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Hanif Yuliani
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Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry

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The Initial Dioxigenase Gene Squences Analysis of Marine Bacteria Strain M128 Muhamad Sahlan; Hanif Yuliani; Heri Hermansyah; Anondho Wijanarko
ASEAN Journal of Chemical Engineering Vol 13, No 1 (2013)
Publisher : Department of Chemical Engineering, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (953.401 KB) | DOI: 10.22146/ajche.49723

Abstract

Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzed by multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAc gene amplification. The nahAc gene commonly used as biomarkers of PAH degradation, and as a result, nahAc gene sequence analysis of marine bacteria M128 strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.
Co-Authors Anondho Wijanarko Heri Hermansyah SAHLAN, MUHAMAD