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All Journal HAYATI Journal of Biosciences Microbiology Indonesia Dharmakarya : Jurnal Aplikasi Ipteks Untuk Masyarakat Communications in Science and Technology Jurnal Ilmu Kefarmasian Indonesia ASEAN Journal of Chemical Engineering International Journal of Social Service and Research Makara Journal of Technology Journal of Life Science and Technology
SAHLAN, MUHAMAD
Department Of Chemical Engineering, Faculty Of Engineering, Universitas Indonesia, Kampus UI Depok 16424, Indonesia. Research Center For Biomedical Engineering, Faculty Of Engineering, Universitas Indonesia, Kampus UI Depok 16424, Indonesia
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Propolis Components and Biological Activities from Stingless Bees Collected on South Sulawesi, Indonesia Ryo Miyata; Muhamad Sahlan; Yoshinobu Ishikawa; Hiroshi Hashimoto; Sari Honda; Shigenori Kumazawa
HAYATI Journal of Biosciences Vol. 27 No. 1 (2020): January 2020
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (432.47 KB) | DOI: 10.4308/hjb.27.1.82

Abstract

Three new compounds, namely sulabiroins A (1) and B (2), and 2',3'-dihydro-3'-hydroxypapuanic acid (3), were isolated from the propolis of stingless bees (Tetragonula aff. biroi) collected on South Sulawesi, Indonesia. In addition, ten known compounds, (–)-papuanic acid (4), (–)-isocalolongic acid (5), isopapuanic acid (6), isocalopolyanic acid (7), glyasperin A (8), broussoflavonol F (9), (2S)-5,7-dihydroxy-4'-methoxy-8-prenylflavanone (10), isorhamnetin (11), (1'S)-2-trans,4-trans-abscisic acid (12), and (1'S)-2-cis,4-trans-abscisic acid (13) were identified. The structures of the new and known compounds were determined by spectroscopic analysis. The absolute configurations of sulabiroins A (1) and B (2) were determined by X-ray crystallography analysis and ECD calculation, respectively. The propolis from stingless bee (Tetragonula aff. biroi) collected on South Sulawesi contained compounds not present in propolis from other regions. Sulabiroin A (1) and isorhamnetin (11) were examined for xanthine oxidase (XO) inhibitory activity as one of biological activities; isorhamnetin (11) exhibited potent XO inhibitory activity, with an IC50 value of 3.9 µm.
PEMANFAATAN LEBAH DAN PENGELOLAAN SAMPAH UNTUK EKONOMI RUMAH TANGGA YANG BERKELANJUTAN Afiatry Putrika; Deti Purwanti; Adra Amalia Nur Ahlina; Nadhifa Tazkia Ramadhani; Ratna Yuniati; Muhamad Sahlan; Kenny Lischer; Apriliana Cahya Khayrani; Amy Yayuk Sri Rahayu; Nidaan Khafian; Astari Dwiranti
Dharmakarya Vol 10, No 3 (2021): September, 2021
Publisher : Universitas Padjadjaran

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24198/dharmakarya.v10i3.32506

Abstract

Lebah merupakan hewan dari kelas Insecta yang menghasilkan produk kaya manfaat, diantaranya madu, propolis, dan bee pollen. Lebah, khususnya yang tanpa sengat, dapat dibudidayakan oleh masyarakat perkotaan karena relatif aman. Tim Pengabdian Masyarakat Multidisiplin Universitas Indonesia, yang terdiri dari Fakultas Matematika dan Ilmu Pengetahuan Alam, Fakultas Teknik, dan Fakultas Ilmu Administrasi bekerjasama dengan komunitas “3bee” telah melaksanakan serial workshop secara daring dengan topik pemanfaatan lebah. Selain itu, tim pengabdi dengan “holistika institute” juga mengajak masyarakat untuk memanfaatkan sampah rumah tangga agar dapat diolah kembali menjadi produk yang dapat menghidupkan perekonomian rumah tangga. Serial workshop terdiri dari empat seri, masing-masing terdiri dari dua sesi. Topik yang disampaikan meliputi pengenalan lebah dan urban bee; tanaman pakan lebah dan produk lebah; pangan sehat dari lebah; pengolahan sampah organik dan anorganik; serta pemasaran dan kualitas pelayanan. Workshop diselenggarakan selama 4 Pekan mulai tanggal 10 Oktober hingga 7 November 2020. Selain pemaparan narasumber, terdapat pre-test, post-test, dan evaluasi kegiatan dari peserta pada tiap seri. Peserta workshop berjumlah 171 orang yang berasal dari berbagai wilayah di Indonesia. Sebagian besar berasal dari kalangan akademisi, komunitas penggiat lebah, serta pegawai dinas lingkungan dan kehutanan. Berdasarkan hasil evaluasi, sebagian besar peserta merasa sangat puas (rentang kepuasan 4,21-5,00) dengan workshop yang terselenggara dan berencana mengimplementasikan pengetahuan yang diperoleh dari workshop ini (87%). Program pengabdian masyarakat multidisiplin ini dapat diduplikasi pada program lain sehingga penyampaian materi bersifat komprehensif. Program ini diharapkan dapat terus dikembangkan melalui program pendampingan sehingga lebah dan sampah dapat dimanfaatkan untuk meningkatkan ekonomi rumah tangga berkelanjutan di Indonesia.
Cultivation Process Optimization Of Recombinant Bacillus Subtilis Apoptin MUHAMAD SAHLAN; YUKI DESIANDINI; MUHAMMAD IQBAL; NUNUK WIDHYASTUTI; AMARILA MALIK; SISWA SETYAHADI
Microbiology Indonesia Vol. 7 No. 4 (2013): November 2013
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (282.881 KB) | DOI: 10.5454/mi.7.4.3

Abstract

The success of recombinan apoptin production in native form in the previous results open the way to develop this anticancer protein production to the larger scale. We optimized cultivation process of recombinant Bacillus subtilis 168 harbouring pOXGW12His8Arg with apoptin gene in a stirred tank fermentor and shake flasks. The parameters to optimize cultivation process are xylose-inducer concentration, agitation speed, and aeration rate. The xylose-inducer concentration variations are carried out in a shake flasks with 100 mL volume broth, while the agitation speed and aeration rate variation is carried out in a stirred tank fermentor with 3 L volume broth. The xylose concentration is varied between 0-5% w/v, while agitation speed and aeration rate are varied between 150-250 rpm and 0.5-1.5 NL min-1 respectively. The best condition in this cultivation is 1% w/v of xylose, 250 rpm of agitation speed and 1,5 NL min-1 of aeration rate giving the specific growth rate value for each parameter of 0.628 h-1; 0.630 h-1; and 0.747 h-1 respectively. The recombinant apoptin were purified by Ni-NTA column using AKTA system. However, the results showed that the optimum condition of producing recombinant apoptin was 1% w/v of xylose, 250 rpm of agitation speed, and 0.5 NL min-1 of aeration.
The Initial Dioxigenase Gene Squences Analysis of Marine Bacteria Strain M128 Muhamad Sahlan; Hanif Yuliani; Heri Hermansyah; Anondho Wijanarko
ASEAN Journal of Chemical Engineering Vol 13, No 1 (2013)
Publisher : Department of Chemical Engineering, Universitas Gadjah Mada

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (953.401 KB) | DOI: 10.22146/ajche.49723

Abstract

Biodegradation of polyaromatic hydrocarbons (PAHs) are catalyzed by multicomponent enzymes from microbe. The initial dioxygenase was used as a key enzyme for attacking the aromatic ring structure of PAHs, furthermore its initial dioxygenase gene was used to select PAHs degrading bacteria. Marine bacteria M128 strain could grow on medium contained PAHs. Detection of its cellular initial deoxygenase gene was done by nahAc gene amplification. The nahAc gene commonly used as biomarkers of PAH degradation, and as a result, nahAc gene sequence analysis of marine bacteria M128 strain was similar to naphthalene dioxygenase of Pseudomonas genera with 99% homology.
Expression and the Functional Study of Fusion Proteins α-Amylase and Hemolysin- αas an Application in Biofilm Polysaccharide Degradation Sugiarta, Gede Yuda; Wiseso, Anggoro; Sari, Siska Yuliana; Kamila, Etri Dian; Geraldine, Vanessa; Christina, Diana; Hanifi, Muhammad; Satyapertiwi, Dwiantari; Hertanto, Robby; Bela, Budiman; Yohda, Masafumi; Sahlan, Muhamad
Makara Journal of Technology Vol. 20, No. 3
Publisher : UI Scholars Hub

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Abstract

Biofilm is an aggregate of consortium bacteria that adhere to each other on a surface. It is usually protected by the exopolysaccharide layer. Various invasive medical procedures, such as catheterization, endotracheal tube installation, and contact lens utilization, are vulnerable to biofilm infection. The National Institute of Health (NIH) estimates 65% of all microbial infections are caused by biofilm. Periplasmic α-amylase (MalS) is an enzyme that hydrolyzes α-1, 4- glicosidic bond in glycogen, starch, and others related polysaccharides in periplasmic space. Another protein called hemolysin-α (HlyA) is a secretion signal protein on C terminal of particular peptide in gram negative bacteria. We proposed a novel recombinant plasmid expressing α-amylase and hemolysin-α fusion in pSB1C3 which is cloned into E.coli to enable α-amylase excretion to extracellular for degrading biofilm polysaccharides content, as in starch agar. Microtiter assay was performed to analyze the reduction percentage of biofilm by adding recombinant E.coli into media. This system is more effective in degrading biofilm from gram positive bacteria i.e.: Bacillus substilis (30.21%) and Staphylococcus aureus (24.20%), and less effective degrading biofilm of gram negative i.e.: Vibrio cholera (5.30%), Pseudomonas aeruginosa (8.50%), Klebsiella pneumonia (6.75%) and E. coli (-0.6%). Gram positive bacteria have a thick layer of peptidoglycan, causing the enzyme to work more effectively in degrading polysaccharides.
Encapsulation Process of Propolis Extract by Casein Micelle Improves Sunscreen Activity Sahlan, Muhamad; Dienayati, Dara; Hamdi, Darul; Zahra, Soraya; Hermansyah, Heri; Chulasiri, Malyn
Makara Journal of Technology Vol. 21, No. 1
Publisher : UI Scholars Hub

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Abstract

Propolis, a wax bee glue, have antioxidant activity and effective as broad spectrum UVB and UVA photoprotection sunscreens agent. To improve photoprotection activity in sunscreen cream, the propolis was encapsulated by casein micelle. The Indonesian propolis was extracted by ethanol and separated the wax by freeze precipitation. The extract was encapsulated by casein and reduced their size by ball mill homogenizer with the encapsulation efficiency about 80% and size of particles about 80 nm. The particles was applied in sunscreen cream, evaluate their stabillity and photoprotection activity Sun Protection Factor (SPF) spectrophotometer (Optometric 290s). The results shows that the selected creams were stable and have moderate photoprotection activities higher than the controls. The encapsulation process of propolis by casein micelle can improve their photoprotection activity.
Isolation and Molecular Weight Characterization of Tetragonula laeviceps Honey Protein Sahlan, Muhamad; Azizah, Nurul; Hakamada, Kazuaki; Noguchi, Keiichi; Yohda, Masafumi
Makara Journal of Technology Vol. 22, No. 1
Publisher : UI Scholars Hub

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Abstract

The concentration of protein in honey is lower than polysaccharide. However, recently the honey’s protein is intensively studied and it showed that protein also have several biological activities such as antibacterial activity. The purpose of this research is to isolate and characterize Tetragonula laeviceps honey protein by determining the molecular weight. Honey protein of Trigona laeviceps was isolated using ultra-filtration with the membrane’s size of 30 kDa, then concentrated using tube membrane size 10 kDa. Molecular weight was analyzed by SDS PAGE. From the analysis, there was major protein band in honey produced by Tetragona laeviceps identified. The produced molecular weight of major protein bands were about 87 and 65 kDa. Determining of the molecular weight of this protein could be used to detect the originality of Tetragonula laeviceps honey from Indonesia.
Comparison of Total Phenolic Content and Antioxidant Activity of Indonesian Propolis Extracted with Various Solvents. Diah Kartika Pratami; Nur Elistiani Eksadita; Muhamad Sahlan; Abdul Mun’im; Asep Bayu; Kaysa Faradis Mahira
JURNAL ILMU KEFARMASIAN INDONESIA Vol 21 No 1 (2023): JIFI
Publisher : Fakultas Farmasi Universitas Pancasila

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.35814/jifi.v21i1.1400

Abstract

Propolis is a widely used medicine that may be found in both its pure form and when combined with othernatural ingredients in over-the-counter preparations, cosmetics, and health foods. Some customers develop alcohol sensitivity after using ethanol, which is a common solvent to extract propolis. As propolis solvents, various Natural Deep Eutectic Solvents (NaDES) were examined in this study. Choline chloride, propylene glycol, glycerol, citric acid, and 1,2-propanediol were combined in molar ratios to create some of the NaDES that were employed. Propolis and solvents were used in a kinetic maceration ratio for the extraction process. The Folin-Ciocalteu method was used to measure the total phenolic content (TPC), and the DPPH assay was used to measure antioxidant activity. In ethanol and NaDES solvents, the TPC of propolis extract ranged from 136.52 ± 27.9 to 365.8 ± 20.54 mgGAE/g. The values for antioxidant activity were 45.94 to 183.76 ppm. The propolis extract with the lowest IC50 and the highest TPC content was choline chloride-glycerol (CCG). It was discovered that the optimum NaDES solvent for extracting propolis was CCG-based. NaDES may be a potential solvent for use in Indonesian propolis, both as an extraction medium and as a formulation element.
Formulation of Anti-Oral Mouthwash Nanoemulsion Biofilm Based on Propolis Extract Heterotrigona itama, Tetragonula sapiens, and Tetragonula clypearis Muhamad Sahlan; Alya Fadhila Ismail; Diah Kartika Pratami; Apriliana Cahya Khayrani
HAYATI Journal of Biosciences Vol. 30 No. 6 (2023): November 2023
Publisher : Bogor Agricultural University, Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.4308/hjb.30.6.1175-1186

Abstract

The use of mouthwash is one of the actions against biofilms that are often used. However, commercial mouthwashes have a fairly high alcohol content, which is around 26.9% of the total volume, which is considered to have a prolonged impact where high alcohol content in direct contact with the oral mucosa can cause lesions or abnormalities, resulting in a shift in the medical paradigm towards eco-friendly widely considered as a solution. Propolis with antibacterial ability was formulated using the nanoemulsion steps, which were initiated by separating pure propolis through drying, and then there were variations in the formulation of 3 types of bee propolis: Heterotrigona itama, Tetragonula sapiens, and Tetragonula clypearis along with the addition of Tween 80, propylene glycol, glycerin and then the effect on microbial growth of S. mutans was compared with antimicrobial agents in Brazilian propolis with the identification and comparison of the antibacterial activity stability of the organoleptic formula. Where the active ingredient content of propolis is the highest in H. itama propolis with a total flavonoid content of 38.94 mgQE/L sample and T. clypearis propolis has the lowest total flavonoid content of 14.23 mgQE/L sample with its function as an anti-oral biofilm agent by inhibiting the glucosyltransferase was proven with a minimum percentage of 49% inhibition of S. mutans and degradation of 18% with the use of a combined surfactant proved to be able to increase the stability of the preparation shown at 2:1 (v/v).
Analisis Kadar Polifenol Total dan Flavonoid Total Propolis Asal Tanah Laut dan Soppeng Dwi Anggar Yusika; Izzul Islam; Muhamad Sahlan
BIOMARAS : Journal of Life Science and Technology Vol 1 No 1 (2023): BIOMARAS (Vol.1, No.1 Agustus 2023)
Publisher : BIOMARAS : Journal of Life Science and Technology

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Abstract

Propolis biasa dikenal sebagai lem lebah, adalah zat yang dibuat oleh lebah dari resin, serbuk sari, dan lilin tanaman yang dikumpulkan lebah dari berbagai jenis tanaman. Propolis dapat dijadikan sebagai natural produk atau alternatif pengobatan karena memiliki banyak manfaat. Penelitian ini bertujuan untuk mengetahui  kandungan polifenol dan flavonoid total dari sampel propolis Heterotrigona itama, Tetragonula laeviceps dari Tanah Laut, dan Tetragonula sapiens dari Soppeng. Metode penelitian meliputi preparasi sampel, ekstraksi, dan pengujian total kandungan polifenol dan flavonoid dari tiap-tiap sampel propolis. Hasil penelitian menunjukkan bahwa total kandungan polifenol H. itama memiliki nilai 1440,04±353,99 mg GAE/g propolis, T. laeviceps 824,32±59,44 mg GAE/g propolis, dan nilai T. sapiens 786,65±473,45 mg GAE/g propolis. Total kandungan flavonoid T. laeviceps memiliki nilai 434,50±15,89 mg QE/g propolis, H. itama 425,23±24,71 mg QE/g propolis, dan nilai T. sapiens 143,44±6,49 mg QE/g propolis. Dari hasil yang didapatkan disimpulkan bahwa tiap sampel propolis memiliki karakteristik warna yang berbeda serta nilai total kandungan polifenol tertinggi dihasilkan oleh propolis H. itama asal Tanah Laut, sedangkan nilai total kandungan flavonoid tertinggi dihasilkan oleh propolis T. laeviceps asal Soppeng. Perbedaan hasil ekstrak propolis serta nilai total polifenol dan flavonoid tersebut dipengaruhi oleh jenis tanaman yang dijadikan sumber pakan oleh lebah
Co-Authors Abdul Mun’im Adra Amalia Nur Ahlina Alya Fadhila Ismail AMARILA MALIK ANGGI KHAIRINA HANUM HASIBUAN Anondho Wijanarko Araz Meilin Asep Bayu Budiman Bela Christina, Diana Chulasiri, Malyn Deti Purwanti Diah Kartika Pratami Diana, Akso Dienayati, Dara Dwi Anggar Yusika Dwiranti, Astari Geraldine, Vanessa Hakamada, Kazuaki Hamdi, Darul Hanif Yuliani Hanifi, Muhammad Heri Hermansyah Hertanto, Robby Hiroshi Hashimoto Istikomah, Iis izzul islam Kamila, Etri Dian Kaysa Faradis Mahira Khayrani, Apriliana Cahya Kuntadi Kuntadi, Kuntadi Lischer, Kenny MUHAMMAD IQBAL Nadhifa Tazkia Ramadhani Nidaan Khafian Noguchi, Keiichi NUNUK WIDHYASTUTI Nur Elistiani Eksadita NURUL AZIZAH Perdani, Meka Saima Pujianto, Paksi Purwanti, Lucia Purwanto, Didik B. Putri, Dwini Normayulisa Putrika, Afiatry Ratna Yuniati RIKA RAFFIUDIN Ryo Miyata Sari Honda Sari, Siska Yuliana Satyapertiwi, Dwiantari Shigenori Kumazawa SISWA SETYAHADI Siti Farida Sri Rahayu, Amy Yayuk Sugiarta, Gede Yuda Teguh Pambudi Teti Indrawati TRI BASTUTI PURWANTINI Wiseso, Anggoro Yelin Adalina Yohda, Masafumi Yoshinobu Ishikawa YUKI DESIANDINI Zahra, Soraya