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All Journal HAYATI Journal of Biosciences Jurnal Fitopatologi Indonesia Jurnal Penelitian Saintek Agrikultura Bionatura Cropsaver : Journal of Plant Protection Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Jurnal Agro Jurnal Perlindungan Tanaman Indonesia Kultivasi AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Soil REns Kumawula: Jurnal Pengabdian Kepada Masyarakat International Journal of Environmental, Sustainability, and Social Science Agrikultura Masyarakat Tani: Jurnal Pengabdian kepada masyarakat

Fitri Widiantini

Departemen Hama Dan Penyakit Tumbuhan, Fakultas Pertanian Universitas Padjadjaran

Author-ID : 415438

Agriculture, Biological Sciences & Forestry Arts Humanities Biochemistry, Genetics & Molecular Biology Decision Sciences, Operations Research & Management Earth & Planetary Sciences Economics, Econometrics & Finance Engineering Environmental Science Immunology & microbiology Languange, Linguistic, Communication & Media Medicine & Pharmacology Public Health Social Sciences

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Karakterisasi Karakter Fisiologi Genotipe-Genotipe F2 Padi Ketan dengan Kemampuan Recovery Setelah Infeksi Tungro Komalasari, Ema; Widiantini, Fitri; Sari, Santika; Carsono, Nono
AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian Vol 3 No 2 (2019): AGROSAINSTEK: Jurnal Ilmu dan Teknologi Pertanian
Publisher : Universitas Bangka Belitung

Penyakit pada padi, salah satunya tungro masih menjadi pembatas utama produksi padi di Indonesia. Pengendalian efektif dapat dilakukan melalui penggunaan dan pergiliran varietas tahan. Tujuan penelitian ini untuk mengetahui fisiologi genotipe-genotipe yang mengalami recovery setelah serangan tungro dibandingkan dengan genotipe-genotipe tahan. Penelitian dilakukan pada dua populasi generasi kedua persilangan Ketonggo x Utri Merah dan Ketonggo x ARC12596 masing-masing 230 genotipe di rumah kaca BB Padi dan Kebun percobaan Universitas Padjadjaran. Inokulasi virus pada tanaman dilakukan dengan menggunakan forced-tube inoculation dan skoring gejala berdasarkan sistem evaluasi standar untuk padi. Pengamatan kandungan klorofil, jumlah konduktansi stomata, dan kuantum efisiensi fotosintesis dilakukan dengan membandingkan antara grup genotipe recovery dengan grup genotipe tahan dan grup genotipe rentan, serta varietas cek tahan, dan varietas cek rentan. Hasil penelitian menunjukkan bahwa secara umum genotipe-genotipe recovery dari persilangan Ketonggo x Utri Merah tidak memperlihatkan perbedaan yang signifikan dengan genotipe tahan maupun cek tahan pada pengamatan kandungan klorofil, konduktansi stomata, dan kuantum efisiensi fotosintesis. Hasil yang sama juga diperoleh untuk persilangan Ketonggo x ARC12596. Genotipe-genotipe yang mengalami recovery dari kedua persilangan memiliki morfologi dan fisiologi yang sama baiknya dengan genotipe tahan dan cek tahan sehingga dapat digunakan untuk menekan penyebaran penyakit tungro.

Resistance of Nilaparvata lugens to Fenobucarb and Imidacloprid and Susceptibility to Neem Oil Insecticides Danar Dono; Neneng S Widayani; Safri Ishmayana; Yusup Hidayat; Fitri Widiantini; Ceppy Nasahi
HAYATI Journal of Biosciences Vol. 29 No. 2 (2022): March 2022
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.29.2.234-244

This study aims to monitor and determine the level of resistance of N. lugens to fenobucarb and imidacloprid, to analyse of the specific activities of asetylcholinesterase, Glutation S-Transferase, esterase, and to determine sensitivity of resistant N. lugens to the neem oil. Resistance tests were conducted on a field population from Cipunagara, Subang, West Java, Indonesia, and a standard population originating from Banyuwangi, East Java, that was obtained from the Indonesian Center for Rice Research. Synthetic and neem Insecticides were applied using the leaf-stem dipping method. The resistance ratio obtained by comparing the LC50 value of the field population with the standard. The test insects were considered to be resistant to synthetic insecticide if they had a resistance ratio (RR) of more than 4, indicated resistance if RR ≥1, and susceptible to neem if has RR <1. The results of the research showed that the N. lugens population of Cipunagara indicated resistance to fenobucarb (RR = 2.43), while it was clearly resistance to imidacloprid (RR = 13.95). Specific activities of the acetylcholinesterase and Glutathione S-Transferase of the N. lugens Cipunagara populations were higher than the standard, while the esterase specific activity was lower. These two enzymes are responsible for the resistance of N. lugens to fenobucarb and imidacloprid. Nevertheless, the N. lugens population of Cipunagara was susceptible to neem (RR <1) that never been reported before. Therefore, it is important to know the change in the sensitivity of the target and the metabolic pathway of the insecticide used to manage insect resistance.

Potensi Metabolit Sekunder Asal Bakteri Endofit dalam Menekan Pertumbuhan Miselium Ganoderma boninense Fitri Widiantini; Ceppy Nasahi; Endah Yulia; Serly Noviyawati
Jurnal Fitopatologi Indonesia Vol. 14 No. 3 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Potency of Endophytic Bacterial Secondary Metabolite to Inhibit Mycelium Growth of Ganoderma boninense Endophytic bacteria have a close relationship with their host plants. The bacteria are living inside their host without causing any symptom. Endophytic bacteria are well known for their ability to produce compounds that can be used to protect plants from deleterious microorganisms. The research was conducted to determine the ability of endophytic bacteria in producing antifungal compounds that are able to suppress the growth of Ganoderma boninense, a causal agent of basal stem rot disease of oil palm. Previously isolated endophytic bacteria were grown on international streptomyces project 2 (ISP2) liquid medium and incubated at rotary evaporator at 120 rpm for 10 days. The supernatant was separated from bacterial cells and tested against G. boninense using well diffusion agar method. The results showed that endophytic bacteria isolate of BEK6, BEK7, BEK9 and BEK10 were able to produce antifungal compound against G. boninense. Microscopic observation demonstrated G. boninense mycelia alteration following secondary metabolite application.

Ekstrak Tanaman Binahong sebagai Pengendali Penyakit Hawar Pelepah Daun Padi Endah Yulia; Fitri Widiantini
Jurnal Fitopatologi Indonesia Vol 14 No 4 (2018)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Binahong Extract for Controlling Sheath Blight Disease of Rice PaddySheath blight disease, caused by Rhizoctonia solani, is an important disease that can affect rice production. The disease is commonly controlled using synthetic fungicides. This study was aimed to test the potential of binahong plant (Anredera cordifolia) as botanical fungicide to inhibit the growth of R. solani and the development of blight disease on rice seedlings. The research was conducted in 2 stages, i.e. in vitro and rice seedling testing. In vitro test was carried out on PDA medium containing different concentrations of binahong leaf extract (0.125%, 0.25%, 0.5%, 1%, and 2%). Rice seedling testing was completed by soaking rice seeds in five concentrations of binahong leaf extract followed by inoculation of R. solani. The experiments were arranged in a completely randomized design. The variables observed consist of inhibitory ability of binahong leaf extract, colony morphology of R. solani, and disease incidence. Binahong leaf extract inhibited 35.2% growth of R. solani colonies. Treatment of binahong leaf extract had some effects on mycelium of R. solani; macroscopically the mycelium was shorter, thinner and congregated, whereas microscopically it was smaller, elongated and tended to be colourless compared to control. The treatment of 2% binahong leaf extract on rice seeds decreased the incidence of seedling blight disease with the effectiveness reached 100%. Thus, binahong leaf extract has the potential to suppress sheath blight disease caused by R. solani.

Variasi Morfometri dan Patogenisitas Peronosclerospora spp. Penyebab Penyakit Bulai Jagung di Pulau Jawa, Indonesia Satriyo Restu Adhi; Fitri Widiantini; Endah Yulia
Jurnal Fitopatologi Indonesia Vol 17 No 5 (2021)
Publisher : The Indonesian Phytopathological Society (Perhimpunan Fitopatologi Indonesia)

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14692/jfi.17.5.173-182

Morphometric and Pathogenecity Variation of Peronosclerospora spp. The Causal Agent of Maize Downy Mildew in Java Island, Indonesia Downy mildew disease in maize caused by Peronosclerospora spp. has been reported to cause yield loss in several production centers in Java. This study aimed to determine the morphometric characteristics and pathogenicity of Peronosclerospora spp.. Ten strains of Peronosclerospora were collected from maize production center in Blitar (BLT), Kediri (KDR), Kediri 2 (KDR2), Klaten (KLT), Cianjur (CJR), Garut (GRT), Jatinangor Sumedang (JTN), Rancakalong Sumedang (RCG), Indramayu (IMY), and Sukabumi (SKB). Morphometric variation was determined using microscope by observing shape of conidium; measuring cell wall thickness, length of conidiophores, dimensions of conidia; and counting the number of branches. Differences in morphology and pathogenicity between strains was evidenced. Dendogram analysis based on morphometric characters differentiated strains of Peronosclerospora into 2 main clusters. One strain, KDR2 is in the same group and identical with reference strain P. philippinensis; while the others are in the same group and identical with reference strain P. maydis. Pathogenicity test showed that IMY strain caused the lowest disease incidence (8.33%) and KLT strain caused the highest disease incidence (47.92%).

Assessment of Sarocladium oryzae Growth Inhibition, the Causal Agent of Rice Sheath Rot Disease, Using Methanol Extract of Binahong Leaves Endah Yulia; Silviya Rahma Dhiya Syafira; Fitri Widiantini; Wawan Kurniawan
CROPSAVER Vol 2, No 1 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Sheath rot disease caused by Sarocladium oryzae is an important disease on rice. The pathogen that infects uppermost leaf sheath enclosing to the young panicles can cause yield losses up to 85%. Though the disease is commonly controlled using synthetic fungicides, the use of natural products that safer to the environment have been enormously explored. The research aimed to test growth inhibition of S. oryzae colony using methanol leaf extract of binahong (Anredera cordifolia). The research was carried out from June until December 2017 at the Laboratory of Biotechnology Plant Protection, Department of Plant Pest and Diseases, Faculty of Agriculture, Universitas Padjadjaran. The method used was Randomized Complete Design consisted of six treatments and four replications. The treatments were five concentrations of binahong leaf extract of 0.125%, 0.25%, 0.5%, 1%, 2% and one control treatment. The result showed that binahong leaf extract did not inhibit the growth of S. oryzae. The highest inhibition percentage of 4.55% at 0.25% concentration was considered to be a low inhibition category. Although the colony growth of S. oryzae was not significantly inhibited, the extract caused hyphal abnormalities. This actually shows the antifungal potency of the binahong leaf extract that needed further examination with more adequate methods.Keywords : Anredera cordifolia, botanical pesticide, rice sheath rot

Potency of Bacillus subtilis and Lysinibacillus Mixed With Silica Nano Particles and Carbon Fiber in Controlling Potato Leaf Blight Disease [Phytophthora infestans (Mont.) de Bary] Hersanti Wartono; Fitri Widiantini; Kirana Sonya Harviana
CROPSAVER Vol 2, No 2 (2019)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Leaf blight disease caused by Phytohthora infestans is one of the major diseases on potato plant. To control the disease farmers usually use synthetic fungicides. However, the excessive use of synthetic fungicides may cause negative impacts on the environment. One of the alternative control methods which is more environtmentally friendly is the use of antagonistic microbes. In this experiment, Bacillus subtilis and Lysinibacillus sp. were formulated with carbon fiber as a carrier and enriched with nano silica as a micro nutrient, to find out the ability of the bacteria in suppressing leaf blight disease on potato plant. The experiment was carried out in the experimental field in Lembang, Bandung Barat, Jawa Barat. The experiment was arranged in the randomized block design consisted of nine treatments and four replications. Density of B.subtilis and Lysinibacillus sp. were 1011 cfu/ml. The formulation was applied 3 times, on planting, 2 weeks and 4 weeks after planting. The results showed that the application of B. subtillis and Lysinibacillus sp. were able to suppress leaf blight disease, with the disease inhibition 36,6% and 32,7% respectively. The combination treatment of B. subtilis in silica nano and carbon fiber was able to suppress leaf blight disease on potato plant higher compared to the application of bacteria without nano silica and carbon fiber, with the disease inhibition up to 51,2%.

EndophyticBbacteria origin of healthy rice plants produce antifungal volatile compound inhibited the growth of Pyricularia oryzae Cav., the causal agent of rice blast disease Fitri Widiantini; Fuji Hartati
CROPSAVER Vol 3, No 1 (2020)
Publisher : Departemen Hama dan Penyakit Tumbuhan Fakultas Pertanian Universitas Padjadjaran

Endophytic bacteria have been known as producers for bioactive compound in which widely used in the field of crop protection. Several isolates of endophytic bacteria have demonstrated their potential ability as biocontrol agents by inhibiting the development of major rice pathogens. However, their ability to produce bioactive compounds has not been explored. The study reported the ability of the endophytic bacteria isolates in producing antifungal volatile compounds to inhibit the growth of Pyricularia oryzae Cav., the causal agent of rice blast disease. The experiment was conducted by dual culture method using bipartite compartments in which the bacterial isolates and P. oryzae were grown separately and placed by facing to each other. The bacterial isolates were grown on ISP2 agar media, whereas P. oryzae was grown on PDA media. Nine isolates were tested for their ability in producing antifungal volatile compound. The potential of antifungal volatile compound was observed by the P. oryzae growth compared to control. Furthermore, its effect on the P. oryzae mycelia was observed under microscope. The result demonstrated that all of the tested isolates were able to produce volatile compound which were able to inhibit the growth of P. oryzae. In addition, the compounds were also causing mycelial alteration.

Pengujian Filter Fisik (Slow Sand Filter) Untuk Menurunkan Kadar Pestisida Golongan Organoklorin Siska Rasiska; Aditya Bintan Pratama; Fitri Widiantini
Soilrens Vol 15, No 1 (2017)
Publisher : Fakultas Pertanian Universitas Padjadjaran

Organochlorine pesticide is one of the pesticide that has high persistency and toxic. One of the attempt to degrade organochlorine pesticide using cheaper and easier way is using the slow sand filter technology. The research aimed to test the slow sand filter and to obtain the best filter media combination for degrading organochlorine pesticide. The experiment was conducted at the Laboratory of Pesticide and Toxicology, Laboratory of Plant Protection Biotechnology and Laboratory of Chemical and Soil Fertility, Faculty of Agriculutre, Padjadjaran Univeristy from December 2016 – May 2017. The experimental design used the observative and descriptive design with 8 treatments and repeated twice. P1 (activated carbon and gravel), P2 (sand and gravel), P3 (silica sand and gravel), P4 (activated carbon and zeolite), P5 (sand and zeolite), P6 (silica sand and zeolite), P7 (activated carbon, silica sand and zeolite), P8 (activated carbon, sand and gravel). The result showed that all treatments of slow sand filter were able to degrade organochlorine pesticide and the best filter media combination was from P7 with ability to degrade the pesticide level by 82,86%.Key words: slow sand filter, activated carbon, silica sand, sand, gravel, zeolite

MANAJEMEN APLIKASI PESTISIDA TEPAT DAN BIJAKSANA PADA KELOMPOK TANI PADI DAN SAYURAN DI SPLPP ARJASARI Endah Yulia; Fitri Widiantini; Agus Susanto
Kumawula: Jurnal Pengabdian Kepada Masyarakat Vol 3, No 2 (2020): Kumawula: Jurnal Pengabdian Kepada Masyarakat
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/kumawula.v3i2.27459

SPLPP Arjasari di Desa Arjasari, Kabupaten Bandung merupakan tempat pelatihan dan pengembangan pertanian yang dimiliki Universitas Padjadjaran. Kelompok tani di bawah arahan SPLPP Arjasari banyak menanam komoditas tanaman pangan dan sayuran seperti padi, jagung, kedelai, cabai, tomat, dan buncis. Namun demikian, keberadaan organisme pengganggu tanaman (OPT) menjadi salah satu masalah utama bagi petani dimana pengendaliannya masih bergantung pada penggunaan pestisida kimia. Permasalahannya adalah sebagian besar petani masih melakukan aplikasi pestisida sintetik secara rutin serta sering menggunakan dosis yang tidak sesuai anjuran. Hal tersebut telah menimbulkan beberapa kerugian seperti mahalnya biaya pengendalian OPT, tidak efektifnya pestisida yang digunakan dan kemungkinan timbulnya efek lain seperti resistensi, residu, serta pencemaran lingkungan. Tujuan dari kegiatan ini adalah untuk meningkatkan pemahaman, keahlian dan keamanan petani dalam pengaplikasian pestisida di Kelompok Tani Arjasari. Metode yang digunakan dalam kegiatan pengabdian ini adalah metode pendekatan kelompok melalui penyuluhan, pelatihan partisipatif, dan studi lapangan dengan mengintegrasikan antara teori dan praktek. Pelaksanaan dilakukan dalam bentuk ceramah, instruksional, diskusi, dan praktek lapang. Petani diajarkan teknik pengendalian OPT secara tepat dan bijaksana, ekologi dan biologi OPT, teknik aplikasi pestisida dan kalibrasi alat, bahaya pestisida baik bagi jasad non target, bagi dirinya dan orang lain, serta gejala akibat paparan pestisida dan cara pertolongan pertamanya. Melalui kegiatan ini petani dapat menambah pengetahuan dan pemahaman di dalam upaya peningkatan keberhasilan pengendalian OPT yang tepat dan bijaksana di SPLPP Arjasari, sehingga akan pula meningkatkan kualitas dan kuantitas produksi pertanian mereka.SPLPP Arjasari in Arjasari Village, Bandung Regency is a place for training and agricultural development owned by Universitas Padjadjaran. Farmer groups under the direction of SPLPP Arjasari are grown quite diverse food crops and vegetables such as rice, corn, soybeans, chillies, tomatoes, and beans. However, the presence of plant pests dan diseases becomes one of the main problems for farmers. The most common control method using by farmers is the use of chemical pesticides. The problem is that most farmers still routinely apply synthetic pesticides and often use doses that are not as recommended. This has caused several disadvantages such as the high cost of pest control, the ineffectiveness of pesticides used and the possibility of other effects such as resistance, residue, and environmental pollution. The purpose of this activity was to increase the awareness, expertise and safety of farmers in the application of pesticides in the Arjasari Farmers Group. The method used in this community service was the group approach method through counseling, participatory training, and field studies by integrating theory and practice. Activity implementation was carried out in the form of lectures, instructional, discussion, and field practice. Farmers were taught proper and wise pest control techniques, ecology and biology of pests and pathogens, pesticide application techniques and tool calibration, the dangers of pesticides both for non-target organisms, for themselves and others, as well as symptoms due to pesticide exposure and how to perform first aids. Through this activity, farmer knowledge and understanding in increasing the success of appropriate and wise pest control in the Arjasari SPLPP will be increased, so that it will also improve the quality and quantity of their agricultural production.

Co-Authors Adelia Septiandini Aditya Bintan Pratama Agung Karuniawan Agung Kurniawan Agus Susanto Agus Susanto Agus Susanto Aina Anna Roosda Andang Purnama Andang Purnama Andang Purnama Asri Peni Wulandari Avissa Ayuningdiyas Betty Natalie Fitriatin Ceppy Nasahi Citra Bakti, Citra Danar Dono Danar Dono Deni Ariyono Dewi, Vira Kusuma Dinda Sekar Ayu Fiko Dwindry Formanda Dwisari Januarily Pitaloka Elga Sari Ema Komalasari Ema Komalasari, Ema Emma Trinurani Sofyan Endah Yulia Endah Yulia Endah Yulia Endah Yulia Yulia Fajri Maulana Anugrah Fakhrurroja, Hanif Feliatra Fiko, Dinda Sekar Fitri, Elysa Fuji Hartati Fuji Hartati Ghifari, Raden Faqih Hilmiy Hadi Syahir Muhadam Hersanti - Hersanti HERSANTI Hilda Sandra Utami Ida Nurhelawati Ida Nurhelawati Ifa Dwi LUTHFIANA Kang, Seung Won Kartika Sari Kirana Sonya Harviana Krissanti, Petra Sulistya Dian Lindung Tri Puspasari Luciana Djaya, Luciana Margaretta Christita, Margaretta Mia Rahmah Qadryani Mitalo, Oscar W. Muhammad Maksum Nabilla, Anisa Neneng S Widayani Nia Rossiana Nono Carsono Nono Carsono Noor Istifadah Noor Istifadah Nugraha, Gema Takbir Parakkasi, Karmilla Prabowo, Yudhisa Hendri Putra, Fitriansyah Putu Ghita Novilaressa Ramdan Firmansah Rangga Irawan Prasetyo RIKA MELIANSYAH Riswandi, Hafiz Rizqullah, Ahmad Fauzan Safri Ishmayana Santa Monica Santika Sari Satriyo Restu Adhi Serly Noviyawati Silviya Rahma Dhiya Syafira Siska Rasiska SIska Rasiska, SIska Sri Hartati Sri Hartati Sudarjat Sudarjat Sukmono Suwignyo Susanti, Rista Syahnur, Fitika Syarif Hidayat Tarkus Suganda Tati Herlina Tiara Febricyanti Putri Senjaya Tualar Simarmata Vira Kusuma Dewi Vira Kusuma Dewi Wahyu Daradjat Natawigena, Wahyu Daradjat Wawan Kurniawan Yulia, Endah Yulia Yunira, Alma Yusup Hidayat Yusup Hidayat

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