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All Journal MARINA CHIMICA ACTA

Alfian Noor

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Hasanuddin University

Author-ID : 2734318

Biochemistry, Genetics & Molecular Biology

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MOLECULAR CHARACTERIZATION OF GENE 16S rRNA MICRO SYMBIONTS Ismail Marzuki; Alfian Noor; Nursiah La Nafie; M Natsir Djide
Marina Chimica Acta Vol. 16 No. 1 (2015): Enzyme from Bacteria and Coral Age
Publisher : Hasanuddin University

Molecular characterization studies have been conducted 16S rRNA gene micro symbiont of sponge origin Melawai Beach, Balikpapan in East Kalimantan. Objective analysis of histomorphological research, isolation-purification, molecular characterization of micro-symbiont genes in order to search symbiont bacteria that can live in extreme environments contaminated hydrocarbon waste. The research method that morphological identification, isolation-purification and molecular characterization of the 16S rRNA gene with Chain Reaction Polymerization method. The results of histo-morphological analysis concluded sponge samples with species of Callyspongia sp Isolation and purification mikro symbionts of sponge obtained 2 (two) isolates. Characteristics of Isolates 1; spherical shape, colonize and creamy, while isolates 2; jagged shape, oval and white colonies. Molecular characterization of the 16S rRNA gene by PCR, Bacillus subtilis strain BAB-684 identification for isolates one is the number of nucleotide pairs reached 899 bp and the degree of similarity in GenBank reached 89% homologous, while the second is a Bacillus flexus strain PHCDB20 isolates the number reached 950 bp nucleotide pairs with the degree of similarity in GenBank reached 99% homologous.Keywords: Callyspongia sp, micro-symbionts, characterization, gene Molecules

DETERMINATION OF KOH EFFICIENCY AS CARBOSORB IN DETERMINING AGE OF CORAL REEF SAMPLES IN SELAYAR ISLANDS THROUGH LSC METHOD (LIQUID SCINTILLATION COUNTING) Muhammad Amri; Alfian Noor; Maming Maming
Marina Chimica Acta Vol. 20 No. 1 (2019): Volume 20, No. 1 (2019)
Publisher : Hasanuddin University

Research using the method of LSC (Liquid Scintillation Carbon) in determining the age of coral reefs in the Selayar islands has been carried out. The samples of coral reefs were taken between the tides. Sample preparation was carried out physically and chemically using an acid-base mixture solution: NaOH, H2O2, HClO4 and HCl produced coral samples that looked whiter with weight reduction of up to 1.6%. Carbonate is separated into CO2 by reaction with 10% HCl and absorption with KOH 1 M as K2CO3. Total carbon is determined by the titration method which produces 0.0542 g of total carbon sample. Specific activity of 14C measured by enumeration with LSC is 12.0542 DPM/gC. The age of coral reef samples obtained from sample specific activity data was 1971,728 years

DETERMINATION OF AGE OF CORAL REEFS ON PANIKIANG ISLAND, BARRU DISTRICT, SPERMONDE ISLANDS THROUGH MEASUREMENT ACTIVITY 14C USING LSC (LIQUID SCINTILLATION COUNTING) METHOD Arisandi Arisandi; Alfian Noor; Maming Maming
Marina Chimica Acta Vol. 20 No. 1 (2019): Volume 20, No. 1 (2019)
Publisher : Hasanuddin University

Determination Of Age Of Coral Reefs On Panikiang Island, Barru District, Spermonde Islands Through Measurement Activity 14C Using LSC (Liquid Scintillation Counting) Method. This research was using a sample of coral reefs taken from the Panikiang Island, Barru, Spermonde Islands. This research has been carried out by using sample preparation with physic and chemical. Sample preparation was done physically and chemically. Chemical preparation was carried out by using a mixture of NaOH with 30% H2O2 followed by a mixture of HClO4 with 30% H2O2, and finally with HCl solution to produce a clean sample with a weight reduction of 7.94%. Absorption of CO2 through reaction with HCl 10% and NH4OH as Carbosorb .produced K2CO3. The total carbon in the sample solution is 0.2496 gram sobtained through titration method. Radiocarbon dating method based on the measurement of the specific activity of the samples obtained from the results of counts LSC (Liquid Scintilation Counter) Hidex 300 SL.The specific activity of coral sample is 14.68 ± 4.04 DPM/gC. Age of coral sample which were calculated from the specific activity were 342.04 ± 37.66 years.

THE POTENTIAL BIODEGRADATION HYDROCARBONS OF PETROLEUM SLUDGE WASTE BY CELL BIOMASS SPONGE Callysppongia sp. Ismail Marzuki; Alfian Noor; Nursiah La Nafie
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

The toxicity of petroleum sludge is a serious threat to marine life. Necessary concrete stepspetroleum contamination reduction through a search of potential marine materials degradetoxic components hydrocarbons. The sponge is one potential material reducing toxicproperties of petroleum contamination. The research objective was to determine thepotential Callyspongia sp sponge against the degradation of petroleum hydrocarbonsludge. Four variations of treatment used to determine the potential of sponge biomass,namely: the treatment I, II, III and IV. Indicators degradation is the formation of gas, thesmell fermentation, pH changes and increase in the absorbance of the suspension of mediadegradation. The level degradation each treatment is determined gravimetric method andthe known hydrocarbon component changes by using GC-MS. Unidentified gas, the smellof fermentation and changes in media of pH on average degradation occurs on the 15 daysof contact for all treatments. Data showed degradation occurs absorbance maximum at 20-25 days to contact. The highest degradation rates indicated by IV treatment (26.93 %), III(24.84 %), II (22.59 %), and I: (18.20 %). Found 20 kinds of components in the wastesludge aliphatic petroleum to form a homologous series nC10-nC30, and two aromaticcomponents, namely the 2.7-dimethyl naphthalene and 1.4-dimethyl azulene. Callyspongiasp sponge biomass concluded at the highest IV treatment degrading aliphatichydrocarbons, then treatment III, II and I, while the aromatic components are not degradedby biomass Callyspongia sp sponge at all treatments.

Co-Authors Arisandi Arisandi Ismail Marzuki Ismail Marzuki M Natsir Djide Maming Maming Muhammad Amri Nursiah La Nafie

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