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Karyono -
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Health Professions Immunology & microbiology Nursing Public Health

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RESPONS PERTUMBUHAN, KADAR PROTEIN DAN AKTIVITAS TRIPTOFAN DEKARBOKSILASE AGREGAT SEL Catharanthus roseus (L) G. Don YANG DIBERI PREKURSOR TRIPTOFAN Dingse Pandiangan -; Wenny Tilaar -; Karyono -; Rizkita Rahmi Esyanti -; Anas Subarnas -
Bionatura Vol 13, No 1 (2011): Bionatura Maret 2011
Publisher : Direktorat Sumber Daya Akademik dan Perpustakaan

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Abstract

Salah satu cara untuk meningkatkan kandungan metabolit sekunder dalamkultur jaringan adalah dengan penambahan prazat (prekursor). Oleh karena itutelah dilakukan penelitian mengenai penambahan triptofan sebagai prekursorterhadap pertumbuhan, kadar protein dan aktivitas Triptofan Dekarboksilase(TDC) pada kultur agregat sel Catharanthus roseus (L) G.Don. Penelitian inibertujuan untuk meningkatkan kandungan katarantin dalam kultur agregat selCatharanthus roseus (L) G.Don yang didukung dengan pertumbuhan yangoptimum. Penelitian dilakukan secara eksperimental di laboratorium. Konsentrasiprekursor triptofan digunakan dari 50-250 mg.L-1. Pertumbuhan ditentukandengan penimbangan berat basah dan berat kering serta pengamatan perubahansecara morfologis. Kadar protein ditentukan dengan spektrofotometer. AktivitasTDC ditentukan dengan spektrofluorometer. Pertumbuhan maksimum kulturagregat sel terjadi pada perlakuan triptofan 150 mg.L-1 pada Erlenmeyer.Penambahan triptofan dapat menginduksi sintesis protein dan meningkatkanaktivitas TDC pada kultur agregat sel C.roseus. Kandungan protein dan aktivitasTDC tertinggi terjadi pada perlakuan 250 mg.L-1 setelah 10-14 hari kultur.Perlakuan triptofan menimbulkan pengaruh yang berbeda terhadap kandunganprotein, aktivitas TDC, pada kultur Erlenmeyer. Kesimpulannya adalah perlakuanprekursor triptofan dapat meningkatkan kadar protein dan aktivitas TDC yangdiberi prekursor triptofan dan optimum pertumbuhan agregat selnya pada triptofan150 mg.L-1 setelah 14 hari kultur.Kata Kunci: Triptofan, agregat sel, Catharanthus roseus, kadar protein, aktivitasTDC
FAKTOR-FAKTOR YANG MEMPENGARUHI KEJADIAN PASIEN DIARE PADA ANAK DI RSUD MAJENANG KABUPATEN CILACAP TAHUN 2008 Karyono -; Basirun -; Cahyu Septiwi
Jurnal Ilmiah Kesehatan Keperawatan Vol 5, No 1 (2009): JURNAL ILMIAH KESEHATAN KEPERAWATAN
Publisher : LPPM UNIVERSITAS MUHAMMADIYAH GOMBONG

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Abstract

This research aim to know the factors influencing diarrhea occurrence in RSUD Majenang year 2008 and to know the most dominant factor of  its influence to diarrhea occurrence. This Research type is observational research by design of research is cross sectional approach. Population in this research is family member who suffered from diarrhea at Majeneng General Hospital. Sample was taken by purposive sampling method. The number of respondents was 83. Statistical analysis used is Multiple Regression Logistics to know the dominant factor from a number of variable independent that is infection, personal hygiene and environmental sanities to diarrhea occurrence. Result of research shown that there are significance relation between infection factor to diarrhea occurrence. Pursuant to analyze result of logistics regression known that the value significance ( sig.) for the variable of infection  equal to 0,005 smaller than a ( 0,05). There are significance relation between factor of personal hygiene with the diarrhea occurrence. Pursuant to result analyses of logistics regression known that the significance value ( sig.) for the variable of personal hygiene equal to 0,043 smaller than a  ( 0,05). There are significance relation between environmental sanities factor with diarrhea occurrence. Result of analysis of logistics regression obtained that significance value ( sig.) for the variable of environmental sanitize equal to 0,021 smaller than a ( 0,05). And pursuant to result analyzes the logistics regression known that the highest wald value is infection variable that is equal to 7,850 with the significance value ( sig.) equal to 0,005 smaller thana ( 0,05) so that most dominant factor its influence to diarrhea occurrence infection factor. The Research conclusion is that there are significant relation between infection factor, personal Hygiene and environmental sanitize with the diarrhea occurrence of most dominant factor and its influence to diarrhea occurrence is infection factor   Keywords: infection, personal hygiene, environmental sanities, diarrhea disease
Co-Authors Anas Subarnas Basirun - Cahyu Septiwi Dingse Pandiangan - Rizkita Rahmi Esyanti - Wenny Tilaar -