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All Journal Ovozoa: Journal of Animal Reproduction Jurnal Kedokteran Hewan
Sri Gustari
Bagian Reproduksi dan Kebidanan, Fakultas Kedokteran Hewan, Universitas Gajah Mada (UGM)
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Veterinary

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Pengaruh penggunaan madu sumbawa dan madu olahan berbagai konsentrasi terhadap kualitas spermatozoa epididimis domba setelah penyimpanan dalam refrigerator Ludfiyaningrum, Siti Diyani; Gustari, Sri
Ovozoa: Journal of Animal Reproduction Vol. 10 No. 1 (2021): Ovozoa: Journal of Animal Reproduction
Publisher : Universitas Airlangga

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20473/ovz.v10i1.2021.1-6

Abstract

Artificial Insemination is used to conserve genetic resources. It is necessary to preserve spermatozoa in a certain diluent at certain temperature before artificial insemination. Information regarding the use of honey in the spermatozoa diluent is still less in number. This research aims to determine the effect of Sumbawa honey and processed honey in various concentrations on the quality of spermatozoa after storage in refrigerator. The main materials used were five ram testicles. Spermatozoa was collected from cauda epididymis by mincing and adding 5 ml PBS. The sample were divided into 7 groups of treatment: 1% Sumbawa honey, 2% Sumbawa honey, 3% Sumbawa honey, 1% processed honey, 2% processed honey, 3% processed honey, and control using 0.5 gram glucose in 100 ml Tris diluent. The assessment was carried out microscopically including motility and viability. Data were analyzed statistically using one-way analysis of variance (Anova) on the SPSS statistics 16.0 program. The results showed no significant difference (p > 0.05) in the motility and viability of spermatozoa in all treatment of Sumbawa honey (1%, 2%, 3%), processed honey (1%, 2%, 3%), and control. The conclusion of this study is that honey in spermatozoa diluent has no effect on the quality of spermatozoa.  
PENGARUH PEMBERIAN MINYAK JINTAN HITAM (Nigella sativa L.) TERHADAP PENINGKATAN SPERMATOGENESIS TIKUS WISTAR YANG TERPAPAR ASAP ROKOK Musfiroh, Mujahidatul; Gustari, Sri
Jurnal Kedokteran Hewan Vol 9, No 2 (2015): September
Publisher : Universitas Syiah Kuala

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21157/j.ked.hewan.v9i2.2820

Abstract

Penelitian ini bertujuan mengetahui efek minyak jintan hitam (Nigella sativa L.) terhadap spermatogenesis tikus Wistar yang terpapar asap rokok. Jenis penelitian adalah eksperimental murni dengan subyek 24 ekor tikus yang terpapar asap rokok selama 21 hari dan dirandomisasi dalam 4 kelompok yaitu kelompok kontrol dan kelompok perlakuan dengan pemberian minyak jintan masing-masing 0,1; 0,2; dan 0,3 ml. Penegakan hipotesis dilakukan dengan menggunakan Krusskal-Wallis dan Mann Whitney dengan batas derajat kemaknaan P0,05, interval kepercayaan 95%. Pemberian minyak jintan hitam dapat memperbaiki derajat spermatogenesis.
Co-Authors Ludfiyaningrum, Siti Diyani Mujahidatul Musfiroh