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All Journal HAYATI Journal of Biosciences Jurnal Ilmu Dasar Indonesian Journal of Forestry Research Jurnal AgroBiogen Indonesian Journal of Biotechnology BERITA BIOLOGI Jurnal Penelitian Karet Jurnal Riset Akuakultur Berkala Penelitian Hayati
. SUHARSONO
Departemen Biologi Fakultas MIPA Institut Pertanian Bogor Kampus IPB Darmaga Bogor Jawa Barat
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Control & Systems Engineering Earth & Planetary Sciences Education Environmental Science Immunology & microbiology Materials Science & Nanotechnology Mathematics Medicine & Pharmacology

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Physiological and Biochemical Responses to Aluminum Stress in the Root of a Biodiesel Plant Jatropha curcas L. RADITE TISTAMA; UTUT WIDYASTUTI; DIDY SOPANDIE; AKIHO YOKOTA; KINYA AKASHI; . SUHARSONO
HAYATI Journal of Biosciences Vol. 19 No. 1 (2012): March 2012
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (175.274 KB) | DOI: 10.4308/hjb.19.1.37

Abstract

We investigated J. curcas responses to aluminum stress, histochemically and biochemically. Histochemical stainings were observed to analysis aluminum accumulation, lipid peroxidation and the loss of plasma membrane integrity on the surface and tissue of the root apex. Enzymatic analysis was conducted to measure malate content in leaf, root and malate efflux in the medium. We used M. malabathricum as a comparison for Al-tolerance plant. J. curcas root elongation was inhibited by 0.4 mM AlCl3, while M. malabathricum root elongation was inhibited by 0.8 mM AlCl3 treatment. Inhibition of root elongation has high correlation with Al accumulation in the root apex, which caused lipid degradation and cell death. Generally, malate content in J. curcas leaf and root was higher than that in M. malabathricum. In the contrary malate efflux from the root into the medium was lower. J. curcas root has a different pattern compared to M. malabathricum in malate synthesis and malate secretion when treated with a different Al concentration. We categorized J. curcas acc IP3 as more sensitive to aluminum than M. malabathricum.
DISTRIBUTION OF Hoya multiflora Blume AT GUNUNG GEDE PANGRANGO NATIONAL PARK, INDONESIA Sri Rahayu; Cecep Kusmana; Rochadi Abdulhadi; Muhammad Jusuf; Suharsono Suharsono
Indonesian Journal of Forestry Research Vol 7, No 1 (2010): Journal of Forestry Research
Publisher : Secretariat of Agency for Standardization of Environment and Forestry Instruments

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20886/ijfr.2010.7.1.42-52

Abstract

Hoya multiflora is one of the valuable germplasm in Indonesia that has been utilized as ornamental and medicinal plant. This epiphytic plant faces problems in decreasing habitat. As a means for developing a habitat framework for describing the distributions and ecological relations of H.multiflora at Gunung Gede Pangrango National Park, Bogor, Indonesia, ecological study of this species was established over the ranges of altitudes and characteristic vegetation structural types (primary and secondary forest, and plantation) present in the Park. Recognizing the fact that such study requires multidisciplinar y data, this paper explores the evidences from both herbarium sheets and field observations. The result of the study showed that the population of this species was only found at the Bodogol Research Station at elevation of 700 - 900 m above sea level (a.s.l.). Thus, the facts contradict with the evidence from the herbarium sheets of the Herbarium Bogoriense which have presumed that this species has a wide variation of altitudinal range from 20 to 1500 m a.s.l. (Indonesia) or 200 - 1400 m a.s.l. ( Java). The Bodogol’s population showed the clumped type of dispersion (Morisita’s Index = 1.35), which indicated such environment that was characterized by patchy resources. Direction and speed of wind coupled with the topography are ecological factors that affect to the distribution of this parachute typed seeds of the H.multiflora.
Transformasi Genetik Tembakau dengan Gen Cold Shock Protein melalui Perantara Agrobacterium tumefaciens Seagames Waluyo; Sustiprijatno Sustiprijatno; Suharsono Suharsono
Jurnal AgroBiogen Vol 9, No 2 (2013): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n2.2013.p58-65

Abstract

Coldshock protein (Csp) essential for organisms to survive inabiotic stress condition. CspB gene has been fused toubiquitin promoter in the T-DNA region of pCambia 1300int,and introduced into Agrobacterium tumefaciens LBA4404.This research had an objective to transform geneticallyNicotiana tabacum cv. Samsun by CspB gene under thecontrol of Ubiquitin promoter and NOS terminator mediatedby A. tumefaciens. Leaf discs were co-cultivated with A.tumefaciens LBA 4404. Based on the number of hygromycinresistantcalli, the efficiency of transformation was 57.5%. Inthe selective medium containing 50 μg/l hygromycin, theefficiency of regeneration of transgenic shoots was 82.6%.Based on PCR analysis using primers corresponding toubiquitin promoter and CspB gene, 18 putative tobaccotransgenic containing CspB gene under the control ofubiquitin promoter.
Method development for detection of E545A mutation PIK3CA gene in breast cancer patients using Tm Shift SYBR Green I qPCR Fuad Al Ahwani; Desriani Desriani; Utut Widyastuti; Suharsono Suharsono
Indonesian Journal of Biotechnology Vol 21, No 1 (2016)
Publisher : Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (987.933 KB) | DOI: 10.22146/ijbiotech.26815

Abstract

E545A is one of the point mutations, and its frequency is high in PIK3CA gene (3.8%), particularly breast cancer patients in Singapore (13.8%) and Mexico (11.5%). In addition to induce breast cancer, the mutation also caused resistance of anti-HER2 in HER2 cancer subtype. The tremendous effect of this mutation was not supported by affordable detection method. This study aimed to develop a feasible and sensitive method of E545A detection. The developing method used Tm and Ct to identify samples. Based on optimization, the best condition was obtained at optimization 2 at annealing temperature of 65°C. At this condition, Tm and Ct of each sample were (a) exon 9 (78.4°C and 13.005±0.007) and (b) E5454A (80.4°C and 10.07±0.1). This method also demonstrated good precision as observed in variance coefficient of intra and inter assay (0). Thus, method for E5454A detection mutation was successfully developed.
REKAYASA EKSPRESI GEN PEMBUNGAAN Hd3a DIBAWAH KENDALI PROMOTER ROL C PADA JARAK PAGAR (Jatropha curcas L.) Yohana C Sulistyaningsih; Alex Hartana; Utut Widyastuti; Hamim Hamim; Suharsono Suharsono
BERITA BIOLOGI Vol 10, No 6 (2011)
Publisher : Research Center for Biology-Indonesian Institute of Sciences

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14203/beritabiologi.v10i6.1940

Abstract

Flowering in jatropha (Jatropha curcas L.) was considered as one of major factors that contribute to its productivity. Small number of female flowers produced in each inflorescence was believed as the main cause of low seed production.Introduction of Hd3a flowering gene driven by rol C promoter was supposed to improve total number of flowers including female flower.The objective of this research was to optimize cell proliferation and regeneration medium in Jatropha transformation method mediated by Agrobacterium, to obtain transgenic Jatropha containing Hd3a flowering gene as well as to understand the effect of this transgene on jatropha flowering character.Callus induction medium containing 0.5 mg/1 IBA added with 3 g/1 PVP produced the highest frequency of shoot formation.We obtained 26.67% to 33.33% putative transgenic plantlets that were able to grow in 40mg/l hygromycin selection medium. PCR analysis revealed that seven out of 10 putative transgenic plantlets were positively transgenic.Extremely early flowering character that was confirmed by histological analysis was also shown by some transgenic plantlets.
ISOLASI DAN KARAKTERISASI GEN SITRAT SINTASE BAKTERI Pseudomonas aeruginosa DARI FILOSFER Hevea brasiliensis Muell. Arg. Radite Tistama; Utut Widyastuti; Suharsono Suharsono
Jurnal Penelitian Karet JPK : Volume 31, Nomor 2, Tahun 2013
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v31i2.140

Abstract

Pseudomonas aeruginosa merupakan bakteri utama di dalam rizosfer yang mempunyai sifat-sifat yang dapat dimanfaatkan di dalam pertanian dan lingkungan. Bakteri tersebut mensekresikan asam organik yang dapat melepaskan fosfor dan melindungi akar dari keracunan aluminium. Sitrat merupakan asam organik yang dominan disekresikan oleh Pseudomonas di dalam tanah. Sitrat menujukkan afinitas terhadap aluminium dan menyediakan fosfor yang lebih tinggi dibandingkan asam organik lainnya. Asam organik ini disintesis dai sebuah reaksi antara aksaloasetat dan asetil KoA, dikatalisis oleh sitrat sintase (CS) di dalam siklus Kreb. Penelitian ini dilakukan untuk mengisolasi dan mengkarakterisasi sitrat sintase dari Pseudomonas aeruginosa yang telah diisolasi dari permukaan daun tanaman karet. Primer spesifik untuk gen CCS didesain berdasarkan sekuen gen sitrat sintase beberapa bakteri yang disimpan di Genbank. Primer tersebut digunakan untuk mengamplifikasi gen CS dengan menggunakan mesin PCR. Gen CS telah berhasil diisolasi dari bakteri filosfere Pseudomonas aerugunosa. Gen CS Pseudomonas aeruginosa (PaCS) tersebut terdiri dari 1287 pb dan menyandikan 428 asam amino.  PaCS mempunyai kesamaan asam amino yang tinggi dan hidrofobisitas dengan CS bakteri lainnya dan diduga mempunyai persamaan aktivitas enzim. Diterima : 11 April 2013; Disetujui : 17 September 2013  How to Cite : Tistama, R., Widyastuti, U., & Suharsono. (2013). Isolasi dan karakterisasi gen sitrat sintase bakteri Pseudomonas aeruginosa dari filosfer Hevea brasiliensis Muell. Arg.. Jurnal Penelitian Karet, 31(2), 127-138. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/140  
TUMPANGSARI SORGUM DAN KEDELAI UNTUK MENDUKUNG PRODUKTIVITAS LAHAN TBM KARET (HEVEA BRASILIENSIS MUELL ARG) Radite Tistama; Cici Indriani Dalimunthe; YanRiska Venata Sembiring; Iif Rahmat Fauzi; Ratih Dewi Hastuti; Suharsono Suharsono
Jurnal Penelitian Karet JPK : Volume 34, Nomor 1, Tahun 2016
Publisher : Pusat Penelitian Karet - PT. Riset Perkebunan Nusantara

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.22302/ppk.jpk.v34i1.222

Abstract

Penanaman sorgum (Sorghum bicolor) dan kedelai (Glycine max) sebagai tanaman tumpangsari merupakan pilihan yang tepat untuk mendukung upaya pengembangan pertanian berkelanjutan dan peningkatan produksi pangan Indonesia.  Lahan karet belum menghasilkan cukup luas untuk dimanfaatkan untuk upaya tersebut. Penelitian ini bertujuan untuk mendapatkan pola tanam tumpangsari yang tepat dan melihat interaksinya terhadap tanaman karet baik dalam hal penyebaran penyakit jamur akar putih dan kesuburan tanah. Penelitian ini menggunakan Rancangan Acak Kelompok (RAK) dengan dua faktor perlakuan dengan tiga ulangan. Faktor perlakuan yang digunakan yaitu jarak tanaman tumpangsari 0,5 m, 1 m dan 1,5 m terhadap tanaman karet, dan jenis tanaman tumpangsari yaitu sorgum dan kedelai. Penelitian dilakukan di gawangan tanaman karet umur 1 tahun (TBM 1) dan umur 3 tahun (TBM 3). Hasil penelitian menunjukkan bahwa pengaruh jarak tanaman tumpangsari pada setiap perlakuan tidak berbeda nyata terhadap pertumbuhan tanaman karet. Tanaman tumpangsari sorgum dan kedelai meningkatkan pH, fosfor, nitrogen, dan kapasitas tukar kation (KTK) di dalam tanah, serta dapat menekan penyebaran penyakit Jamur Akar Putih (JAP). Produksi tumpangsari menunjukkan pola tanam kedelai dan sorgum terbaik pada jarak tanam 0,5 m dari tanaman karet dan tumpangsari sorgum dan kedelai (tunggal) pada TBM 1 dapat memberikan keuntungan serta nilai tambah bagi usahatani karet. Diterima : 6 Januari 2016 / Direvisi : 20 Juli 2016 / Disetujui : 30 Juli 2016 How to Cite : Tistama, R., Dalimunthe, C., Sembiring, Y., Fauzi, I., Hastuti, R., & Suharsono, S. (2016). Tumpangsari sorgum dan kedelai untuk mendukung produktivitas lahan TBM Karet (Hevea brasiliensis Muell Arg). Jurnal Penelitian Karet, 0, 61-76. Retrieved from http://ejournal.puslitkaret.co.id/index.php/jpk/article/view/222
Screening of Genomic Library of Soybean Cultivar Lumut by Using Peroxidase Gene from Arabidopsis thaliana as Probe Suharsono Suharsono; Teguh Juliyanto; Muhammad Jusuf
Jurnal ILMU DASAR Vol 11 No 1 (2010)
Publisher : Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Jember

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (310.269 KB)

Abstract

Screening to genomic library of soybean cv. Lumut by using gene encoding for peroxidase (per) from A. thaliana as a probe has an objective isolate the whole gene of per from soybean. The probe was labeled by non-radioisotope alkalin phosphatase. Screening was done by two steps. The first, screening was done to 105 recombinant lambda phages containing genome of soybean cv. Lumut. After southern hybridization, positive signal of plaques were isolated and screened for the second time. After second screening, some recombinant lambda phages containing putatively per genes were isolated. Excision from recombinant lambda phages into recombinant plasmid was successfully done in Escherichia coli strain BM25.8. The plasmid DNAs were isolated from E. coli strain BM25.8 and introduced into E. coli strain DH5α for multiplication. Plasmid DNAs were digested by EcoRI and transferred onto nylon membrane hybond N+. Southern hybridization analysis showed that one clone, L10/R/3/4, contain per gene in the 7.7 kb EcoRI fragment. This fragment is inserted into pSportI. 
Regeneration and histological study of somatic embryogenesis of sugarcane (Saccharum officinarum L.) cultivar PS 864 Fitri Damayanti; Suharsono Suharsono; Aris Tjahjoleksono; Ika Mariska
JURNAL PENELITIAN BIOLOGI BERKALA PENELITIAN HAYATI Vol 24 No 1 (2018): December 2018
Publisher : The East Java Biological Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (330.917 KB) | DOI: 10.23869/36

Abstract

The process affecting somatic embryo formation in sugarcane is very specific for each genotype, so the determination of the best somatic embryo regeneration medium in sugarcane clones is necessary. The objective of this study was to determine the concentration of Benzine Amino Purine (BAP) and Kinetin for somatic embryo maturation and to observe the maturation stage for somatic embryo of sugarcane cultivar PS 864. Maturation of the nodular callus was conducted by addition of Kinetin (0, 1, 3, and 5 mg/L) and BAP (0 and 5 mg/L) in solid and liquid medium. The medium was optimized using glutamine. The medium for somatic embryo germination used full and half strength Murashige and Skoog medium (MS) supplemented with 20 g/L sucrose and amino acid or growth regulator (BAP and NAA). Nodular callus well grew in solid medium, whereas callus become browning and not existence of somatic embryo maturation in liquid medium. The highest number of globular embryos (38 embryos) was produced from MS medium supplemented with 1 or 3 mg/L Kinetin combined with 5 mg/L BAP. The highest number of scutellum (21 embryos) and coleoptile (19 embryos) resulted from 3 mg/L Kinetin. The MS medium with full strength was added with 100 mg/L glutamine that was the best germination medium. This medium resulted the highest percentage of somatic embryo (73.29%) forming bipolar structure, and the largest number of leaves (4.58). Histological analysis showed that somatic embryos of sugarcane emerged from many cells through the budding process and also initiated from one cell.
KARAKTERISTIK GENETIK Kappaphycus alvarezii SEHAT DAN TERINFEKSI PENYAKIT ICE-ICE DENGAN METODE Amplified Fragment Length Polymorphism (AFLP) Emma Suryati; Lida Puspaningtyas; Utut Widyastuti; Suharsono Suharsono
Jurnal Riset Akuakultur Vol 8, No 1 (2013): (April 2013)
Publisher : Pusat Riset Perikanan, Badan Riset dan Sumber Daya Manusia Kelautan dan Perikanan

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (132.895 KB) | DOI: 10.15578/jra.8.1.2013.21-31

Abstract

Infeksi penyakit ice-ice pada Kappaphycus alvarezii seringkali menyebabkan penurunan produksi yang sangat signifikan. K. alvarezii merupakan alga merah penghasil karaginan yang memiliki nilai ekonomi tinggi dan banyak dimanfaatkan dalam berbagai industri, seperti farmasi, makanan, stabilizer, dan kosmetik. Perbaikan genetik sangat diperlukan untuk meningkatkan produksi. Penelitian ini bertujuan untuk mengetahui karakteristik kemiripan genetik K. alvarezii sehat dan terinfeksi penyakit dari Balai Penelitian dan Pengembangan Budidaya Air Payau (BPPBAP), Maros dengan metode Amplified Fragment Length Polymorphism (AFLP). Pada penelitian ini juga dianalisis K. alvarezii asal Bone (BNE), Gorontalo (GRL), Tambalang (TMB), dan Kendari (KND) sebagai kontrol rumput laut sehat. Metode AFLP menggunakan enzim restriksi Psti dan Mset, preamplifikasi dan amplifikasi selektif diawali dengan isolsi DNA, uji genimoc DNA, restriksi dan ligasi. Hasil yang diperoleh menunjukkan penggunaan marker AFLP dengan primer forward P11 dan primer reverse M48, M49 dan M50 terhadap K. alvarezii yang berasal dari Takalar (TKL), dan Mataram (MTR), tanpa infeksi (sehat) dan terinfeksi penyakit Takalar ice (TKL+), Mataram ice (MTR+), serta K. alvarezii kontrol (BNE), (GRL), (TMB), dan (KND) menghasilkan 519 fragmen dalam 122 lokus dengan ukuran 50 - ~370 pb. Kemiripan genetik K. alvarezii yang terinfeksi penyakit ice-ice lebih rendah jika dibandingkan dengan yang sehat. Kemiripan genetik K. alvarezii dari Takalar sehat (TKL) dan terinfeksi ice-ice (TKL+) adalah 0,8176 dan MTR-MTR+ adalah 0,8033.
Co-Authors AKIHO YOKOTA Alex Hartana Andi Parenrengi Aris Tjahjoleksono Cici Indriani Dalimunthe Desriani Desriani Didy Sopandie Emma Suryati Fitri Damayanti Fuad Al Ahwani Hamim Hamim Iif Rahmat Fauzi Ika Mariska KINYA AKASHI Lida Puspaningtyas Muhammad Jusuf Muhammad Jusuf Radite Tistama RADITE TISTAMA Radite Tistama Ratih Dewi Hastuti Rochadi Abdulhadi Seagames Waluyo Sri Rahayu Sustiprijatno Sustiprijatno Teguh Juliyanto Ulia Fajriah Utut Widyastuti UTUT WIDYASTUTI Utut Widyastuti Utut Widyastuti Utut Widyastuti Utut Widyastuti YanRiska Venata Sembiring Yohana C Sulistyaningsih