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All Journal MARINA CHIMICA ACTA
Sarni Sarni
Chemistry department, Faculty of mathematics and Natural Sciences, Hasanuddin University
Biochemistry, Genetics & Molecular Biology

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PRODUCTION AND CHARACTERIZATION CHITOSANASE OF SPONGE Sarni Sarni
Marina Chimica Acta Vol. 17 No. 1 (2016): Radioactivity, Sponge Microsymbiont to degrade sludge oil, Honey as part of Sp
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (251.353 KB) | DOI: 10.20956/mca.v17i1.937

Abstract

Chitosanase enzyme is an enzyme to catalyze the bonding endohidrolisis (1.4) β- glucoside on chitosan into a series chitosan oligomers or chitooligosaccarides. Chitosanase of microbes give good results in producing chitooligosaccarides, but very expensive for use in large-scale industry. This study aims to manufacture and characterization of crude chitosanase enzyme from Klebsiella sp used to hydrolyze chitosan into chitooligosaccarides. Chitosanase produced in the fermentation medium containing 0.5% colloidal chitosan for 60 hours with the activity of 0.309 U / mL (5,235 U / mg), work optimally at 40 ° C and pH 8, is stable at pH 6-7, activated by Co2 + ; Ca 2+ and Ni 2+. Chitooligosaccarides obtained in the form of a mixture of monomer to octamer, which soluble in acetic acid 0.25% to 0.5%, having intrinsic viscosity decreases with increasing time of incubation with molecular weight range of 4103.12 g / mol (incubation 1 hour) ; 1483.48 g / mol (incubation 2 hours) and 1065.79 g / mol (incubation 3 hours).Key word : Chitosanase, Klebsiella, Chitooligosaccarides
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