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PENGELOMPOKAN POHON INDUK CENGKEH TERPILIH DI KABUPATEN SUMEDANG BERDASARKAN KARAKTER MORFOLOGI / Clustering of Progeny Clove Accessions from Cimanggu Population in Sumedang Based on the Morphologycal Characters Cheppy Syukur; nFN Sukarman; Nurliani Bermawie
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.29-36

Abstract

Cimanggu progeny clove has been distributed to almost of centre production, such as in Sumedang. However, their genotypes are still unidentified. Therefore, clustering cloves accession population in Sumedang is necessary. The objectives of this study is to cluster and determine phylogenetic relationship of Cimanggu progeny clove population in Sumedang base on morphological characters. The experiment was conducted in Sumedang (West, Java) from 2013 until 2014, by using 10 selected accessions (healthy, more than 40 years old, productivity is ≥ 20 kg dried flower). Variables were observed include morphological leaf, flower and flower production. To distinguish genotype from each accession was conducted by cluster analyzed, while to identify the variables which were related to the clusters formation, used correspondence analysis. The results indicated, that clove population Sumedang can be classified into three clusters as followed: the first cluster are Syar 43 and 46 accessions, characterized by greenish orange and greenish purple of young leaves tip with flower tube greenish red (GR 180B), the second cluster are Syar 44 and 45 accession, characterized by tip reddish purple young leaves and greenish red (GR181B) flower tubes color, and the third cluster are Syar 47, 49, 50, 51 and 52 accessions, characterized by orange young leaves tips and greenish red (GR180C) flower tubes. This information can be utilized to support releasing Cimanggu clove variety.Keywords: Syzygium aromaticum, clustering, characters, morphology
PENETAPAN KERAGAMAN GENETIK NILAM (Pogostemon sp.) HASIL FUSI PROTOPLAS DENGAN TEKNIK RAPD YANG NURYANI; OTIH ROSTIANA; CHEPPY SYUKUR
Jurnal Penelitian Tanaman Industri Vol 8, No 2 (2002): Juni, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n2.2002.39-44

Abstract

Keragaman genetik dan kckerabatan tanaman nilam hasil fusi protoplas antara Nilam Jawa (Girilaya) dan Nilam Aceh (Sidikalang dan TT 75) dianalisis dengan menggunakan penanda RAPD. Bahan tanaman yang digunakan dalam penelitian ini adalah 9 genotipa yang tcrdiri dari 3 tetua dan 6 tanaman hibrida somatik (9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 dan 9 II 10). Primer yang digunakan dalam analisis tcrdiri atas 5 primer acak yaitu OPD 03, OPD 20, OPH 09, OPH 19 dan Abi 117.17. DNA dickstraksi dengan metode OROZCO-CASTJLLO et al. (1994) yang sudah dimodifikasi. Konsentrasi DNA ditetapkan dengan metode sambrook el al. (1989) dengan pcrbandingan kuantifikasi spektrofotometrik. Koefisien kemiripan dan kckerabatan antar genotipa dianalisis dengan menggunakan program NTsys ver. 1.80 dan UPGMA untuk menentukan sidik gerombol dan dendogram. Hasil analisis menunjukkan bahwa koefisien kemiipan dari amplifikasi DNA dengan 5 primer acak dari ke- 9 genotipa berkisar antara 0.48-1.0. Berdasarkan niatrik jarak genetik, kescmbilan genotipa tanaman yang diuji membentuk 2 kelompok besar yaitu kelompok I, tetua Girilaya (Nilam Jawa) dan kelompok II tcrdiri dari tetua Sidikalang dan TT 75 (Nilam Aceh) serta hibrida somatik. Kelompok II, tcrbagi menjadi dua sub kelompok yaitu sub kelompok I (9 II 33 dan 9 II 7) dan sub kelompok II yang tcrdii dari sub-sub kelompok II-I (9 II 21, S, TT 75) dan sub-sub kelompok II-II (2 IV 8, 9 IV 14, 9 II 10).Kata kunci: Pogostemon sp., fusi protoplas, keragaman genetik, RAPD ABSTRACTS Assessment of genetic variability of patchoulli (Pogostemon sp.,) derived from protoplast fussion using RAPD Somatic hybrids of Pogostemon heyneaneus (cv. Girilaya) X P. cablin (cv. Sidikalang and TT 75) were tested for their genetic variability and relationship. The somatic hybrids tested were 9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 and 9 II 10. DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al. (1994) and quantified spectrophotometrically according to SAMBROOK el al. (1989). Five random primers, OPD 03. OPD 20, OPH 09. OPH 19 and Abi 117.17, were applied to amplify the extracted DNA. The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. Index of similarity among genotypes were calculated by using NTsys ver. 1.80 program. Then, cluster analyses to perform dendogram were achieved based on similarity estimates by using the Unweighted Pair-Group Method Arithmetic Average (UPGMA). Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0.48 to 1.0. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli (Sidikalang and TT 75), and the somatic hybrids. The second group was then classified into 2 minor groups. First group consisted of somatic hybrids nos. 9 II 33 and 9 II 7, while the second were classified into 2 groups which consisted nos. 9 II 21, S, TT 75 and nos. 2 IV 8, 9 IV 14 and 9 II 10.Key words : Pogostemon sp., protoplast fusion, genetic variability, RAPD
TANGGAP BEBERAPA KLON NILAM (Pogostemon cablin Benth.) TERHADAP NEMATODA PELUBANG AKAR (Radopholus simitis Cobb.) YANG NURYANI; CHEPPY SYUKUR; RITA HARNI; YELNI TITIS; REPIANYO REPIANYO; IKA MUSTIKA
Jurnal Penelitian Tanaman Industri Vol 5, No 3 (1999): Desember, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n3.1999.103-108

Abstract

Response of some patchouli clones (Pogostemon cablin Benth.) to burrowing nematode (Radopholus similis Cobb.)One of the seious poblems on patchouli is infection of plant parasitic nematode. Nine clones of Aceh patchouli (Pogostemon cablin Benth.) namely KJ (oiginated from tissue cultue) Meulaboh-2, Sidikalang, Lhokseumawe-2, Cisaroni . Cirateun, Tapak Tuan. Aceh Merah. Situak-2, and one clone of Java patchouli (P. heyneanus Benth.) namely Giilaya, were tested to infection of buowing nematode (Radopholus similis Cobb.) in the glasshouse of Research Institute for Spice and Medicinal Crops, Bogor fom July to November 1997. Each clone was inoculated with 500 of R. similis. An expeiment have been conducted to know the esponse of some patchouli cultivars against nematode. The expeiment was arranged in a Completely Randomized Design with thee replicates. The esults showed that the infection of R. similis reduced root weight greater than that of shoot weight, the colour of leaf became reddish or dark purple. Based on the development rate of nematode and level of damage, Giilaya appeared to be resistant, whereas Sidikalang, Cisaroni and Situak-2 clones wee slightly susceptible. KJ. Lhokseumawe-2, Cirateun. Tapak Tuan and Aceh Merah clones were susceptible, whereas Meulaboh-2 were highly susceptible against R. similis. Characteistic of Java clone that esistance and Aceh clone that shyhtly susceptible to R. similis may be useful for providing new vaieties with high content of oil and resistant to nematode.
EFEKTIVITAS EKSTRAK ETANOL BIJI MIMBA, MAHONI DAN SUREN TERHADAP LARVA Tenebrio molitor Linnaeus (Tenebrionidae: Coleoptera) Wida Darwiati; Ujang Wawan Darmawan; Cheppy Syukur
Buletin Penelitian Tanaman Rempah dan Obat Vol 31, No 1 (2020): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v31n1.2020.40-47

Abstract

Neem, mahogany, and surian seeds contain secondary metabolites that can be used as biopesticides. This study aimed to determine the effectiveness of neem, mahogany, and surian seed extracts to the larvae of the Tenebrio molitor L. (Tenebrionidae: Coleoptera) at laboratory scale. The stages of the research were the preparation of seed extracts, phytochemical analysis of the extracts, and efficacy of extracts against T. molitor larvae. Dried seeds of neem, surian, and mahogany (water content < 10%) were ground (100 mesh) and macerated with 95% ethanol solvent for seven hours at 70 oC. The alkaloids, flavonoids, tannins, saponins, and steroids/the triterpenoid were then analyzed. The effectiveness of the extracts was  testedagainst the 3rd larva of T. molinor. The experiment designed in Randomize block design, three replications, each with ten larvae. The first factor was seed extracts (neem, surian, and mahogany), while the second factor was five levels of extract concentration (0 mg;l-1, 9 mg.l-1, 15 mg.l-1, 30 mg.l-1 and 45 mg.l-1). Phytochemical analysis showed that mahogany seeds contained alkaloids and triterpenoids. Meanwhile, surian seeds contained tannin, alkaloid, steroid/triterpenoid, but neem seeds contained saponin and alkaloids. The mahogany, surian, and neem seed  extracts were effective in causing significant mortality to T. molitor larvae. Ethanol extract of neem seeds was more toxic to larvae (LC50 = 0.72 mg.l-1), compared to mahogany (LC50 = 5.2 mg.l-1) and surian (LC50 = 7.02 mg.l-1). Mimba seed extract is more prospective to be developed as a botanical insecticide.
ADAPTASI DELAPAN NOMOR HARAPAN KUNYIT (Curcuma domestica Vahl.) TOLERAN NAUNGAN Sitti Fatimah Syahid; Cheppy Syukur; Nathalini Nova Kristina; Joko Pitono
Buletin Penelitian Tanaman Rempah dan Obat Vol 23, No 2 (2012): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v23n2.2012.%p

Abstract

Turmeric cultivation by farmer is mainly conducted under shading, so it needed turmeric superior seeds especially for shading cultivation. The aim of the research was to analiysis the adaptability based on yield and quality stability of turmeric promising lines under shading on three different agroecology Adaptation test for releasing the superior variety of crops was conducted at three locations of turmeric central production in Bringin 464 m asl, Nogosari, 425 m asl, and Simo 484 m asl. Tested was done in 2008 until 2010 under famer’s teak plantations. Eight promising lines of turmeric out of 70 accessions selected under artificial shading season in year of 2007/2008 at Cicurug, Sukabumi (West Java) and one local number were used as materials to be tested. The experiment was arranged in Randomized Block Design with three replications. Each block consisted of 48 plants. The observed parameters were growth component, yield, and curcumin content. Combined varian analysis result showed that there was no interaction effect between genotype and environment on the yield and curcumin content. However, all of the tested genotypes were signicifantly differed for their curcumin content. Turmeric promising line Cudo 04, with rhizome yield 0f 7.4 ton ha-1, had the highest curcumin (7.05%) and essential oil content (4.77%), and tolerant to the leaf spot disease. Therefore, this line is recommended to be released as a superior variety tolerant to shading. Based on its curcumin content, this line was also found as a superior one to be developed by herbal medicine industry.
KERAGAAN KARAKTER MORFOLOGI, HASIL DAN MUTU ENAM AKSESI JAHE PUTIH KECIL DI TIGA AGROEKOLOGI BERBEDA Cheppy Syukur; Muhammad Yusron; Octivia Trisilawati
Buletin Penelitian Tanaman Rempah dan Obat Vol 26, No 1 (2015): Buletin Penelitian Tanaman Rempah dan Obat
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v26n1.2015.1-10

Abstract

Jahe merupakan tanaman yang memerlukan lahan subur dan pupuk dosis tinggi untuk menghasilkan rimpang yang optimal. Ketersediaan lahan subur semakin terbatas, sedangkan harga pupuk semakin tinggi, sehingga diperlukan varietas jahe yang toleran ditanam di lahan marginal dengan input pupuk rendah. Penelitian ini bertujuan untuk mendapatkan keragaan karakter morfologi, hasil dan mutu aksesi jahe putih kecil pada budidaya dengan input pupuk rendah, atau tumbuh baik pada kondisi lahan marginal. Evaluasi plasma nutfah jahe toleran lahan marginal di lakukan di Kabupaten Lebak (Banten), Kulonprogo (Yogyakarta) dan Bantul (Yogyakarta). Bahan tanaman yang digunakan enam aksesi jahe putih kecil (JPK), menggunakan rancangan acak kelompok diulang empat kali. Pengamatan dilakukan terhadap karakter pertumbuhan tanaman (populasi tanaman, tinggi tanaman, jumlah anakan, jumlah daun, panjang daun, lebar daun, diameter batang) dan produksi rimpang segar serta kadar gingerol. Data dianalisis menggunakan ANOVA dilanjutkan dengan DMRT 0,5% apabila berbeda nyata. Hasil evaluasi keenam aksesi yang ditanam di lahan marginal dengan pemupukan dosis rendah, menunjukkan respon yang berbeda pada fase pertumbuhan maupun produksi. Aksesi 02 menunjukkan karakter diameter batang yang stabil di tiga lokasi, sedangkan aksesi lainnya tidak stabil. Aksesi 04 menunjukkan stabilitas pada karakter produksi rimpang segar dan kadar gingerol di tiga lokasi. Aksesi 04 menghasilkan bobot rimpang segar tertinggi (546,92 g rumpun-1) dan kadar gingerol lebih dari 1%, sehingga aksesi 04 berpotensi untuk dikembangkan di lahan marginal dengan input pupuk rendah.
PENGELOMPOKAN POHON INDUK CENGKEH TERPILIH DI KABUPATEN SUMEDANG BERDASARKAN KARAKTER MORFOLOGI / Clustering of Progeny Clove Accessions from Cimanggu Population in Sumedang Based on the Morphologycal Characters Cheppy Syukur; nFN Sukarman; Nurliani Bermawie
Jurnal Penelitian Tanaman Industri Vol 22, No 1 (2016): Maret, 2016
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v22n1.2016.29-36

Abstract

Cimanggu progeny clove has been distributed to almost of centre production, such as in Sumedang. However, their genotypes are still unidentified. Therefore, clustering cloves accession population in Sumedang is necessary. The objectives of this study is to cluster and determine phylogenetic relationship of Cimanggu progeny clove population in Sumedang base on morphological characters. The experiment was conducted in Sumedang (West, Java) from 2013 until 2014, by using 10 selected accessions (healthy, more than 40 years old, productivity is ≥ 20 kg dried flower). Variables were observed include morphological leaf, flower and flower production. To distinguish genotype from each accession was conducted by cluster analyzed, while to identify the variables which were related to the clusters formation, used correspondence analysis. The results indicated, that clove population Sumedang can be classified into three clusters as followed: the first cluster are Syar 43 and 46 accessions, characterized by greenish orange and greenish purple of young leaves tip with flower tube greenish red (GR 180B), the second cluster are Syar 44 and 45 accession, characterized by tip reddish purple young leaves and greenish red (GR181B) flower tubes color, and the third cluster are Syar 47, 49, 50, 51 and 52 accessions, characterized by orange young leaves tips and greenish red (GR180C) flower tubes. This information can be utilized to support releasing Cimanggu clove variety.Keywords: Syzygium aromaticum, clustering, characters, morphology
PENETAPAN KERAGAMAN GENETIK NILAM (Pogostemon sp.) HASIL FUSI PROTOPLAS DENGAN TEKNIK RAPD YANG NURYANI; OTIH ROSTIANA; CHEPPY SYUKUR
Jurnal Penelitian Tanaman Industri Vol 8, No 2 (2002): Juni, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n2.2002.39-44

Abstract

Keragaman genetik dan kckerabatan tanaman nilam hasil fusi protoplas antara Nilam Jawa (Girilaya) dan Nilam Aceh (Sidikalang dan TT 75) dianalisis dengan menggunakan penanda RAPD. Bahan tanaman yang digunakan dalam penelitian ini adalah 9 genotipa yang tcrdiri dari 3 tetua dan 6 tanaman hibrida somatik (9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 dan 9 II 10). Primer yang digunakan dalam analisis tcrdiri atas 5 primer acak yaitu OPD 03, OPD 20, OPH 09, OPH 19 dan Abi 117.17. DNA dickstraksi dengan metode OROZCO-CASTJLLO et al. (1994) yang sudah dimodifikasi. Konsentrasi DNA ditetapkan dengan metode sambrook el al. (1989) dengan pcrbandingan kuantifikasi spektrofotometrik. Koefisien kemiripan dan kckerabatan antar genotipa dianalisis dengan menggunakan program NTsys ver. 1.80 dan UPGMA untuk menentukan sidik gerombol dan dendogram. Hasil analisis menunjukkan bahwa koefisien kemiipan dari amplifikasi DNA dengan 5 primer acak dari ke- 9 genotipa berkisar antara 0.48-1.0. Berdasarkan niatrik jarak genetik, kescmbilan genotipa tanaman yang diuji membentuk 2 kelompok besar yaitu kelompok I, tetua Girilaya (Nilam Jawa) dan kelompok II tcrdiri dari tetua Sidikalang dan TT 75 (Nilam Aceh) serta hibrida somatik. Kelompok II, tcrbagi menjadi dua sub kelompok yaitu sub kelompok I (9 II 33 dan 9 II 7) dan sub kelompok II yang tcrdii dari sub-sub kelompok II-I (9 II 21, S, TT 75) dan sub-sub kelompok II-II (2 IV 8, 9 IV 14, 9 II 10).Kata kunci: Pogostemon sp., fusi protoplas, keragaman genetik, RAPD ABSTRACTS Assessment of genetic variability of patchoulli (Pogostemon sp.,) derived from protoplast fussion using RAPD Somatic hybrids of Pogostemon heyneaneus (cv. Girilaya) X P. cablin (cv. Sidikalang and TT 75) were tested for their genetic variability and relationship. The somatic hybrids tested were 9 II 33, 9 II 21, 2 IV 8, 9 IV14, 9 II 7 and 9 II 10. DNA of the plant materials used were extracted by using the modified method of orozcocastulo et al. (1994) and quantified spectrophotometrically according to SAMBROOK el al. (1989). Five random primers, OPD 03. OPD 20, OPH 09. OPH 19 and Abi 117.17, were applied to amplify the extracted DNA. The genetic relationship among the somatic hybrids were estimated by using the index of similarity to perform genctical matrix and dendogram. Index of similarity among genotypes were calculated by using NTsys ver. 1.80 program. Then, cluster analyses to perform dendogram were achieved based on similarity estimates by using the Unweighted Pair-Group Method Arithmetic Average (UPGMA). Results showed that index of similarities of the amplified DNA from 5 random primers ranged from 0.48 to 1.0. The somatic hybrids and their parental plants subjected to RAPD analyses were classified into 2 major groups, first, the parental group of Java patchouli and second, others parental plants, Aceh patchouli (Sidikalang and TT 75), and the somatic hybrids. The second group was then classified into 2 minor groups. First group consisted of somatic hybrids nos. 9 II 33 and 9 II 7, while the second were classified into 2 groups which consisted nos. 9 II 21, S, TT 75 and nos. 2 IV 8, 9 IV 14 and 9 II 10.Key words : Pogostemon sp., protoplast fusion, genetic variability, RAPD
TANGGAP BEBERAPA KLON NILAM (Pogostemon cablin Benth.) TERHADAP NEMATODA PELUBANG AKAR (Radopholus simitis Cobb.) YANG NURYANI; CHEPPY SYUKUR; RITA HARNI; YELNI TITIS; REPIANYO REPIANYO; IKA MUSTIKA
Jurnal Penelitian Tanaman Industri Vol 5, No 3 (1999): Desember, 1999
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n3.1999.103-108

Abstract

Response of some patchouli clones (Pogostemon cablin Benth.) to burrowing nematode (Radopholus similis Cobb.)One of the seious poblems on patchouli is infection of plant parasitic nematode. Nine clones of Aceh patchouli (Pogostemon cablin Benth.) namely KJ (oiginated from tissue cultue) Meulaboh-2, Sidikalang, Lhokseumawe-2, Cisaroni . Cirateun, Tapak Tuan. Aceh Merah. Situak-2, and one clone of Java patchouli (P. heyneanus Benth.) namely Giilaya, were tested to infection of buowing nematode (Radopholus similis Cobb.) in the glasshouse of Research Institute for Spice and Medicinal Crops, Bogor fom July to November 1997. Each clone was inoculated with 500 of R. similis. An expeiment have been conducted to know the esponse of some patchouli cultivars against nematode. The expeiment was arranged in a Completely Randomized Design with thee replicates. The esults showed that the infection of R. similis reduced root weight greater than that of shoot weight, the colour of leaf became reddish or dark purple. Based on the development rate of nematode and level of damage, Giilaya appeared to be resistant, whereas Sidikalang, Cisaroni and Situak-2 clones wee slightly susceptible. KJ. Lhokseumawe-2, Cirateun. Tapak Tuan and Aceh Merah clones were susceptible, whereas Meulaboh-2 were highly susceptible against R. similis. Characteistic of Java clone that esistance and Aceh clone that shyhtly susceptible to R. similis may be useful for providing new vaieties with high content of oil and resistant to nematode.
ADAPTASI DELAPAN NOMOR HARAPAN KUNYIT (Curcuma domestica Vahl.) TOLERAN NAUNGAN Sitti Fatimah Syahid; Cheppy Syukur; Nathalini Nova Kristina; Joko Pitono
Buletin Penelitian Tanaman Rempah dan Obat Vol 23, No 2 (2012): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v23n2.2012.%p

Abstract

Turmeric cultivation by farmer is mainly conducted under shading, so it needed turmeric superior seeds especially for shading cultivation. The aim of the research was to analiysis the adaptability based on yield and quality stability of turmeric promising lines under shading on three different agroecology Adaptation test for releasing the superior variety of crops was conducted at three locations of turmeric central production in Bringin 464 m asl, Nogosari, 425 m asl, and Simo 484 m asl. Tested was done in 2008 until 2010 under famer’s teak plantations. Eight promising lines of turmeric out of 70 accessions selected under artificial shading season in year of 2007/2008 at Cicurug, Sukabumi (West Java) and one local number were used as materials to be tested. The experiment was arranged in Randomized Block Design with three replications. Each block consisted of 48 plants. The observed parameters were growth component, yield, and curcumin content. Combined varian analysis result showed that there was no interaction effect between genotype and environment on the yield and curcumin content. However, all of the tested genotypes were signicifantly differed for their curcumin content. Turmeric promising line Cudo 04, with rhizome yield 0f 7.4 ton ha-1, had the highest curcumin (7.05%) and essential oil content (4.77%), and tolerant to the leaf spot disease. Therefore, this line is recommended to be released as a superior variety tolerant to shading. Based on its curcumin content, this line was also found as a superior one to be developed by herbal medicine industry.