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Meynarti Sari Dewi Ibrahim
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Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management

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PENGARUH PEMBERIAN PACLOBUTRAZOL TERHADAP PERTUMBUHAN BANGLE (Zingiber purpureum Roxb) DALAM PENYIMPANAN IN –VITRO Meynarti Sari Dewi Ibrahim
Buletin Penelitian Tanaman Rempah dan Obat Vol 16, No 2 (2005): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v16n2.2005.%p

Abstract

Effect of Paclobutrazol On The Growth of In Vitro Concerved Purple Ginger (Zingiber purpureum Roxb)Effect of paclobutrazol on the growth of Purple Ginger (Zingiber purpureum  Roxburgh) conserved in vitro were studied in the Laboratory of Breeding and gremplasm of Indonesian Spice and Medicinal Crops Research Instituted, from February to November 2003. Sterile shoots of Purple Ginger were used as explants sources. The treatments evaluated were paclobutrazol 0 (control); 1; 2; 3 mg/l and arranged in completely randomized design with 10 replications. The aim of research was to study the effect of paclobutrazol on the growth of Purple Ginger conserved in vitro.  Results showed that at 5 months of culture age, paclobutrazol significantly reduced the number of shoots, height number of leaves and number of roots. The higher the concentration of paclobutrazol, the lower the number of shoots, leaves and roots and the shorter the root of plantlets observed. Application of paclobutrazol at concentration of 2 or 3 mg/l could prolong the conservation period up to 9 months. Fur there more it was revealed that paclobutrazol application produced more vigorous plantlets. 
STUDI PENDAHULUAN : INDUKSI KALUS EMBRIOGENIK DARI EKSPLAN DAUN Echinaceae purpurea Meynarti Sari Dewi Ibrahim; N. Nova Kristina; Nurliani Bermawie
Buletin Penelitian Tanaman Rempah dan Obat Vol 15, No 2 (2004): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v15n2.2004.%p

Abstract

Echinaceae is an introduced medicinal plant used to improve immune system of the body. Lately, interest on Echinaceae increased, however, good plant material for development is limited. One method to multiply plant material is the in vitro culture, so that research and development especially in vitro multiplication is required. The aim of the experiment is to procure a medium for the induction of embryonic callus and the technique of regeneration. The experiment is conducted at the Laboratory of Breeding and Genetic Resources, ISMECRI from January to December 2002, consisted of 2 steps, namely(1). Embryonic callus induction, (2). Callus regeneration. In the first step, explants were transferred into MS medium enrich with BA 0,1 mg/l + 2,4 D 0,5 mg/l and a combination medium of MS and LS medium supplemented with glutamine + BA (0,1 mg/l; 0,2 mg/l) + 2,4 D (0,5mg/l ; 1 mg/l). In the second step, regeneration was conducted on medium MS combined with LS + BA (0 mg/l + 0,2 mg/l + 0,4 mg/l), MS combined with LS + Kinetine (0,2 mg/l; 0,4 mg/l). The results showed that embryonic callus was obtained from culture of leaf explants on MS medium combined with LS. The best treatment for step regeneration was MS combined LS + BA 0,4 mg/l for shoot formation while MS combined LS + kinetine 0,4 mg/l induced root formation. 
PENGARUH PEMBERIAN PACLOBUTRAZOL TERHADAP PERTUMBUHAN BANGLE (Zingiber purpureum Roxb) DALAM PENYIMPANAN IN –VITRO Meynarti Sari Dewi Ibrahim
Buletin Penelitian Tanaman Rempah dan Obat Vol 16, No 2 (2005): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v16n2.2005.%p

Abstract

Effect of Paclobutrazol On The Growth of In Vitro Concerved Purple Ginger (Zingiber purpureum Roxb)Effect of paclobutrazol on the growth of Purple Ginger (Zingiber purpureum  Roxburgh) conserved in vitro were studied in the Laboratory of Breeding and gremplasm of Indonesian Spice and Medicinal Crops Research Instituted, from February to November 2003. Sterile shoots of Purple Ginger were used as explants sources. The treatments evaluated were paclobutrazol 0 (control); 1; 2; 3 mg/l and arranged in completely randomized design with 10 replications. The aim of research was to study the effect of paclobutrazol on the growth of Purple Ginger conserved in vitro.  Results showed that at 5 months of culture age, paclobutrazol significantly reduced the number of shoots, height number of leaves and number of roots. The higher the concentration of paclobutrazol, the lower the number of shoots, leaves and roots and the shorter the root of plantlets observed. Application of paclobutrazol at concentration of 2 or 3 mg/l could prolong the conservation period up to 9 months. Fur there more it was revealed that paclobutrazol application produced more vigorous plantlets. 
Co-Authors N. Nova Kristina Nurliani Bermawie