Garuda - Garba Rujukan Digital

Mikropropagasi Pada Tanaman Stevia rebaudiana (Bertoni) Parnidi Parnidi; Aprilia Ridhawati
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 12, No 1 (2020): APRIL 2020
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/btsm.v12n1.2020.45-53

Stevia merupakan salah satu tanaman penghasil pemanis alami. Mikropropagasi stevia melalui kultur jaringan dapat menyediakan bahan tanaman secara massal dan cepat yang diperlukan untuk pengembangan stevia. Pada mikropopagasi melalui kultur jaringan diperlukan komposisi media yang tepat. Penelitian ini bertujuan untuk menguji komposisi media yang sesuai untuk mikropropagasi tanaman stevia. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat pada Februari - Juni 2016. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) setiap perlakuan diulang tiga kali. Induksi tunas stevia menggunakan media dasar Murashige and Skoog (MS) dengan penambahan Benzil Amino Purin (BAP) dengan konsentrasi 0; 0,25; 0,5; 0,75 dan 1 mg/L. Induksi perakaran stevia menggunakan media dasar MS dengan dengan penambahan 1; 1,5; 2 dan 2,5 mg/L IAA, IBA dan NAA dan media MS tanpa penambahan ZPT sebagai kontrol. Hasil penelitian menunjukkan bahwa media MS + BAP 0,5 mg/L menunjukkan pertumbuhan terbaik dengan rerata jumlah tunas 17,80 dan rerata panjang tunas 3,25 cm. Media perakaran terbaik terdapat pada perlakuan media MS + IAA 1mg/L yang menghasilkan jumlah akar dengan rerata 4,60 dan panjang akar 2,27 cm.ABSTRACTMicropropagation of Stevia rebaudiana (Bertoni)Stevia is one of the plants that produces natural sweeteners. Stevia micropropagation through tissue culture can provide a large of amount and fast plant material needed for stevia plantation. Micropropagation through tissue culture requires a proper media composition. This study aims to examine the composition of media suitable for stevia micropropagation. The study was conducted at the Tissue Culture Laboratory, Indonesian Sweeteners and Fiber Crops Research Institute in February - June 2016; using a completely randomized design (CRD) with three replicates. The treatment for shoot induction using Murashige and Skoog (MS) basic medium plus Benzyl Amino Purin (BAP) with concentrations: 0; 0.25; 0.5; 0.75 and 1 mg/L. The treatment for root induction using MS basic medium with the addition of 1; 1,5; 2 and 2,5 mg/L IAA, IBA and NAA and for control using MS basic medium without the addition of plant growth regulators. The results showed that the best growth of stevia shoots with mean of shoots number 17.80 and mean of shoots length 3.25 cm was found in MS basic medium + BAP 0.5 mg/L. The best stevia root growth with mean of root number 4.60 and mean of root length 2.27 cm was found in MS basic medium + IAA 1 mg/L.

Pengaruh Komposisi Media Terhadap Induksi Tunas dan Akar Lima Genotipe Tanaman Agave Pada Kultur In Vitro Aprilia Ridhawati; Tantri Dyah Ayu Anggraeni; Rully Dyah Purwati
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 9, No 1 (2017): April 2017
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/btsm.v9n1.2017.1-9

Agave (Agave sisalana Perrine) merupakan tanaman penghasil serat alam. Pengembangan agave terkendala penyediaan bahan tanam bermutu. Teknik kultur jaringan dapat menghasilkan benih agave dalam jumlah banyak dengan kualitas yang seragam. Tujuan penelitian adalah untuk mendapatkan komposisi media terbaik dalam induksi tunas dan akar lima genotipe agave pada kultur in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Balittas dari bulan Juli 2015 sampai Juni 2016. Sumber eksplan adalah tunas aseptik agave genotipe Balittas 10, Balittas 12, Balittas 13, Balittas 14, dan H-11648 dari kultur in vitro. Rancangan penelitian yang digunakan rancangan acak lengkap faktorial (dua faktor, tiga ulangan). Faktor I adalah komposisi media dan faktor II adalah genotipe. Komposisi media induksi tunas: M1 (MS + BAP 0,5 mg/l + IBA 0,5 mg/l); M2 (MS + BAP 1 mg/l + IBA 0,5 mg/l), dan M3 (MS + BAP 1,5 mg/l + IBA 0,5 mg/l). Komposisi media perakaran: M1 (MS + arang aktif 2 g/l); M2 (MS + arang aktif 2 g/l + IBA 0,5 mg/l); M3 (MS + arang aktif 2 g/l + IBA 1 g/l); M4 (MS + arang aktif 2 g/l + NAA 0,5 mg/l), dan M5 (MS + arang aktif 2 g/l + NAA 1 mg/l). Hasil penelitian menunjukkan komposisi media induksi tunas menghasilkan jumlah tunas (1,09–1,33) dan kecepatan induksi (4 minngu) yang tidak berbeda nyata. Komposisi media induksi akar yang terbaik adalah media M4 (MS + arang aktif 2 g/l + NAA 0,5 mg/l) dengan jumlah akar 4,53. Genotipe Balittas 14 menghasilkan jumlah tunas dan jumlah akar yang paling tinggi dibandingkan genotipe lain (1,56 tunas dan 4,59 akar). The Effect of Media Composition on The Induction of Shoot and Roots and of Five Agave Clones on In Vitro CultureAgave (Agave sisalana Perrine) is a plant that producenaturalfibre. Agave cultivation for commercial use is still limited by the availability of good plant materials. In vitro culture technique can produce a large amount of plant material with same quality in relatively short time. The study aimed to obtain a suitable medium composition for in vitro shoot multiplication and root induction for five agave genotypes. The experiment was conducted from July 2015 to June 2016 in Tissue Culture Laboratory of Indonesian Sweetener and Fibre Crops Research Institute. Explant source derived from aseptic shoot of agave genotypesBalittas 10, 12, 13, 14, andH-11648 in in vitro ISFCRI germplasm collection. The experiment was arranged in factorial complete random design (two factors: media composition, genotype, and three replication). Shoot induction media: M1 (MS + BAP 0.5 mg/l + IBA 0.5 mg/l); M2 (MS + BAP 1 mg/l + IBA 0.5 mg/l);and M3 (MS + BAP 1.5 mg/l + IBA 0.5 mg/l). Root induction media: M1 (MS + active carbon(AC) 2 g/l); M2 (MS + AC2 g/l + IBA 0,5 mg/l); M3 (MS + AC 2 g/l + IBA 1 g/l); M4 (MS + AC 2 g/l + NAA 0,5 mg/l);and M5 (MS + AC 2 g/l + NAA 1 mg/l). The results showed that the shoot induction media compositions were not differ significantly on shoot numbers (1.09–1.33) and time for shoot induction (4 weeks). The best composition medium of root induction was M4 (MS + AC 2 g/l + NAA 0.5 mg/l), that yielded 4.53 root numbers. Balittas 14 genotype yielded the highest shoot and root numbers (1,56 shoot numbers and 4.59 root numbers).

Pengaruh Komposisi Media Terhadap Induksi Tunas dan Akar Lima Genotipe Tanaman Agave Pada Kultur In Vitro Aprilia Ridhawati; Tantri Dyah Ayu Anggraeni; Rully Dyah Purwati
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 9, No 1 (2017): April 2017
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Agave (Agave sisalana Perrine) merupakan tanaman penghasil serat alam. Pengembangan agave terkendala penyediaan bahan tanam bermutu. Teknik kultur jaringan dapat menghasilkan benih agave dalam jumlah banyak dengan kualitas yang seragam. Tujuan penelitian adalah untuk mendapatkan komposisi media terbaik dalam induksi tunas dan akar lima genotipe agave pada kultur in vitro. Penelitian dilaksanakan di Laboratorium Kultur Jaringan Balittas dari bulan Juli 2015 sampai Juni 2016. Sumber eksplan adalah tunas aseptik agave genotipe Balittas 10, Balittas 12, Balittas 13, Balittas 14, dan H-11648 dari kultur in vitro. Rancangan penelitian yang digunakan rancangan acak lengkap faktorial (dua faktor, tiga ulangan). Faktor I adalah komposisi media dan faktor II adalah genotipe. Komposisi media induksi tunas: M1 (MS + BAP 0,5 mg/l + IBA 0,5 mg/l); M2 (MS + BAP 1 mg/l + IBA 0,5 mg/l), dan M3 (MS + BAP 1,5 mg/l + IBA 0,5 mg/l). Komposisi media perakaran: M1 (MS + arang aktif 2 g/l); M2 (MS + arang aktif 2 g/l + IBA 0,5 mg/l); M3 (MS + arang aktif 2 g/l + IBA 1 g/l); M4 (MS + arang aktif 2 g/l + NAA 0,5 mg/l), dan M5 (MS + arang aktif 2 g/l + NAA 1 mg/l). Hasil penelitian menunjukkan komposisi media induksi tunas menghasilkan jumlah tunas (1,09–1,33) dan kecepatan induksi (4 minngu) yang tidak berbeda nyata. Komposisi media induksi akar yang terbaik adalah media M4 (MS + arang aktif 2 g/l + NAA 0,5 mg/l) dengan jumlah akar 4,53. Genotipe Balittas 14 menghasilkan jumlah tunas dan jumlah akar yang paling tinggi dibandingkan genotipe lain (1,56 tunas dan 4,59 akar). The Effect of Media Composition on The Induction of Shoot and Roots and of Five Agave Clones on In Vitro CultureAgave (Agave sisalana Perrine) is a plant that producenaturalfibre. Agave cultivation for commercial use is still limited by the availability of good plant materials. In vitro culture technique can produce a large amount of plant material with same quality in relatively short time. The study aimed to obtain a suitable medium composition for in vitro shoot multiplication and root induction for five agave genotypes. The experiment was conducted from July 2015 to June 2016 in Tissue Culture Laboratory of Indonesian Sweetener and Fibre Crops Research Institute. Explant source derived from aseptic shoot of agave genotypesBalittas 10, 12, 13, 14, andH-11648 in in vitro ISFCRI germplasm collection. The experiment was arranged in factorial complete random design (two factors: media composition, genotype, and three replication). Shoot induction media: M1 (MS + BAP 0.5 mg/l + IBA 0.5 mg/l); M2 (MS + BAP 1 mg/l + IBA 0.5 mg/l);and M3 (MS + BAP 1.5 mg/l + IBA 0.5 mg/l). Root induction media: M1 (MS + active carbon(AC) 2 g/l); M2 (MS + AC2 g/l + IBA 0,5 mg/l); M3 (MS + AC 2 g/l + IBA 1 g/l); M4 (MS + AC 2 g/l + NAA 0,5 mg/l);and M5 (MS + AC 2 g/l + NAA 1 mg/l). The results showed that the shoot induction media compositions were not differ significantly on shoot numbers (1.09–1.33) and time for shoot induction (4 weeks). The best composition medium of root induction was M4 (MS + AC 2 g/l + NAA 0.5 mg/l), that yielded 4.53 root numbers. Balittas 14 genotype yielded the highest shoot and root numbers (1,56 shoot numbers and 4.59 root numbers).

Mikropropagasi Pada Tanaman Stevia rebaudiana (Bertoni) Parnidi Parnidi; Aprilia Ridhawati
Buletin Tanaman Tembakau, Serat & Minyak Industri Vol 12, No 1 (2020): APRIL 2020
Publisher : Balai Penelitian Tanaman Pemanis dan Serat

Stevia merupakan salah satu tanaman penghasil pemanis alami. Mikropropagasi stevia melalui kultur jaringan dapat menyediakan bahan tanaman secara massal dan cepat yang diperlukan untuk pengembangan stevia. Pada mikropopagasi melalui kultur jaringan diperlukan komposisi media yang tepat. Penelitian ini bertujuan untuk menguji komposisi media yang sesuai untuk mikropropagasi tanaman stevia. Penelitian dilakukan di Laboratorium Kultur Jaringan, Balai Penelitian Tanaman Pemanis dan Serat pada Februari - Juni 2016. Penelitian dilakukan menggunakan Rancangan Acak Lengkap (RAL) setiap perlakuan diulang tiga kali. Induksi tunas stevia menggunakan media dasar Murashige and Skoog (MS) dengan penambahan Benzil Amino Purin (BAP) dengan konsentrasi 0; 0,25; 0,5; 0,75 dan 1 mg/L. Induksi perakaran stevia menggunakan media dasar MS dengan dengan penambahan 1; 1,5; 2 dan 2,5 mg/L IAA, IBA dan NAA dan media MS tanpa penambahan ZPT sebagai kontrol. Hasil penelitian menunjukkan bahwa media MS + BAP 0,5 mg/L menunjukkan pertumbuhan terbaik dengan rerata jumlah tunas 17,80 dan rerata panjang tunas 3,25 cm. Media perakaran terbaik terdapat pada perlakuan media MS + IAA 1mg/L yang menghasilkan jumlah akar dengan rerata 4,60 dan panjang akar 2,27 cm.ABSTRACTMicropropagation of Stevia rebaudiana (Bertoni)Stevia is one of the plants that produces natural sweeteners. Stevia micropropagation through tissue culture can provide a large of amount and fast plant material needed for stevia plantation. Micropropagation through tissue culture requires a proper media composition. This study aims to examine the composition of media suitable for stevia micropropagation. The study was conducted at the Tissue Culture Laboratory, Indonesian Sweeteners and Fiber Crops Research Institute in February - June 2016; using a completely randomized design (CRD) with three replicates. The treatment for shoot induction using Murashige and Skoog (MS) basic medium plus Benzyl Amino Purin (BAP) with concentrations: 0; 0.25; 0.5; 0.75 and 1 mg/L. The treatment for root induction using MS basic medium with the addition of 1; 1,5; 2 and 2,5 mg/L IAA, IBA and NAA and for control using MS basic medium without the addition of plant growth regulators. The results showed that the best growth of stevia shoots with mean of shoots number 17.80 and mean of shoots length 3.25 cm was found in MS basic medium + BAP 0.5 mg/L. The best stevia root growth with mean of root number 4.60 and mean of root length 2.27 cm was found in MS basic medium + IAA 1 mg/L.

The Vigor and Viability Seed Testing of Three Tobacco Varieties on Various Seed Germination Media Taufiq Hidayat RS; Aprilia Ridhawati
Agrotech Journal Vol 5, No 1 (2020): Agrotech Journal
Publisher : Universitas Sembilanbelas November Kolaka

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.31327/atj.v5i1.1210

Seed viability is one of the most important physiological quality benchmarks of seeds. The use of appropriate germination media can affect the results of seed viability testing. This study aims to determine the optimal germination media in tobacco seed viability testing. This research was conducted at the Seed Laboratory of the Indonesian Sweetener and Fiber Crops Research Institute, Malang in January - March 2019. The research method used a two-factorial randomized block design (RAK). The first factor is tobacco seed varieties consisting of Kasturi in 2007, Bojonegoro in 2012 and Kemloko in 2014. The second factor is the seed germination media consisting of straw paper, cotton, towel tissue, paperboard and newspaper. The results showed that the Kemloko variety of tobacco seeds germinated in the towel tissue had the best seed vigor and viability percentage. Seed germination media with paperboard, towel tissue and straw paper can be used to test the viability of tobacco seeds because they have a percentage of simultaneous growth, percentage of the growth speed, percentage of germination, percentage of the growth potential maximum and normal seedling dry weight which are equally well

Title

Found 5 Documents
Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title Search

Found 5 Documents Search

Abstract

Abstract

Abstract

Abstract

Abstract

Title

Found 5 Documents
Search