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All Journal Buletin Penelitian Tanaman Rempah dan Obat Jurnal Ilmu Ternak Veteriner Buletin Penelitian Tanaman Rempah dan Obat
N. Bermawie
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Agriculture, Biological Sciences & Forestry Decision Sciences, Operations Research & Management Veterinary

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SKRINING ISSR PRIMER STUDI PENDAHULUAN KEKERABATAN ANTAR JAHE MERAH, JAHE EMPRIT DAN JAHE BESAR Sri Wahyuni; D. H. Xu2; N. Bermawie; H. Tsunematsu; T. Ban
Buletin Penelitian Tanaman Rempah dan Obat Vol 15, No 1 (2004): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v15n1.2004.%p

Abstract

Screening ISSR primer preliminary study of the relationship between small, big and red gingerScreening ISSR Primer as preliminary study the relationships of small, big and red ginger. A PCR- based method for genetic diversity analysis such as ISSR has been well developed recently. In this research, 28 ISSR primer were screened aiming to study the relationships of red, small and big ginger. Genomic DNA was isolated from rhizome using CTAB based method. The DNA were then amplified in a thermalcycler wite a total volume of 20 µl consisted of 30 – 100 ng genomic DNA, 10 pmol primer, 1x PCR buffer, 0,2 mM dNTPs, 1,5 mM MgCl2 dan 0,5 rTaq polymerase. Electroforesis of the amplification product was carried out on 1,5% agarose gel, stained with ethidium bromide and viewing on UV transiluminator light to see the banding pattern. Results showed that amplified banding pattern of ISSR range from 3 – 14. Among the 28 primers screened, 10 primers showed polymorphic banding pattern but only 3 primers showed polymorphic banas more than 25%, i. e. ISSR 18, ISSR 19, and ISSR 15 respectively. Based on the ISSR data, small and big ginger has closer relationships compared to the red ginger. To find the suitable primers that can be used to distinguished the ginger cultivar and find suitable molecular markers, more primers need to be screened. 
The Utilization of turmeric and curcuma xanthorrhiza as feed additive for broilers A.P. Sinurat; T. Purwadaria; I.A.K. Bintang; P.P. Ketaren; N. Bermawie; M. Raharjo; M. Rizal
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 2 (2009): JUNE 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (68.877 KB) | DOI: 10.14334/jitv.v14i2.349

Abstract

The use of plant bioactives to replace antibiotics are now widely investigated. Turmeric or Curcuma longa (CL) and curcuma xanthorrhiza (CX), are commonly used by human and known to have active ingredients as antimicrobial. Therefore a research was conducted to evaluate the possibility of using these plant bioactives to replace antibiotic in poultry feed. The bioactives concentration of the CL and CX powder were measured prior to the feeding trial and then supplemented into standard diets of broiler chikens. The levels tested in this trial were based on the active ingredients that could inhibit growth of bacteria and fungi, i.e., low, medium and high levels of the CL and CX, respectively. The combination of low level of CL + high level of CX and low level CL +  medium level of CX were also tested. A diet without feed additives and with antibiotics were used as controls. Each diet was fed from day old to 35 days old, replicated 6 times and each replication consist of 15 birds. Results showed that neither the antibiotic tested nor the turmeric (CL), xanthorrhiza (CX) nor the mixture of CL and CX gave significant (P>0.05) improvement on performances (body weight, FCR and mortatlity), nutrient digestibility of feed and carcass yield of broilers. Key Words:  Broilers, Curcuma Longa, Curcuma Canthorrizhol, Bioactive
SKRINING ISSR PRIMER STUDI PENDAHULUAN KEKERABATAN ANTAR JAHE MERAH, JAHE EMPRIT DAN JAHE BESAR Sri Wahyuni; D. H. Xu2; N. Bermawie; H. Tsunematsu; T. Ban
Buletin Penelitian Tanaman Rempah dan Obat Vol 15, No 1 (2004): BULETIN PENELITIAN TANAMAN REMPAH DAN OBAT
Publisher : Pusat Penelitian dan Pengembangan Perkebunan

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/bullittro.v15n1.2004.%p

Abstract

Screening ISSR primer preliminary study of the relationship between small, big and red gingerScreening ISSR Primer as preliminary study the relationships of small, big and red ginger. A PCR- based method for genetic diversity analysis such as ISSR has been well developed recently. In this research, 28 ISSR primer were screened aiming to study the relationships of red, small and big ginger. Genomic DNA was isolated from rhizome using CTAB based method. The DNA were then amplified in a thermalcycler wite a total volume of 20 µl consisted of 30 – 100 ng genomic DNA, 10 pmol primer, 1x PCR buffer, 0,2 mM dNTPs, 1,5 mM MgCl2 dan 0,5 rTaq polymerase. Electroforesis of the amplification product was carried out on 1,5% agarose gel, stained with ethidium bromide and viewing on UV transiluminator light to see the banding pattern. Results showed that amplified banding pattern of ISSR range from 3 – 14. Among the 28 primers screened, 10 primers showed polymorphic banding pattern but only 3 primers showed polymorphic banas more than 25%, i. e. ISSR 18, ISSR 19, and ISSR 15 respectively. Based on the ISSR data, small and big ginger has closer relationships compared to the red ginger. To find the suitable primers that can be used to distinguished the ginger cultivar and find suitable molecular markers, more primers need to be screened. 
Co-Authors A.P. Sinurat D. H. Xu2 H. Tsunematsu I.A.K. Bintang M. Raharjo M. Rizal P.P. Ketaren Sri Wahyuni T. Ban T. Purwadaria