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Pestalotiopsis desseminata PADA JAMBU MENTE BIOLOGI DAN INTERAKSINYA DENGAN Helopeltis antonii ESTHER M. ADHI; SUPRIADI SUPRIADI; S. RAHAYUNINGSIH; D. KILIN; NURI KARYANI
Jurnal Penelitian Tanaman Industri Vol 6, No 3 (2000): Desember, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n3.2000.66-72

Pestalotiopsis desseminata on cashew: its biology and interaction with Helopeltis antoniiPestalotiopsis desseminata is one of pathogens causing leaf spots. die-back of shoots and inflorescence of cashew plant. It is assumed there is an interaction between the attack of Helopeltis antonii and the fungus in Ihe ield so that the damage becomes more devastating. The objectives of (his research were to investigate several aspects of fungal biology (pathogenicity, mycelial growth, acervuli production, sensitivity to fungicides) and its interaction with //. antonii. The esearch was carried out from April 1999 to March 2000 at the laboratory and green house of Pest and Disease Department Research Institute for Spice and Medicinal Crops, Bogor. /' desseminata was diectly isolated from cashew leaves. Pathogenicity test of P. desseminata isolate was conducted on cashew seedlings. The fungal growth and fungicide effects were carried out in vitro on agar medium. The interaction between P. desseminata and H. antonii was examined by inoculating the fungus and insect, either individually or in combination, on cashew seedlings. The results showed that P. desseminata isolate fomis black spherical acervuli containing oblongs conidia with 5 cells, and the outermost cell has 3 flagellate. Acervuli could only be produced on the cultue illuminated continuously by 600 lux translucent lamp. Pathogenicity test of several isolates of /' desseminata on cashew seedlings caused spherical leaf spot symptom, individually or coalesced as bigger leaf spots. In vitro fungal mycelia growth could be suppressed by several fungicides, including bcnomyl 50% (I ppm) and Uiiopanate-mcthyl 70% (10 ppm) Combining inoculation between P. desseminata and //. antonii resulted in moe severe (devastating) die-back compared with individually inoculated.

SERANGAN Mimegralla coeruleifrons Macquart PADA TANAMAN JAHE DAN PERANANNYA DALAM MEMBAWA PATOGEN PENYAKIT LAYU RODIAH BALFAS; SUPRIADI SUPRIADI; NURI KARYANI; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.123-127

The attack of Mimegralla coeruleifrons on ginger plants and its role in carrying pathogens of wilt diseaseRhizome flics Mimegralla coeruleifrons (Diplera; Micropezidae) ac frequently oten found in the diseased ginger rhizome caused by Ralstonia solanacearum, bill it has nol been known which one made the irst damage. Experiments were conducted at laboratory and green house of the Research Institute for Spice and Medicinal Cops from September 1998 to I'cbruary 2000. to examine the attack of M coerulefrons on healthy and disease ginger plants and its role in carrying the pauiogen. Hie experiments consisted of three parts, ink-station of the fly in healthy and diseased ginger plants, disease tansmission and detection of R solanacearum in M coeruleifrons larvae and adults, fiingcr plants were grown in pot containing, sterile media The pots were placed in the field and the plants were inoculated with R solanacearum and iininoculatcd. then they were caged or uncaged. Adult lies (33 - 35 pairs) were introduced into the cage. The uncaged plants were allowed to be naturally invested by the flies. Disease transmission used adult Hies which were collected from diseased ginger plantation. The lies were fed with suspension of R solanacearum. then introduced into cages containing healthy ginger plants. The flies were also directly caged on healthy plants Detection of/?, solanacearum in larvae and adults was carried out by isolation on selective medium and ELISA techniques. Results showed that M coerulefrons mostly attacked the inoculated ginger plants. Tliere was no evidence of Iransmission of R solanacearum by the fly. whereas nearly 50% of the ield collected lics carried R solanacearum. These results tend to suggest that the attack of M. coerulerons occured ater the ginger has been infected by wilt disease and the ly can be a carrier of/? solanacearum.

Keefektifan Beberapa Formula Fungisida Nabati Eugenol dan Sitronella terhadap Phytophthora palmivora Bult. Asal Kakao Rita Harni; Widi Amaria; Supriadi Supriadi
Jurnal Tanaman Industri dan Penyegar Vol 4, No 1 (2013): Buletin Riset Tanaman Rempah dan Aneka Tanaman Industri
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jtidp.v4n1.2013.p11-18

Phytophthora palmivora merupakan patogen utama pada tanaman kakao di seluruh dunia. Akibat serangan patogen ini menyebabkan kerugian sebesar 25-50%. Pengendalian patogen ini masih mengandalkan fungisida sintetik yang dikhawatirkan berbahaya bagi manusia dan lingkungan. Fungisida nabati yang mengandung minyak atsiri, merupakan alternatif pengendalian yang lebih ramah lingkungan. Penelitian bertujuan mengevaluasi pengaruh formula eugenol, sitronella, asam salisilat dan silikon terhadap pertumbuhan P. palmivora. Penelitian dilakukan di Laboratorium dan Rumah Kaca Balai Penelitian Tanaman Industri dan Penyegar dari Maret sampai Agustus 2012. Minyak cengkeh (CK), serai wangi (SW), asam salisilat (AS) dan silikon cair (SI) digunakan sebagai bahan utama pembuatan formula. Formula yang diuji, yaitu (1) Eugenol, (2) Sitronella, (3) CK+SW, (4) SW+SI, (5) SW+AS, (6) CK+SI, dan (7) CK+AS. Percobaan dirancang secara acak lengkap dengan 5 ulangan. Pengujian formula terhadap pertumbuhan P. palmivora in vitro dilakukan dengan menginokulasikan potongan agar berisi kultur jamur pada permukaan PDA yang mengandung formula, sedangkan pengujian formula pada buah dan bibit kakao diuji di rumah kaca. Kadar fenol dan lignin dalam jaringan daun bibit kakao dianalisis untuk mengetahui mekanisme pertahanan tanaman terhadap infeksi patogen. Hasil penelitian menunjukkan bahwa semua formula yang diuji menghambat pertumbuhan dan biomassa P. palmivora pada kondisi in vitro, dan perkembangan gejala penyakit pada buah serta bibit kakao. Formula eugenol + asam salisilat mampu menghambat total pertumbuhan dan bioassay patogen pada media PDA, menekan perkembangan penyakit 65,2% pada buah dan 66,25% pada bibit, serta meningkatkan kadar senyawa fenol dan lignin dalam jaringan daun.Kata Kunci: Kakao, Phytophthora palmivora, eugenol, sitronella, fungisida nabatiPhytophthora palmivora is a major pathogen of cacao plants on the world. This pathogen caused 25-50% losses. The pathogen is commonly controlled with synthetic fungicide uses which may hazard for human and environment if used unwisely. Botanical fungicides containing essential oils offer more environmentally friendly control method. The study aimed to evaluate the effects of seven botanical fungicide formulas containing clove oil and citronella oil on P. palmivora. The study was conducted in the Laboratory and Greenhouse of the Indonesian Research Institute for Industrial and Beverage Crops from March to August 2012. A randomized complete designed with five replicates was used. Seven formulas tested were: (1) eugenol (CK), (2) citronella (SW), (3) CK+SW, (4) SW+ silicone (SI), (5) SW+salycilic acid (AS), (6) CK+SI, dan (7) CK+AS. The effects of formulas on growth and biomass of P. palmivora in vitro were tested by culturing the pathogen on solid PDA medium whereas its effect on disease developments was tested by inoculating cacao pods on fruits and seedlings. In addition, the phenol and lignin contents in the inoculated seedlings were assessed to support disease inhibition mechanism. The results showed that all the formulas tested inhibited the growth and biomass of P. palmivora in vitro. The highest inhibition was shown by the formula of clove oil mixed with salicylic acid. Disease developments on inoculated cocoa pods and seedlings reduced of 66.25% and 65.2% respectively, and increased levels of lignin and phenol in the leaves.

POTENSI BAKTERI ANTAGONIS DALAM MENEKAN PERKEMBANGAN PENYAKIT LAYU BAKTERI JAHE KARDEN MULYA; SUPRIADI SUPRIADI; ESTHER M. ADHI; SRI RAHAYU; NURI KARYANI
Jurnal Penelitian Tanaman Industri Vol 6, No 2 (2000): September, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n2.2000.37-43

Potency of antagonist bacteria in inhibiting the bacterial wilt disease progress gingerBacterial wilt disease caused by Pseudomonas solanacearum is an important disease in ginger plant (Zingiber oicinale Rose.) in Indonesia. The objective of this research was to study the effectiveness of biological agents in inhibiting the progess of bacterial wilt on ginger. The research was conducted at the geenhouse of Research Institute for Spice and Medicinal Crops in 1997-1998 then was followed with a ield experiment at Sukamulya Experimental Garden in 1998/1999. At the greenhouse experiment seven kinds of antagonists bacteria were formulated either individually or combination. Pseudomonas fluorescens (PF), P. cepacia (PC) and Bacillus sp. (BC), mixtue of PF + PC, mixtue of PC + BC, mixture of PF + BC, and mixtue of PF + PC + BC were tested in the geenhouse on ginger plant cultivars putih besar grown in the pot containing soil formerly used for growing P. solanacearum infected tomato. I he results of this experiment indicated that the combination of PF+PC+BC significantly suppressed the progress of bacterial wilt disease compaed to control and other tested combination. In the ield experiment carried out at the bacterial wilt disease endemic area the combinations of PF+PC+BC, Trichoderma harzianum (Bit-1) and combination of PF+PC+BC+BII-I were tested with two levels of application, i.e. two applications with two months interval and 4 application with one month interval. The results showed that the application of antagonists bacteria inhibited Ihe bacterial wilt disease progress and signiicantly increased ginger rhizome yield. The yield of the rhizome from the plants teated with different intervals were not different. However, the application of the antagonist bacteria were not able to eradicate ginger infection by Pseudomonas solanacearum thoroughly.

PENYEBAB DAN SERANGGA VEKTOR PENYAKIT KERITING PADA TANAMAN LADA RODIAH BALFAS; SUPRIADI SUPRIADI; T. L. MARDININGSIH; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 8, No 1 (2002): Maret, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n1.2002.7-11

The cause of stunting disease of black pepper (Piper nigrum) in Indonesia has not yet been confirmed cither due o a virus or mycoplaam. However, similar disease found on black pepper plants in the Southeast Asia is caused by Piper yellow mottle virus (PYMV) which is transmitted by Planococcus citri. This expeiments was aimed o examine the cause of the stunting disease and its insect vectors. The expeiment were conducted from October 1998 to May 2001. Diseased vegetative materials of black pepper plants showing stunting disease were collected from Sukamulya, Sukabumi. The potential insect vectors were collected from black pepper plants in Bogor, IP Sukamulya (Sukabumi), Lampung and Bangka. The insects were fed on the diseased plants obtained from Lampung, Bogor and IP Sukamulya, then transferred o the healthy plants. The healthy plants were produced rom true seeds and cutings oiginated rom Bogor and IP Sukamulya. The tested plants were incubated at the green house and examined for disease development. Diseased leaf samples collected rom black pepper plant rom IP. Sukamulya and the transmitted plants were sent to the University of Minnesota, USA for Ihe virus (PYMV) by using ISEM (immunosorbent electron microscope). The result snowed that the leaves samples rom IP. Sukamulya were infected by PYMV. The morphology and size of the virus were similar to those caused stunting disease in the South East Asia. Potential insects vectors found on the diseased black pepper plants were two mealybugs, P. minor and Ferrisia virgata (Hemiptera; Coccoidca: PaaidbcoccidaeX aa well as an aphid Toxoptera aurantii (Hemiptera: Aphidoidea: Aphididae). The first tial on disease transmission by using P. minor, previously reared on the potato tubers, showed one out of ten tested plants produced disease sympom. The subsequent trial using P minor, bred on healthy black pepper seedlings, showed thee out often tested plants developed disease symptoms. None of the aphid transmitted plants developed Ihe disease. This study confirmed thai PYMV b the cause of stunting disease on black pepper in IP Sukamulya and Lampung and P. minor as the insect vecor of the disease.

SERANGAN Mimegralla coeruleifrons Macquart PADA TANAMAN JAHE DAN PERANANNYA DALAM MEMBAWA PATOGEN PENYAKIT LAYU RODIAH BALFAS; SUPRIADI SUPRIADI; NURI KARYANI; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.123-127

The attack of Mimegralla coeruleifrons on ginger plants and its role in carrying pathogens of wilt diseaseRhizome flics Mimegralla coeruleifrons (Diplera; Micropezidae) ac frequently oten found in the diseased ginger rhizome caused by Ralstonia solanacearum, bill it has nol been known which one made the irst damage. Experiments were conducted at laboratory and green house of the Research Institute for Spice and Medicinal Cops from September 1998 to I'cbruary 2000. to examine the attack of M coerulefrons on healthy and disease ginger plants and its role in carrying the pauiogen. Hie experiments consisted of three parts, ink-station of the fly in healthy and diseased ginger plants, disease tansmission and detection of R solanacearum in M coeruleifrons larvae and adults, fiingcr plants were grown in pot containing, sterile media The pots were placed in the field and the plants were inoculated with R solanacearum and iininoculatcd. then they were caged or uncaged. Adult lies (33 - 35 pairs) were introduced into the cage. The uncaged plants were allowed to be naturally invested by the flies. Disease transmission used adult Hies which were collected from diseased ginger plantation. The lies were fed with suspension of R solanacearum. then introduced into cages containing healthy ginger plants. The flies were also directly caged on healthy plants Detection of/?, solanacearum in larvae and adults was carried out by isolation on selective medium and ELISA techniques. Results showed that M coerulefrons mostly attacked the inoculated ginger plants. Tliere was no evidence of Iransmission of R solanacearum by the fly. whereas nearly 50% of the ield collected lics carried R solanacearum. These results tend to suggest that the attack of M. coerulerons occured ater the ginger has been infected by wilt disease and the ly can be a carrier of/? solanacearum.

Pestalotiopsis desseminata PADA JAMBU MENTE BIOLOGI DAN INTERAKSINYA DENGAN Helopeltis antonii ESTHER M. ADHI; SUPRIADI SUPRIADI; S. RAHAYUNINGSIH; D. KILIN; NURI KARYANI
Jurnal Penelitian Tanaman Industri Vol 6, No 3 (2000): Desember, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n3.2000.66-72

Pestalotiopsis desseminata on cashew: its biology and interaction with Helopeltis antoniiPestalotiopsis desseminata is one of pathogens causing leaf spots. die-back of shoots and inflorescence of cashew plant. It is assumed there is an interaction between the attack of Helopeltis antonii and the fungus in Ihe ield so that the damage becomes more devastating. The objectives of (his research were to investigate several aspects of fungal biology (pathogenicity, mycelial growth, acervuli production, sensitivity to fungicides) and its interaction with //. antonii. The esearch was carried out from April 1999 to March 2000 at the laboratory and green house of Pest and Disease Department Research Institute for Spice and Medicinal Crops, Bogor. /' desseminata was diectly isolated from cashew leaves. Pathogenicity test of P. desseminata isolate was conducted on cashew seedlings. The fungal growth and fungicide effects were carried out in vitro on agar medium. The interaction between P. desseminata and H. antonii was examined by inoculating the fungus and insect, either individually or in combination, on cashew seedlings. The results showed that P. desseminata isolate fomis black spherical acervuli containing oblongs conidia with 5 cells, and the outermost cell has 3 flagellate. Acervuli could only be produced on the cultue illuminated continuously by 600 lux translucent lamp. Pathogenicity test of several isolates of /' desseminata on cashew seedlings caused spherical leaf spot symptom, individually or coalesced as bigger leaf spots. In vitro fungal mycelia growth could be suppressed by several fungicides, including bcnomyl 50% (I ppm) and Uiiopanate-mcthyl 70% (10 ppm) Combining inoculation between P. desseminata and //. antonii resulted in moe severe (devastating) die-back compared with individually inoculated.

POTENSI BAKTERI ANTAGONIS DALAM MENEKAN PERKEMBANGAN PENYAKIT LAYU BAKTERI JAHE KARDEN MULYA; SUPRIADI SUPRIADI; ESTHER M. ADHI; SRI RAHAYU; NURI KARYANI
Jurnal Penelitian Tanaman Industri Vol 6, No 2 (2000): September, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n2.2000.37-43

Potency of antagonist bacteria in inhibiting the bacterial wilt disease progress gingerBacterial wilt disease caused by Pseudomonas solanacearum is an important disease in ginger plant (Zingiber oicinale Rose.) in Indonesia. The objective of this research was to study the effectiveness of biological agents in inhibiting the progess of bacterial wilt on ginger. The research was conducted at the geenhouse of Research Institute for Spice and Medicinal Crops in 1997-1998 then was followed with a ield experiment at Sukamulya Experimental Garden in 1998/1999. At the greenhouse experiment seven kinds of antagonists bacteria were formulated either individually or combination. Pseudomonas fluorescens (PF), P. cepacia (PC) and Bacillus sp. (BC), mixtue of PF + PC, mixtue of PC + BC, mixture of PF + BC, and mixtue of PF + PC + BC were tested in the geenhouse on ginger plant cultivars putih besar grown in the pot containing soil formerly used for growing P. solanacearum infected tomato. I he results of this experiment indicated that the combination of PF+PC+BC significantly suppressed the progress of bacterial wilt disease compaed to control and other tested combination. In the ield experiment carried out at the bacterial wilt disease endemic area the combinations of PF+PC+BC, Trichoderma harzianum (Bit-1) and combination of PF+PC+BC+BII-I were tested with two levels of application, i.e. two applications with two months interval and 4 application with one month interval. The results showed that the application of antagonists bacteria inhibited Ihe bacterial wilt disease progress and signiicantly increased ginger rhizome yield. The yield of the rhizome from the plants teated with different intervals were not different. However, the application of the antagonist bacteria were not able to eradicate ginger infection by Pseudomonas solanacearum thoroughly.

PENYEBAB DAN SERANGGA VEKTOR PENYAKIT KERITING PADA TANAMAN LADA RODIAH BALFAS; SUPRIADI SUPRIADI; T. L. MARDININGSIH; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 8, No 1 (2002): Maret, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n1.2002.7-11

The cause of stunting disease of black pepper (Piper nigrum) in Indonesia has not yet been confirmed cither due o a virus or mycoplaam. However, similar disease found on black pepper plants in the Southeast Asia is caused by Piper yellow mottle virus (PYMV) which is transmitted by Planococcus citri. This expeiments was aimed o examine the cause of the stunting disease and its insect vectors. The expeiment were conducted from October 1998 to May 2001. Diseased vegetative materials of black pepper plants showing stunting disease were collected from Sukamulya, Sukabumi. The potential insect vectors were collected from black pepper plants in Bogor, IP Sukamulya (Sukabumi), Lampung and Bangka. The insects were fed on the diseased plants obtained from Lampung, Bogor and IP Sukamulya, then transferred o the healthy plants. The healthy plants were produced rom true seeds and cutings oiginated rom Bogor and IP Sukamulya. The tested plants were incubated at the green house and examined for disease development. Diseased leaf samples collected rom black pepper plant rom IP. Sukamulya and the transmitted plants were sent to the University of Minnesota, USA for Ihe virus (PYMV) by using ISEM (immunosorbent electron microscope). The result snowed that the leaves samples rom IP. Sukamulya were infected by PYMV. The morphology and size of the virus were similar to those caused stunting disease in the South East Asia. Potential insects vectors found on the diseased black pepper plants were two mealybugs, P. minor and Ferrisia virgata (Hemiptera; Coccoidca: PaaidbcoccidaeX aa well as an aphid Toxoptera aurantii (Hemiptera: Aphidoidea: Aphididae). The first tial on disease transmission by using P. minor, previously reared on the potato tubers, showed one out of ten tested plants produced disease sympom. The subsequent trial using P minor, bred on healthy black pepper seedlings, showed thee out often tested plants developed disease symptoms. None of the aphid transmitted plants developed Ihe disease. This study confirmed thai PYMV b the cause of stunting disease on black pepper in IP Sukamulya and Lampung and P. minor as the insect vecor of the disease.

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