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All Journal MARINA CHIMICA ACTA

Ahyar Ahmad

Chemistry Department, Faculty Of Mathematic And Natural Science , Hasanuddin University, South Sulawesi

Author-ID : 2734347

Biochemistry, Genetics & Molecular Biology

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ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE EXTRACT FROM RED ALGAE Eucheuma cottonii AND Eucheuma spinosum Muawanah Muawanah; Ahyar Ahmad; Hasnah Natsir
Marina Chimica Acta Vol. 17 No. 2 (2016): Volume 17 No. 2 (2016)
Publisher : Hasanuddin University

Red algae Eucheuma cottonii and Eucheuma spinosum which one alternative source of antioxidant and anticancer because contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity polysaccharide extract from red algae Eucheuma cottonii and Eucheuma spinosum . The study was carried out by isolating the polysaccharide extract using water and methanol-ethanol precipitation. Antioxidant activity of crude extract was examined using 1,1-Difenil-2-pikrilhidrazil (DPPH) method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results showed that the crude extract polysaccharide for Eucheuma cottonii and Eucheuma spinosum have strong antioxidant activity with IC50 value of 72,49 ppm and 75,98 ppm. The result of BSLT assay showed that the crude extract polysaccharide has a highest toxicity with LC50 value of 165,88 ppm and 337,21 ppm there are classified as toxic. The crude extract polysaccharide Eucheuma cottonii and Eucheuma spinosum has a potential to be developed as an alternative antioxidant and anticancer agent.

EFFECT OF HEATING AND STORAGE TIME LEVELS 5- (Hydroxy Methyl) furan-2- KARBALDEHIDA (HMF) IN HONEY ORIGIN MALLAWA Suhaela Suhaela; Alfian Noor; Ahyar Ahmad
Marina Chimica Acta Vol. 17 No. 2 (2016): Volume 17 No. 2 (2016)
Publisher : Hasanuddin University

A research has been on determining the origin of forest honey quality Mallawa Maros based on the levels of 5-(hydroxymethyl)furfural-2-karbaldehida (HMF)is quantitatively based on the variation of heating temperature and duration of storage with White spectrophotometric method. The results showed that the levels of forest honey HMF origin Mallawa on heating temperature 35°C,90°C and 110°C respectively is 42.2023 mg/Kg, 46.0274 mg/Kg and 62.2520 mg/Kg. While the storage duration for 3,4, and 5 months had higher levels of HMF each 27.4309 mg/Kg, 42.5158 mg/Kg and 48.9405 mg/Kg. Heating temperature and storage time a big influence on levels of HMF and show a strong relationship to the water content, pH and free acid, reducing sugar content and diastase enzyme activity. Based on the research results, levels of HMF at the heating temperature of 110oC has passed the standards set by the SNI (up to 50 mg/Kg). Whereas the standard set by IHC for honey originating from thetropics (80 mg/ Kg)

TOXICITY ASSESSMENT ON NINE SPONGES SPECIES FROM SPERMONDE ARCHIPELAGO Ajuk Sapar; Alfian Noor; Nunuk Hariani Soekamto; Ahyar Ahmad; Tri Aryono Hadi
Marina Chimica Acta Vol. 16 No. 2 (2015): Volume 16 No. 2 (2015)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mca.v16i2.2542

Toxicity assessment have been conducted on nine sponges species collected from Spermonde Archipelago namely Kapoposang Island, Samalona Island and Lanjukang Island. Toxicity assessment was using Brine Shrimp lethality Test (BST) method againts sponge extracts. Theresults test were expressed as 50% lethal concentration (LC50) to Artemia salina and correlated with prospecting extract potential as anticancer. All extracts obtained from smallscale extraction by methanol. Test results as follows: Acanthostrongylophora ingens (24.830 μg/mL), Hytios erectus (46.140 μg/mL), Stylissa massa (>1000 μg/mL), Theonella sp (35.010 μg/mL), Niphates olemda (87.500 ppm), Haliclona (Reniera) fascigera (78.160 μg/mL), Petrosia (Petrosia) sp (3.008), Callyspongia (Euplacella) biru (0.515 μg/mL). The test results showed that seven species are potential as anticancer with LC50 value ≤ 500 ppm and only Stylissa massa extracts was not potential as anticancer.

PURIFICATION, ANTIOXIDANT ACTIVITY AND TOXICITY POLYSACCHARIDE FRACTION FROM RED ALGAE Gracilaria verrucosa Muawanah Muawanah; Ahyar Ahmad; Hasnah Natsir
Marina Chimica Acta Vol. 18 No. 2 (2017): Volume 18 No. 2 (2017)
Publisher : Hasanuddin University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.20956/mca.v18i2.2688

it contains polysaccharide compound. This research aims to isolate and examine the activity of antioxidant and toxicity of polysaccharide extract and fraction fromred algae Gracilariaverrucosa. The study was carried out by isolating the polysaccharide extract using aqueous 100 o C and methanol-ethanol precipitation .Purification of polysaccharide done by Column Chromatography DEAE-Cellulose and analysis polysaccharide concentration was using phenol-sulfuric acid. Antioxidant activity of crude extract and polysaccharide fraction was examined using DPPH method, and toxicity test was carried out using Brine Shrimp Lethality Test (BSLT). The research results reveals that the highest polysaccharide concentration was fraction 7 with value of 0,267 mg/mL, and the crude extract and fraction 4 have strongest antioxidant activity with IC50 values of 51,22 and 62,25 ppm, respectively. The result of BSLT assay showed that the crude extract and fraction 6 has a highest toxicity with LC50 values of 47,73and 58,48 ppm, therefore it is classified as toxic. The polysaccharide of crude extract and fraction 7 has a potential to be developed as an alternative antioxidant and antitumor agent. Identification with FT-IR shows the characteristic bands of agarocolloid.

Bioactivity of Protein Fraction in Brown Algae, Turbinaria decurrens, as Antibacterial Agent Bioaktivitas Fraksi Protein dalam Alga Coklat, Turbinaria Decurrens, sebagai agen anti bakteri Seniwati Dali; Hasnah Natsir; Hanapi Usman; Ahyar Ahmad
Marina Chimica Acta Vol. 14 No. 1 (2013): Volume 14 No. 1 (2013)
Publisher : Hasanuddin University

Penelitian ini bermaksud mengeksplorasi dan mengkarakterisasi fraksi protein dari alga cokelat Turbinaria decurrens di Sulawesi Selatan. Senyawa protein bioaktif diperoleh melalui serangkaian proses ekstraksi, fraksionasi dengan kejenuhan amonium sulfat 0-20%, 20-40%, 40-60%, dan 60-80% yang dilanjutkan dengan proses dialisis lalu ditentukan kadar proteinnya dengan metode Lowry. Fraksi protein yang diperoleh diuji bioaktivitasnya terhadap bakteri Staphylococcus aureus dan Salmonella thypi dengan metode difusi agar pada medium MHA. Hasil penelitian menunjukkan bahwa kadar protein tertinggi ditemukan pada fraksi 0-20% yaitu sebesar 5,1 mg/mL. Hasil uji bioaktivitas antibakteri terbesar ditemukan pada fraksi protein 0-20% terhadap Staphylococcus aureus dan Salmonella thypi dengan zona daerah hambatan masing-masing sebesar 18,33 mm dan 13,30 mm yang bersifat bakteriostatik. Hasil penelitian awal ini akan dikembangkan pada cakupan penelitian yang lebih luas untuk mengkloning protein bioaktifnya dan uji pre-klinis dari protein antibakteri yang diperoleh.

ANALYSIS OF DIMETHYLSULFIDE AS BIOGEOCHEMICAL CYCLES SULFUR ON REGIONAL ESTUARY SPERMONDE Rakhmad Armus; Alfian Noor; Ahyar Ahmad; Muhammad Lukman
Marina Chimica Acta Vol. 19 No. 1 (2018): Volume 19, No. 1 (2018)
Publisher : Hasanuddin University

We developed a more accurate method of analysis for low-concentration volatile compounds used for the determination of free dimethylsulfide (DMS) in marine phytoplankton by using solid phase (SPME) gas chromatography - spectrometry Mass of GC-MS and head space method. DMSP, as a product of bacteria degradation has been scientifically recognized to be affected by climate change due to increased in sea surface temperature, and form DMS compunds.. The present study is to determine the DMSP concentration and degradation pathway in the oligotrophic region of Spermonde waters. The spatial and temporal correlation between DMSP degrading bacterial codes and biological potential controls for DMS formation in the open seas was examined using a statistical factorial analysis of variance and regression against the spatial (or distance) gradients of the Spermonde coastal waters, the concentration of chlorophyll-a, and diatom community structures. Seawater chemical properties, chlorophyll-a, and diatom samples were collected from several sites in the Tallo-Makassar river estuary, and the Pangkep river in September 2017 (transitional wet-to-dry-season). The result shows that the concentration of the Domination Index was higher in the transition season of 35.2 to 85.2 μM than in other seasons. The abundance of diatoms during the transition season reached 16,534 plankter / mL.

Co-Authors Ajuk Sapar Alfian Noor Alfian Noor Alfian Noor Armus, Rakhmad Hanapi Usman Hasnah Natsir Hasnah Natsir Hasnah Natsir Muawanah Muawanah Muhammad Lukman Nunuk Hariani Soekamto Seniwati Dali Suhaela Suhaela Tri Aryono Hadi

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