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All Journal Jurnal Ilmu Lingkungan Jurnal Agercolere
Pirman Pirman
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Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Economics, Econometrics & Finance Environmental Science Social Sciences

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SELEKSI BAKTERI METHANOTROF (PEREDUKSI EMISI GAS METAN DI LAHAN SAWAH) BERDASARKAN AKTIVITAS ENZIM METHAN MONOOKSIGENASE Maimuna Nonci; Baharuddin Baharuddin; Burhanuddin Rasyid; Pirman Pirman
Jurnal Ilmu Lingkungan Vol 13, No 2 (2015): Oktober 2015
Publisher : School of Postgraduate Studies, Diponegoro Univer

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (320.151 KB) | DOI: 10.14710/jil.13.2.87-91

Abstract

Kehadiran bakteri metanotrof pada daerah rhizosfer padi sangat dibutuhkan untuk mereduksi metan yang dihasilkan oleh bakteri metanogen, sehingga tidak terjadi emisi gas metan ke atmosfer. Penelitian ini bertujuan untuk melakukan koleksi dan seleksi bakteri methanotrof asal rhizosfer tanaman padi yang mampu menghasilkan enzim metan monooksigenase. Pengambilan sampel dilakukan secara acak pada tiga fase pertumbuhan tanaman padi yaitu fase vegetatif, reproduktif dan pematangan. Koleksi isolat dilakukan dengan metode isolasi dan pemurnian. Seleksi isolat dilakukan dengan pengujian reaksi gram, motil, indol, aerob/anaerob dan aktivitas enzim monooksigenase dianalisis dengan metode kolorimetrik.  Hasil penelitian diperoleh 52 koleksi isolat bakteri. Berdasarkan reaksi gram terseleksi 22 isolat gram negatif, berdasarkan analisis kolorimetrik  diperoleh 10 isolat  menghasilkan enzim metan monooksigenase.
Ekstraksi albumin ikan gabus (Channa striata) pada titik isoeletriknya Muhammad Asfar; Abu Bakar Tawali; Pirman Pirman; Meta Mahendradatta
Jurnal Agercolere Vol 1 No 1 (2019): Jurnal Agercolere
Publisher : Fakultas Pertanian Universitas Ichsan Gorontalo

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (215.443 KB) | DOI: 10.37195/jac.v1i1.55

Abstract

The high content of albumin in snakehead fish and proof of efficacy in clinical trials against several diseases, as well as the expensive commercial albumin preparations, making a snakehead fish alternative as a cheap source of albumin. The purpose of this study was to optimize the extraction, purification of albumin from snakehead fish to obtain higher levels of albumin. For that need to be investigated to obtain the isoelectric point of albumin extract with the greatest yield. Treatment research is the use of solvents is 0.9% NaCL and dilute HCL and extraction is by heating and without heating. The parameters tested were the determination of the isoelectric point, moisture content, albumin, and yield. The results obtained showed that the isoelectric point of albumin is at pH 4.6 with 62.9% albumin, 7.8% moisture content and yield of 11.6%.
Co-Authors Abu Bakar Tawali Baharuddin Baharuddin Burhanuddin Rasyid Maimuna Nonci Meta Mahendradatta Muhammad Asfar