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Isolasi dan Karakterisasi Cendawan Dekomposer pada Bahan Kompos Jerami, Endapan Tanah Danau Tempe dan Tanah Exfarm Pertanian Universitas Hasanuddin Fatmawati Fatmawati; Burhanuddin Rasyid; Muh. Jayadi
Jurnal Ecosolum Vol. 7 No. 2 (2018): DESEMBER
Publisher : Universitas Hasanuddin

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (708.225 KB) | DOI: 10.20956/ecosolum.v7i2.6879

Abstract

Agricultural wastes able to provide profit value if utilized properly for example made into organic fertilizer. Organic fertilizer production requires microbial decomposers to help the decomposition process. This research aims to isolate and identify the characteristics of microbial decomposers found in straw compost, soil sediment from tempe lake and soil from exfarm. In this research, conducted microbial isolation. Microbial exploration of compost material, soil organic matter and soil deposition material is mixed with physiological solution then grown on PDA, further purification of the unwanted microbes. In this study only used the media to grow the asparagine fungus to see the growth of macroscopic and microscopic fungi. The results showed that there are seven isolates of the fungus from different material extraction. The extracted compost material to produce two fungi are Aspergillus sp. and Penicillium sp., Tempe lake sediment soil shows three types of fungi with Aspergillus Sp. and two types Zygomisota, while soil from Exfarm also shows two types of fungi are Aspergillus sp. and Penicillium sp. The growth rate of each different fungal isolates based sources although of the same type
SELEKSI BAKTERI METHANOTROF (PEREDUKSI EMISI GAS METAN DI LAHAN SAWAH) BERDASARKAN AKTIVITAS ENZIM METHAN MONOOKSIGENASE Maimuna Nonci; Baharuddin Baharuddin; Burhanuddin Rasyid; Pirman Pirman
Jurnal Ilmu Lingkungan Vol 13, No 2 (2015): Oktober 2015
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.151 KB) | DOI: 10.14710/jil.13.2.87-91

Abstract

Kehadiran bakteri metanotrof pada daerah rhizosfer padi sangat dibutuhkan untuk mereduksi metan yang dihasilkan oleh bakteri metanogen, sehingga tidak terjadi emisi gas metan ke atmosfer. Penelitian ini bertujuan untuk melakukan koleksi dan seleksi bakteri methanotrof asal rhizosfer tanaman padi yang mampu menghasilkan enzim metan monooksigenase. Pengambilan sampel dilakukan secara acak pada tiga fase pertumbuhan tanaman padi yaitu fase vegetatif, reproduktif dan pematangan. Koleksi isolat dilakukan dengan metode isolasi dan pemurnian. Seleksi isolat dilakukan dengan pengujian reaksi gram, motil, indol, aerob/anaerob dan aktivitas enzim monooksigenase dianalisis dengan metode kolorimetrik.  Hasil penelitian diperoleh 52 koleksi isolat bakteri. Berdasarkan reaksi gram terseleksi 22 isolat gram negatif, berdasarkan analisis kolorimetrik  diperoleh 10 isolat  menghasilkan enzim metan monooksigenase.