min. The calibration graphs was prepared by injecting standards ranging from 0,5–20 ng/ml, giving an acceptable linearity (r = 0.999). Retention time of saxitoxin standard was detected at 5.467 min. Negative results were obtained for most of blood mussels from Indramayu with the exception of  the 4th and 7th sampling. Saxitoxin were detected   in Perna viridis and Anadara antiquata, Arcidae ranging from 0.87–5.39 µg/ 100 g and  0.14–0.9 µg/ 100 g wet tissues respectively.
Co-Authors Agustin Sumartono Ermin Katrin Winarno