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Efektivitas Antifungi Ekstrak Curcuma aeruginosa terhadap Patogenisitas Colletotrichum capsici pada Tanaman Cabai Merah (Antifungi Effectivity of Curcuma aeruginosa Extract to Colletotrichum capsici Pathogenicity on Red Chilli Pepper) Anella Retna Kumala Sari; Arrohmatus Syafaqoh Li’aini
Jurnal Hortikultura Vol 30, No 2 (2020): Desember 2020
Publisher : Indonesian Center for Horticulture Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jhort.v30n2.2020.p141-152

Antraknosa yang disebabkan oleh Colletotrichum capsici masih menjadi penyakit utama yang menyerang buah cabai merah. Selama ini, penggunaan Curcuma aeruginosa sebagai antimikrobe patogen penyebab penyakit pada manusia lebih populer daripada sebagai antimikrobe fitopatogen. Rimpang Curcuma diketahui mengandung senyawa volatil/atsiri dan nonvolatil. Potensi senyawa volatil/atsiri Curcuma sebagai antimikrobe telah banyak dilaporkan, namun masih sangat terbatas untuk senyawa nonvolatilnya. Penelitian ini bertujuan mengetahui efektivitas antifungi senyawa nonvolatil dari ekstrak C. aeruginosa terhadap C. capsici pada buah cabai merah. Penelitian dilaksanakan pada bulan November 2014 hingga Mei 2015 di Universitas Brawijaya. Senyawa nonvolatil didapatkan dengan merendam rimpang C. aeruginosa menggunakan pelarut metanol kemudian didistilasi menggunakan rotary vacuum evaporator dan diidentifikasi menggunakan HPLC. Efektivitas antifungi senyawa nonvolatil dari ekstrak rimpang C. aeruginosa diuji secara in vitro dan in vivo di laboratorium menggunakan Rancangan Acak Lengkap dengan tiga kali ulangan. Perlakuan yang diujikan yaitu konsentrasi senyawa nonvolatil terdiri atas 0 (kontrol), 4, 6, 8, 10, dan 12 ppm. Senyawa nonvolatil dari ekstrak rimpang C. aeruginosa sangat efektif menghambat pertumbuhan C. capsici baik secara in vivo maupun in vitro di laboratorium bahkan konsentrasi 12 ppm menunjukkan persentase penghambatan antraknosa hingga 100%. Hasil identifikasi HPLC menunjukkan bahwa senyawa nonvolatil dari ekstrak rimpang C. aeruginosa mengandung kelompok Curcuminoid yang terdiri atas curcumin dan demethoxycurcumin yang berperan sebagai antifungi sehingga sangat berpotensi dikembangkan sebagai fungisida nabati.KeywordsAntraknosa; Cabai merah; C. aeruginosa; Senyawa nonvolatilAbstractAnthracnose caused by Colletotrichum capsici is still as major disease against chilli pepper fruit. During this time, utilization of C. aeruginosa as pathogen antimicrobial caused disease to human is more popular than to crops. Curcuma has been known containing volatile and nonvolatile compound. Potential of volatil compound from Curcuma as antipathogen has been reported widely, nevertheless it is still limited known for nonvolatile compound. This research aimed to understand the antifungi effectivity of nonvolatile compound from C. aeruginosa extract to C. capsici on chilli pepper fruit. Research was conducted in November 2014 to Mei 2015. Nonvolatile compound was obtained by soaking C. aeruginosa rhizome into methanol solvent then distilated using rotary vacuum evaporator and identified with HPLC instrument. Antifungi effectivity of nonvolatile compound from C. aeruginosa extract was experimented by in vitro and in vivo test using Completely Randomized Design with three replications. Treatments tested were various concentrations of nonvolatile compound namely 0 (control), 4, 6, 8,10, and 12 ppm. Nonvolatile compound from C. aeruginosa extract was highly effective to inhibit growth of C. capsici by both in vitro and in vivo test. HPLC identification result showed nonvolatile compound from C. aeruginosa extract contains Curcuminoid group play role as antifungi.

POTENCY OF BLACK TEA FILTRATE AS UV PROTECTANT TO S/NPV JTM 97 C IN CONTROLLING HELICOVERPA ARMIGERA ON SOYBEAN Anella Retna Kumala Sari; Tiara Eka Ariestantia; Fery Abdul Choliq
Jurnal Penelitian Tanaman Industri Vol 26, No 1 (2020): June, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n1.2020.40-48

Helicoverpa armigera is the important pest on soybean which usually controlled by chemical insecticide spraying, so it should be replaced by naturally control using biological agents such as Spodoptera litura Nuclear Polyhedrosis Virus (S/NPV) JTM 97 C. It has some weakness such as during it’s applied in the field the effectiveness can become decreased after exposure the sunlight, especially ultraviolet (UV) so it needs the protective material, such as black tea filtrate which is known containing epigallocatechin gallate, caffeic acid, and apigenin which have role as UV protectors. This research aimed to investigate the potential of black tea filtrate to protect the S/NPV JTM 97 C from UV in controlling of H. armigera. The research was conducted in Pests and Diseases Laboratory on Indonesian Legumes and Tuber Crops Research Institute (ILETRI), Kendalpayak, Malang from January to June 2015. The research used Completely Randomized Design with seven treatments and four replications. They were S/NPV JTM 97 C without UV protectant as control (P1), S/NPV JTM 97 C + kaolin as comparison (P2), S/NPV JTM 97 C + black tea filtrate concentration 0.5 (P3), 1.0 (P4), 1.5 (P5), 2 (P6), and 2.5% (P7). Results showed that all of the protective materials of black tea filtrate were unable to provide mortality of H. armigera larvae better than kaolin. Kaolin can cause mortality of H. armigera larvae up to 82,50%. All of Black tea filtrate concentrations tested were not able to be used as protectant of S/NPV JTM 97 C due to it can only cause mortality of H. armigera up to 47,50% . Keywords: Entomopathogenic virus, LC50, protectant material, pest.

EFEKTIVITAS SENYAWA NONATSIRI DARI Curcuma spp. TERHADAP PENEKANAN PENYAKIT ANTRAKNOSA PADA BUAH CABAI Anella Retna Kumala Sari; Firdaus Auliya Rahmah; Syamsuddin Djauhari
Buletin Penelitian Tanaman Rempah dan Obat Vol 31, No 1 (2020): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v31n1.2020.21-30

One of the important diseases on chili is anthracnose caused by Colletotrichum capsici. Curcuma extracts and their essential oils were known as antifungal, but nonessential compounds have not been widely tested. This study aimed to assay the effectiveness of nonessential compounds of Curcuma longa, C. zedoaria, and C. aeruginosa to C. annuum. This study was conducted in November 2014 until Mei 2015 at Brawijaya University. The nonessential compound was obtained by soaking rhizome of C. longa, C. zedoaria, and C. aeruginosa in methanol, then distilled byusing rotary vacuum evaporator. Nonessential chemical compunds were identified by using HPLC. Effectiveness evaluation of nonessential compounds from three species of Curcuma was done by in vitro and in vivo test. Tested treatments were three species of Curcuma spp and 6 concentration levels of nonessential compounds (0 ppm, 4 ppm, 6 ppm, 8 ppm, 10 ppm, and 12 ppm). The xperiment was performed in Factorial Complete Randomized Design, with 18 treatments combination, and replicated three times. Results of HPLC analysis showed the rhizomes of the three Curcuma species contained curcumin and desmethoxycurcumin in various concentrations. The highest level was found in the C. longa extract (13.792 ppm curcumin and 67.156 ppm desmethoxycurcumin). However, in vitro test results showed nonessential compound of C. zedoaria was most effective in inhibiting C. annuum growth. The 10 ppm concentration inhibited 81.53 % of fungal growth. Further, the in vivo test, also indicated the same, it’s most effective in hampering the growth of anthracnose symptoms. Therefore, curcumin and desmethoxycurcumin from three species of Curcuma have potential to be developed as botanical fungicide.

POTENCY OF BLACK TEA FILTRATE AS UV PROTECTANT TO S/NPV JTM 97 C IN CONTROLLING HELICOVERPA ARMIGERA ON SOYBEAN Anella Retna Kumala Sari; Tiara Eka Ariestantia; Fery Abdul Choliq
Jurnal Penelitian Tanaman Industri Vol 26, No 1 (2020): June, 2020
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v26n1.2020.40-48

Helicoverpa armigera is the important pest on soybean which usually controlled by chemical insecticide spraying, so it should be replaced by naturally control using biological agents such as Spodoptera litura Nuclear Polyhedrosis Virus (S/NPV) JTM 97 C. It has some weakness such as during it’s applied in the field the effectiveness can become decreased after exposure the sunlight, especially ultraviolet (UV) so it needs the protective material, such as black tea filtrate which is known containing epigallocatechin gallate, caffeic acid, and apigenin which have role as UV protectors. This research aimed to investigate the potential of black tea filtrate to protect the S/NPV JTM 97 C from UV in controlling of H. armigera. The research was conducted in Pests and Diseases Laboratory on Indonesian Legumes and Tuber Crops Research Institute (ILETRI), Kendalpayak, Malang from January to June 2015. The research used Completely Randomized Design with seven treatments and four replications. They were S/NPV JTM 97 C without UV protectant as control (P1), S/NPV JTM 97 C + kaolin as comparison (P2), S/NPV JTM 97 C + black tea filtrate concentration 0.5 (P3), 1.0 (P4), 1.5 (P5), 2 (P6), and 2.5% (P7). Results showed that all of the protective materials of black tea filtrate were unable to provide mortality of H. armigera larvae better than kaolin. Kaolin can cause mortality of H. armigera larvae up to 82,50%. All of Black tea filtrate concentrations tested were not able to be used as protectant of S/NPV JTM 97 C due to it can only cause mortality of H. armigera up to 47,50% . Keywords: Entomopathogenic virus, LC50, protectant material, pest.

EFEKTIVITAS SENYAWA NONATSIRI DARI Curcuma spp. TERHADAP PENEKANAN PENYAKIT ANTRAKNOSA PADA BUAH CABAI Anella Retna Kumala Sari; Firdaus Auliya Rahmah; Syamsuddin Djauhari
Buletin Penelitian Tanaman Rempah dan Obat Vol 31, No 1 (2020): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v31n1.2020.21-30

One of the important diseases on chili is anthracnose caused by Colletotrichum capsici. Curcuma extracts and their essential oils were known as antifungal, but nonessential compounds have not been widely tested. This study aimed to assay the effectiveness of nonessential compounds of Curcuma longa, C. zedoaria, and C. aeruginosa to C. annuum. This study was conducted in November 2014 until Mei 2015 at Brawijaya University. The nonessential compound was obtained by soaking rhizome of C. longa, C. zedoaria, and C. aeruginosa in methanol, then distilled byusing rotary vacuum evaporator. Nonessential chemical compunds were identified by using HPLC. Effectiveness evaluation of nonessential compounds from three species of Curcuma was done by in vitro and in vivo test. Tested treatments were three species of Curcuma spp and 6 concentration levels of nonessential compounds (0 ppm, 4 ppm, 6 ppm, 8 ppm, 10 ppm, and 12 ppm). The xperiment was performed in Factorial Complete Randomized Design, with 18 treatments combination, and replicated three times. Results of HPLC analysis showed the rhizomes of the three Curcuma species contained curcumin and desmethoxycurcumin in various concentrations. The highest level was found in the C. longa extract (13.792 ppm curcumin and 67.156 ppm desmethoxycurcumin). However, in vitro test results showed nonessential compound of C. zedoaria was most effective in inhibiting C. annuum growth. The 10 ppm concentration inhibited 81.53 % of fungal growth. Further, the in vivo test, also indicated the same, it’s most effective in hampering the growth of anthracnose symptoms. Therefore, curcumin and desmethoxycurcumin from three species of Curcuma have potential to be developed as botanical fungicide.

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