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I Made Tasma
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Indonesia
Agriculture, Biological Sciences & Forestry

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Genetic Diversity Analysis of 14 Potato Genotypes Based on Morphological Characters and SSR Markers Kristianto Nugroho; Rerenstradika T. Terriyana; Kusmana Kusmana; Puji Lestari; I Made Tasma
Jurnal AgroBiogen Vol 15, No 2 (2019): December
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v15n2.2019.p53-64

Abstract

Potato is one of important carbohydrate sources used as an alternative crop in Indonesia. The challenges in national potato breeding program included low productivity, less tolerance to environmental stresses, and narrow genetic diversity. The purpose of this study was to analyze genetic diversity of 14 potato genotypes based on morphological characters and SSR markers newly developed from genome sequences of Indonesian potato genotypes. Principal component analysis of morphological data was done using program XLSTAT. DNA of 14 potato genotypes were assayed using 22 SSR markers. Phylogenetic tree was constructed using program NTSYS version 2.1. The PCA showed that leaf shape, leaf color, tuber shape, tuber skin color, and tuber color contributed most to the total diversity. SSR polymorphism analysis demonstrated that as many as 196 alleles were detected in this study. The average allele number was 8.9 ranged from 5 to 13 alleles per locus. The average major allele frequencies was 22% ranged from 14 to 43%. Gene diversity ranged from 0.70 to 0.92 with the average of 0.86, meanwhile the heterozigosity observed ranged from 0 to 0.71 with the average of 0.05. Phylogenetic analysis generated two main clusters in the coefficient of similarity 0.77. The first cluster consisted of three genotypes while the second cluster consisted of eleven genotypes. The new developed SSR markers used in this study were able to differentiate potato accessions having similar morphological characters but were different genetically. The results of this study should be useful in assessing genetic materials in potato cultivar development program.
Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and Musa acuminata ssp. microcarpa Based on SSR Markers Dea Rosalia; Puji Lestari; Andy Soegianto; Darmawan Saptadi; Agus Sutanto; Kristianto Nugroho; Rerenstradika T. Terryana; I Made Tasma; Ika Roostika
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p17-24

Abstract

Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC >0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.
Co-Authors Agus Sutanto Andy Soegianto Darmawan Saptadi Dea Rosalia Ika Roostika Kristianto Nugroho Kusmana Kusmana Puji Lestari Rerenstradika T. Terriyana Rerenstradika T. Terryana