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Mia Kosmiatin
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry

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Perkecambahan dan Perbanyakan Gaharu secara In Vitro Mia Kosmiatin; Ali Husni; Ika Mariska
Jurnal AgroBiogen Vol 1, No 2 (2005): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n2.2005.p62-67

Abstract

Agarwood (Aquilaria malaccensis Lank) is one of the forest wood that are continously exploited. Currently, the Indonesian export of agarwood is decreasing because its population is endangered by excessive logging. Agarwood propagations need technology for reproduction of agarwood seedlings and their fungal inoculum. In vitro technique for germination of recalsitrant seeds and micropropagation are technologies that can be used for propagation of agarwood seedlings. An experiment was done to develop techniques for in vitro germination and micropropagation of agarwood. The in vitro germination was done using two different techniques. Firstly, sterile seeds were germinated on an MS medium + 50 mg/l PVP, 50 mg/l GA, and 1 mg/l BA or kinetin. Secondly, sterile seeds were germinated on basal medium of MS, 1/2 MS medium, MS medium without vitamins, as well as on MS medium without pyridoxine, nicotinic acid and WPM. Shoot initiations and multiplications were done on MS and 1/2 MS media containing 1, 3, or 5 mg/l BA. The explants used were cotyledone nodes, terminal shoots, single node with leaf, and sinle node without leaf. The results showed that the seed germination rate on the different media ranged from 7,14 to 50%. The seed germination rate on the MS medium without vitamis was the highest. The best explants for shoot induction and multiplication was single node with leaf which was cultured on MS + 1 mg/l BA.
Seleksi In Vitro Tanaman Lada untuk Ketahanan terhadap Penyakit Busuk Pangkal Batang Ali Husni; Mia Kosmiatin
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p13-19

Abstract

Root rot caused by Phytopthora capsici is one of the most important diseases of pepper, it can decrease yield up to 52%. Planting resistant plants is an efficient way to control the disease. In vitro selection is a method that can be utilized for selection of resistant plants. The objective of the study was to obtain pepper plants resistant to root rot disease through in vitro selection. The study consisted of four experiments, i.e., (1) induction of embryogenic calli, (2) production of filtrate of P. capsici, (3) in vitro selection and shoots regeneration, and (4) root induction. The results showed that leaf tissue was the best explants for production of embryogenic calli in a medium of Gamborg (macro nutrient) + MS (micro nutrients and vitamins) with 2.4-D 0.1 or 0.5 mg/l. The best filtrate of P. capsici culture used for the selection was that from the 5 day-old inoculum in the V8 medium. In general, addition of P. capsici culture filtrate into the regeneration media influenced the percentage of live calli. The addition of 50% and 75% of P. capsici filtrate was enabling to screening for adaptive calli based on brownish-yellow or yellowish-brown color. These calli produced 32 shoots in the regeneration media without the P. capsici filtrate. Root induction was successfully performed in the MS medium with 0.01 mg/l of NAA.
Co-Authors Ali Husni Ika Mariska