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SERANGAN Mimegralla coeruleifrons Macquart PADA TANAMAN JAHE DAN PERANANNYA DALAM MEMBAWA PATOGEN PENYAKIT LAYU RODIAH BALFAS; SUPRIADI SUPRIADI; NURI KARYANI; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.123-127

Abstract

The attack of Mimegralla coeruleifrons on ginger plants and its role in carrying pathogens of wilt diseaseRhizome flics Mimegralla coeruleifrons (Diplera; Micropezidae) ac frequently oten found in the diseased ginger rhizome caused by Ralstonia solanacearum, bill it has nol been known which one made the irst damage. Experiments were conducted at laboratory and green house of the Research Institute for Spice and Medicinal Cops from September 1998 to I'cbruary 2000. to examine the attack of M coerulefrons on healthy and disease ginger plants and its role in carrying the pauiogen. Hie experiments consisted of three parts, ink-station of the fly in healthy and diseased ginger plants, disease tansmission and detection of R solanacearum in M coeruleifrons larvae and adults, fiingcr plants were grown in pot containing, sterile media The pots were placed in the field and the plants were inoculated with R solanacearum and iininoculatcd. then they were caged or uncaged. Adult lies (33 - 35 pairs) were introduced into the cage. The uncaged plants were allowed to be naturally invested by the flies. Disease transmission used adult Hies which were collected from diseased ginger plantation. The lies were fed with suspension of R solanacearum. then introduced into cages containing healthy ginger plants. The flies were also directly caged on healthy plants Detection of/?, solanacearum in larvae and adults was carried out by isolation on selective medium and ELISA techniques. Results showed that M coerulefrons mostly attacked the inoculated ginger plants. Tliere was no evidence of Iransmission of R solanacearum by the fly. whereas nearly 50% of the ield collected lics carried R solanacearum. These results tend to suggest that the attack of M. coerulerons occured ater the ginger has been infected by wilt disease and the ly can be a carrier of/? solanacearum.
PENYEBAB DAN SERANGGA VEKTOR PENYAKIT KERITING PADA TANAMAN LADA RODIAH BALFAS; SUPRIADI SUPRIADI; T. L. MARDININGSIH; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 8, No 1 (2002): Maret, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n1.2002.7-11

Abstract

The cause of stunting disease of black pepper (Piper nigrum) in Indonesia has not yet been confirmed cither due o a virus or mycoplaam. However, similar disease found on black pepper plants in the Southeast Asia is caused by Piper yellow mottle virus (PYMV) which is transmitted by Planococcus citri. This expeiments was aimed o examine the cause of the stunting disease and its insect vectors. The expeiment were conducted from October 1998 to May 2001. Diseased vegetative materials of black pepper plants showing stunting disease were collected from Sukamulya, Sukabumi. The potential insect vectors were collected from black pepper plants in Bogor, IP Sukamulya (Sukabumi), Lampung and Bangka. The insects were fed on the diseased plants obtained from Lampung, Bogor and IP Sukamulya, then transferred o the healthy plants. The healthy plants were produced rom true seeds and cutings oiginated rom Bogor and IP Sukamulya. The tested plants were incubated at the green house and examined for disease development. Diseased leaf samples collected rom black pepper plant rom IP. Sukamulya and the transmitted plants were sent to the University of Minnesota, USA for Ihe virus (PYMV) by using ISEM (immunosorbent electron microscope). The result snowed that the leaves samples rom IP. Sukamulya were infected by PYMV. The morphology and size of the virus were similar to those caused stunting disease in the South East Asia. Potential insects vectors found on the diseased black pepper plants were two mealybugs, P. minor and Ferrisia virgata (Hemiptera; Coccoidca: PaaidbcoccidaeX aa well as an aphid Toxoptera aurantii (Hemiptera: Aphidoidea: Aphididae). The first tial on disease transmission by using P. minor, previously reared on the potato tubers, showed one out of ten tested plants produced disease sympom. The subsequent trial using P minor, bred on healthy black pepper seedlings, showed thee out often tested plants developed disease symptoms. None of the aphid transmitted plants developed Ihe disease. This study confirmed thai PYMV b the cause of stunting disease on black pepper in IP Sukamulya and Lampung and P. minor as the insect vecor of the disease.
SERANGAN Mimegralla coeruleifrons Macquart PADA TANAMAN JAHE DAN PERANANNYA DALAM MEMBAWA PATOGEN PENYAKIT LAYU RODIAH BALFAS; SUPRIADI SUPRIADI; NURI KARYANI; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.123-127

Abstract

The attack of Mimegralla coeruleifrons on ginger plants and its role in carrying pathogens of wilt diseaseRhizome flics Mimegralla coeruleifrons (Diplera; Micropezidae) ac frequently oten found in the diseased ginger rhizome caused by Ralstonia solanacearum, bill it has nol been known which one made the irst damage. Experiments were conducted at laboratory and green house of the Research Institute for Spice and Medicinal Cops from September 1998 to I'cbruary 2000. to examine the attack of M coerulefrons on healthy and disease ginger plants and its role in carrying the pauiogen. Hie experiments consisted of three parts, ink-station of the fly in healthy and diseased ginger plants, disease tansmission and detection of R solanacearum in M coeruleifrons larvae and adults, fiingcr plants were grown in pot containing, sterile media The pots were placed in the field and the plants were inoculated with R solanacearum and iininoculatcd. then they were caged or uncaged. Adult lies (33 - 35 pairs) were introduced into the cage. The uncaged plants were allowed to be naturally invested by the flies. Disease transmission used adult Hies which were collected from diseased ginger plantation. The lies were fed with suspension of R solanacearum. then introduced into cages containing healthy ginger plants. The flies were also directly caged on healthy plants Detection of/?, solanacearum in larvae and adults was carried out by isolation on selective medium and ELISA techniques. Results showed that M coerulefrons mostly attacked the inoculated ginger plants. Tliere was no evidence of Iransmission of R solanacearum by the fly. whereas nearly 50% of the ield collected lics carried R solanacearum. These results tend to suggest that the attack of M. coerulerons occured ater the ginger has been infected by wilt disease and the ly can be a carrier of/? solanacearum.
PENYEBAB DAN SERANGGA VEKTOR PENYAKIT KERITING PADA TANAMAN LADA RODIAH BALFAS; SUPRIADI SUPRIADI; T. L. MARDININGSIH; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 8, No 1 (2002): Maret, 2002
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v8n1.2002.7-11

Abstract

The cause of stunting disease of black pepper (Piper nigrum) in Indonesia has not yet been confirmed cither due o a virus or mycoplaam. However, similar disease found on black pepper plants in the Southeast Asia is caused by Piper yellow mottle virus (PYMV) which is transmitted by Planococcus citri. This expeiments was aimed o examine the cause of the stunting disease and its insect vectors. The expeiment were conducted from October 1998 to May 2001. Diseased vegetative materials of black pepper plants showing stunting disease were collected from Sukamulya, Sukabumi. The potential insect vectors were collected from black pepper plants in Bogor, IP Sukamulya (Sukabumi), Lampung and Bangka. The insects were fed on the diseased plants obtained from Lampung, Bogor and IP Sukamulya, then transferred o the healthy plants. The healthy plants were produced rom true seeds and cutings oiginated rom Bogor and IP Sukamulya. The tested plants were incubated at the green house and examined for disease development. Diseased leaf samples collected rom black pepper plant rom IP. Sukamulya and the transmitted plants were sent to the University of Minnesota, USA for Ihe virus (PYMV) by using ISEM (immunosorbent electron microscope). The result snowed that the leaves samples rom IP. Sukamulya were infected by PYMV. The morphology and size of the virus were similar to those caused stunting disease in the South East Asia. Potential insects vectors found on the diseased black pepper plants were two mealybugs, P. minor and Ferrisia virgata (Hemiptera; Coccoidca: PaaidbcoccidaeX aa well as an aphid Toxoptera aurantii (Hemiptera: Aphidoidea: Aphididae). The first tial on disease transmission by using P. minor, previously reared on the potato tubers, showed one out of ten tested plants produced disease sympom. The subsequent trial using P minor, bred on healthy black pepper seedlings, showed thee out often tested plants developed disease symptoms. None of the aphid transmitted plants developed Ihe disease. This study confirmed thai PYMV b the cause of stunting disease on black pepper in IP Sukamulya and Lampung and P. minor as the insect vecor of the disease.