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All Journal Agrikultura AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Nono Carsono
Universitas Padjadjaran
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Environmental Science

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Penggunaan Putresin Alami dan Sintetik untuk Induksi Kalus dan Regenerasi Kultur Anter Empat Genotipe Padi Nono Carsono; Desy Lidiasari; Santika Sari; Noladhi Wicaksana
Agrikultura Vol 32, No 3 (2021): Desember, 2021
Publisher : Fakultas Pertanian Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/agrikultura.v32i3.36784

Abstract

Teknik kultur antera sering digunakan untuk memperoleh galur-galur murni double haploid (DH) yang homozigot dalam waktu singkat, yang umumnya dicapai melalui proses induksi kalus. Putresin sebagai salah satu zat pengatur tumbuh dari poliamin diketahui sangat penting bagi perkembangan embriogenesis kalus. Tujuan dari penelitian ini adalah memperoleh plantlet dari penggunaan putresin sintetik dan alami pada media induksi dan regenerasi kalus kultur antera secara in vitro terhadap empat genotip-genotip F3 padi hasil persilangan (Oryza sativa L.). Eksplan yang digunakan adalah antera padi generasi F3 terseleksi hasil persilangan Caka, SP, Sair dan IRPW. Hasil penelitian menunjukan bahwa induksi kalus berhasil terbentuk hanya dari genotip Caka pada media N6 dengan penambahan putresin sintetik 10-3 M, persentase pembentukan kalus sebesar 16,1%. Kalus yang terbentuk dari genotip Caka memiliki struktur remah, kompak dan berwarna putih krem. Regenerasi menggunakan media dasar MS dengan penambahan putresin sintetik konsentrasi 10-3 M belum berhasil. Penambahan putresin sintetik lebih efektif dalam pembentukan kalus dibandingkan putresin ekstrak alami.
Karakterisasi Karakter Fisiologi Genotipe-Genotipe F2 Padi Ketan dengan Kemampuan Recovery Setelah Infeksi Tungro: Karakterisasi Karakter Fisiologi Genotipe-Genotipe F2 Padi Ketan dengan Kemampuan Recovery Setelah Infeksi Tungro Ema Komalasari; Fitri Widiantini; Santika Sari; Nono Carsono
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 3 No 2 (2019): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (499.2 KB) | DOI: 10.33019/agrosainstek.v3i2.67

Abstract

Tungro virus is one of the rice diseases which become one of limiting factors for rice production in Indonesia. The effective control can be done by using and rotation of resistant varieties. In order to develop tungro resistant varieties, hybridization has been conducted between susceptible (Ketonggo) and resistant variety (Utri Merah and ARC12596) i.e., Ketonggo x Utri Merah and Ketonggo x ARC12596. The main objective of the study was to characterize the physiological response of recovery genotypes group when compared to resistant genotypes. The genetic materials were F2 progenies of Ketonggo x Utri Merah and Ketonggo x ARC12596, each 230 genotypes. The experiment was conducted at BB Padi and Experimental Station of Faculty of Agriculture, Universitas Padjadjaran. Virus transmission was done using forced-tube inoculation method and symptoms scoring based on a standard evaluation system for rice. Traits observed were chlorophyll content, number of stomatal conductance, and quantum photosynthetic efficiency. The comparison of those traits between genotypes with recovery ability with those of resistant genotypes, susceptible genotypes, resistant variety check, and susceptible variety check was evaluated. It is found that recovered genotypes from both crossings did not show significant differences with those of resistant genotypes or resistant check variety on the above traits observed. Genotypes group with recovery ability can be used to suppress the spread of tungro disease.
Uji Daya Hasil dan Seleksi Famili Padi Generasi F4 dan F6 Hasil Persilangan Sintanur x PTB 33 dan Pandanwangi x PTB 33 di Jatinangor: Uji Daya Hasil dan Seleksi Famili Padi Generasi F4 dan F6 Hasil Persilangan Sintanur x PTB 33 dan Pandanwangi x PTB 33 di Jatinangor Zulfa Afifah; Nono Carsono; Santika Sari; Anas Anas
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 1 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (693.337 KB) | DOI: 10.33019/agrosainstek.v4i1.96

Abstract

High yielding is one of main objectives on rice breeding. A preliminary yield trial is one of steps in the selection process of developing new varieties. Selection for yield and its component traits is a complex process due to the nature of quantitative traits. The right selection approach may improve the effectiveness of the selection. It is therefore the objective of the current study was to select high yielding rice genotypes. A total of 10 promising rice families were evaluated and compared to four check varieties. Least Significant Increase (LSI), and selection index (Mulamba & Mock and Genotype Ideotype Distance index) analysis were performed. LSI test showed that PP48-5 and SP101-3-1-5 had the best performance in almost all traits compared to the lowest average of the check variety. The selection index found that SP101-3-1-5 had highest yield selection index and was recognized as superior family by selection index. SP101-3-1-5 is selected as potential family to be developed as a new high yielding rice varieties
Gene Pyramiding for Brown Planthopper Resistance-related Traits, Early Maturity and Aroma of Rice Assisted by Molecular and Phenotypic Markers Nono Carsono; Riski Gusri Utami; Santika Sari; Noladhi Wicaksana
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 4 No 2 (2020): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.33019/agrosainstek.v4i2.158

Abstract

The brown planthopper (BPH)-resistant rice, early maturity, and aromatic are expected by both farmers and consumers. The traits have been combined through gene pyramiding and some promising rice genotypes obtained. However the genetic control of BPH resistance and maturity is quantitatively inherited, it is therefore both molecular and phenotypic assessments would be tremendously helpful in selecting promising genotypes. The study aimed to obtain genotypes with such valuable traits. Rice lines were analyzed using molecular markers i.e., RM586, RM589, RM8213 (BPH resistant gene markers); RM7610 and RM19414 (early maturity markers), and IFAP (Internal Fragrant Antisense Primer) for detecting aromatic, and INSP (Internal Non-fragrant Sense Primer) for non-aromatic rice. Phenotypic assessment was performed for brown planthopper resistant-related traits, such as chlorophyll content, stomatal conductance, and trichome density. Other evaluations were heading date and aroma (using 1.7% KOH solution). Results showed that molecular markers for evaluating BPH resistance genes (Bph3, Bph4, Qbph4, and Bph17), aroma (fgr gene), and heading date (Hd2 and Hd3 genes) could differentiate genotypes, and they serve as perfect markers, except for heading date markers. Seven genotypes i.e., #2, #3, #4, #5, #6, #10, and #11 were related to all traits expected based on molecular marker analysis. Meanwhile, genotypes #1, #2, #4, #6, and #11 were similar to their parents based on phenotypic analysis. Pyramiding program based on molecular and phenotypic markers enables us to combine three valuable traits into one rice genotype as presented in this study
Co-Authors Anas Anas Desy Lidiasari Ema Komalasari Fitri Widiantini Noladhi Wicaksana Riski Gusri Utami Santika Sari Zulfa Afifah