Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal AgroBiogen
Kurniawan R. Trijatmiko
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
Agriculture, Biological Sciences & Forestry

Published : 4 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 4 Documents
Search

 1 
Identifikasi Perubahan Karakter Agronomis Padi Transgenik Penanda Aktivasi cv. Asemandi Generasi T1 Atmitri Sisharmini; Aniversari Apriana; Diah Nurmaliki; Tri Joko Santoso; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p107-116

Abstract

The activation-tagged populations of transgenic rice cv.Asemandi have been developed by introducing construct ofactivation taq Ac/Ds into rice genom of cv. Asemandi. The T1transgenic rice populations cv. Asemandi containingconstruct of activation taq have been obtained and neededto be characterized. This study aimed to identify the Bastaherbicide resistant plants, transgenic plants containing hptand bar genes, and changes in agronomic traits. Bastaresistant plant was identified by treating leaf with bastasolution. Hpt and bar genes were detected by PCR usingspecific primers. Phenotype characters were identified byobserving and measuring their agronomic parameters. Thestudy results showed that out of 315 rice transgenic cv.Asemandi T1 treated with Basta solution, 176 (55.87%) plantswere indicated to be resistant to Basta. The results of PCRanalysis revealed that eight rice transgenic cv. Asemanditested contained both hpt and bar genes. In general,compared to the nontransgenic plants, there were changesin several agronomic parameters of T1 transgenic plants cv.Asemandi, including plant height, days to flowering, days toharvesting, periods of grain filling, and weight of 100 grains.Correlation analysis showed that there was no correlationbetween days of harvesting to weight of 100 grains intransgenic rice, but there was correlation in nontransgenicrice. Transgenic rice plants cv. Asemandi with changes inthe agronomic characters will be useful for further study,such as to analyze the function of the genes.
Deteksi Gen HptII dan Keragaan Agronomis pada Populasi BC1F1 Tanaman Padi Transgenik Budi Santosa; Kurniawan R. Trijatmiko; Tri J. Santoso
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p117-124

Abstract

Rice varieties tolerant to drought stress are needed tostabilize rice production under drought stress condition. Wedeveloped transgenic rice cv. Nipponbare carrying hptIIgene that might also contain OsDREB1A gene. OsDREB1Agene responsible to drought tolerance trait need to betransferred into cultivated rice in order to obtain new localrice variety tolerant to drought stress. The aims of thisresearch were to detect the presence of hptII gene in the F1and BC1F1 transgenic rice and to observe the agronomicperformace of those populations and their plant physiology.F1 population was developed by crossing transgenicNipponbare, as donor parent, with Batutegi, Code, Ciherang,and Konawe genotypes, as recipient parents. BC1F1population was developed by backcrossing F1 transgenicline with recipient parents, respectively. The presence ofhptII gene was analyzed by PCR using a pair of primers forhptII. The observation of agronomic performance wascarried out in the green house, meanwhile the observationof stomata was done using microscope. The result of PCRanalysis showed that BC1F1 Batutegi trans, BC1F1 Code trans,BC1F1 Konawe trans1, BC1F1 Konawe trans3, dan BC1F1Konawe trans4 were detected carrying the hptII gene.Agronomic data showed that BC1F1 transgenic rice linesyielded panicles, filled grains, and total grains higher thanthose of recipent parents. Comparing to the recipientparents, BC1F1 Konawe trans1 and BC1F1 Konawe trans3 hadless stomata on the lower side of the leaf, but had morestomata on the upper side of the leaf.
Identifikasi Galur dan Gen-gen Terkait Toleran Kekeringan pada Padi Transgenik cv. T309 yang Mengandung Vektor Penanda Aktivasi Tri Joko Santoso; Aniversari Apriana; Atmitri Sisharmini; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p97-106

Abstract

Activation tagging is an efficient tool forfunctional analysis of the rice genes. We have developed anumber of transgenic rice lines (Oryza sativa L. ssp.japonica cv. Taipei 309) containing activation tagging vector.However, the phenotypes and genotypes of these lines, inrelation to the drought stress, have not been analyzed. Theobjectives of this research were to identify transgenic ricelines that showed tolerance to the drought stress and toidentify the genes that may be associated with the droughtstress. The drought stress tolerance in transgenic rice lineswas identified by testing their tolerance to the drought stressand also by detecting the presence of bar and nptII genes.The result showed that 56 out of 59 rice lines were resistantto Basta herbicide and three of them showed tolerance todrought stress, namely PA.T-1.2, PA.T-4.1, and PA.T-5.1 lines.PCR analysis showed that PA.T-1.2 and PA.T-4.1 containedboth hptII and bar genes, while the PA.T-5.1 line containedbar gene only. Thermal Asymetric Interlaced-PCR (TAILPCR)analysis showed that two genes may be asssociatedwith the drought stress tolerance. Those genes areOSJNBa0004120.14 that produces uridylate putative kinaseand OsPPCK2L that produces phosphoenolpyruvatecarboxylase kinase.
Survei Polimorfisme Tetua untuk Pengembangan Panel CSSL Padi (Oryza sativa L.) dan Identifikasi Tanaman F1 Mariana Susilowati; Panjisakti Basunanda; Wening Enggraini; Ma'sumah Ma'sumah; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 10, No 3 (2014): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n3.2014.p85-92

Abstract

Raising yield potential of modernindica varieties is essential to meet the increased demand ofrice production. This is due to increased human population,threats of climate change and degradation of agriculturalresources. The use of chromosome segment substitutionlines (CSSL) is more effective for identification of genesthose are useful for improvement of yield potential. The aimsof this study were to observe the morphological traitdifferences between recipient parent (var. Ciherang) andthree candidates of donor parent (var. Fatmawati and newplant type lines, i.e. B12743 and B11143D), to identifypolymorphic SSR markers among them and to verify F1individuals. Ciherang and B11143D showed significantdifferences on flowering time, plant height, flag leaf area,tiller number, productive tiller number, panicle length,spikelet number per panicle and 1,000 grain weight. The rateof SSR marker polymorphisms between Ciherang andB11143D was the highest, where 155 of 513 markers (30.2%)were polymorphic. Marker genotyping using threepolymorphic markers showed that 26 of 27 plants resultedfrom the cross of Ciherang х B11143D were F1. These F1plants could become the basis of CSSL panel that facilitatethe mapping of genes responsible for increasing the yieldpotential.
Co-Authors Aniversari Apriana Atmitri Sisharmini Budi Santosa Diah Nurmaliki Ma'sumah Ma'sumah Mariana Susilowati Panjisakti Basunanda Tri J. Santoso Tri Joko Santoso Wening Enggraini