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All Journal Jurnal Sain Veteriner Buletin Peternakan Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati Jurnal Penelitian Pascapanen Pertanian Jurnal Ilmu Ternak Veteriner WARTAZOA Indonesian Bulletin of Animal and Veterinary Sciences
Widodo Suwito
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Veterinary Other

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Teknologi Penanganan Susu Yang Baik Dengan Mencermati Profil Mikroba Susu Sapi Di Berbagai Daerah Widodo Suwito; nFN Andriani
Jurnal Penelitian Pascapanen Pertanian Vol 9, No 1 (2012): Jurnal Penelitian Pascapanen Pertanian
Publisher : Balai Besar Penelitian dan Pengembangan Pascapanen Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jpasca.v9n1.2012.35-44

Abstract

Susu merupakan salah satu pangan asal ternak yang memiliki kandungan nutrisi tinggi sehingga menyebabkan mikroba mudah tumbuh dan berkembang. Tujuan penelitian ini adalah untuk mendapatkan cara penanganan susu yang baik dengan mengetahui kualitas mikrobiologi susu dari peternakan di kabupaten Bogor, Sukabumi dan Cianjur berdasarkan Standar Nasional Indonesia (SNI) No 01-6366-2000 tentang persyaratan susu segar. Sebanyak 351 sampel susu, 19 sampel air dan 24 sampel swab milk can dikumpulkan dari peternakan Kabupaten Bogor, Sukabumi dan Cianjur. Sampel susu dan air diperiksa terhadap jumlah Coliform dan E. coli dengan metode Most Probable Number (MPN). Total Plate Count (TPC) dan jumlah Staphylococcus aureus dalam susu dihitung dengan metode Association of Official Analytical Chemist (AOAC). Deteksi Salmonella sp dalam susu, air dan swab milk can dengan metode isolasi dan identifikasi secara biokimawi. Hasil penelitian ini menunjukkan bahwa susu dari peternakan di kabupaten Bogor, Sukabumi dan Cianjur sebanyak 79,48% mendapatkan dengan cara penanganan yang baik serta memenuhi SNI No 01-6366-2000.
Verotoxigenic Escherichia coli (VTEC) isolated from cow milk Widodo Suwito
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (183.713 KB) | DOI: 10.14334/jitv.v14i3.346

Abstract

Verotoxigenic Escherichia coli (VTEC) strains are responsible for serious human illnesses. These strains are commonly found in milk. The aim of this study was to determine the occurrence of verotoxigenic E. coli in milk. A total of 351 milk samples, were collected from dairy farms in Bogor, Sukabumi and Cianjur. These samples were analyzed for VTEC using biochemical, serological and vero cell cytotoxicity assays. VTEC O157:H7 isolates were found in milk collected from dairy herds in Bogor and Sukabumi at rates 0.47% of 214 samples, 1.10% of 91 samples respectively, and none in Cianjur. Hemolytic E. coli isolates were found in 0.94% of 214 milk samples from Bogor, 2.2% of 91 milk samples from Sukabumi and none from Cianjur. From E. coli isolates, 53 isolates (67.95%) were verotoxigenic, consisted of: two E. coli O157:H7 isolates and 51 non O157:H7 isolates.Therefore this study showed the occurrence of VTEC in milk samples from dairy farms in Bogor, Sukabumi and Cianjur. Key words: Milk, E. coli (VTEC) O157:H7, Verotoxigenic
Impacts of Verotoxigenic and Enterohaemorrhagic Escherichia Coli (Vtec and Ehec) on Animal, Human and Food Widodo Suwito
WARTAZOA, Indonesian Bulletin of Animal and Veterinary Sciences Vol 19, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.554 KB) | DOI: 10.14334/wartazoa.v19i2.922

Abstract

Verotoxigenic and enterohaemorrhagic Escherichia coli, VTEC and EHEC are strains that produce a toxin on the Vero cells in vitro, therefore the toxin called verotoxin. Strain VTEC and EHEC have been isolated from human and various animal species, mainly ruminants and pigs. The prevalence of VTEC in cattle is 35%, milk 10%, cheese 1.5%. In beef meat, pork, poultry, goat and sheep is 3.7, 1.5, 1.5, 2 and 2.5%, respectively. In contrast, the human prevalence in United States, Spain and France 5 – 10, 2.5 and 3.4%, respectively. In Indonesia, nine cases of VTEC were reported by Ciptomangunkusumo Hospital and four out of nine cases were died. The verotoxin also named Shiga toxins (Stx), is active in vivo as a pathogen on the endothelial cells of the blood vessels of the gastro intestinal mucosa, kidneys, brain and other tissues of human and piglets. Verotoxin in human, ruminants and piglets causes bloody diarrhea so with ruminants. In the adult ruminants, verotoxin results in clinical signs and they act as asymptomatic carriers of VTEC and EHEC strains but in human, it produced haemorrhagic colitis (HC), haemolytic uremic syndrome (HUS) and thrombocytopenia purpura (TPP). Infection in human by the most famous EHEC strain belongs to the O157:H7 serotype, through faecal contamination or environment of either food of animal origin, or other foodstuffs (fruits and vegetables). Infection from EHEC strains could be prevented by avoiding food of animal origin and unpasteurized milk, and by taking care of food hygiene for comsumption.   Key words: verotoxin, enterohaemorrhagic, VTEC, EHEC, Shiga toxin (stx), ruminant
DETEKSI Escherichia coli 0157:H7 DARI SUSU DAN DAGING MENGGUNAKAN SERUM KEBAL MONOSPESIFIK Widodo Suwito
Jurnal Sain Veteriner Vol 28, No 2 (2010): DESEMBER
Publisher : Fakultas Kedokteran Hewan Universitas Gadjah Mada bekerjasama dengan PB PDHI

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2951.202 KB) | DOI: 10.22146/jsv.295

Abstract

Escherichia coli 0157:H7 merupakan salah satu bakteri patogen penyebab food borne disease pada manusia. Deteksi E.coli 0157:H7 pada serum keba] monospesifik berdasarkan somatik antigen (0) dan flagella(H). Tujuan dari penelitian ini untuk mendeteksi E.coli 0157:H7 menggunakan serum kebal monospesifik dengan cara absorbsi dan uji koaglutinasi. Serum kebal monospesifik diperoleh dengan cara menyuntikkan antigen E.coli 0] 57:H7 pada kelinci jenis New Zealand White. Serum dikoleksi dan diabsorbsi menggunakan antigen somatik E.coli heterolog sampai tidak terjadi reaksi agglutinasi. Hasil penelitian menunjukkan bahwa dengan serum kebal monospesifik dapat digunakan untuk menedeteksi E.coli 0 157:H7 dalam susu dan daging.Kata kunci: serum keba1, monospesifik, E.coli 0157:H7
Monitoring Salmonella sp dan Escherichia coli dalam Bahan Pakan Ternak (Salmonella sp and Escherichia coli Monitoring in the Animal Feed Ingredients) Widodo Suwito
Buletin Peternakan Vol 34, No 3 (2010): Buletin Peternakan Vol. 34 (3) Oktober 2010
Publisher : Faculty of Animal Science, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21059/buletinpeternak.v34i3.86

Abstract

Quality of livestock feed affects production and animal health. Fish and bone meal is animal feed ingredients that need to be monitored because highly contaminated with of Salmonella sp and E. coli. Salmonella sp and E. coli arepathogen causing diarrhea in cattle and humans. The aim of this study was to investigate both E. coli and Salmonella contamination animal feed ingredients. A total of 38 animal feed ingredients samples from a poultry farm in Sukabumiconsisting of 15 samples fish meal, bone meal 13 samples and corn 10 samples. These samples were analyzed for Salmonella sp and E. coli by isolation and identification using biochemical analysis. Salmonella Weltevreden wasisolated from fish meal at rates of 6% from the 15 samples. E. coli was isolated at rates of 6% from the 15 fish meal samples and 7% from 13 bone meal samples, while corn samples were free from Salmonella sp and E. coli. This studyshowed that bone meal and fish meal was contaminated slightly by Salmonella Weltevreden and E. coli.(Key words: Feed, Livestock, Salmonella sp, E. coli)
Determinasi Salmonella sp dan Ektoparasit dalam Pupuk Organik dari Kotoran Sapi Potong di Yogyakarta Widodo Suwito; Supriadi -; Erna Winarti; Utomo Bimo Bekti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 18, No 2 (2013): June 2013
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v18i2.388

Abstract

One of by products derived from beef cattle farm is manure. The manure can be processed to make organic fertilizer. Salmonella sp is bacterium of the manure origin and harmful for human. Currently, organic fertilizer is mostly used for vegetables and fruits, but the problem is people consume those vegetables. The aim of this study was to determine Salmonella sp and ectoparasite in organic fertilizer which were made from the manure of beef cattle farm in Yogyakarta. A total of 10 manure samples were collected the farms to make organic fertilizer. The manure was fermented with lactic acid bacteria (BAL) for one month. Before and after fermented, the manure was isolated and identified for Salmonella sp based on biochemical reactions and ectoparasite using native method. The study showed that there were no Salmonella sp and ectoparasite in the organic fertilizer made from the manure fermented for one month. Keywords: Organic fertilizer, manure, farm, Salmonella sp Abstrak Salah satu hasil sampingan dari peternakan sapi potong adalah kotoran ternak. Kotoran ternak dapat dimanfaatkan untuk dibuat pupuk organik. Salmonella sp merupakan bakteri yang berasal dari kotoran ternak dan dapat membahayakan kesehatan manusia. Saat ini pupuk organik banyak digunakan untuk tanaman sayuran dan buah-buahan, sedangkan sebagian masyarakat mengonsumsi sayuran dalam keadaan mentah. Tujuan dari penelitian ini adalah untuk mengetahui Salmonella sp dan ektoparasit dalam pupuk organik yang dibuat dari kotoran sapi pada peternakan sapi potong di Yogyakarta. Telah dikumpulkan sebanyak 10 sampel kotoran sapi yang akan dibuat pupuk organik dari peternakan sapi potong di Yogyakarta. Kotoran sapi difermentasi dengan bakteri asam laktat (BAL) selama satu bulan. Kotoran sapi sebelum dan sesudah difermentasi dilakukan isolasi dan identifikasi Salmonella sp berdasarkan reaksi biokimia dan ektoparasit dengan metode natif. Penelitian ini menunjukkan bahwa di dalam kotoran sapi yang dibuat pupuk organik dengan fermentasi selama satu bulan tidak ditemukan Salmonella sp. Kata kunci: Pupuk organik, rabuk, peternakan sapi, Salmonella sp
Co-Authors Erna Winarti nFN Andriani Supriadi - Utomo Bimo Bekti