Article Per Year (5 Year)
p-Index From 2020 - 2025
0
P-Index
This Author published in this journals
All Journal Jurnal Penelitian Tanaman Industri Jurnal Penelitian Tanaman Industri (Littri)
YANG NURYANI
Balai Penelitian Tanaman Rempah dan Obat
Agriculture, Biological Sciences & Forestry Industrial & Manufacturing Engineering

Published : 6 Documents Claim Missing Document
Claim Missing Document
Check
Articles

Found 6 Documents
Search

 1 
PENETAPAN KERAGAMAN GENETIK NILAM (Pogostemon sp.) HASIL FUSI PROTOPLAS DENGAN TEKNIK RAPD YANG NURYANI; OTIH ROSTIANA; CHEPPY SYUKUR
Jurnal Penelitian Tanaman Industri Vol 8, No 2 (2002): Juni, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n2.2002.39-44

Abstract

Keragaman genetik dan kckerabatan tanaman nilam hasil fusi protoplas antara Nilam Jawa (Girilaya) dan Nilam Aceh (Sidikalang dan TT 75) dianalisis dengan menggunakan penanda RAPD. Bahan tanaman yang digunakan dalam penelitian ini adalah 9 genotipa yang tcrdiri dari 3 tetua dan 6 tanaman hibrida somatik (9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 dan 9 II 10). Primer yang digunakan dalam analisis tcrdiri atas 5 primer acak yaitu OPD 03, OPD 20, OPH 09, OPH 19 dan Abi 117.17. DNA dickstraksi dengan metode OROZCO-CASTJLLO et al. (1994) yang sudah dimodifikasi. Konsentrasi DNA ditetapkan dengan metode sambrook el al. (1989) dengan pcrbandingan kuantifikasi spektrofotometrik. Koefisien kemiripan dan kckerabatan antar genotipa dianalisis dengan menggunakan program NTsys ver. 1.80 dan UPGMA untuk menentukan sidik gerombol dan dendogram. Hasil analisis menunjukkan bahwa koefisien kemiipan dari amplifikasi DNA dengan 5 primer acak dari ke- 9 genotipa berkisar antara 0.48-1.0. Berdasarkan niatrik jarak genetik, kescmbilan genotipa tanaman yang diuji membentuk 2 kelompok besar yaitu kelompok I, tetua Girilaya (Nilam Jawa) dan kelompok II tcrdiri dari tetua Sidikalang dan TT 75 (Nilam Aceh) serta hibrida somatik. Kelompok II, tcrbagi menjadi dua sub kelompok yaitu sub kelompok I (9 II 33 dan 9 II 7) dan sub kelompok II yang tcrdii dari sub-sub kelompok II-I (9 II 21, S, TT 75) dan sub-sub kelompok II-II (2 IV 8, 9 IV 14, 9 II 10).Kata kunci: Pogostemon sp., fusi protoplas, keragaman genetik, RAPD ABSTRACTS Assessment of genetic variability of patchoulli (Pogostemon sp.,) derived from protoplast fussion using RAPD Somatic hybrids of Pogostemon heyneaneus (cv. Girilaya) X P. cablin (cv. Sidikalang and TT 75) were tested for their genetic variability and relationship. The somatic hybrids tested were 9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 and 9 II 10. DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al. (1994) and quantified spectrophotometrically according to SAMBROOK el al. (1989). Five random primers, OPD 03. OPD 20, OPH 09. OPH 19 and Abi 117.17, were applied to amplify the extracted DNA. The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. Index of similarity among genotypes were calculated by using NTsys ver. 1.80 program. Then, cluster analyses to perform dendogram were achieved based on similarity estimates by using the Unweighted Pair-Group Method Arithmetic Average (UPGMA). Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0.48 to 1.0. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli (Sidikalang and TT 75), and the somatic hybrids. The second group was then classified into 2 minor groups. First group consisted of somatic hybrids nos. 9 II 33 and 9 II 7, while the second were classified into 2 groups which consisted nos. 9 II 21, S, TT 75 and nos. 2 IV 8, 9 IV 14 and 9 II 10.Key words : Pogostemon sp., protoplast fusion, genetic variability, RAPD
TANGGAP BEBERAPA KLON NILAM (Pogostemon cablin Benth.) TERHADAP NEMATODA PELUBANG AKAR (Radopholus simitis Cobb.) YANG NURYANI; CHEPPY SYUKUR; RITA HARNI; YELNI TITIS; REPIANYO REPIANYO; IKA MUSTIKA
Jurnal Penelitian Tanaman Industri Vol 5, No 3 (1999): Desember, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n3.1999.103-108

Abstract

Response of some patchouli clones (Pogostemon cablin Benth.) to burrowing nematode (Radopholus similis Cobb.)One of the seious poblems on patchouli is infection of plant parasitic nematode. Nine clones of Aceh patchouli (Pogostemon cablin Benth.) namely KJ (oiginated from tissue cultue) Meulaboh-2, Sidikalang, Lhokseumawe-2, Cisaroni . Cirateun, Tapak Tuan. Aceh Merah. Situak-2, and one clone of Java patchouli (P. heyneanus Benth.) namely Giilaya, were tested to infection of buowing nematode (Radopholus similis Cobb.) in the glasshouse of Research Institute for Spice and Medicinal Crops, Bogor fom July to November 1997. Each clone was inoculated with 500 of R. similis. An expeiment have been conducted to know the esponse of some patchouli cultivars against nematode. The expeiment was arranged in a Completely Randomized Design with thee replicates. The esults showed that the infection of R. similis reduced root weight greater than that of shoot weight, the colour of leaf became reddish or dark purple. Based on the development rate of nematode and level of damage, Giilaya appeared to be resistant, whereas Sidikalang, Cisaroni and Situak-2 clones wee slightly susceptible. KJ. Lhokseumawe-2, Cirateun. Tapak Tuan and Aceh Merah clones were susceptible, whereas Meulaboh-2 were highly susceptible against R. similis. Characteistic of Java clone that esistance and Aceh clone that shyhtly susceptible to R. similis may be useful for providing new vaieties with high content of oil and resistant to nematode.
TANGGAP TIGA VARIETAS LADA PERDU TERHADAP PUPUK ORGANIK YANG NURYANI; PASRIL WAHID
Jurnal Penelitian Tanaman Industri Vol 4, No 5 (1999): Januari, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v4n5.1999.135-139

Abstract

Response of three bushy black pepper vaieties to organic fetilizerResponse of three bushy black pepper varieties to organic fertilizer was studied in Sukamulya Research Insulation, rom February 1992 to August 1995. The plant materials used wee pepper cuttings deived rom generative branches. The field tial with factorial treatments was designed in a random¬ ized block with three replicates and 9 plants per plot The varieties tested were : a) Lampung Daun Lebar (LDL), b) Merapin, and c) Kuching. The organic fertilizer used wee : (a) Control, (b) 800 g OST (organic soil treatment) per plant per year and (c) 20 kg cattle manure per plant per year. The esults indicated that Kuching it the best in terms of the number and length of pimary branches, canopy area, number ofberries per spike, and spike length. Fertilizer ■tauuuuts as well as its interaction with varieties had no significant efect on yield at the first harvest. Catle manure had significant effect on to spiked and dried berries it die second harvest However, based on toul production of dried teed*, var. LDL and Kuching gave a significantly higher yield compared with Merapin.
TANGGAP TIGA VARIETAS LADA PERDU TERHADAP PUPUK ORGANIK YANG NURYANI; PASRIL WAHID
Jurnal Penelitian Tanaman Industri Vol 4, No 5 (1999): Januari, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v4n5.1999.135-139

Abstract

Response of three bushy black pepper vaieties to organic fetilizerResponse of three bushy black pepper varieties to organic fertilizer was studied in Sukamulya Research Insulation, rom February 1992 to August 1995. The plant materials used wee pepper cuttings deived rom generative branches. The field tial with factorial treatments was designed in a random¬ ized block with three replicates and 9 plants per plot The varieties tested were : a) Lampung Daun Lebar (LDL), b) Merapin, and c) Kuching. The organic fertilizer used wee : (a) Control, (b) 800 g OST (organic soil treatment) per plant per year and (c) 20 kg cattle manure per plant per year. The esults indicated that Kuching it the best in terms of the number and length of pimary branches, canopy area, number ofberries per spike, and spike length. Fertilizer ■tauuuuts as well as its interaction with varieties had no significant efect on yield at the first harvest. Catle manure had significant effect on to spiked and dried berries it die second harvest However, based on toul production of dried teed*, var. LDL and Kuching gave a significantly higher yield compared with Merapin.
PENETAPAN KERAGAMAN GENETIK NILAM (Pogostemon sp.) HASIL FUSI PROTOPLAS DENGAN TEKNIK RAPD YANG NURYANI; OTIH ROSTIANA; CHEPPY SYUKUR
Jurnal Penelitian Tanaman Industri Vol 8, No 2 (2002): Juni, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n2.2002.39-44

Abstract

Keragaman genetik dan kckerabatan tanaman nilam hasil fusi protoplas antara Nilam Jawa (Girilaya) dan Nilam Aceh (Sidikalang dan TT 75) dianalisis dengan menggunakan penanda RAPD. Bahan tanaman yang digunakan dalam penelitian ini adalah 9 genotipa yang tcrdiri dari 3 tetua dan 6 tanaman hibrida somatik (9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 dan 9 II 10). Primer yang digunakan dalam analisis tcrdiri atas 5 primer acak yaitu OPD 03, OPD 20, OPH 09, OPH 19 dan Abi 117.17. DNA dickstraksi dengan metode OROZCO-CASTJLLO et al. (1994) yang sudah dimodifikasi. Konsentrasi DNA ditetapkan dengan metode sambrook el al. (1989) dengan pcrbandingan kuantifikasi spektrofotometrik. Koefisien kemiripan dan kckerabatan antar genotipa dianalisis dengan menggunakan program NTsys ver. 1.80 dan UPGMA untuk menentukan sidik gerombol dan dendogram. Hasil analisis menunjukkan bahwa koefisien kemiipan dari amplifikasi DNA dengan 5 primer acak dari ke- 9 genotipa berkisar antara 0.48-1.0. Berdasarkan niatrik jarak genetik, kescmbilan genotipa tanaman yang diuji membentuk 2 kelompok besar yaitu kelompok I, tetua Girilaya (Nilam Jawa) dan kelompok II tcrdiri dari tetua Sidikalang dan TT 75 (Nilam Aceh) serta hibrida somatik. Kelompok II, tcrbagi menjadi dua sub kelompok yaitu sub kelompok I (9 II 33 dan 9 II 7) dan sub kelompok II yang tcrdii dari sub-sub kelompok II-I (9 II 21, S, TT 75) dan sub-sub kelompok II-II (2 IV 8, 9 IV 14, 9 II 10).Kata kunci: Pogostemon sp., fusi protoplas, keragaman genetik, RAPD ABSTRACTS Assessment of genetic variability of patchoulli (Pogostemon sp.,) derived from protoplast fussion using RAPD Somatic hybrids of Pogostemon heyneaneus (cv. Girilaya) X P. cablin (cv. Sidikalang and TT 75) were tested for their genetic variability and relationship. The somatic hybrids tested were 9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 and 9 II 10. DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al. (1994) and quantified spectrophotometrically according to SAMBROOK el al. (1989). Five random primers, OPD 03. OPD 20, OPH 09. OPH 19 and Abi 117.17, were applied to amplify the extracted DNA. The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. Index of similarity among genotypes were calculated by using NTsys ver. 1.80 program. Then, cluster analyses to perform dendogram were achieved based on similarity estimates by using the Unweighted Pair-Group Method Arithmetic Average (UPGMA). Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0.48 to 1.0. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli (Sidikalang and TT 75), and the somatic hybrids. The second group was then classified into 2 minor groups. First group consisted of somatic hybrids nos. 9 II 33 and 9 II 7, while the second were classified into 2 groups which consisted nos. 9 II 21, S, TT 75 and nos. 2 IV 8, 9 IV 14 and 9 II 10.Key words : Pogostemon sp., protoplast fusion, genetic variability, RAPD
TANGGAP BEBERAPA KLON NILAM (Pogostemon cablin Benth.) TERHADAP NEMATODA PELUBANG AKAR (Radopholus simitis Cobb.) YANG NURYANI; CHEPPY SYUKUR; RITA HARNI; YELNI TITIS; REPIANYO REPIANYO; IKA MUSTIKA
Jurnal Penelitian Tanaman Industri Vol 5, No 3 (1999): Desember, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n3.1999.103-108

Abstract

Response of some patchouli clones (Pogostemon cablin Benth.) to burrowing nematode (Radopholus similis Cobb.)One of the seious poblems on patchouli is infection of plant parasitic nematode. Nine clones of Aceh patchouli (Pogostemon cablin Benth.) namely KJ (oiginated from tissue cultue) Meulaboh-2, Sidikalang, Lhokseumawe-2, Cisaroni . Cirateun, Tapak Tuan. Aceh Merah. Situak-2, and one clone of Java patchouli (P. heyneanus Benth.) namely Giilaya, were tested to infection of buowing nematode (Radopholus similis Cobb.) in the glasshouse of Research Institute for Spice and Medicinal Crops, Bogor fom July to November 1997. Each clone was inoculated with 500 of R. similis. An expeiment have been conducted to know the esponse of some patchouli cultivars against nematode. The expeiment was arranged in a Completely Randomized Design with thee replicates. The esults showed that the infection of R. similis reduced root weight greater than that of shoot weight, the colour of leaf became reddish or dark purple. Based on the development rate of nematode and level of damage, Giilaya appeared to be resistant, whereas Sidikalang, Cisaroni and Situak-2 clones wee slightly susceptible. KJ. Lhokseumawe-2, Cirateun. Tapak Tuan and Aceh Merah clones were susceptible, whereas Meulaboh-2 were highly susceptible against R. similis. Characteistic of Java clone that esistance and Aceh clone that shyhtly susceptible to R. similis may be useful for providing new vaieties with high content of oil and resistant to nematode.
Co-Authors Cheppy Syukur IKA MUSTIKA OTIH ROSTIANA Pasril Wahid REPIANYO REPIANYO RITA HARNI YELNI TITIS