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All Journal Jurnal AgroBiogen
Sustiprijatno Sustiprijatno
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
Agriculture, Biological Sciences & Forestry

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Transformasi Genetik Tembakau dengan Gen Cold Shock Protein melalui Perantara Agrobacterium tumefaciens Seagames Waluyo; Sustiprijatno Sustiprijatno; Suharsono Suharsono
Jurnal AgroBiogen Vol 9, No 2 (2013): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n2.2013.p58-65

Abstract

Coldshock protein (Csp) essential for organisms to survive inabiotic stress condition. CspB gene has been fused toubiquitin promoter in the T-DNA region of pCambia 1300int,and introduced into Agrobacterium tumefaciens LBA4404.This research had an objective to transform geneticallyNicotiana tabacum cv. Samsun by CspB gene under thecontrol of Ubiquitin promoter and NOS terminator mediatedby A. tumefaciens. Leaf discs were co-cultivated with A.tumefaciens LBA 4404. Based on the number of hygromycinresistantcalli, the efficiency of transformation was 57.5%. Inthe selective medium containing 50 μg/l hygromycin, theefficiency of regeneration of transgenic shoots was 82.6%.Based on PCR analysis using primers corresponding toubiquitin promoter and CspB gene, 18 putative tobaccotransgenic containing CspB gene under the control ofubiquitin promoter.
Co-Authors . SUHARSONO Seagames Waluyo