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All Journal Jurnal Penelitian Tanaman Industri Buletin Penelitian Tanaman Rempah dan Obat Jurnal Penelitian Tanaman Industri (Littri) Buletin Penelitian Tanaman Rempah dan Obat
NURI KARYANI
Balai Penelitian Tanaman Rempah dan Obat
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Journal : Jurnal Penelitian Tanaman Industri (Littri)

 1 
SERANGAN Mimegralla coeruleifrons Macquart PADA TANAMAN JAHE DAN PERANANNYA DALAM MEMBAWA PATOGEN PENYAKIT LAYU RODIAH BALFAS; SUPRIADI SUPRIADI; NURI KARYANI; ENDANG SUGANDI
Jurnal Penelitian Tanaman Industri Vol 5, No 4 (2000): Maret, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/littri.v5n4.2000.123-127

Abstract

The attack of Mimegralla coeruleifrons on ginger plants and its role in carrying pathogens of wilt diseaseRhizome flics Mimegralla coeruleifrons (Diplera; Micropezidae) ac frequently oten found in the diseased ginger rhizome caused by Ralstonia solanacearum, bill it has nol been known which one made the irst damage. Experiments were conducted at laboratory and green house of the Research Institute for Spice and Medicinal Cops from September 1998 to I'cbruary 2000. to examine the attack of M coerulefrons on healthy and disease ginger plants and its role in carrying the pauiogen. Hie experiments consisted of three parts, ink-station of the fly in healthy and diseased ginger plants, disease tansmission and detection of R solanacearum in M coeruleifrons larvae and adults, fiingcr plants were grown in pot containing, sterile media The pots were placed in the field and the plants were inoculated with R solanacearum and iininoculatcd. then they were caged or uncaged. Adult lies (33 - 35 pairs) were introduced into the cage. The uncaged plants were allowed to be naturally invested by the flies. Disease transmission used adult Hies which were collected from diseased ginger plantation. The lies were fed with suspension of R solanacearum. then introduced into cages containing healthy ginger plants. The flies were also directly caged on healthy plants Detection of/?, solanacearum in larvae and adults was carried out by isolation on selective medium and ELISA techniques. Results showed that M coerulefrons mostly attacked the inoculated ginger plants. Tliere was no evidence of Iransmission of R solanacearum by the fly. whereas nearly 50% of the ield collected lics carried R solanacearum. These results tend to suggest that the attack of M. coerulerons occured ater the ginger has been infected by wilt disease and the ly can be a carrier of/? solanacearum.
Pestalotiopsis desseminata PADA JAMBU MENTE BIOLOGI DAN INTERAKSINYA DENGAN Helopeltis antonii ESTHER M. ADHI; SUPRIADI SUPRIADI; S. RAHAYUNINGSIH; D. KILIN; NURI KARYANI
Jurnal Penelitian Tanaman Industri Vol 6, No 3 (2000): Desember, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n3.2000.66-72

Abstract

Pestalotiopsis desseminata on cashew: its biology and interaction with Helopeltis antoniiPestalotiopsis desseminata is one of pathogens causing leaf spots. die-back of shoots and inflorescence of cashew plant. It is assumed there is an interaction between the attack of Helopeltis antonii and the fungus in Ihe ield so that the damage becomes more devastating. The objectives of (his research were to investigate several aspects of fungal biology (pathogenicity, mycelial growth, acervuli production, sensitivity to fungicides) and its interaction with //. antonii. The esearch was carried out from April 1999 to March 2000 at the laboratory and green house of Pest and Disease Department Research Institute for Spice and Medicinal Crops, Bogor. /' desseminata was diectly isolated from cashew leaves. Pathogenicity test of P. desseminata isolate was conducted on cashew seedlings. The fungal growth and fungicide effects were carried out in vitro on agar medium. The interaction between P. desseminata and H. antonii was examined by inoculating the fungus and insect, either individually or in combination, on cashew seedlings. The results showed that P. desseminata isolate fomis black spherical acervuli containing oblongs conidia with 5 cells, and the outermost cell has 3 flagellate. Acervuli could only be produced on the cultue illuminated continuously by 600 lux translucent lamp. Pathogenicity test of several isolates of /' desseminata on cashew seedlings caused spherical leaf spot symptom, individually or coalesced as bigger leaf spots. In vitro fungal mycelia growth could be suppressed by several fungicides, including bcnomyl 50% (I ppm) and Uiiopanate-mcthyl 70% (10 ppm) Combining inoculation between P. desseminata and //. antonii resulted in moe severe (devastating) die-back compared with individually inoculated.
POTENSI BAKTERI ANTAGONIS DALAM MENEKAN PERKEMBANGAN PENYAKIT LAYU BAKTERI JAHE KARDEN MULYA; SUPRIADI SUPRIADI; ESTHER M. ADHI; SRI RAHAYU; NURI KARYANI
Jurnal Penelitian Tanaman Industri Vol 6, No 2 (2000): September, 2000
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jlittri.v6n2.2000.37-43

Abstract

Potency of antagonist bacteria in inhibiting the bacterial wilt disease progress gingerBacterial wilt disease caused by Pseudomonas solanacearum is an important disease in ginger plant (Zingiber oicinale Rose.) in Indonesia. The objective of this research was to study the effectiveness of biological agents in inhibiting the progess of bacterial wilt on ginger. The research was conducted at the geenhouse of Research Institute for Spice and Medicinal Crops in 1997-1998 then was followed with a ield experiment at Sukamulya Experimental Garden in 1998/1999. At the greenhouse experiment seven kinds of antagonists bacteria were formulated either individually or combination. Pseudomonas fluorescens (PF), P. cepacia (PC) and Bacillus sp. (BC), mixtue of PF + PC, mixtue of PC + BC, mixture of PF + BC, and mixtue of PF + PC + BC were tested in the geenhouse on ginger plant cultivars putih besar grown in the pot containing soil formerly used for growing P. solanacearum infected tomato. I he results of this experiment indicated that the combination of PF+PC+BC significantly suppressed the progress of bacterial wilt disease compaed to control and other tested combination. In the ield experiment carried out at the bacterial wilt disease endemic area the combinations of PF+PC+BC, Trichoderma harzianum (Bit-1) and combination of PF+PC+BC+BII-I were tested with two levels of application, i.e. two applications with two months interval and 4 application with one month interval. The results showed that the application of antagonists bacteria inhibited Ihe bacterial wilt disease progress and signiicantly increased ginger rhizome yield. The yield of the rhizome from the plants teated with different intervals were not different. However, the application of the antagonist bacteria were not able to eradicate ginger infection by Pseudomonas solanacearum thoroughly.
Co-Authors D. KILIN ENDANG SUGANDI ESTHER M. ADHI KARDEN MULYA RODIAH BALFAS S. RAHAYUNINGSIH SRI RAHAYU Sri Yuni Hartati SUPRIADI SUPRIADI