Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal AgroBiogen
Dwinita Wikan Utami
Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian, Jalan Tentara Pelajar 3A, Bogor 16111
Agriculture, Biological Sciences & Forestry

Published : 3 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 3 Documents
Search

 1 
Gen Pengendali Sifat Ketahanan Penyakit Blas (Pyricularia grisea Sacc.) pada Spesies Padi Liar Oryza rufipogon Griff. dan Padi Budi Daya IR64 Dwinita Wikan Utami; Sugiono Moeljopawiro; Hajrial Aswidinnoor; Asep Setiawan; Ida Hanarida
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p1-6

Abstract

Improvement of rice for durable resistance rice blast is difficult due to the complexity of the inheritance of resistance. As study was conducted to analyze blast resistance in rice using two different approaches, i.e., blast QTL mapping and comparison of resistance spectrum and genetic control. The blast QTL mapping was done using an interspesific population originated from backcrossing between a wild rice, Oryza rufipogon, and a cultivated rice, IR64. Comparison of resistance spectrum and genetic control was based on phenotypic reactions. Results of the experiment showed that based on the blast QTL mapping, genes Pirf2-1(t) and Pir2-3(t) were mapped on chromosome 2. Gene Pirf2-1(t) was isolated from chromosome 2 of O. rufipogon and coding for resistance to P. grisea race 001, while gene Pir2-3(t), which was isolated from rice cultivar IR64, was coding for resistance to P. grisea race 173. Based on the resistance spectrum, O. rufipogon has a non-race specific resistance. Gene Pirf2-1(t) on O. rufipogon contributed a dominant mode of resistance to blast which was affected by a duplicate epistasis. The other gene, Pir2-3(t) contributed an additive mode of resistance which was affected by a complementary epistasis.
Manfaat Sekuen Genom Lengkap dalam Identifikasi Gen: Peranan Kelompok Gen Actin-myosin dalam Sistem Pertahanan Tanaman Reflinur Reflinur; Dwinita Wikan Utami
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p38-44

Abstract

Complete genome sequencing of Arabidopsis thaliana and rice (Oryza sativa) were accomplished in 2000 and 2004, respectively. The availability of high quality genome sequences of A. thaliana and rice amenable for identification and understanding of the structure and functional genes in the plant genome. One of the genes family that have been investigated is the actin-myosin genes. This genes family contributes to signalling process of the plant defence mechanism. This paper focuses on phylogenetic characterization and activation of actin-myosin genes family with emphasis on involvement on the plant defence mechanism.
Struktur Populasi Trichogrammatoidea armigera, Parasitoid Telur Helicoverpa armigera, Berdasarkan Analisis RAPD-PCR Bahagiawati Amir Husin; Damayanti Buchari; Nurindah Nurindah; Habib Rizjaani; Dwinita Wikan Utami; B. Sahari; A. Sari
Jurnal AgroBiogen Vol 2, No 2 (2006): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n2.2006.p52-59

Abstract

Population Structure of Trichogrammatoidea armigera,Egg Parasitoid of Helicoverpa armigera Based on RAPDPCRAnalysis. Bahagiawati, Damayanti Buchari, Nurindah,H. Rizjaani, Dwinita W. Utami, B. Sahari, and A.Sari. Genetic structures of Trichogrammatoidea armigera(Hymenoptera: Trichogrammatidae), the egg parasitoid ofHelicoverpa armigera (Lepidoptera: Noctuidae) were studied.Egg masses of H. armigera were collected from fields ofseveral locations in West Java and East Java with differentdistances among them and two distinct cultural practices,i.e., monoculture and polyculture. Genetic relationshipsamong T. armigera populations that emerged from the collectedH. armigera eggs were analysed by the RAPD-PCRtechnique using four oligonucleotide primers. The fourprimers revealed 55 presumptive polymorphic loci that wereused to estimate the population structures. The estimatedvalues of Fixation Index (Fst) was 0.16, indicating that therewas a division of the populations into subpopulations. ThisFst value implied the present of reproductive isolationamong the populations that might be due to their lowmigration rate (1.3 insect per generation). This low migrationrate indicated the present of low level of gene flow amongthe populations. A dendrogram resulted from the NTSYSanalysis indicated that the West Java and East Java populationsof the egg parasitoid had quite wide genetic distances,while within each of the populations there was a subdivisionof minor populations. This finding has an important implicationon the program to release Trichogramma spp. as a biologicalcontrol agent. The release of the parasitoid cannot bedone randomly, because if we pick up a minor population,the starter or the released population will mate with thelocal population and multiply, thus the inundation will fail tocontrol the target pest.
Co-Authors A. Sari B. Sahari Bahagiawati Amir Husin Damayanti Buchari Habib Rizjaani HAJRIAL ASWIDINNOOR Ida Hanarida Nurindah Nurindah Reflinur Reflinur Setiawan, Asep S Sugiono Moeljopawiro