Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal AgroBiogen
Tri Joko Santoso
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jl. Tentara Pelajar 3A, Bogor 16111 Telp. (0251) 8337975; Faks. (0251) 8338820
Agriculture, Biological Sciences & Forestry

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Identifikasi Perubahan Karakter Agronomis Padi Transgenik Penanda Aktivasi cv. Asemandi Generasi T1 Atmitri Sisharmini; Aniversari Apriana; Diah Nurmaliki; Tri Joko Santoso; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p107-116

Abstract

The activation-tagged populations of transgenic rice cv.Asemandi have been developed by introducing construct ofactivation taq Ac/Ds into rice genom of cv. Asemandi. The T1transgenic rice populations cv. Asemandi containingconstruct of activation taq have been obtained and neededto be characterized. This study aimed to identify the Bastaherbicide resistant plants, transgenic plants containing hptand bar genes, and changes in agronomic traits. Bastaresistant plant was identified by treating leaf with bastasolution. Hpt and bar genes were detected by PCR usingspecific primers. Phenotype characters were identified byobserving and measuring their agronomic parameters. Thestudy results showed that out of 315 rice transgenic cv.Asemandi T1 treated with Basta solution, 176 (55.87%) plantswere indicated to be resistant to Basta. The results of PCRanalysis revealed that eight rice transgenic cv. Asemanditested contained both hpt and bar genes. In general,compared to the nontransgenic plants, there were changesin several agronomic parameters of T1 transgenic plants cv.Asemandi, including plant height, days to flowering, days toharvesting, periods of grain filling, and weight of 100 grains.Correlation analysis showed that there was no correlationbetween days of harvesting to weight of 100 grains intransgenic rice, but there was correlation in nontransgenicrice. Transgenic rice plants cv. Asemandi with changes inthe agronomic characters will be useful for further study,such as to analyze the function of the genes.
Konstruksi Kandidat Gen AV1 Begomovirus pada pBI121 dan Introduksinya ke dalam Tembakau Menggunakan Vektor Agrobacterium tumefaciens Tri Joko Santoso; Muhammad Herman; Sri H Hidayat; Hajrial Aswidinnoor; Sudarsono Sudarsono
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p9-18

Abstract

Construction of Begomovirus AV1 Gene Candidate intopBI121 and Its Introduction into Tobacco by usingAgrobacterium tumefaciens Vector. Tri J. Santoso,Muhammad Herman, Sri H. Hidayat, HajrialAswidinnoor, and Sudarsono. Infection of Begomovirushas caused leaf curl disease in tomato. This infection hassignificantly impact on yield losses of tomato production.Recently, in Indonesia there was no effectively way tocontrol this disease. The use of resistant tomato variety isone of strategies to control this virus. Genetic engineeringtechnology gives an opportunity to develop the transgenictomato resistant to Begomovirus through pathogen derivedresistance (PDR) approach. The objectives of this studywere to construct the Begomovirus AV1 candidate gene inthe pBI121 and to introduce the construct into tobacco plantgenome through Agrobacterium tumefaciens vector. A seriesactivites in gene construct have been conducted includePCR amplification of AV1 gene using a pair of specificprimer, cloning the gene into pGEM-T easy, transformation ofthe clone into Escherichia coli DH5α competent cell,construct the gene into pBI121, and transform the constructinto A. tumefaciens. Leaf segments of in vitro tobacco plantwere transformed by co-cultivation with A. tumefacienscontaining ToLCV-AV1 construct. In the research activitiy,Indonesian Begomovirus AV1 gene was successfullyamplified and inserted in expression vector plasmid pBI121.Tobacco transformants carrying kanamycin-resistant gene(nptII gene) were regenerated and established in theglasshouse. Those transformant plants are expectedcontaining the AV1 gene.
Identifikasi Galur dan Gen-gen Terkait Toleran Kekeringan pada Padi Transgenik cv. T309 yang Mengandung Vektor Penanda Aktivasi Tri Joko Santoso; Aniversari Apriana; Atmitri Sisharmini; Kurniawan R. Trijatmiko
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p97-106

Abstract

Activation tagging is an efficient tool forfunctional analysis of the rice genes. We have developed anumber of transgenic rice lines (Oryza sativa L. ssp.japonica cv. Taipei 309) containing activation tagging vector.However, the phenotypes and genotypes of these lines, inrelation to the drought stress, have not been analyzed. Theobjectives of this research were to identify transgenic ricelines that showed tolerance to the drought stress and toidentify the genes that may be associated with the droughtstress. The drought stress tolerance in transgenic rice lineswas identified by testing their tolerance to the drought stressand also by detecting the presence of bar and nptII genes.The result showed that 56 out of 59 rice lines were resistantto Basta herbicide and three of them showed tolerance todrought stress, namely PA.T-1.2, PA.T-4.1, and PA.T-5.1 lines.PCR analysis showed that PA.T-1.2 and PA.T-4.1 containedboth hptII and bar genes, while the PA.T-5.1 line containedbar gene only. Thermal Asymetric Interlaced-PCR (TAILPCR)analysis showed that two genes may be asssociatedwith the drought stress tolerance. Those genes areOSJNBa0004120.14 that produces uridylate putative kinaseand OsPPCK2L that produces phosphoenolpyruvatecarboxylase kinase.
Co-Authors Aniversari Apriana Atmitri Sisharmini Diah Nurmaliki HAJRIAL ASWIDINNOOR Kurniawan R. Trijatmiko Muhammad Herman Sri H Hidayat Sudarsono Sudarsono